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"Hyeong Woo Lee"

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"Hyeong Woo Lee"

Original Articles
Detection of vivax malaria sporozoites naturally infected in Anopheline mosquitoes from endemic areas of northern parts of Gyeonggi-do (Province) in Korea
Hyeong Woo Lee, E-Hyun Shin, Shin Hyeong Cho, Hee Il Lee, Chung Lim Kim, Wook Gyo Lee, Sung Ung Moon, Jong Soo Lee, Wan Ja Lee, Tong-Soo Kim
Korean J Parasitol 2002;40(2):75-81.
Published online June 30, 2002
DOI: https://doi.org/10.3347/kjp.2002.40.2.75

We investigated population densities of mosquitoes infected with sporozoites in three highly epidemic areas of Josan-ri and Jangpa-ri (Paju City) and Dongjung-ri (Yeoncheon County) in Korea. Anopheline mosquitoes were collected from both indoors and outdoors by human baiting collection method during the period of the first week of June to the second week of September 1999. Total 13,296 female mosquitoes were collected and 8,650 (65.1%) were Anophelines. Thirty seven percent (3,199) of the Anopheline mosquitoes were captured outdoors and 63.9% (5,531) indoors. Employing a sandwich enzyme-linked immunosorbent assay (ELISA), we analyzed a total of 7,820 Anopheline mosquitoes and found that 7 Anopheline mosquitoes were infected with sporozoites. The positive rate in Josan-ri was 0.14% (5/3,500) and 0.15% (2/1,370) in Jangpa-ri. The total positive rate in all three surveyed areas was 0.09% (7/7,820). The mosquitoes infected with the sporozoites were detected on June 28th (n=2), July 5th (n=1), July 19th (n=1), August 9th (n=1), September 6th (n=1), and the last one on September 13th (n=1). They were all classified as Anopheles sinensis, which showed positive reaction in ELISA test. Therefore it might be concluded that Anopheles sinensis plays an important role in re-emerging malaria transmission in Korea.

