Parasites, Hosts and Diseases

"Brugia pahangi"

Original Article

Antifilarial and Antibiotic Activities of Methanolic Extracts of Melaleuca cajuputi Flowers: Nazeh M. Al-Abd, Zurainee Mohamed Nor, Marzida Mansor Hasan, Mustafa Kassim; Korean J Parasitol 2016;54(3):273-280.
Published online June 30, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.3.273

Abstract
PDF

We evaluated the activity of methanolic extracts of Melaleuca cajuputi flowers against the filarial worm Brugia pahangi and its bacterial endosymbiont Wolbachia. Anti-Wolbachia activity was measured in worms and in Aedes albopictus Aa23 cells by PCR, electron microscopy, and other biological assays. In particular, microfilarial release, worm motility, and viability were determined. M. cajuputi flower extracts were found to significantly reduce Wolbachia endosymbionts in Aa23 cells, Wolbachia surface protein, and microfilarial release, as well as the viability and motility of adult worms. Anti-Wolbachia activity was further confirmed by observation of degraded and phagocytized Wolbachia in worms treated with the flower extracts. The data provided in vitro and in vivo evidence that M. cajuputi flower extracts inhibit Wolbachia, an activity that may be exploited as an alternative strategy to treat human lymphatic filariasis.

Citations

Citations to this article as recorded by

Two decades of antifilarial drug discovery: a review
Jaiprakash N. Sangshetti, Devanand B. Shinde, Abhishek Kulkarni, Rohidas Arote
RSC Advances.2017; 7(33): 20628. CrossRef

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Brief Communication

Successful Treatment of Brugia pahangi in Naturally Infected Cats with Ivermectin: Piyanan Taweethavonsawat, Sudchit Chungpivat; Korean J Parasitol 2013;51(6):759-761.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.759

Abstract
PDF

Lymphatic filariasis is a common parasitic disease of cats in tropical regions including Thailand. The
objective
of this study was to determine the efficacy of ivermectin against microfilariae of Brugia pahangi in naturally infected cats. Eight cats naturally infected with B. pahangi were divided into control (untreated) and treated groups. Cats in the latter group were given ivermectin injection at 400 ?g/kg weekly for 2 months. Microfilariae were counted every week until 48 weeks. Microfilaremia was significantly decreased in the treated group 4 weeks after starting the treatment and become zero at week 9 and afterwards. On the other hand, cats in the control group had high microfilaremia throughout the study. It was successful to treat and control B. pahangi infection in naturally infected cats using ivermectin.

Citations

Citations to this article as recorded by

Filariasis of the breast caused by Brugia pahangi: A concomitant finding with invasive ductal carcinoma
Jerapas Thongpiya, Doonyapat Sa-nguanraksa, Norasate Samarnthai, Patsharaporn T. Sarasombath
Parasitology International.2021; 80: 102203. CrossRef
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Poojary Vineeta Sadarama, Deepa Chirayath, Usha Narayana Pillai, Bindu Lakshmanan
Journal of Parasitic Diseases.2019; 43(4): 554. CrossRef
Therapeutic trial of doxycyclin plus ivermectin for the treatment of Brugia malayi naturally infected cats
Ladawan Khowawisetsut, Patsharaporn T. Sarasombath, Suwich Thammapalo, Sumart Loymek, Therayot Korbarsa, Hathai Nochote, Achinya Phuakrod, Wej Choochote, Sirichit Wongkamchai
Veterinary Parasitology.2017; 245: 42. CrossRef

7,661 View
88 Download
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Original Article

Rapid Detection and Identification of Wuchereria bancrofti, Brugia malayi, B. pahangi, and Dirofilaria immitis in Mosquito Vectors and Blood Samples by High Resolution Melting Real-Time P: Tongjit Thanchomnang, Pewpan M. Intapan, Chairat Tantrawatpan, Viraphong Lulitanond, Sudchit Chungpivat, Piyanan Taweethavonsawat, Worasak Kaewkong, Oranuch Sanpool, Penchom Janwan, Wej Choochote, Wanchai Maleewong; Korean J Parasitol 2013;51(6):645-650.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.645

Abstract
PDF

A simple, rapid, and high-throughput method for detection and identification of Wuchereria bancrofti, Brugia malayi, Brugia pahangi, and Dirofilaria immitis in mosquito vectors and blood samples was developed using a real-time PCR combined with high-resolution melting (HRM) analysis. Amplicons of the 4 filarial species were generated from 5S rRNA and spliced leader sequences by the real-time PCR and their melting temperatures were determined by the HRM method. Melting of amplicons from W. bancrofti, B. malayi, D. immitis, and B. pahangi peaked at 81.5±0.2℃, 79.0±0.3℃, 76.8±0.1℃, and 79.9±0.1℃, respectively. This assay is relatively cheap since it does not require synthesis of hybridization probes. Its sensitivity and specificity were 100%. It is a rapid and technically simple approach, and an important tool for population surveys as well as molecular xenomonitoring of parasites in vectors.

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