A total of 492 cattle sera was screened by IgG-ELISA against Neospora caninum (Nc-1 strain and a Korean isolate, KBA-2) and Toxoplasma gondii. Out of 492, 113 sera (23.0%) reacted positively to either Nc-1 or KBA-2 strains of N. caninum. Among the 113 positive sera, 92 sera (81.4%) reacted with antigens of both strains, but 6 sera (5.3%) with Nc-1 and 15 sera (13.3%) with KBA-2 strain only. And with T. gondii antigen, 6 sera (1.2%) were positive but all reacted with N. caninum antigen also. Western blot revealed typical binding pattern according to ELISA values, such that high OD group reacted specifically to the major surface proteins including 43 kDa protein. Seroprevalence of 23.0% indicates that neosporosis seemed to be one of major causes of abortion in cattle. It is suggested here to establish more epidemiological researches nationwide systematically.
Citations
Citations to this article as recorded by
Serological evaluation of Neospora caninum in pregnant women treated at referral center for prenatal screening in Mato Grosso do Sul, Brazil Pâmella Oliveira Duarte, Bárbara Guimarães Csordas, Leandra Marla Oshiro, Leandro de Oliveira Souza Higa, Namor Pinheiro Zimmermann, Kauê Rodriguez Martins, Jacqueline Cavalcante Barros, Renato Andreotti Revista Brasileira de Parasitologia Veterinária.2020;[Epub] CrossRef
Meta-analysis of the prevalence and risk factors associated with bovine neosporosis Claudia Mello Ribeiro, Isabela Ribeiro Soares, Rodrigo Guerrero Mendes, Paula Andrea de Santis Bastos, Satie Katagiri, Renato Bacarin Zavilenski, Hudson Felipe Porto de Abreu, Vera Afreixo Tropical Animal Health and Production.2019; 51(7): 1783. CrossRef
Seroprevalence of Neospora caninum infection in dairy cows in Northern provinces, Thailand Tawin Inpankaew, Sathaporn Jittapalapong, Thomas Mitchell, Chainirun Sununta, Ikuo Igarashi, Xuenan Xuan Acta Parasitologica.2014;[Epub] CrossRef
Risk factors of Neospora caninum infection in dogs and cats in dairy farms in Western Thailand Pipat Arunvipas, Tawin Inpankaew, Sathaporn Jittapalapong Tropical Animal Health and Production.2012; 44(5): 1117. CrossRef
Seroprevalence of Toxoplasma gondii and Neospora caninum in dogs from Korea Thuy Nguyen, Se-Eun Choe, Jae-Won Byun, Hong-Bum Koh, Hee-Soo Lee, Seung-Won Kang Acta Parasitologica.2012;[Epub] CrossRef
Seroprevalence of Neospora caninum Antibodies in Cattle in Veracruz, Mexico D. Romero-Sal, Z. Garcia-Vaz, F. Montiel-Pa, T. Montiel-Pe, M. Aguilar-Do, L. Medina-Esp, C. Cruz-Vazqu Journal of Animal and Veterinary Advances.2010; 9(10): 1445. CrossRef
Seroepidemiology of Neospora caninum and Toxoplasma gondii infection in yaks (Bos grunniens) in Qinghai, China J. Liu, J.Z. Cai, W. Zhang, Q. Liu, D. Chen, J.P. Han, Q.R. Liu Veterinary Parasitology.2008; 152(3-4): 330. CrossRef
Seroepidemiology of Neospora caninum and Toxoplasma gondii in cattle and water buffaloes (Bubalus bubalis) in the People's Republic of China Jinhai Yu, Zhaofei Xia, Qun Liu, Jing Liu, Jun Ding, Wei Zhang Veterinary Parasitology.2007; 143(1): 79. CrossRef
Epidemiology and Control of Neosporosis andNeospora caninum J. P. Dubey, G. Schares, L. M. Ortega-Mora Clinical Microbiology Reviews.2007; 20(2): 323. CrossRef
Diagnosis of bovine neosporosis J.P. Dubey, G. Schares Veterinary Parasitology.2006; 140(1-2): 1. CrossRef
ELISA detection of IgG antibody against a recombinant major surface antigen (Nc-p43) fragment of Neospora caninum in bovine sera Hye-Jin Ahn, Sera Kim, Dae-Yong Kim, Ho-Woo Nam The Korean Journal of Parasitology.2003; 41(3): 175. CrossRef
Seroprevalence of Neospora, Toxoplasma gondii and Sarcocystis neurona antibodies in horses from Jeju island, South Korea G.D Gupta, J Lakritz, Jae-Hoon Kim, Dae-Yong Kim, Jin-Kap Kim, A.E Marsh Veterinary Parasitology.2002; 106(3): 193. CrossRef
Determination of antigenic domain in GST fused major surface protein (Nc-p43) of Neospora caninum Eui-Sun Son, Hye-Jin Ahn, Jae-Hoon Kim, Dae-Yong Kim, Ho-Woo Nam The Korean Journal of Parasitology.2001; 39(3): 241. CrossRef
First
Prev