Citations

Citations to this article as recorded by  Crossref logo
  • Survey of malaria vectors on the Cambodia, Thailand and China-Laos Borders
    Canglin Zhang, Rui Yang, Linbo Wu, Chunhai Luo, Yaming Yang, Yan Deng, Jing Wu, Yan Liu, Hongning Zhou
    Malaria Journal.2022;[Epub]     CrossRef
  • Species diversity and insecticide resistance within the Anopheles hyrcanus group in Ubon Ratchathani Province, Thailand
    Anchana Sumarnrote, Hans J. Overgaard, Vincent Corbel, Kanutcharee Thanispong, Theeraphap Chareonviriyaphap, Sylvie Manguin
    Parasites & Vectors.2020;[Epub]     CrossRef
  • The polymorphism and geographical distribution of knockdown resistance of adult Anopheles sinensis populations in eastern China
    Wei-Long Tan, Chun-Xiao Li, Rui-Chen Lv, Yan-De Dong, Xiao-Xia Guo, Dan Xing, Ming-hao Zhou, Yan Xu, Hong-liang Chu, Gang Wang, Chang-qiang Zhu, Jun Sun, Tong-Yan Zhao
    Malaria Journal.2019;[Epub]     CrossRef
  • Cost-Benefit Analysis of Malaria Chemoprophylaxis and Early Diagnosis for Korean Soldiers in Malaria Risk Regions
    Hee-sung Kim, Gilwon Kang, Sunmi Lee, Chang-gyo Yoon, Minyoung Kim
    Journal of Korean Medical Science.2018;[Epub]     CrossRef
  • Seasonal dynamics ofAnophelesspecies at three locations in the Republic of Korea
    Desmond H. Foley, Terry A. Klein, Heung Chul Kim, Myung-Soon Kim, Richard C. Wilkerson, Cong Li, Genelle Harrison, Leopoldo M. Rueda
    Journal of Vector Ecology.2017; 42(2): 335.     CrossRef
  • Vector Competence ofAnopheles kleiniandAnopheles sinensis(Diptera: Culicidae) From the Republic of Korea to Vivax Malaria-Infected Blood From Patients From Thailand
    Ratawan Ubalee, Heung-Chul Kim, Anthony L. Schuster, Patrick W. McCardle, Siriporn Phasomkusolsil, Ratree Takhampunya, Silas A. Davidson, Won-Ja Lee, Terry A. Klein
    Journal of Medical Entomology.2016; 53(6): 1425.     CrossRef
  • Status of Plasmodium vivax malaria in the Republic of Korea, 2008–2009: decrease followed by resurgence
    Joon-Sup Yeom, Gyo Jun, Jung-Yeon Kim, Won-Ja Lee, E-Hyun Shin, Kyu-Sik Chang, Ji-Hwan Bang, Sejoong Oh, Joon-Young Kang, Jae-Won Park
    Transactions of the Royal Society of Tropical Medicine and Hygiene.2012; 106(7): 429.     CrossRef
  • First Detection of Multiple Knockdown Resistance (kdr)-Like Mutations in Voltage-Gated Sodium Channel Using Three New Genotyping Methods inAnopheles sinensisFrom Guangxi Province, China
    Wei L. Tan, Chun X. Li, Zhong M. Wang, Mei D. Liu, Yan D. Dong, Xiang Y. Feng, Zhi M. Wu, Xiao X. Guo, Dan Xing, Ying M. Zhang, Zhong C. Wang, Tong Y. Zhao
    Journal of Medical Entomology.2012; 49(5): 1012.     CrossRef
  • Preliminary Vivax Malaria Vector Competence for Three Members of the Anopheles Hyrcanus Group in the Republic of Korea
    Deepak Joshi, Jung-Yeon Kim, Wej Choochote, Mi-Hyun Park, Gi-Sik Min
    Journal of the American Mosquito Control Association.2011; 27(3): 312.     CrossRef
  • Molecular cloning and expression of the VK247 circumsporozoite protein for serodiagnosis of variant form Plasmodium vivax
    Tong-Soo Kim, Hyung-Hwan Kim, Sun-Sim Lee, Chang-Mi Oh, Kyung-Mi Choi, Khin Lin, Jung-Yeon Kim, Byoung-Kuk Na, Eun-Taek Han, Youngjoo Sohn, Hyuck Kim, Hyeong-Woo Lee
    Parasitology Research.2011; 108(5): 1275.     CrossRef
  • The dominant Anopheles vectors of human malaria in the Asia-Pacific region: occurrence data, distribution maps and bionomic précis
    Marianne E Sinka, Michael J Bangs, Sylvie Manguin, Theeraphap Chareonviriyaphap, Anand P Patil, William H Temperley, Peter W Gething, Iqbal RF Elyazar, Caroline W Kabaria, Ralph E Harbach, Simon I Hay
    Parasites & Vectors.2011;[Epub]     CrossRef
  • A review of mixed malaria species infections in anopheline mosquitoes
    Mallika Imwong, Supatchara Nakeesathit, Nicholas PJ Day, Nicholas J White
    Malaria Journal.2011;[Epub]     CrossRef
  • The role of Pvs28 in sporozoite development in Anopheles sinensis and its longevity in BALB/c mice
    Tong-Soo Kim, Hyung-Hwan Kim, Sung-Ung Moon, Sun-Sim Lee, E-Hyun Shin, Chang-Mi Oh, Yoon-Joong Kang, Do-Kyung Kim, Youngjoo Sohn, Hyuck Kim, Hyeong-Woo Lee
    Experimental Parasitology.2011; 127(2): 346.     CrossRef
  • Malaria Vector Surveillance in Ganghwa-do, a Malaria-Endemic Area in the Republic of Korea
    Sung Suck Oh, Myung Je Hur, Gwang Sig Joo, Sung Tae Kim, Jong Myoung Go, Yong Hee Kim, Wook Gyo Lee, E Hyun Shin
    The Korean Journal of Parasitology.2010; 48(1): 35.     CrossRef
  • Blocking Effect of a Monoclonal Antibody against Recombinant Pvs25 on Sporozoite Development in Anopheles sinensis
    Sung-Ung Moon, Hyung-Hwan Kim, Tong-Soo Kim, Kyung-Mi Choi, Chang-Mi Oh, Yong-Joo Ahn, Seo-Kyoung Hwang, Youngjoo Sohn, E-Hyun Shin, Hyuck Kim, Hyeong-Woo Lee
    Clinical and Vaccine Immunology.2010; 17(8): 1183.     CrossRef
  • The susceptibility of Anopheles lesteri to infection with Korean strain of Plasmodium vivax
    Deepak Joshi, Wej Choochote, Mi-Hyun Park, Jung-Yeon Kim, Tong-Soo Kim, Wannapa Suwonkerd, Gi-Sik Min
    Malaria Journal.2009;[Epub]     CrossRef
  • Detection of Malaria Parasites in Mosquitoes from the Malaria-Endemic Area of Chakaria, Bangladesh
    Akter Tangin, Yuka Komichi, Yukiko Wagatsuma, Haque Rashidul, Yusuke Wataya, Hye-Sook Kim
    Biological and Pharmaceutical Bulletin.2008; 31(4): 703.     CrossRef
  • Reemergence of Malaria in Korea
    Weon-Gyu Kho
    Journal of the Korean Medical Association.2007; 50(11): 959.     CrossRef
  • Seasonal Prevalence of Mosquitoes Collected from Light Traps in the Republic of Korea, 2002
    Heung Chul KIM, Sung Tae CHONG, Jason G. PIKE, Monica L. O'GUINN, Laura A. PACHA, Hee‐Choon S. LEE, Terry A. KLEIN
    Entomological Research.2004; 34(3): 177.     CrossRef
  • Seasonal Prevalence of Mosquitoes Collected from Light Traps in the Republic of Korea, 2001
    Heung Chul KIM, Oscar S. FRIENDLY, Jason G. PIKE, Anthony L. SCHUSTER, Monica L. O'GUINN, Terry A. KLEIN
    Entomological Research.2003; 33(3): 189.     CrossRef
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Experimental infection of Anopheles sinensis with Korean isolates of Plasmodium vivax
Hyeong Woo Lee, Shin Hyeong Cho, E-Hyun Shin, Jong Soo Lee, Joon-Sang Lee, Jong-Yil Chai, Soon-Hyung Lee, Tong Soo Kim
Korean J Parasitol 2001;39(2):177-183.
Published online June 30, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.2.177

