Parasites, Hosts and Diseases

"IgG-ELISA"

Case Report

Clinical Features of Ocular Toxoplasmosis in Korean Patients: Young-Hoon Park, Jae-Hyung Han, Ho-Woo Nam; Korean J Parasitol 2011;49(2):167-171.
Published online June 14, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.2.167

Abstract
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We report here the records of 10 consecutive Korean patients (10 eyes) with ocular toxoplasmosis which showed the typical clinical manifestations with seropositivity for Toxoplasma gondii specific IgG antibodies by micro-ELISA between 2006 and 2010. Nine patients were males and 1 was female; their age was 50.5±13.8 years. The most common accompanying signs were vitritis (100%), anterior uveitis (70%), and scattered white deposit (80%). Pre-existing retinochoroidal scar was found in 1 (10%) patient. All patients received antiparasitic chemotherapy and systemic corticosteroid treatment, which resolved the presenting attack and recovered the visual acuity better than initial one in 9 patients and worse in 1. Optic atrophy, cataract, and retinal neovascularization were observed during the follow-up period and recurrence was detected in 3 eyes (30%) 6 to 20 months after the initial attack. In Korea, although rarely detected and reported, ocular toxoplasmosis needs more attention in clinical field of retinal diseases.

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Brief Communication

ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera: Hye-Jin Ahn, Sera Kim, Dae-Yong Kim, Ho-Woo Nam; Korean J Parasitol 2003;41(3):175-177.
Published online September 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.3.175

Abstract
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An ELISA was established to measure bovine IgG directed against the recombinant antigenic determinant of Nc-p43, a major surface antigen of Neospora caninum. In a previous study, two thirds of the C-terminal of the molecule was expressed as a 6 × His tagged protein (Ncp43P) for ELISA using 2/3 of the N-terminal of SAG1 from Toxoplasma gondii as a control (TgSAG1A). Among 852 cattle sera collected from stock farms scattered nation-wide, 103 sera (12.1%) were found to react with Ncp43P positively, but no positive reaction was observed with TgSAG1A. This study shows that Ncp43P could be available as an efficient antigen for the diagnosis of neosporosis in cattle. Furthermore, it together with TgSAG1A, could be useful for the differential diagnosis of N. caninum and T. gondii infections in other mammals.

Citations

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Revista Brasileira de Parasitologia Veterinária.2015; 24(2): 148. CrossRef
Diagnostic Potential of Anti-rNcp-43 Polyclonal Antibodies for the Detection of Neospora caninum
Gizele Lima de Sá, Diene de Borba Pacheco, Leonardo Garcia Monte, Francine Alves Sinnott, Marina Amaral Xavier, Caroline Rizzi, Sibele Borsuk, Maria Elisabeth Aires Berne, Renato Andreotti, Cláudia Pinho Hartleben
Current Microbiology.2014; 68(4): 472. CrossRef
Expression ofNeospora caninumNcSRS2 surface protein inPichia pastorisand its application for serodiagnosis ofNeosporainfection
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Pathogens and Global Health.2013; 107(3): 116. CrossRef
Development of an indirect ELISA-NcSRS2 for detection of Neospora caninum antibodies in cattle
Sibele Borsuk, Renato Andreotti, Fábio Pereira Leivas Leite, Luciano da Silva Pinto, Simone Simionatto, Claudia Pinho Hartleben, Marcelo Goetze, Leandra Marla Oshiro, Maria de Fátima Cepa Matos, Maria Elisabeth Aires Berne
Veterinary Parasitology.2011; 177(1-2): 33. CrossRef
Serodiagnosis of Neospora caninum infection in cattle using a recombinant tNcSRS2 protein-based ELISA
Jing Liu, Jinshu Yu, Ming Wang, Qun Liu, Wei Zhang, Chong Deng, Jun Ding
Veterinary Parasitology.2007; 143(3-4): 358. CrossRef
Epidemiology and Control of Neosporosis andNeospora caninum
J. P. Dubey, G. Schares, L. M. Ortega-Mora
Clinical Microbiology Reviews.2007; 20(2): 323. CrossRef
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Acta Parasitologica.2006; 51(1): 1. CrossRef
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Veterinary Parasitology.2006; 140(1-2): 1. CrossRef
Comparison of proteome and antigenic proteome between two Neospora caninum isolates
Yong-Seung Shin, Gee-Wook Shin, Young-Rim Kim, Eun-Young Lee, Hyang-Hee Yang, K.J. Palaksha, Hee-Jeong Youn, Jae-Hoon Kim, Dae-Yong Kim, A.E. Marsh, J. Lakritz, Tae-Sung Jung
Veterinary Parasitology.2005; 134(1-2): 41. CrossRef
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Veterinary Parasitology.2005; 131(3-4): 227. CrossRef
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PROTEOMICS.2004; 4(11): 3600. CrossRef
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Yong-seung Shin, Eung-goo Lee, Gee-wook Shin, Young-rim Kim, Eun-young Lee, Jae-hoon Kim, Hwan Jang, Dae-yong Kim, Yong-hwan Kim, Gon-sup Kim, Myung-deuk Suh, Tae-sung Jung
Clinical Proteomics.2004;[Epub] CrossRef

