Parasites, Hosts and Diseases

"Dae-Yong Kim"

Brief Communications

Seroprevalence of antibodies to Neospora caninum in dogs and raccoon dogs in Korea: Jae-Hoon Kim, Min-Soo Kang, Byung-Chun Lee, Woo-Suk Hwang, Chang-Woo Lee, Byung-Jae So, J. P. Dubey, Dae-Yong Kim; Korean J Parasitol 2003;41(4):243-245.
Published online December 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.4.243

Abstract
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Neospora caninum is an important cause of abortion in cattle, and dogs are its only known definitive host. Its seroprevalence among domestic urban and rural dogs and feral raccoon dogs (Nyctereutes procyonoides koreensis) in Korea was studied by indirect fluorescent antibody test (IFAT) and by the neospora agglutination test (NAT), respectively. Antibodies to N. caninum were found in 8.3% of urban dogs and in 21.6% of dogs at dairy farms. Antibody titers ranged from 1:50 to 1:400. Antibodies to N. caninum were found in six (23%) of 26 raccoon dogs. However, the potential role of raccoon dogs as a source of horizontal transmission of bovine neosporosis needs further investigation. The results of this study suggest that there is a close relationship between N. caninum infection among dairy farm dogs and cattle in Korea. This study reports for the first time upon the seroprevalence of N. caninum infection in raccoon dogs in Korea.

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ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera: Hye-Jin Ahn, Sera Kim, Dae-Yong Kim, Ho-Woo Nam; Korean J Parasitol 2003;41(3):175-177.
Published online September 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.3.175

Abstract
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An ELISA was established to measure bovine IgG directed against the recombinant antigenic determinant of Nc-p43, a major surface antigen of Neospora caninum. In a previous study, two thirds of the C-terminal of the molecule was expressed as a 6 × His tagged protein (Ncp43P) for ELISA using 2/3 of the N-terminal of SAG1 from Toxoplasma gondii as a control (TgSAG1A). Among 852 cattle sera collected from stock farms scattered nation-wide, 103 sera (12.1%) were found to react with Ncp43P positively, but no positive reaction was observed with TgSAG1A. This study shows that Ncp43P could be available as an efficient antigen for the diagnosis of neosporosis in cattle. Furthermore, it together with TgSAG1A, could be useful for the differential diagnosis of N. caninum and T. gondii infections in other mammals.

Citations

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Use of ELISA based on NcSRS2 of Neospora caninumexpressed in Pichia pastoris for diagnosing neosporosis in sheep and dogs
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Comparison of proteome and antigenic proteome between two Neospora caninum isolates
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Original Articles

Determination of antigenic domain in GST fused major surface protein (Nc-p43) of Neospora caninum: Eui-Sun Son, Hye-Jin Ahn, Jae-Hoon Kim, Dae-Yong Kim, Ho-Woo Nam; Korean J Parasitol 2001;39(3):241-246.
Published online September 30, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.3.241

Abstract
PDF

The antigenic domain of the major surface protein (Nc-p43) of Neospora caninum was examined by polymerase chain reaction of its gene fragments and recombinant expression as GST fusion proteins. The fragments of Nc-p43 were as follow: a total open reading frame (OFR), T; OFR without signal sequence and C-terminal hydrophobic sequence, S; N-terminal 2/3 parts of S, A; C-terminal 2/3 parts, P; N-terminal 1/3 part, X; middle 1/3 part, Y; and C-terminal 1/3 part, Z, respectively. The DNA fragments were cloned into pGEX-4T vector. Recombinant plasmids transformed into Escherichia coli of BL21 pLysS (DE3) strain were induced to express GST or GST fused fragments of Nc-p43 such as 69 kDa protein for T, 66 kDa for S, 52 kDa for A, 53 kDa for P, and 40 kDa proteins for X, Y, and Z, respectively in SDS-PAGE. The Nc-p43 fragments of T, S, and P reacted with a bovine serum of neosporosis while those of A, X, Y, and Z together with GST did not in the western blot. These findings suggest that the antigenic domain of Nc-p43 of N. caninum may be localized in the C-terminal 2/3 parts. Together with A19 clone in SAG1 of Toxoplasma gondii (Nam et al., 1996), the P fragment of Nc-p43 could be used as efficient antigens to diagnose and differentiate those infections with both species.

