Toxoplasmosis is an important zoonotic disease that can cause abortion in humans and animals. The aim of this study was isolation and subsequent genotyping of Toxolasma gondii isolates in ovine aborted fetuses. During 2012-2013, 39 ovine aborted fetuses were collected from sheep flocks in Khorasan Razavi Province, Iran. The brain samples were screened for detection of the parasite DNA by nested PCR. The positive brain samples were bioassayed in Webster Swiss mice. The serum samples of mice were examined for T. gondii antibodies by IFAT at 6 weeks post inoculation, and T. gondii cysts were searched in brain tissue samples of seropositive mice. The positive samples were genotyped by using a PCR-RLFP method. Subsequently, GRA6 sequences of isolates were analyzed using a phylogenetic method. The results revealed that T. gondii DNA was detected in 54% (20/37, 95% CI 38.4-69.0%) brain samples of ovine aborted fetuses. In bioassay of mice, only 2 samples were virulent and the mice were killed at 30 days post inoculation, while the others were non-virulent to mice. The size of cysts ranged 7-22 ?m. Complete genotyping data for GRA6 locus were observed in 5 of the 20 samples. PCR-RLFP results and phylogenetic analysis revealed that all of the isolated samples were closely related to type I. For the first time, we could genotype and report T. gondii isolates from ovine aborted fetuses in Khorasan Razavi Province, Iran. The results indicate that the T. gondii isolates are genetically related to type I, although most of them were non-virulent for mice.
Citations
Citations to this article as recorded by
Unraveling the link: serological and molecular insights into Toxoplasma gondii infection in women with spontaneous abortion history Narges Arbabi, Nima Firouzeh, Seyed Ghader Azizi, Ahmad Mehravaran, Soudabeh Etemadi, Reza Shafiei, Hadi Mirahmadi Tropical Diseases, Travel Medicine and Vaccines.2025;[Epub] CrossRef
Is GRA6 Gene a Suitable Marker for Molecular Typing of Toxoplasma Gondii? A Scoping Systematic Review Rohallah Abedian, Bahman Rahimi Esboei, Mahdi Fakhar, Zahra Rahmani, Mahbobeh Montazeri, Mostafa Solaymani, Hajar Ziaei Hezarjaribi Acta Parasitologica.2024; 69(1): 175. CrossRef
Serological and molecular survey of Toxoplasma Gondii in aborted livestock fetuses from Northeast Iran Reza Shafiei, Nima Firouzeh, Mohammad Taghi Rahimi BMC Research Notes.2024;[Epub] CrossRef
Toxoplasmosis diagnostic techniques: Current developed methods and biosensors Soheila Molaei, Masoomeh Dadkhah, Farzaneh Fathi Talanta.2023; 252: 123828. CrossRef
Prevalence and risk factors of Toxoplasma gondii infection among women with miscarriage and their aborted fetuses in the northwest of Iran Shiva Zeinali, Shahram Khademvatan, Rasool Jafari, Shabnam Vazifekhah, Elham Yousefi, Tahereh Behroozi-Lak, Bibi Razieh Hosseini Farash PLOS ONE.2023; 18(10): e0283493. CrossRef
Investigation of Toxoplasma gondii and Neospora caninum as cause of ovine abortion in affected flocks of Urmia, northwest of Iran A. Khodadadi, F. Malekifard, R. A. Batavani BULGARIAN JOURNAL OF VETERINARY MEDICINE.2022; 25(2): 308. CrossRef
Molecular and serological detection and of Toxoplasma gondii in small ruminants of southwest Iran and the potential risks for consumers Amin Yousefvand, Seyed Ali Mirhosseini, Masoud Ghorbani, Tahereh Mohammadzadeh, Mehrdad Moosazadeh Moghaddam, Shirin Mohammadyari Journal of Consumer Protection and Food Safety.2021; 16(2): 117. CrossRef
Global prevalence of Toxoplasma gondii infection in the aborted fetuses and ruminants that had an abortion: A systematic review and meta-analysis Tooran Nayeri, Shahabeddin Sarvi, Mahmood Moosazadeh, Ahmad Daryani Veterinary Parasitology.2021; 290: 109370. CrossRef
Molecular detection of Toxoplasma gondii in aborted fetuses of goats in Chattogram, Bangladesh Tanjila Hasan, Abdul Mannan, Delower Hossain, Azizunnesa Rekha, Md. Monir Hossan, Mohammad Abdul Alim, AHM Musleh Uddin Veterinary World.2021; : 2386. CrossRef
