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"Chenghua Shen"

Original Article

Serodiagnosis of Toxocariasis by ELISA Using Crude Antigen of Toxocara canis Larvae
Yan Jin, Chenghua Shen, Sun Huh, Woon-Mok Sohn, Min-Ho Choi, Sung-Tae Hong
Korean J Parasitol 2013;51(4):433-439.
Published online August 30, 2013
DOI: https://doi.org/10.3347/kjp.2013.51.4.433

Toxocariasis is a worldwide zoonosis caused by larvae of ascarid nematodes of dogs or cats, Toxocara canis or T. cati. Diagnosis of human toxocariasis currently relies on serology that uses T. canis excretory-secretory antigen to detect specific IgG antibodies by ELISA. We investigated the serodiagnostic efficacy of ELISA using crude antigen of T. canis larvae (TCLA). Serum specimens of 64 clinically confirmed toxocariasis, 115 healthy controls, and 119 other tissue-invading helminthiases were screened by ELISA using TCLA. The ELISA using TCLA showed 92.2% (59/64 patient samples) sensitivity and 86.6% (103/119) specificity. Its positive diagnostic predictivity was 78.7% and negative predictivity was 97.8%. No serum of healthy controls reacted but that of anisakiasis (45.5%), gnathostomiasis (19.2%), clonorchiasis (15.8%), sparganosis (11.1%), and cysticercosis (6.3%) cross-reacted. Immunoblot analysis on TCLA recognized antigenic proteins of 28- and 30-kDa bands in their dominant protein quantity and strong blotting reactivity. The present results indicate that the ELISA using our TCLA antigen is acceptable by the sensitivity and specificity for serodiagnosis of human toxocariasis. ELISA with TCLA is recommended to make differential diagnosis for patients with any sign of organ infiltration and eosinophilia.

Citations

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  • Epidemiological profile of human toxocariasis in patients examined at Evandro Chagas Institute (IEC/SVSA/MS) between 2014 and 2019
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    Comparative Immunology, Microbiology and Infectious Diseases.2024; 105: 102112.     CrossRef
  • Evaluation of Somatic Antigens of Adult Toxocara helminthes for Detection of Human Toxocariasis
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    Parasite Immunology.2024;[Epub]     CrossRef
  • Toxocara canis and Fasciola hepatica Co-Infection Leading to Hepatic Abscess: A Case Report
    Seung Wan Kim, Byoung Kuk Jang
    Journal of Korean Medical Science.2023;[Epub]     CrossRef
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  • A Neurotoxocariasis Case Manifesting Multiple Cerebral Infarction and Eosinophilic Meningoencephalitis
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  • Prevalence of Toxocariasis and Its Risk Factors in Patients with Eosinophilia in Korea
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    The Korean Journal of Parasitology.2020; 58(4): 413.     CrossRef
  • An innovative approach in the detection of Toxocara canis excretory/secretory antigens using specific nanobodies
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    International Journal for Parasitology.2019; 49(8): 635.     CrossRef
  • Human toxocariasis – A look at a neglected disease through an epidemiological ‘prism’
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  • Ocular toxocariasis: a neglected parasitic disease in Egypt
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  • Clinical Profile, Treatment, and Visual Outcome of Ocular Toxocara in a Tertiary Eye Care Centre
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    Clinical & Experimental Allergy.2018; 48(1): 66.     CrossRef
  • Toxocariasis: a silent threat with a progressive public health impact
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  • Detection of Toxocara canis DNA in tissues of experimentally infected mice
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    Journal of Korean Medical Science.2018;[Epub]     CrossRef
  • Genetic analysis of Toxocara spp. in stray cats and dogs in Van province, Eastern Turkey
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  • Proteomic analysis of Toxocara canis excretory and secretory (TES) proteins
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  • Clinical usefulness of Western blotting and ELISA avidity for the diagnosis of human toxocariasis
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  • The Clinical Characteristics of Ocular Toxocariasis in Jeju Island Using Ultra-wide-field Fundus Photography
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  • Association between intermediate uveitis and toxocariasis in the Korean population
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  • Humoral response of mice infected with Toxocara canis following different infection schemes
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  • Sero-detection of Toxocara canis infection in human with T.canis recombinant arginine kinase, cathepsin L-1 and TES-26 antigens
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Brief Communication

Collection of Clonorchis sinensis adult worms from infected humans after praziquantel treatment
Chenghua Shen, Jae-hwan Kim, Jeong-Keun Lee, Young Mee Bae, Min-Ho Choi, Jin-Kyoung Oh, Min Kyung Lim, Hai-Rim Shin, Sung-Tae Hong
Korean J Parasitol 2007;45(2):149-152.
Published online June 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.2.149

