Echinococcosis occurs mainly in areas with heavy livestock farming, such as Central Asia, America, and Australia. Echinococcus granulosus sensu lato (s.l.) infection causes echinococcosis in intermediate hosts, such as sheep, cattle, goats, camels, and horses. Numerous cases of echinococcosis occur in Uzbekistan as stock farming is a primary industry. Epidemiological and genetic studies of E. granulosus s.l. are very important for mitigating its impact on public health and the economy; however, there are no such studies on E. granulosus s.l. in Uzbekistan. In the present study, to determine which genotypes exist and are transmitted, we isolated Echinococcus sp. from definitive hosts (one isolate each from jackal and dog) and intermediate hosts (52 isolates from humans and 6 isolates from sheep) in Uzbekistan and analyzed the isolates by sequencing 2 mitochondrial DNA components (cox1 and nad1). The results showed that all of isolates except one belonged to the E. granulosus sensu stricto (s.s.) G1 and G3 genotypes. Phylogenetic analysis based on cox1 sequences showed that 42 isolates from humans, 6 isolates from sheep, and one isolate from jackal were the G1 genotype, whereas the remaining 8 isolates from human and the one isolate from dog were the G3 genotype. These results suggest that the G1 and G3 genotypes of E. granulosus s.s. are predominant in Uzbekistan, and both wild animals and domestic animals are important for maintaining their life cycle. Only one isolate from human sample was confirmed to be E. eqiinus (G4 genotype), which is known to be for the first time.
Citations
Citations to this article as recorded by
Cystic echinococcosis due to Echinococcus equinus in a Swiss donkey Isabelle L. Piotrowski, Rosalie Fabian, Stefanie M. Ohlerth, Felix Grimm, Meret E. Wehrli Eser Equine Veterinary Education.2025;[Epub] CrossRef
Morphological and molecular investigation of hydatid cyst isolated from small ruminants in Rasht, Iran Saeed Farokhpey, Soheil Sadr, Saeed Yaghfoori, Hassan Borji Journal of Parasitic Diseases.2025; 49(4): 944. CrossRef
Echinococcus species in wildlife Thomas Romig, Marion Wassermann International Journal for Parasitology: Parasites and Wildlife.2024; 23: 100913. CrossRef
Evaluation of the Molecular Properties of Echinococcus granulosus Isolates from Various Hosts in Şanlıurfa Province Cemile Günbegi Çalışkan, Fadile Yıldız Zeyrek Journal of Anatolian Environmental and Animal Sciences.2024; 9(3): 383. CrossRef
Cystic echinococcosis in donkeys in eastern Africa Erastus Mulinge, Eberhard Zeyhle, Cecilia Mbae, Lucy Gitau, Timothy Kaburu, Japhet Magambo, Ute Mackenstedt, Thomas Romig, Peter Kern, Marion Wassermann Parasitology.2023; 150(5): 468. CrossRef
Genotyping of Echinococcus granulosus isolated from canine in Northwest Iran Zabih Zarei, Behnam Mohammadi Ghalehbin, Behnaz Akhoundi, Mehdi Mohebali, Zahra Heidari Journal of Parasitic Diseases.2023; 47(4): 757. CrossRef
Genetic diversity of
Echinococcus granulosus
sensu lato in China: Epidemiological studies and systematic review
Rui‐Qi Hua, Xiao‐Di Du, Xue He, Xiao‐Bin Gu, Yue Xie, Ran He, Jing Xu, Xue‐Rong Peng, Guang‐You Yang Transboundary and Emerging Diseases.2022;[Epub] CrossRef
Species and genotypes belonging to Echinococcus granulosus sensu lato complex causing human cystic echinococcosis in Europe (2000–2021): a systematic review Adriano Casulli, Alessandro Massolo, Urmas Saarma, Gérald Umhang, Federica Santolamazza, Azzurra Santoro Parasites & Vectors.2022;[Epub] CrossRef
Insights into Human Cystic Echinococcosis in the Kurdistan Region, Iraq: Characteristics and Molecular Identification of Cysts Araz Ramadhan Issa, Sardar Hassan Arif, Ayad Ahmad Mohammed, Federica Santolamazza, Azzurra Santoro, Wijdan Mohammed Salih Mero, Adriano Casulli Pathogens.2022; 11(4): 408. CrossRef
Global distribution of Echinococcus granulosus genotypes in domestic and wild canids: a systematic review and meta-analysis Morteza Shams, Sasan Khazaei, Razi Naserifar, Seyyed Ali Shariatzadeh, Davood Anvari, Fattaneh Montazeri, Majid Pirestani, Hamidreza Majidiani Parasitology.2022; 149(9): 1147. CrossRef
Food-borne zoonotic echinococcosis: A review with special focus on epidemiology Mughees Aizaz Alvi, Abdullah F. Alsayeqh Frontiers in Veterinary Science.2022;[Epub] CrossRef