Molecular Diagnosis and Pathological Study of Toxoplasma gondii in Aborted Caprine and Ovine Fetuses in Borderline of Iran–Iraq Arjang Partoandazanpoor, Zainab Sadeghi-Dehkordi, Loghman Ekradi, Monireh Khordadmehr, Maryam Rassouli, Alireza Sazmand Acta Parasitologica.2020; 65(1): 187. CrossRef
WITHDRAWN: Economic and public health importance of Toxoplasma gondii infections in sheep: the last decade J.P. Dubey, F.H.A. Murata, C.K. Cerqueira-Cézar, O.C.H. Kwok, C. Su Veterinary Parasitology: X.2020; : 100028. CrossRef
Economic and public health importance of Toxoplasma gondii infections in sheep: 2009–2020 J.P. Dubey, F.H.A. Murata, C.K. Cerqueira-Cézar, O.C.H. Kwok, C. Su Veterinary Parasitology.2020; 286: 109195. CrossRef
Lack of circulating Toxoplasma gondii DNA in seropositive patients with bipolar or schizophrenia spectrum disorders. L. Galli, C. Del Grande, L. Rindi, C. Mangia, V. Mangano, E. Schiavi, I. Masci, B. Pinto, L. Kramer, L. Dell'Osso, F. Bruschi Psychiatry Research.2019;[Epub] CrossRef
Toxoplasma gondii infection and toxoplasmosis in farm animals: Risk factors and economic impact S. Stelzer, W. Basso, J. Benavides Silván, L.M. Ortega-Mora, P. Maksimov, J. Gethmann, F.J. Conraths, G. Schares Food and Waterborne Parasitology.2019; 15: e00037. CrossRef
Detection of Toxoplasma gondii genotypes in abortion women by RFLP-PCR in Al-Najaf Al-Ashraf province Saleem Khteer Al-Hadraawy, Mohammad Alzeyadi, Ameer Ali Shakir, Arshad noori Al-dujaili Journal of Physics: Conference Series.2019; 1234(1): 012084. CrossRef
Molecular genotyping and serological evaluation of Toxoplasma gondii in mothers and their spontaneous aborted fetuses in Southwest of Iran Nasir Arefkhah, Bahman Pourabbas, Qasem Asgari, Abdolali Moshfe, Fataneh Mikaeili, Gordafarin Nikbakht, Bahador Sarkari Comparative Immunology, Microbiology and Infectious Diseases.2019; 66: 101342. CrossRef
Serological and molecular detection ofToxoplasma gondiiin sheep and goats in Kashan, Central Iran Sima Rasti, Nader Marandi, Amir Abdoli, Mahdi Delavari, Seyed Gholam Abbas Mousavi Journal of Food Safety.2018;[Epub] CrossRef
Prevalence and molecular characterization of Toxoplasma gondii DNA in retail fresh meats in Canada Asma Iqbal, Nicol Janecko, Frank Pollari, Brent Dixon Food and Waterborne Parasitology.2018; 13: e00031. CrossRef
Toxoplasma gondii Type I, predominant genotype isolated from sheep in South of Iran Belal Armand, Kavous Solhjoo, Manoochehr Shabani Kordshooli, Mohammad Hasan Davami, Morteza Pourahmad, Vahideh Orfaee Veterinary World.2017; 10(4): 386. CrossRef
Seroprevalence and genetic characterization of Toxoplasma gondii in masked palm civet ( Paguma larvata ) in Hainan province, tropical China Guan-Yu Hou, Jun-Ming Zhao, Han-Lin Zhou, Guang Rong Acta Tropica.2016; 162: 103. CrossRef
Neospora caninum is an important cause of abortion in dairy cattle worldwide. Dog is the definitive host for N. caninum and can infect dairy cattle. The aim of this study is to determine the prevalence of Neospora oocysts in feces of dogs from dairy farms. A total of 174 fecal samples was collected from 89 farm dogs and 85 household dogs during 2006 and 2008. Fecal samples of dogs were microscopically examined for detecting Hammondia Neospora-like oocysts (HNLO) by Mini Parasep®SF fecal parasite concentrator. HNLO were microscopically detected in 4 fecal samples (2.2%). The fecal samples with HNLO were examined by N. caninum-specific PCR. Two of the samples were positive for N. caninum. The 2 positive fecal samples were selected for inoculation to calves. Two inoculated calves were seronegative by ELISA for 4 months post-infection. This is the first report of finding N. caninum DNA in feces of farm dogs in Mashhad area, Iran.
