Echinococcosis occurs mainly in areas with heavy livestock farming, such as Central Asia, America, and Australia. Echinococcus granulosus sensu lato (s.l.) infection causes echinococcosis in intermediate hosts, such as sheep, cattle, goats, camels, and horses. Numerous cases of echinococcosis occur in Uzbekistan as stock farming is a primary industry. Epidemiological and genetic studies of E. granulosus s.l. are very important for mitigating its impact on public health and the economy; however, there are no such studies on E. granulosus s.l. in Uzbekistan. In the present study, to determine which genotypes exist and are transmitted, we isolated Echinococcus sp. from definitive hosts (one isolate each from jackal and dog) and intermediate hosts (52 isolates from humans and 6 isolates from sheep) in Uzbekistan and analyzed the isolates by sequencing 2 mitochondrial DNA components (cox1 and nad1). The results showed that all of isolates except one belonged to the E. granulosus sensu stricto (s.s.) G1 and G3 genotypes. Phylogenetic analysis based on cox1 sequences showed that 42 isolates from humans, 6 isolates from sheep, and one isolate from jackal were the G1 genotype, whereas the remaining 8 isolates from human and the one isolate from dog were the G3 genotype. These results suggest that the G1 and G3 genotypes of E. granulosus s.s. are predominant in Uzbekistan, and both wild animals and domestic animals are important for maintaining their life cycle. Only one isolate from human sample was confirmed to be E. eqiinus (G4 genotype), which is known to be for the first time.
Citations
Citations to this article as recorded by
Cystic echinococcosis due to Echinococcus equinus in a Swiss donkey Isabelle L. Piotrowski, Rosalie Fabian, Stefanie M. Ohlerth, Felix Grimm, Meret E. Wehrli Eser Equine Veterinary Education.2025;[Epub] CrossRef
Morphological and molecular investigation of hydatid cyst isolated from small ruminants in Rasht, Iran Saeed Farokhpey, Soheil Sadr, Saeed Yaghfoori, Hassan Borji Journal of Parasitic Diseases.2025; 49(4): 944. CrossRef
Echinococcus species in wildlife Thomas Romig, Marion Wassermann International Journal for Parasitology: Parasites and Wildlife.2024; 23: 100913. CrossRef
Evaluation of the Molecular Properties of Echinococcus granulosus Isolates from Various Hosts in Şanlıurfa Province Cemile Günbegi Çalışkan, Fadile Yıldız Zeyrek Journal of Anatolian Environmental and Animal Sciences.2024; 9(3): 383. CrossRef
Cystic echinococcosis in donkeys in eastern Africa Erastus Mulinge, Eberhard Zeyhle, Cecilia Mbae, Lucy Gitau, Timothy Kaburu, Japhet Magambo, Ute Mackenstedt, Thomas Romig, Peter Kern, Marion Wassermann Parasitology.2023; 150(5): 468. CrossRef
Genotyping of Echinococcus granulosus isolated from canine in Northwest Iran Zabih Zarei, Behnam Mohammadi Ghalehbin, Behnaz Akhoundi, Mehdi Mohebali, Zahra Heidari Journal of Parasitic Diseases.2023; 47(4): 757. CrossRef
Genetic diversity of
Echinococcus granulosus
sensu lato in China: Epidemiological studies and systematic review
Rui‐Qi Hua, Xiao‐Di Du, Xue He, Xiao‐Bin Gu, Yue Xie, Ran He, Jing Xu, Xue‐Rong Peng, Guang‐You Yang Transboundary and Emerging Diseases.2022;[Epub] CrossRef
Species and genotypes belonging to Echinococcus granulosus sensu lato complex causing human cystic echinococcosis in Europe (2000–2021): a systematic review Adriano Casulli, Alessandro Massolo, Urmas Saarma, Gérald Umhang, Federica Santolamazza, Azzurra Santoro Parasites & Vectors.2022;[Epub] CrossRef