Molecular Epidemiology of Cystic Echinococcosis in Rural Baluchistan, Pakistan: A Cross-Sectional Study Ihsan Ullah, Sadia Sattar, Ijaz Ali, Arshad Farid, Amin Ullah, Refaat A. Eid, Mohamed Samir A. Zaki, Muhammad Alaa Eldeen, Iftikhar Ahmed, Irfan Ullah Pathogens.2022; 12(1): 40. CrossRef
Relevance of Human Leukocyte Antigen (HLA) class ll alleles in susceptibility or resistance to Echinococcus granulosus infection. Systematic review Facundo Martín Cardozo, Andrea Florencia Maglioco Data and Metadata.2022; 1: 37. CrossRef
A case of human cystic echinococcosis acquired in Ireland Caroline Conlon, Conor Grant, Helen Tuite, Catherine Fleming, Eoin Slattery Parasitology International.2021; 81: 102249. CrossRef
Occurrence of Echinococcusgranulosussensulato and Other Taeniids in Bhutan Puspa M. Sharma, Nirmal K. Thapa, Pema Tshomo, Tshewang Dema, Cristian A. Alvarez Rojas, Tenzin Tenzin, Ratna B. Gurung, Tshering Norbu, Lhatru Lhatru, Phurpa Namgyel, Chimi Jamtsho, Kinzang Dukpa, Yoenten Phuentshok, Krishna P. Sharma, Sonam Pelden, Pete Pathogens.2021; 10(3): 330. CrossRef
Cystic echinococcosis in sheep and goats of Lebanon Gaelle Joanny, Naunain Mehmood, Giorgia Dessì, Claudia Tamponi, Francesca Nonnis, Chadi Hosri, Urmas Saarma, Antonio Varcasia, Antonio Scala Parasitology.2021; 148(7): 871. CrossRef
Pulmonary hydatidosis genotypes isolates from human clinical surgery based on sequencing of mitochondrial genes in Fars, Iran Parviz Mardani, Ali Talebi Ezabadi, Bahareh Sedaghat, Seyed Mahmoud Sadjjadi Journal of Cardiothoracic Surgery.2021;[Epub] CrossRef
An annotated checklist of the eukaryotic parasites of humans, exclusive of fungi and algae Blaine A. Mathison, Sarah G. H. Sapp ZooKeys.2021; 1069: 1. CrossRef
A validated method to identify Echinococcus granulosus sensu lato at species level F. Santolamazza, A. Santoro, A. Possenti, S.M. Cacciò, A. Casulli Infection, Genetics and Evolution.2020; 85: 104575. CrossRef
Echinococcus granulosus sensu lato (s.l.) is a causative agent of cystic echinococcosis or cystic hydatid disease in humans and domestic and wild animals. The disease is a serious health problem in countries associated with poverty and poor hygiene practices, particularly in livestock raising. We introduced a practical algorism for genotyping the parasite, which may be useful to many developing countries. To evaluate the efficiency of the algorism, we genotyped 3 unknown strains isolated from human patients. We found that unknowns 1 and 3 were included in G1, G2, and G3 genotypes group and unknown 2 was included in G4 genotype (Echinococcus equinus) according to the algorisms. We confirmed
these results by sequencing the 3 unknown isolates cox1 and nad1 PCR products. In conclusion, these new algorisms are very fast genotype identification tools that are suitable for evaluating E. granulosus s.l. isolated from livestock or livestock holders, particularly in developing countries.
Citations
Citations to this article as recorded by
Primary cerebral cystic echinococcosis in a child from Roman countryside: Source attribution and scoping review of cases from the literature Adriano Casulli, Stefania Pane, Franco Randi, Paola Scaramozzino, Andrea Carvelli, Carlo Efisio Marras, Andrea Carai, Azzurra Santoro, Federica Santolamazza, Francesca Tamarozzi, Lorenza Putignani, Solange Bresson-Hadni PLOS Neglected Tropical Diseases.2023; 17(9): e0011612. CrossRef
A validated method to identify Echinococcus granulosus sensu lato at species level F. Santolamazza, A. Santoro, A. Possenti, S.M. Cacciò, A. Casulli Infection, Genetics and Evolution.2020; 85: 104575. CrossRef
A pilot comparative study between serological and genetic investigations in relationship to clinical outcomes on patients with cystic echinococcosis Nu. Altintas, S. A. Karamil, O. Turkum, M. Akil, A. Sakarya, H. Bozkaya, J. Šoltys, Na. Altintas Helminthologia.2020; 57(2): 91. CrossRef
Evaluation of PCR‐HRM, RFLP, and direct sequencing as simple and cost‐effective methods to detect common EGFR mutations in plasma cell–free DNA of non–small cell lung cancer patients Jamal Zaini, Elisna Syahruddin, Muhammad Yunus, Sita Laksmi Andarini, Achmad Hudoyo, Najmiatul Masykura, Refniwita Yasril, Asep Ridwanuloh, Heriawaty Hidajat, Fariz Nurwidya, Sony Suharsono, Ahmad R.H. Utomo Cancer Reports.2019;[Epub] CrossRef
First
Prev
