Fasciola hepatica is a species of zoonotic liver flukes with a broad range of definitive hosts worldwide. However, this liver fluke has not been detected in Korean water deer (Hydropotes inermis argyropus). This study provides the first evidence for Korean water deer being a definitive host of F. hepatica.
Culicoides biting midges (Diptera: Ceratopogonidae) are hematophagous arthropod vectors that transmit epizootic arthropod-borne viruses (arboviruses). Arboviruses are recognized as causes of pregnancy loss, encephalomyelitis, and congenital malformations in ruminants. Therefore, continuous monitoring and control of Culicoides, which causes significant damage to industrial animals are necessary. We performed attraction and repellent tests in Culicoides using various essential oils, cow dung, and carbon dioxide (CO2). Culicoides tended to move more to cow dung (60.8%, P<0.0001) and CO2 (63.8%, P<0.01). To the essential oils as repellents, 26.1% (P<0.0001), 18.7% (P<0.001), and 25.5% (P<0.01) of the Culicoides moved to the lavender, lemongrass, and eucalyptus chamber, respectively. The Culicoides that moved to the 3 essential oils chambers showed markedly low activity. Collectively, it was showed that Culicoides tended to be attractive to cow dung and CO2, and repellent from the 3 essential oils.
Present study was performed to describe the morphological and molecular characterization of Toxocara tanuki (Nematoda: Ascaridae) from Korean raccoon dog, Nyctereutes procyonoides koreensis, naturally infected in the Republic of Korea (Korea). Juvenile and adult worms of T. tanuki were recovered in 5 out of 10 raccoon dogs examined and the larval worms were detected in 15 out of 20 muscle samples (75%). Small lateral alae were observed on the cranial end of the body in male and female adults and 2 long spicules (3.0-3.5 mm) were characteristically observed in the posterior end of males. In SEM observation, 18 pairs of proximal precloacal, a precloacal median, a postcloacal median and 5 pairs of postcloacal papillae were uniquely revealed in the posterior portion of males, but the proximal papillae were not shown in the lateral ends of females. Molecular analysis on the 18S rRNA partial DNA sequences was revealed the same finding in both samples, adult worms and muscle larvae, which are closely related to T. tanuki. In conclusion, it was confirmed for the first time that T. tanuki is indigenously distributed, the Korean raccoon dog is acted as the natural definitive host of this nematode in Korea and the morphological characteristics of T. tanuki were shown in specific structure for single postcloacal median papilla in male.
Citations
Citations to this article as recorded by
Metabarcoding of pathogenic parasites based on copro-DNA analysis of wild animals in South Korea Jun Ho Choi, Soo Lim Kim, Dong Kyun Yoo, Myung-hee Yi, Singeun Oh, Myungjun Kim, Sohyeon Yun, Tai-Soon Yong, Seongjun Choe, Jong Koo Lee, Ju Yeong Kim Heliyon.2024; 10(9): e30059. CrossRef
Toxocara tanuki larval distribution in mice and the infectivity of tissue larvae Natsuki Okada, Hong-Kean Ooi, Kensuke Taira Parasitology Research.2023; 122(6): 1327. CrossRef
Detection of larvae of Toxocara cati and T. tanuki from the muscles of free-ranging layer farm chickens Natsuki Okada, Hong-Kean Ooi, Kensuke Taira Parasitology Research.2021; 120(5): 1737. CrossRef
Morphological and Molecular Characterization of Toxocara apodemi (Nematoda: Ascarididae) from Striped Field Mice, Apodemus agrarius, in Korea Hyeon Cheol Kim, Eui Ju Hong, Si Yun Ryu, Jinho Park, Jeong Gon Cho, Do Hyeon Yu, Joon Seok Chae, Kyoung Seong Choi, Bae Keun Park The Korean Journal of Parasitology.2020; 58(4): 403. CrossRef
Arthropod-borne viruses (Arboviruses) are transmitted by arthropods such as Culicoides biting midges and cause abortion, stillbirth, and congenital malformation in ruminants, apparently leading to economic losses to farmers. To monitor the distribution of Culicoides and to determine their relationship with different environmental conditions (temperature, humidity, wind speed, and altitude of the farms) on 5 cattle farms, Culicoides were collected during summer season (May-September) in 2016 and 2017, and analyzed for identification of species and detection of arboviruses. About 35% of the Culicoides were collected in July and the collection rate increased with increase in temperature and humidity. The higher altitude where the farms were located, the more Culicoides were collected on inside than outside. In antigen test of Culicoides against 5 arboviruses, only Chuzan virus (CHUV) (2.63%) was detected in 2016. The Akabane virus (AKAV), CHUV, Ibaraki virus and Bovine ephemeral fever virus (BEFV) had a positive rate of less than 1.8% in 2017. In antigen test of bovine whole blood, AKAV (12.96%) and BEFV (0.96%) were positive in only one of the farms. As a result of serum neutralization test, antibodies against AKAV were generally measured in all the farms. These results suggest that vaccination before the season in which the Culicoides are active is probably best to prevent arbovirus infections.