Insights into Human Cystic Echinococcosis in the Kurdistan Region, Iraq: Characteristics and Molecular Identification of Cysts Araz Ramadhan Issa, Sardar Hassan Arif, Ayad Ahmad Mohammed, Federica Santolamazza, Azzurra Santoro, Wijdan Mohammed Salih Mero, Adriano Casulli Pathogens.2022; 11(4): 408. CrossRef
Global distribution of Echinococcus granulosus genotypes in domestic and wild canids: a systematic review and meta-analysis Morteza Shams, Sasan Khazaei, Razi Naserifar, Seyyed Ali Shariatzadeh, Davood Anvari, Fattaneh Montazeri, Majid Pirestani, Hamidreza Majidiani Parasitology.2022; 149(9): 1147. CrossRef
Food-borne zoonotic echinococcosis: A review with special focus on epidemiology Mughees Aizaz Alvi, Abdullah F. Alsayeqh Frontiers in Veterinary Science.2022;[Epub] CrossRef
Molecular Epidemiology of Cystic Echinococcosis in Rural Baluchistan, Pakistan: A Cross-Sectional Study Ihsan Ullah, Sadia Sattar, Ijaz Ali, Arshad Farid, Amin Ullah, Refaat A. Eid, Mohamed Samir A. Zaki, Muhammad Alaa Eldeen, Iftikhar Ahmed, Irfan Ullah Pathogens.2022; 12(1): 40. CrossRef
Relevance of Human Leukocyte Antigen (HLA) class ll alleles in susceptibility or resistance to Echinococcus granulosus infection. Systematic review Facundo Martín Cardozo, Andrea Florencia Maglioco Data and Metadata.2022; 1: 37. CrossRef
A case of human cystic echinococcosis acquired in Ireland Caroline Conlon, Conor Grant, Helen Tuite, Catherine Fleming, Eoin Slattery Parasitology International.2021; 81: 102249. CrossRef
Occurrence of Echinococcusgranulosussensulato and Other Taeniids in Bhutan Puspa M. Sharma, Nirmal K. Thapa, Pema Tshomo, Tshewang Dema, Cristian A. Alvarez Rojas, Tenzin Tenzin, Ratna B. Gurung, Tshering Norbu, Lhatru Lhatru, Phurpa Namgyel, Chimi Jamtsho, Kinzang Dukpa, Yoenten Phuentshok, Krishna P. Sharma, Sonam Pelden, Pete Pathogens.2021; 10(3): 330. CrossRef
Cystic echinococcosis in sheep and goats of Lebanon Gaelle Joanny, Naunain Mehmood, Giorgia Dessì, Claudia Tamponi, Francesca Nonnis, Chadi Hosri, Urmas Saarma, Antonio Varcasia, Antonio Scala Parasitology.2021; 148(7): 871. CrossRef
Pulmonary hydatidosis genotypes isolates from human clinical surgery based on sequencing of mitochondrial genes in Fars, Iran Parviz Mardani, Ali Talebi Ezabadi, Bahareh Sedaghat, Seyed Mahmoud Sadjjadi Journal of Cardiothoracic Surgery.2021;[Epub] CrossRef
An annotated checklist of the eukaryotic parasites of humans, exclusive of fungi and algae Blaine A. Mathison, Sarah G. H. Sapp ZooKeys.2021; 1069: 1. CrossRef
A validated method to identify Echinococcus granulosus sensu lato at species level F. Santolamazza, A. Santoro, A. Possenti, S.M. Cacciò, A. Casulli Infection, Genetics and Evolution.2020; 85: 104575. CrossRef
A 2-year-old female donkey (Equus asinus) was euthanized in the Pathology Department of Firat University, Elazig, Turkey. Necropsy disclosed the presence of 7 hydatid cysts distributed throughout the lung parenchyma. One of those cysts represented the parasite material of the present study and was molecularly identified through sequencing of a fragment of cytochrome c oxidase subunit 1 (CO1) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 (NADH1) gene, as Echinococcus equinus. The generated CO1 sequence supports the presence of the dominant haplotype as has been described in Europe and Africa. The NADH1 sequence was found similar to sequences reported in equids in Egypt and the United Kingdom. The molecular identification of E. equinus in a donkey is being reported for the first time in Turkey.
