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"Da-Won Ma"

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Outbreak of Cyclosporiasis in Korean Travelers Returning from Nepal
Da-Won Ma, Myoung-Ro Lee, Bora Ku, Shin-Hyeong Cho, Sang-Eun Lee
Korean J Parasitol 2020;58(5):589-592.
Published online October 22, 2020
DOI: https://doi.org/10.3347/kjp.2020.58.5.589
Cyclospora cayetanensis is an apicomplexan protozoan and is one of the most common pathogens causing chronic diarrhea worldwide. Eight stool samples with diarrheal symptom out of 18 Korean residents who traveled to Nepal were obtained, and examined for 25 enteropathogens including 16 bacterial species, 5 viral species, and 4 protozoans in stool samples as causative agents of water-borne and food-borne disease. Only C. cayetanensis was detected by nested PCR, and 3 PCR-positive samples were sequenced to confirm species identification. However, the oocysts of C. cayetanensis in fecal samples could not be detected by direct microscopy of the stained sample. As far as we know, this is the first report of a group infection with C. cayetanensis from a traveler visiting Nepal, and the second report of a traveler’s diarrhea by C. cayetanensis imported in Korea.

Citations

Citations to this article as recorded by  Crossref logo
  • Food Safety in Developing Countries: Common Foodborne and Waterborne Illnesses, Regulations, Organizational Structure, and Challenges of Food Safety in the Context of Nepal
    Deepak Subedi, Madhav Paudel, Sandesh Poudel, Niranjan Koirala
    Food Frontiers.2025; 6(1): 86.     CrossRef
  • Imported parasitic diseases in the Republic of Korea: status and issues
    Jong-Yil Chai
    Journal of the Korean Medical Association.2025; 68(1): 52.     CrossRef
  • Cyclospora and Cyclosporiasis: The Nepalese Perspective
    Pitambar Dhakal, Junqiang Li, Bishnu Maya K.C., Yin Fu, Yuancai Chen, Longxian Zhang
    Zoonoses.2024;[Epub]     CrossRef
  • Cyclospora cayetanensis: A Perspective (2020–2023) with Emphasis on Epidemiology and Detection Methods
    Sonia Almeria, Leonor Chacin-Bonilla, Jenny G. Maloney, Monica Santin
    Microorganisms.2023; 11(9): 2171.     CrossRef
  • Waterborne transmission of protozoan parasites: a review of worldwide outbreaks – an update 2017–2022
    Pavlina Bourli, Aida Vafae Eslahi, Ourania Tzoraki, Panagiotis Karanis
    Journal of Water and Health.2023; 21(10): 1421.     CrossRef
  • Waterborne protozoan outbreaks: An update on the global, regional, and national prevalence from 2017 to 2020 and sources of contamination
    Jun-Yang Ma, Man-Yao Li, Ze-Zheng Qi, Ming Fu, Tian-Feng Sun, Hany M. Elsheikha, Wei Cong
    Science of The Total Environment.2022; 806: 150562.     CrossRef
  • Review on Cyclosporiasis Outbreaks and Potential Molecular Markers for Tracing Back Investigations
    Junqiang Li, Feifei Xu, Md Robiul Karim, Longxian Zhang
    Foodborne Pathogens and Disease.2022; 19(12): 796.     CrossRef
  • 4,745 View
  • 99 Download
  • 7 Web of Science
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Molecular Prevalence and Genotypes of Cryptosporidium parvum and Giardia duodenalis in Patients with Acute Diarrhea in Korea, 2013-2016
Da-Won Ma, Myoung-Ro Lee, Sung-Hee Hong, Shin-Hyeong Cho, Sang-Eun Lee
Korean J Parasitol 2019;57(5):531-536.
Published online October 31, 2019
DOI: https://doi.org/10.3347/kjp.2019.57.5.531
Cryptosporidium parvum and Giardia duodenalis are the main diarrhea-causing parasitic pathogens; however, their prevalence in Korea is unknown. Here, we conducted a survey to determine the prevalence and genotype distribution of these 2 pathogens causing acute diarrhea in 8,571 patients hospitalized in 17 Regional Institute of Health Environment sites in Korea, during 2013-2016. C. parvum and G. duodenalis were detected and genotyped by nested PCR, and the isolate were molecularly characterized by sequencing the glycoprotein 60 (Gp60) and β-giardin genes, respectively. The overall prevalence of C. parvum and G. duodenalis was 0.37% (n=32) and 0.55% (n=47), respectively, and both pathogens were more prevalent in children under 9 years old. Molecular epidemiological analysis showed that the C. parvum isolates belonged to the IIa family and were subtyped as IIaA13G2R1, IIaA14G2R1, IIaA15G2R1, and IIaA18G3R1. Analysis of the β-giardin gene fragment from G. duodenalis showed that all positive strains belong to assemblage A. This is the first report on the molecular epidemiology and subtyping of C. parvum and G. duodenalis in such a large number of diarrheal patients in Korea. These results highlight the need for continuous monitoring of these zoonotic pathogens and provide a basis for implementing control and prevention strategies. Further, the results might be useful for epidemiological investigation of the source of outbreak.