A cohort was established for evaluation of cancer risk factors in Sancheong-gun, Gyeongsangnam-do, Korea. As one of the cohort studies, stools of 947 residents (403 males and 544 females, age range: 29-86 years) were screened for Clonorchis sinensis eggs using both Kato-Katz method and formalin-ether sedimentation technique. The overall egg positive rate of C. sinensis was 37.7% and individual EPG (eggs per gram of feces) counts ranged from 24 to 28,800. Eight egg positive residents voluntarily joined a process of collection of the passed worms after praziquantel treatment. A total of 158 worms were recovered from 5 of the 8 treated persons, ranged from 3 to 108 in each individual. The worms were 15-20 mm × 2-3 mm in size, and showed brown-pigmented, red, or white body colors. This is the first collection record of C. sinensis adult worms from humans through anthelmintic treatment and purgation. The adult worms of C. sinensis may be paralyzed by praziquantel and then discharged passively through bile flow in the bile duct and by peristaltic movement of the bowel.

Citations

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  • Clonorchis sinensis and Cholangiocarcinoma
    Eun-Min Kim, Sung-Tae Hong
    Journal of Korean Medical Science.2025;[Epub]     CrossRef
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    Thomas Crellen, Francesca Vita, Chiara Braconi, Paiboon Sithithaworn, T. Déirdre Hollingsworth
    Proceedings of the National Academy of Sciences.2025;[Epub]     CrossRef
  • Diagnosis of helminths depends on worm fecundity and the distribution of parasites within hosts
    Thomas Crellen, Melissa Haswell, Paiboon Sithithaworn, Somphou Sayasone, Peter Odermatt, Poppy H. L. Lamberton, Simon E. F. Spencer, T. Déirdre Hollingsworth
    Proceedings of the Royal Society B: Biological Sciences.2023;[Epub]     CrossRef
  • A comparison of helminth infections as assessed through coprological analysis and adult worm burdens in a wild host
    Rachel L. Byrne, Ursula Fogarty, Andrew Mooney, Nicola M. Marples, Celia V. Holland
    International Journal for Parasitology: Parasites and Wildlife.2018; 7(3): 439.     CrossRef
  • Application of a loop-mediated isothermal amplification (LAMP) assay targeting cox1 gene for the detection of Clonorchis sinensis in human fecal samples
    S. M. Mazidur Rahman, Hyun Beom Song, Yan Jin, Jin-Kyoung Oh, Min Kyung Lim, Sung-Tae Hong, Min-Ho Choi, Justin V. Remais
    PLOS Neglected Tropical Diseases.2017; 11(10): e0005995.     CrossRef
  • Cohort Profile: Community-based prospective cohort from the National Cancer Center, Korea
    Jin-Kyoung Oh, Min Kyung Lim, E Hwa Yun, Min-Ho Choi, Sung-Tae Hong, Soung-Hoon Chang, Sue Kyung Park, Sung-Il Cho, Dong-Hyun Kim, Keun-Young Yoo, Hai-Rim Shin
    International Journal of Epidemiology.2015; : dyv302.     CrossRef
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    Eun Joo Chung, Tae Yun Kim, Sung-Jong Hong, Tai-Soon Yong
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    Sung-Tae Hong, Yueyi Fang
    Parasitology International.2012; 61(1): 17.     CrossRef
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    S.M. Mazidur Rahman, Min-Ho Choi, Young Mee Bae, Sung-Tae Hong
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  • Oriental Cholangiohepatitis (Clonorchiasis Infestation) Caused by Clonorchis Sinensis
    Sarah Klemencic, Michael Phelan, Robert Patrick, Noushin Vahdat
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  • Early detection and estimation of infection burden by real-time PCR in rats experimentally infected with Clonorchis sinensis
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  • Hypersensitive Reaction to Praziquantel in a Clonorchiasis Patient
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    Jun Uk Lim, Kwang Ro Joo, Hyun Phil Shin, Jae Myung Cha, Joung Il Lee, Sung Jig Lim
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  • 13,363 View
  • 94 Download
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Original Article

Tissue parasitic helminthiases are prevalent at Cheongjin, North Korea
Chenghua Shen, Shunyu Li, Shanzi Zheng, Min-Ho Choi, Young Mee Bae, Sung-Tae Hong
Korean J Parasitol 2007;45(2):139-144.
Published online June 20, 2007
DOI: https://doi.org/10.3347/kjp.2007.45.2.139

We investigated a small-scale serological survey to screen tissue-parasitic helminthiases of North Koreans as one of research programs for re-unification of Korea. Soil-transmitted helminthiases were found highly prevalent among North Korean residents at the border with China. ELISA using 4 tissue-parasitic helminth antigens was applied to 137 residents living in Cheongjin-shi, Hamgyeongbuk-do, North Korea and 133 female refugees in South Korea in 2004-2005. Among a total of 270 samples, 31 (11.5%), 25 (9.3%), and 11 (4.1%) were positive for specific IgG antibodies to antigens of Clonorchis sinensis, Taenia solium metacestode, and sparganum, respectively. The overall positive rate was 21.5%; 38.2% in males and 15.8% in females. The present finding suggests that tissue parasites, such as C. sinensis, T. solium metacestode and sparganum are highly prevalent in some limited areas of North Korea. These foodborne tissue-parasitic helminthiases should be considered for future control measures of parasitic diseases in North Korea.

