Cystic echinococcosis (CE), a zoonotic disease caused by Echinococcusgranulosus at the larval stage, predominantly develops in the liver and lungs of intermediate hosts and eventually results in organ malfunction or even death. The interaction between E. granulosus and human body is incompletely understood. Exosomes are nanosized particles ubiquitously present in human body fluids. Exosomes carry biomolecules that facilitate communication between cells. To the best of our knowledge, the role of exosomes in patients with CE is not reported. Here, we isolated exosomes from the sera of patients with CE (CE-exo) and healthy donors and subjected them to liquid chromatography-tandem mass spectrometry analysis. Proteomic analysis identified 49 proteins specifically expressed in CE-exo, including 4 proteins of parasitic origin. The most valuable parasitic proteins included tubulin alpha-1C chain and histone H4. And 8 proteins were differentially regulated in CE-exo (fold change>1.5), as analyzed with bioinformatic methods such as annotation and functional enrichment analyses. These findings may improve our understanding about the interaction between E. granulosus and human body, and may contribute to the diagnosis and prevention of CE.
Citations
Citations to this article as recorded by
Progresses and challenges in
Strongyloides
spp. proteomics
Natalia Tiberti, Marcello Manfredi, Chiara Piubelli, Dora Buonfrate Philosophical Transactions of the Royal Society B: Biological Sciences.2024;[Epub] CrossRef
A pilot study of microRNAs expression profile in plasma of patients with hydatid disease: potential immunomodulation of hydatid disease Jing Xiao, Jian-Wen Wu, Yun-Zhuo Xin, Dong Song, Xiao-ping Gao, Mei Yin, Wei Zhao, Fu-lin Liu, Hao Wang, Jie Wang, Jia-Qing Zhao Parasitology Research.2024;[Epub] CrossRef
Characterization of protein cargo of Echinococcus granulosus extracellular vesicles in drug response and its influence on immune response María Celeste Nicolao, Christian Rodriguez Rodrigues, Magalí B. Coccimiglio, Camila Ledo, Guillermo H. Docena, Andrea C. Cumino Parasites & Vectors.2023;[Epub] CrossRef
Proteomic Analysis of Plasma-Derived Extracellular Vesicles From Mice With Echinococcus granulosus at Different Infection Stages and Their Immunomodulatory Functions Chunli Shi, Xiaojing Zhou, Wenjuan Yang, Jianwen Wu, Min Bai, Ying Zhang, Wei Zhao, Hui Yang, Atsushi Nagai, Mei Yin, Xiaoping Gao, Shuqin Ding, Jiaqing Zhao Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Expression profiling of exosomal miRNAs derived from different stages of infection in mice infected with Echinococcus granulosus protoscoleces using high-throughput sequencing Jing Xiao, Yazhou Zhu, Jianwen Wu, Min Bai, Yunzhuo Xin, Qiang Wang, Jiaqing Zhao Parasitology Research.2022; 121(7): 1993. CrossRef
Characterization of the B-Cell Epitopes of Echinococcus granulosus Histones H4 and H2A Recognized by Sera From Patients With Liver Cysts Andrea Maglioco, Facundo A. Agüero, María Pía Valacco, Alejandra Juárez Valdez, Margot Paulino, Alicia G. Fuchs Frontiers in Cellular and Infection Microbiology.2022;[Epub] CrossRef
Extracellular vesicles secreted by model tapeworm Hymenolepis diminuta: biogenesis, ultrastructure and protein composition Hynek Mazanec, Peter Koník, Zdenko Gardian, Roman Kuchta International Journal for Parasitology.2021; 51(5): 327. CrossRef
Identification of Different Extracellular Vesicles in the Hydatid Fluid of Echinococcus granulosus and Immunomodulatory Effects of 110 K EVs on Sheep PBMCs Jing Yang, Jin'en Wu, Yong Fu, Lujun Yan, Yating Li, Xiaola Guo, Yong'e Zhang, Xiaoqiang Wang, Yujuan Shen, William C. Cho, Yadong Zheng Frontiers in Immunology.2021;[Epub] CrossRef
Extensive mitochondrial proteome disturbance occurs during the early stages of acute myocardial ischemia Jie Wang, Jun He, Yucheng Fan, Fangjing Xu, Qian Liu, Ruhua He, Ru Yan Experimental and Therapeutic Medicine.2021;[Epub] CrossRef
Characterization of exosome-like vesicles derived from Taenia pisiformis cysticercus and their immunoregulatory role on macrophages Li-Qun Wang, Ting-Li Liu, Pan-Hong Liang, Shao-Hua Zhang, Tao-Shan Li, Yan-Ping Li, Guang-Xue Liu, Li Mao, Xue-Nong Luo Parasites & Vectors.2020;[Epub] CrossRef
First
Prev
