Trichomonas vaginalis is a flagellated protozoan parasite that causes vaginitis and cervicitis in women and asymptomatic urethritis and prostatitis in men. Mast cells have been reported to be predominant in vaginal smears and vaginal walls of patients infected with T. vaginalis. Mitogen-activated protein kinase (MAPK), activated by various stimuli, have been shown to regulate the transcriptional activity of various cytokine genes in mast cells. In this study, we investigated whether MAPK is involved in ROS generation and exocytotic degranulation in HMC-1 cells induced by T. vaginalis-derived secretory products (TvSP). We found that TvSP induces the activation of MAPK and NADPH oxidase in HMC-1 cells. Stimulation with TvSP induced phosphorylation of MAPK and p47phox in HMC-1 cells. Stimulation with TvSP also induced up-regulation of CD63, a marker for exocytosis, along the surfaces of human mast cells. Pretreatment with MAPK inhibitors strongly inhibited TvSP-induced ROS generation and exocytotic degranulation. Finally, our results suggest that TvSP induces intracellular ROS generation and exocytotic degranulation in HMC-1 via MAPK signaling.
Citations
Citations to this article as recorded by
Dynamin 2-mediated endocytosis of BLT1 is required for IL-8 production in HMC-1 cells induced by Trichomonas vaginalis-derived secretory products Young Ah Lee, Myeong Heon Shin Parasites, Hosts and Diseases.2024; 62(3): 281. CrossRef
Latent Upregulation of Nlrp3, Nlrc4 and Aim2 Differentiates between Asymptomatic and Symptomatic Trichomonas vaginalis Infection Sonal Yadav, Vivek Verma, Rakesh Singh Dhanda, Sumeeta Khurana, Manisha Yadav Immunological Investigations.2022; 51(5): 1127. CrossRef
Trichomonas vaginalis Induces SiHa Cell Apoptosis by NF-κB Inactivation via Reactive Oxygen Species Juan-Hua Quan, Byung-Hun Kang, Jung-Bo Yang, Yun-Ee Rhee, Heung-Tae Noh, In-Wook Choi, Guang-Ho Cha, Jae-Min Yuk, Young-Ha Lee BioMed Research International.2017; 2017: 1. CrossRef
SNAP23-Dependent Surface Translocation of Leukotriene B
4
(LTB
4
) Receptor 1 Is Essential for NOX2-Mediated Exocytotic Degranulation in Human Mast Cells Induced by Trichomonas vaginalis-Secrete Arim Min, Young Ah Lee, Kyeong Ah Kim, Jamel El-Benna, Myeong Heon Shin, Judith A. Appleton Infection and Immunity.2017;[Epub] CrossRef
Explore the variation of MMP3, JNK, p38 MAPKs, and autophagy at the early stage of osteoarthritis Jie Shi, Changjie Zhang, Zhongjie Yi, Chunna Lan IUBMB Life.2016; 68(4): 293. CrossRef
First
Prev