Citations

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  • Relationship between Clonorchis sinensis Infection and Cholangiocarcinoma in Korea
    Hwa Sun Kim, Ho-Woo Nam, Hye-Jin Ahn, Dongjae Kim, Yeong Hoon Kim
    The Korean Journal of Parasitology.2022; 60(4): 261.     CrossRef
  • Taeniasis and cysticercosis in Asia: A review with emphasis on molecular approaches and local lifestyles
    Akira Ito, Tiaoying Li, Toni Wandra, Paron Dekumyoy, Tetsuya Yanagida, Munehiro Okamoto, Christine M Budke
    Acta Tropica.2019; 198: 105075.     CrossRef
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    Eunsuk Chang
    Pathogens and Global Health.2019; 113(6): 256.     CrossRef
  • Infections of Soil-Transmitted Helminth in Refugees from North Korea
    Young-Il Lee, Min Seo, Suk-Bae Kim
    The Korean Journal of Parasitology.2018; 56(3): 291.     CrossRef
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    Uffe Christian Braae, Nguyen Manh Hung, Fadjar Satrija, Virak Khieu, Xiao-Nong Zhou, Arve Lee Willingham
    Parasites & Vectors.2018;[Epub]     CrossRef
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    Jong-Yil Chai
    The Korean Journal of Parasitology.2013; 51(1): 9.     CrossRef
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    Men-Bao Qian, Ying-Dan Chen, Song Liang, Guo-Jing Yang, Xiao-Nong Zhou
    Infectious Diseases of Poverty.2012;[Epub]     CrossRef
  • 8,873 View
  • 67 Download
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Brief Communication
Status of intestinal helminthic infections of borderline residents in North Korea
Shunyu Li, Chenghua Shen, Min-Ho Choi, Young Mee Bae, Hiwon Yoon, Sung-Tae Hong
Korean J Parasitol 2006;44(3):265-268.
Published online September 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.3.265

The present authors investigated intestinal parasitic infections among North Korean residents and refugees in China in 2003. The Kato-Katz method was applied to 236 residents and soldiers in a town on the North Korea-China border and to 46 people at a refugee camp in China. Only eggs of Ascaris and Trichuris were detected, with egg positive rates of 41.1% and 37.6%, respectively. The total egg positive rate was 55.0% and most of those who were egg positive were only lightly infected. Women of 61.2% and men of 53.1% were egg positive. The refugees from rural areas showed higher egg positive rates than those from urban areas. The present investigation confirmed high prevalence of soil-transmitted intestinal helminths in rural borderline areas of North Korea.

Citations

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  • Helminthiases in North Korea: a neglected public health challenge
    Eunsuk Chang
    Pathogens and Global Health.2019; 113(6): 256.     CrossRef
  • Helminthiasis among North Koreans: a chronic public health challenge.
    Kee B. Park, Nagi M. Shafik
    Pathogens and Global Health.2019; 113(6): 255.     CrossRef
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    John J Park, Ah-Young Lim, Hyung-Soon Ahn, Andrew I Kim, Soyoung Choi, David HW Oh, Owen Lee-Park, Sharon Y Kim, Sun Jae Jung, Jesse B Bump, Rifat Atun, Hee Young Shin, Kee B Park
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    Do-Hyeon Park, Min-Ho Choi, Ah-Young Lim, Hee Young Shin
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    Sung-Tae Hong
    Journal of Korean Medical Science.2018;[Epub]     CrossRef
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    Young-Il Lee, Min Seo, Suk-Bae Kim
    The Korean Journal of Parasitology.2018; 56(3): 291.     CrossRef
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    Sun Wook Cho, So Hee Lee, Eun Sil Koh, Si Eun Kim, Seok Joong Kim
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    Eugene S. Yim, Ricky Y. Choi, Michael J. VanRooyen
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  • Tissue parasitic helminthiases are prevalent at Cheongjin, North Korea
    Chenghua Shen, Shunyu Li, Shanzi Zheng, Min-Ho Choi, Young Mee Bae, Sung-Tae Hong
    The Korean Journal of Parasitology.2007; 45(2): 139.     CrossRef
  • Private Wells and Disaster Preparedness
    N. Fuke
    Prehospital and Disaster Medicine.2005; 20(S2): S142.     CrossRef
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