Citations

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Molecular characterization of Neospora caninum major antigens NcSAG1 and NcSRS2
Soledad Echeverría, Federico Carrión, Martín Soñora, Andrés Cabrera, Carlos Robello
Royal Society Open Science.2025;[Epub] CrossRef
Expression ofNeospora caninumNcSRS2 surface protein inPichia pastorisand its application for serodiagnosis ofNeosporainfection
Amanda Fernandes Pinheiro, Sibele Borsuk, Maria Elisabeth Aires Berne, Luciano da Silva Pinto, Renato Andreotti, Talita Roos, Barbara Couto Rollof, Fábio Pereira Leivas Leite
Pathogens and Global Health.2013; 107(3): 116. CrossRef
Induction of Interferon-Gamma (IFN-γ) and T Helper 1 (Th1) Immune Response by Bitter Gourd Extract
Kazunori IKE, Yuko UCHIDA, Tomohiko NAKAMURA, Soichi IMAI
Journal of Veterinary Medical Science.2005; 67(5): 521. CrossRef
ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera
Hye-Jin Ahn, Sera Kim, Dae-Yong Kim, Ho-Woo Nam
The Korean Journal of Parasitology.2003; 41(3): 175. CrossRef

8,149 View
82 Download
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Detection of IgG antibody against Neospora caninum in cattle in Korea: Ji-Seon Bae, Dae-Yong Kim, Woo-Suk Hwang, Jae-Hoon Kim, Nam-Seok Lee, Ho-Woo Nam; Korean J Parasitol 2000;38(4):245-249.
Published online December 31, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.4.245

Abstract
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A total of 492 cattle sera was screened by IgG-ELISA against Neospora caninum (Nc-1 strain and a Korean isolate, KBA-2) and Toxoplasma gondii. Out of 492, 113 sera (23.0%) reacted positively to either Nc-1 or KBA-2 strains of N. caninum. Among the 113 positive sera, 92 sera (81.4%) reacted with antigens of both strains, but 6 sera (5.3%) with Nc-1 and 15 sera (13.3%) with KBA-2 strain only. And with T. gondii antigen, 6 sera (1.2%) were positive but all reacted with N. caninum antigen also. Western blot revealed typical binding pattern according to ELISA values, such that high OD group reacted specifically to the major surface proteins including 43 kDa protein. Seroprevalence of 23.0% indicates that neosporosis seemed to be one of major causes of abortion in cattle. It is suggested here to establish more epidemiological researches nationwide systematically.

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Serological evaluation of Neospora caninum in pregnant women treated at referral center for prenatal screening in Mato Grosso do Sul, Brazil
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Veterinary Parasitology.2007; 143(1): 79. CrossRef
Epidemiology and Control of Neosporosis andNeospora caninum
J. P. Dubey, G. Schares, L. M. Ortega-Mora
Clinical Microbiology Reviews.2007; 20(2): 323. CrossRef
Diagnosis of bovine neosporosis
J.P. Dubey, G. Schares
Veterinary Parasitology.2006; 140(1-2): 1. CrossRef
ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera
Hye-Jin Ahn, Sera Kim, Dae-Yong Kim, Ho-Woo Nam
The Korean Journal of Parasitology.2003; 41(3): 175. CrossRef
Seroprevalence of Neospora, Toxoplasma gondii and Sarcocystis neurona antibodies in horses from Jeju island, South Korea
G.D Gupta, J Lakritz, Jae-Hoon Kim, Dae-Yong Kim, Jin-Kap Kim, A.E Marsh
Veterinary Parasitology.2002; 106(3): 193. CrossRef
Determination of antigenic domain in GST fused major surface protein (Nc-p43) of Neospora caninum
Eui-Sun Son, Hye-Jin Ahn, Jae-Hoon Kim, Dae-Yong Kim, Ho-Woo Nam
The Korean Journal of Parasitology.2001; 39(3): 241. CrossRef