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Original Article

Detection of IgG antibody against Neospora caninum in cattle in Korea: Ji-Seon Bae, Dae-Yong Kim, Woo-Suk Hwang, Jae-Hoon Kim, Nam-Seok Lee, Ho-Woo Nam; Korean J Parasitol 2000;38(4):245-249.
Published online December 31, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.4.245

Abstract
PDF

A total of 492 cattle sera was screened by IgG-ELISA against Neospora caninum (Nc-1 strain and a Korean isolate, KBA-2) and Toxoplasma gondii. Out of 492, 113 sera (23.0%) reacted positively to either Nc-1 or KBA-2 strains of N. caninum. Among the 113 positive sera, 92 sera (81.4%) reacted with antigens of both strains, but 6 sera (5.3%) with Nc-1 and 15 sera (13.3%) with KBA-2 strain only. And with T. gondii antigen, 6 sera (1.2%) were positive but all reacted with N. caninum antigen also. Western blot revealed typical binding pattern according to ELISA values, such that high OD group reacted specifically to the major surface proteins including 43 kDa protein. Seroprevalence of 23.0% indicates that neosporosis seemed to be one of major causes of abortion in cattle. It is suggested here to establish more epidemiological researches nationwide systematically.

Citations

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Serological evaluation of Neospora caninum in pregnant women treated at referral center for prenatal screening in Mato Grosso do Sul, Brazil
Pâmella Oliveira Duarte, Bárbara Guimarães Csordas, Leandra Marla Oshiro, Leandro de Oliveira Souza Higa, Namor Pinheiro Zimmermann, Kauê Rodriguez Martins, Jacqueline Cavalcante Barros, Renato Andreotti
Revista Brasileira de Parasitologia Veterinária.2020;[Epub] CrossRef
Meta-analysis of the prevalence and risk factors associated with bovine neosporosis
Claudia Mello Ribeiro, Isabela Ribeiro Soares, Rodrigo Guerrero Mendes, Paula Andrea de Santis Bastos, Satie Katagiri, Renato Bacarin Zavilenski, Hudson Felipe Porto de Abreu, Vera Afreixo
Tropical Animal Health and Production.2019; 51(7): 1783. CrossRef
Seroprevalence of Neospora caninum infection in dairy cows in Northern provinces, Thailand
Tawin Inpankaew, Sathaporn Jittapalapong, Thomas Mitchell, Chainirun Sununta, Ikuo Igarashi, Xuenan Xuan
Acta Parasitologica.2014;[Epub] CrossRef
Risk factors of Neospora caninum infection in dogs and cats in dairy farms in Western Thailand
Pipat Arunvipas, Tawin Inpankaew, Sathaporn Jittapalapong
Tropical Animal Health and Production.2012; 44(5): 1117. CrossRef
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Thuy Nguyen, Se-Eun Choe, Jae-Won Byun, Hong-Bum Koh, Hee-Soo Lee, Seung-Won Kang
Acta Parasitologica.2012;[Epub] CrossRef
Seroprevalence of Neospora caninum Antibodies in Cattle in Veracruz, Mexico
D. Romero-Sal, Z. Garcia-Vaz, F. Montiel-Pa, T. Montiel-Pe, M. Aguilar-Do, L. Medina-Esp, C. Cruz-Vazqu
Journal of Animal and Veterinary Advances.2010; 9(10): 1445. CrossRef
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J. Liu, J.Z. Cai, W. Zhang, Q. Liu, D. Chen, J.P. Han, Q.R. Liu
Veterinary Parasitology.2008; 152(3-4): 330. CrossRef
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Jinhai Yu, Zhaofei Xia, Qun Liu, Jing Liu, Jun Ding, Wei Zhang
Veterinary Parasitology.2007; 143(1): 79. CrossRef
Epidemiology and Control of Neosporosis andNeospora caninum
J. P. Dubey, G. Schares, L. M. Ortega-Mora
Clinical Microbiology Reviews.2007; 20(2): 323. CrossRef
Diagnosis of bovine neosporosis
J.P. Dubey, G. Schares
Veterinary Parasitology.2006; 140(1-2): 1. CrossRef
ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera
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Seroprevalence of Neospora, Toxoplasma gondii and Sarcocystis neurona antibodies in horses from Jeju island, South Korea
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Veterinary Parasitology.2002; 106(3): 193. CrossRef
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Eui-Sun Son, Hye-Jin Ahn, Jae-Hoon Kim, Dae-Yong Kim, Ho-Woo Nam
The Korean Journal of Parasitology.2001; 39(3): 241. CrossRef