The
objective
s of the present study were to (1) determine the susceptibility of Anopheles sinensis to Korean isolates of Plasmodium vivax, (2) establish a method to collect large quantities of P. vivax sporozoites for use as antigen in seroepidemiological studies, and (3) investigate the characteristics of Korean isolates of P. vivax sporozoites. Females of Anopheles sinensis were collected at non-epidemic area, Seokwha-ri, Cheongwon-gun and Chungcheongbuk-do using tent-trap methods coupled with dry ice. The females were artificially infected with gametocytes of P. vivax using blood obtained from P. vivax malaria patients. Individual mosquitoes were infected using either a parafilm-covered glass feeding apparatus or were allowed to feed on naturally infected volunteers. Mosquitoes were sacrificed between 16 and 18 days post-feeding and an enzyme-linked immunosorbent assay (ELISA) was used to detect sporozoites. Four (33.4%) of 12 mosquitoes, which were fed on naturally infected volunteers directly, were positive for sporozoites. In cases, the mosquitoes allowed to feed on whole blood which were extract from three different patients with heparin treated vacuutainers using a parafilm-covered glass apparatus. Two of 55 (3.6%) were positive which blood sample was maintained at room temperature for 8 hours, 1 of 68 (1.5%) was positive which blood was maintained at 4℃ for 24 hours and 1 of 47 (2.3%) was positive at 4℃ for 48 hours. The mean number of sporozoites was estimated about 818 (n=8; range of 648-1,056) based on optical density values of ELISA.