Citations

Citations to this article as recorded by  Crossref logo
  • An Epidemiology Study on Distribution of Giardia lamblia in Incheon City
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    Franziska Göhring, Matthias Lendner, Arwid Daugschies
    Veterinary Parasitology: Regional Studies and Reports.2022; 36: 100806.     CrossRef
  • Annual Report on the External Quality Assessment Scheme for Clinical Parasitology in Korea (2020–2021)
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    Veterinární medicína.2022; 67(3): 156.     CrossRef
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    Eell Ryoo
    Clinical and Experimental Pediatrics.2021; 64(6): 260.     CrossRef
  • Clinical and Laboratory Diagnosis of Cryptosporidiosis among Children with Acute Gastroenteritis at a Tertiary Hospital, Cairo, Egypt
    Mohammad Ashraf Abdel Wahed, Yasmine Elsayed Abdelrahman Shehab, Hanan Mahmoud Abou-Seri, Yosra Mohamed Mohsen Awad
    Journal of Tropical Pediatrics.2021;[Epub]     CrossRef
  • Giardia duodenalis Virulence — “To Be, or Not To Be”
    Raúl Argüello-García, M. Guadalupe Ortega-Pierres
    Current Tropical Medicine Reports.2021; 8(4): 246.     CrossRef
  • Cross-Border Investigations on the Prevalence and Transmission Dynamics of Cryptosporidium Species in Dairy Cattle Farms in Western Mainland Europe
    Pedro Pinto, Cláudia A. Ribeiro, Sumaiya Hoque, Ourida Hammouma, Hélène Leruste, Sébastien Détriché, Evi Canniere, Yvonne Daandels, Martine Dellevoet, Janine Roemen, Anne Barbier Bourgeois, Martin Kváč, Jérôme Follet, Anastasios D. Tsaousis
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  • Asymptomatic Cryptosporidium infections in ewes and lambs are a source of environmental contamination with zoonotic genotypes of Cryptosporidium parvum
    Léa Bordes, Pauline Houert, Damien Costa, Loïc Favennec, Corinne Vial-Novella, Francis Fidelle, Christelle Grisez, Françoise Prévot, Philippe Jacquiet, Romy Razakandrainibe
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    Suhak Jeon, Eun Jeong Won, Yu Jeong Lee, Moon-Ju Kim, Myung Geun Shin, Jong Hee Shin
    Journal of Laboratory Medicine and Quality Assurance.2020; 42(4): 177.     CrossRef
  • 8,243 View
  • 129 Download
  • 14 Web of Science
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Original Articles
Development of Molecular Diagnosis Using Multiplex Real-Time PCR and T4 Phage Internal Control to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis from Human Stool Samples
Ji-Hun Shin, Sang-Eun Lee, Tong Soo Kim, Da-Won Ma, Shin-Hyeong Cho, Jong-Yil Chai, Eun-Hee Shin
Korean J Parasitol 2018;56(5):419-427.
Published online October 31, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.5.419
This study aimed to develop a new multiplex real-time PCR detection method for 3 species of waterborne protozoan parasites (Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis) identified as major causes of traveler’s diarrhea. Three target genes were specifically and simultaneously detected by the TaqMan probe method for multiple parasitic infection cases, including Cryptosporidium oocyst wall protein for C. parvum, glutamate dehydrogenase for G. lamblia, and internal transcribed spacer 1 for C. cayetanensis. Gene product 21 for bacteriophage T4 was used as an internal control DNA target for monitoring human stool DNA amplification. TaqMan probes were prepared using 4 fluorescent dyes, FAMTM, HEXTM, Cy5TM, and CAL Fluor Red® 610 on C. parvum, G. lamblia, C. cayetanensis, and bacteriophage T4, respectively. We developed a novel primer-probe set for each parasite, a primer-probe cocktail (a mixture of primers and probes for the parasites and the internal control) for multiplex real-time PCR analysis, and a protocol for this detection method. Multiplex real-time PCR with the primer-probe cocktail successfully and specifically detected the target genes of C. parvum, G. lamblia, and C. cayetanensis in the mixed spiked human stool sample. The limit of detection for our assay was 2×10 copies for C. parvum and for C. cayetanensis, while it was 2×103 copies for G. lamblia. We propose that the multiplex real-time PCR detection method developed here is a useful method for simultaneously diagnosing the most common causative protozoa in traveler’s diarrhea.

Citations

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    Biotechnology Letters.2025;[Epub]     CrossRef
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  • 19 Web of Science
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Multiplex-Touchdown PCR to Simultaneously Detect Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the Major Causes of Traveler’s Diarrhea
Ji-Hun Shin, Sang-Eun Lee, Tong Soo Kim, Da-Won Ma, Jong-Yil Chai, Eun-Hee Shin
Korean J Parasitol 2016;54(5):631-636.
Published online October 31, 2016
DOI: https://doi.org/10.3347/kjp.2016.54.5.631
This study aimed to develop a multiplex-touchdown PCR method to simultaneously detect 3 species of protozoan parasites, i.e., Cryptosporidium parvum, Giardia lamblia, and Cyclospora cayetanensis, the major causes of traveler’s diarrhea and are resistant to standard antimicrobial treatments. The target genes included the Cryptosporidium oocyst wall protein for C. parvum, Glutamate dehydrogenase for G. lamblia, and 18S ribosomal RNA (18S rRNA) for C. cayetanensis. The sizes of the amplified fragments were 555, 188, and 400 bps, respectively. The multiplex-touchdown PCR protocol using a primer mixture simultaneously detected protozoa in human stools, and the amplified gene was detected in >1×103 oocysts for C. parvum, >1×104 cysts for G. lamblia, and >1 copy of the 18S rRNA gene for C. cayetanensis. Taken together, our protocol convincingly demonstrated the ability to simultaneously detect C. parvum, G. lamblia, and C. cayetanenesis in stool samples.

Citations

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  • A Multiplex RT-PCR for the Detection of Three Viruses and One Viroid Infecting Hemp
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  • 260 Download
  • 9 Web of Science
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