Citations
Citations to this article as recorded by
Multifaceted analysis of equine cystic echinococcosis: genotyping, immunopathology, and screening of repurposed drugs against E. equinus protoscolices Noha Madbouly Taha, Mai A. Salem, Mohamed A. El-Saied, Faten F. Mohammed, Mohamed Kamel, Mohamed M. El-Bahy, Reem M. Ramadan BMC Veterinary Research.2025;[Epub] CrossRef
Echinococcus granulosus sensu stricto’nun G1 ve G3 Genotiplerinin Ayrımında PZR-RFLP ve SSCP Metotlarının Eş Zamanlı Kullanımı Figen Çelik, Muhammed Ahmed Selçuk, Muhammet Usluğ, Afra Sena Tekin, Sami Şimşek Dicle Üniversitesi Veteriner Fakültesi Dergisi.2025; 18(1): 28. CrossRef
Genetic Characterization of Echinococcus granulosus sensu stricto from Ruminants in Greece. Comparison with Findings in Türkiye and Reports from the Balkans and Mediterranean Countries Erifylli Roinioti, Ioannis Bossis, Sami Simsek Acta Parasitologica.2025;[Epub] CrossRef
Histopathological and molecular evaluation of hydatid cysts according to necropsy findings of 52 equids in Türkiye F. Celik, B. Karabulut, M. Uslug, E. Cankaya, Y. Eroksuz, S. Simsek Journal of Helminthology.2025;[Epub] CrossRef
Molecular discrimination of G1 and G3 genotypes of Echinococcus granulosus sensu stricto obtained from human, cattle, and sheep using the mitochondrial NADH dehydrogenase subunit 5 marker Figen Celik, Muhammed Ahmed Selcuk, Seyma Gunyakti Kilinc, Harun Kaya Kesik, Haroon Ahmed, Ying Wang, Sami Simsek, Jianping Cao Acta Tropica.2024; 252: 107124. CrossRef
Molecular genotyping of Echinococcus granulosus sensu stricto from human Echinococcal cysts in Hatay, Türkiye Berna Hamamcı, Günes Açıkgöz, Ülfet Çetinkaya, Erol Kılıç, Sedat Koçal, Kerem Karaaslan, Tülin Durgun Yetim, İbrahim Yetim Experimental Parasitology.2023; 245: 108454. CrossRef
Cystic echinococcosis in donkeys in eastern Africa Erastus Mulinge, Eberhard Zeyhle, Cecilia Mbae, Lucy Gitau, Timothy Kaburu, Japhet Magambo, Ute Mackenstedt, Thomas Romig, Peter Kern, Marion Wassermann Parasitology.2023; 150(5): 468. CrossRef
The situation of echinococcosis in stray dogs in Turkey: the first finding ofEchinococcus multilocularisandEchinococcus ortleppi Hamza Avcioglu, Esin Guven, Ibrahim Balkaya, Ridvan Kirman, Muzaffer Akyuz, Mohammed Mebarek Bia, Hatice Gulbeyen, Sali Yaya Parasitology.2021; 148(9): 1092. CrossRef
Echinococcoses in Iran, Turkey, and Pakistan: Old Diseases in the New Millennium Mehdi Borhani, Saeid Fathi, Enayat Darabi, Fatemeh Jalousian, Sami Simsek, Haroon Ahmed, Harun Kaya Kesik, Seyed Hossein Hosseini, Thomas Romig, Majid Fasihi Harandi, Iraj Mobedi Clinical Microbiology Reviews.2021;[Epub] CrossRef
Molecular Characterization of Echinococcus multilocularis and Echinococcus granulosus from Cysts and Formalin-Fixed Paraffin-Embedded Tissue Samples of Human Isolates in Northeastern Turkey Ali Kurt, Hamza Avcioglu, Esin Guven, Ibrahim Balkaya, Akgün Oral, Ridvan Kirman, Mohammed Mebarek Bia, Muzaffer Akyuz Vector-Borne and Zoonotic Diseases.2020; 20(8): 593. CrossRef
Occurrence of Liver Hydatid Cysts in a Donkey and Molecular Characterization of Echinococcus equinus Harun Kaya Kesik, Seyma Gunyakti Kilinc, Sami Simsek, Abdurrahman Gul Journal of Parasitology.2019; 105(3): 442. CrossRef
High-resolution phylogeography of zoonotic tapewormEchinococcus granulosussensu stricto genotype G1 with an emphasis on its distribution in Turkey, Italy and Spain LIINA KINKAR, TEIVI LAURIMÄE, SAMI SIMSEK, IBRAHIM BALKAYA, ADRIANO CASULLI, MARIA TERESA MANFREDI, FRANCISCO PONCE-GORDO, ANTONIO VARCASIA, ANTTI LAVIKAINEN, LUIS MIGUEL GONZÁLEZ, STEFFEN REHBEIN, JOKE VAN DER GIESSEN, HEIN SPRONG, URMAS SAARMA Parasitology.2016; 143(13): 1790. CrossRef
First
Prev