Citations

Citations to this article as recorded by  Crossref logo
  • 국내 말라리아 매개모기 집단유전학 분석
    하늘 정, 보경 한, 현일 신, 명노 이, 정원 주, 희일 이
    Public Health Weekly Report.2025; 18(16): 623.     CrossRef
  • Genetic diversity and spatiotemporal population structure of Anopheles sinensis in the Republic of Korea based on the mitochondrial cytochrome c oxidase subunit I (COI) marker
    Haneul Jung, Bo Gyeong Han, Hyun-Il Shin, Myoung-Ro Lee, Jung-Won Ju, Hee-Il Lee
    Osong Public Health and Research Perspectives.2025; 16(4): 348.     CrossRef
  • Plasmodium vivax gametocytes and transmission
    Sirasate Bantuchai, Hisham Imad, Wang Nguitragool
    Parasitology International.2022; 87: 102497.     CrossRef
  • Quality Change of Fermented Soybean Products by Aspergillus spp. from Soybean Cultivar
    Dong Sun Shin, In Duck Choi, Seuk Ki Lee, Ji Young Park, Nam Geol Kim, Kwang-Ho Jeong, Chang Hwan Park, Hye Sun Choi
    Food Engineering Progress.2019; 23(4): 258.     CrossRef
  • Epidemiological Characteristics of Re-emerging Vivax Malaria in the Republic of Korea (1993-2017)
    Young Yil Bahk, Hyeong-Woo Lee, Byoung-Kuk Na, Jeonga Kim, Kyoung Jin, Yeong Seon Hong, Tong-Soo Kim
    The Korean Journal of Parasitology.2018; 56(6): 531.     CrossRef
  • Singapore’s Anopheles sinensis Form A is susceptible to Plasmodium vivax isolates from the western Thailand–Myanmar border
    Sook-Cheng Pang, Chiara Andolina, Benoit Malleret, Peter R. Christensen, Sai-Gek Lam-Phua, Muhammad Aliff Bin Abdul Razak, Chee-Seng Chong, Daiqin Li, Cindy S. Chu, Bruce Russell, Laurent Rénia, Lee-Ching Ng, Francois Nosten
    Malaria Journal.2017;[Epub]     CrossRef
  • The dominant Anopheles vectors of human malaria in the Asia-Pacific region: occurrence data, distribution maps and bionomic précis
    Marianne E Sinka, Michael J Bangs, Sylvie Manguin, Theeraphap Chareonviriyaphap, Anand P Patil, William H Temperley, Peter W Gething, Iqbal RF Elyazar, Caroline W Kabaria, Ralph E Harbach, Simon I Hay
    Parasites & Vectors.2011;[Epub]     CrossRef
  • The role of Pvs28 in sporozoite development in Anopheles sinensis and its longevity in BALB/c mice
    Tong-Soo Kim, Hyung-Hwan Kim, Sung-Ung Moon, Sun-Sim Lee, E-Hyun Shin, Chang-Mi Oh, Yoon-Joong Kang, Do-Kyung Kim, Youngjoo Sohn, Hyuck Kim, Hyeong-Woo Lee
    Experimental Parasitology.2011; 127(2): 346.     CrossRef
  • The susceptibility of Anopheles lesteri to infection with Korean strain of Plasmodium vivax
    Deepak Joshi, Wej Choochote, Mi-Hyun Park, Jung-Yeon Kim, Tong-Soo Kim, Wannapa Suwonkerd, Gi-Sik Min
    Malaria Journal.2009;[Epub]     CrossRef
  • Anopheles kleini,Anopheles pullus, andAnopheles sinensis: Potential Vectors ofPlasmodium vivaxin the Republic of Korea
    Won-Ja Lee, Terry A. Klein, Heung-Chul Kim, Young-Mi Choi, Seok-Hyun Yoon, Kyu-Sik Chang, Sung-Tae Chong, In-Yong Lee, James W. Jones, Jolanta S. Jacobs, Jetsumon Sattabongkot, Jae-Sun Park
    Journal of Medical Entomology.2007; 44(6): 1086.     CrossRef
  • Anopheles kleini, Anopheles pullus, and Anopheles sinensis: Potential Vectors of Plasmodium vivax in the Republic of Korea
    Won-Ja Lee, Terry A. Klein, Heung-Chul Kim, Young-Mi Choi, Seok-Hyun Yoon, Kyu-Sik Chang, Sung-Tae Chong, In-Yong Lee, James W. Jones, Jolanta S. Jacobs, Jetsumon Sattabongkot, Jae-Sun Park
    Journal of Medical Entomology.2007; 44(6): 1086.     CrossRef
  • Detection of vivax sporozoites naturally infected in Anopheline mosquitoes from endemic areas of northern parts of Gyeonggi-do (province) in Korea
    Hyeong Woo Lee, E Hyun Shin, Shin Hyeong Cho, Hee Il Lee, Chung Lim Kim, Wook Gyo Lee, Sung Ung Moon, Jong Soo Lee, Wan Ja Lee, Tong Soo Kim
    The Korean Journal of Parasitology.2002; 40(2): 75.     CrossRef
  • Vector competence of Anopheles lesteri Baisas and Hu (Diptera: Culicidae) to Plasmodium vivax in Korea
    E-Hyun Shin, Tong-Soo Kim, Hyeong-Woo Lee, Jong-Soo Lee, Won-Ja Lee
    The Korean Journal of Parasitology.2002; 40(1): 41.     CrossRef
  • Intraspecific Hybridization of Anopheles sinensis (Diptera: Culicidae) Strains from Thailand and Korea
    Gi-Sik Min, Wej Choochote, Atchariya Jitpakdi, Se-Joo Kim, Won Kim, Jongwoo Jung, Anuluck Junkum
    Molecules and Cells.2002; 14(2): 198.     CrossRef
  • 8,901 View
  • 72 Download
  • Crossref