Citations
Citations to this article as recorded by
Molecular Detection of Neospora Caninum in Different Tissues of Dogs and Cats in Shiraz, Iran Gholam Reza Hatam, Fattaneh Mikaeili, Qasem Asgari, Mehdi Namavari, Shamsi Noorpisheh Ghadimi Veterinary Medicine and Science.2025;[Epub] CrossRef
Molecular detection and phylogenetic analysis of Neospora caninum in various hosts from Iran Jamal Gharekhani, Mohammad Yakhchali, Reza Heidari Comparative Immunology, Microbiology and Infectious Diseases.2022; 80: 101737. CrossRef
Incorrect reports of Eimeria spp. from dogs—Veterinary and medical parasitologists should work closer Alireza Sazmand, Alireza Nourian Comparative Immunology, Microbiology and Infectious Diseases.2020; 69: 101420. CrossRef
Neospora caninum infection in Iran (2004–2020): A review Jamal Gharekhani, Mohammad Yakhchali, Reza Berahmat Journal of Parasitic Diseases.2020; 44(4): 671. CrossRef
Prevalence and Risk Factors Associated to Neospora caninum (Apicomplexa: Toxoplasmatidae) in Pet Dogs From Hamadan, West of Iran, 2016 Jamal Gharekhani, Mohammad Yakhchali, Reza Khaltabadi-Farahani Avicenna Journal of Clinical Microbiology and Infection.2020; 7(1): 22. CrossRef
Risk of environmental exposure to small coccidia from wild canid feces in rural Ohio Karla I. Moreno-Torres, Devinn M. Sinnott, Barbara A. Wolfe, Antoinette E. Marsh, William J. A. Saville, Mark Moritz, Rebecca B. Garabed American Journal of Veterinary Research.2018; 79(11): 1179. CrossRef
Development of a loop-mediated isothermal amplification technique and comparison with quantitative real-time PCR for the rapid visual detection of canine neosporosis Aongart Mahittikorn, Nipa Thammasonthijarern, Amonrattana Roobthaisong, Ruenruetai Udonsom, Supaluk Popruk, Sukhontha Siri, Hirotake Mori, Yaowalark Sukthana Parasites & Vectors.2017;[Epub] CrossRef
Occurrence and first multilocus microsatellite genotyping of Neospora caninum from naturally infected dogs in dairy farms in Henan, Central China Weifeng Qian, Tianqi Wang, Wenchao Yan, Lifang Han, Kai Zhai, Baoqing Duan, Chaochao Lv Parasitology Research.2016; 115(8): 3267. CrossRef
The detection and characterisation of Neospora /Hammondia-like oocysts from naturally infected dogs within the same urban region of Australia Amanda Ash, Aileen Elliot, R.C. Andrew Thompson Veterinary Parasitology: Regional Studies and Reports.2015; 1-2: 47. CrossRef
Molecular identification of Neospora caninum from calf/foetal brain tissue and among oocysts recovered from faeces of naturally infected dogs in southern Ethiopia K. Asmare, E. Skjerve, J. Bekele, D. Sheferaw, T. Stachurska-Hagen, L.J. Robertson Acta Tropica.2014; 130: 88. CrossRef
Histopathological and molecular study of Neospora caninum infection in bovine aborted fetuses Amir Kamali, Hesam Adin Seifi, Ahmad Reza Movassaghi, Gholam Reza Razmi, Zahra Naseri Asian Pacific Journal of Tropical Biomedicine.2014; 4(12): 990. CrossRef
Neosporosis in animals—The last five years J.P. Dubey, G. Schares Veterinary Parasitology.2011; 180(1-2): 90. CrossRef
Neospora caninum infection in stray and farm dogs: Seroepidemiological study and oocyst shedding J. Regidor-Cerrillo, S. Pedraza-Diaz, S. Rojo-Montejo, E. Vazquez-Moreno, I. Arnaiz, M. Gomez-Bautista, S. Jimenez-Palacios, L.M. Ortega-Mora, E. Collantes-Fernandez Veterinary Parasitology.2010; 174(3-4): 332. CrossRef
First
Prev