Citations
Citations to this article as recorded by
Isolation and identification of an AKAV strain in dairy cattle in China Miaomiao Zhang, Menghua Deng, Sisi Zhao, Dengshuai Zhao, Yajie Zheng, Limei Qin, Han Gao, Mengmeng Zhao, Keshan Zhang Frontiers in Veterinary Science.2025;[Epub] CrossRef
The Role of Hematophagous Arthropods, Other than Mosquitoes and Ticks, in Arbovirus Transmission Bradley J. Blitvich Viruses.2025; 17(7): 932. CrossRef
Coinfection of Bovine ephemeral fever virus and ‘Candidatus Mycoplasma haemobos’ in cattle in Central China: implications for tick-borne transmission and viral divergence Hongfei Shi, Tingting Lv, Zhengtian Zhang, Hongyue Zhai, Li Wang, Shidong Ji, Dandan Li, Lunguang Yao BMC Veterinary Research.2025;[Epub] CrossRef
Hiding in plain sight: Uncovering the hidden diversity of Culicoides spp. (Diptera: Ceratopogonidae) in the Republic of Korea using DNA barcoding data Jiseung Jeon, Dong-Yeol Lee, Seung Bak An, Jihun Ryu, Jong-Uk Jeong, In-Soon Roh, Kwang Shik Choi Acta Tropica.2025; 270: 107821. CrossRef
Bovine ephemeral fever: From underestimated illness to emerging threat - A review of pathogenesis, economic impact, and future control strategies Shruti Pyasi, Neha Rani Sahu, Umasish Mohanty, Debasis Nayak Research in Veterinary Science.2025; 196: 105904. CrossRef
Research progress on the N gene of Akabane virus Ruining Wang, Fang Liang, Xiaolin Lan, Gan Li, Feng Li, Mengmeng Zhao Frontiers in Veterinary Science.2025;[Epub] CrossRef
Potential roles of Culicoides spp. (Culicoides imicola, Culicoides oxystoma) as biological vectors of bluetongue virus in Yuanyang of Yunnan, P. R. China Nan Li, Jinxin Meng, Yuwen He, Wenhua Wang, Jinglin Wang Frontiers in Cellular and Infection Microbiology.2024;[Epub] CrossRef
Role of cervids in the epidemiology of bovine ephemeral fever virus infection in the Republic of Korea: A cross‐sectional retrospective study Jung‐Yong Yeh, Yun Ji Ga Veterinary Medicine and Science.2023; 9(1): 301. CrossRef
Composition and diversity of Culicoides biting midges (Diptera: Ceratopogonidae) in rural and suburban environments of Algeria Nadia Kadjoudj, Azzedine Bounamous, Yacine Kouba, Bilal Dik, Samir Zeroual, Aicha Amira, Haroun Chenchouni Acta Tropica.2022; 234: 106588. CrossRef
Genetically modified rabies virus vector‐based bovine ephemeral fever virus vaccine induces protective immune responses against BEFV and RABV in mice Wenwen Zheng, Zhongxin Zhao, Li Tian, Lele Liu, Tong Xu, Xianwei Wang, Hongbin He, Xianzhu Xia, Ye Zheng, Yurong Wei, Xuexing Zheng Transboundary and Emerging Diseases.2021; 68(3): 1353. CrossRef
Detection of arboviruses in Culicoides (Diptera: Ceratopogonidae) collected from animal farms in the border areas of Yunnan Province, China Di DI, Chen-xi LI, Zong-jie LI, Xin WANG, Qi-qi XIA, Mona SHARMA, Bei-bei LI, Ke LIU, Dong-hua SHAO, Ya-feng QIU, WAI Soe-Soe, Shi-biao YANG, Jian-chao WEI, Zhi-yong MA Journal of Integrative Agriculture.2021; 20(9): 2491. CrossRef
Attraction and Repellent Behaviors of Culicoides Biting Midges toward Cow Dung, Carbon Dioxide, and Essential Oils Daram Yang, Myeon-Sik Yang, Bumseok Kim The Korean Journal of Parasitology.2021; 59(5): 465. CrossRef
The Ecology and Evolution of Japanese Encephalitis Virus Peter Mulvey, Veasna Duong, Sebastien Boyer, Graham Burgess, David T. Williams, Philippe Dussart, Paul F. Horwood Pathogens.2021; 10(12): 1534. CrossRef
Investigation of bovine ephemeral fever virus transmission by putative dipteran vectors under experimental conditions Jessica E. Stokes, Karin E. Darpel, Simon Gubbins, Simon Carpenter, María del Mar Fernández de Marco, Luis M. Hernández-Triana, Anthony R. Fooks, Nicholas Johnson, Christopher Sanders Parasites & Vectors.2020;[Epub] CrossRef
Bovine Ephemeral Fever in Asia: Recent Status and Research Gaps Fan Lee Viruses.2019; 11(5): 412. CrossRef
First
Prev
