Sarcocystis spp. are common protozoan parasites infecting a wide range of intermediate hosts, including wild cervids. In this study, we investigated the occurrence and molecular characteristics of Sarcocystis infection in wild Korean roe deer (Capreolus pygargus tianschanicus) from Jeju Island, Korea. Sarcocysts were identified in the heart and skeletal muscle of 18 out of 40 examined animals (45.0%) based on histopathological examination. The cysts were oval to spherical in shape, measuring 42.41–340.29 μm in length and 31.62–129.76 μm in width, and were not associated with significant inflammatory responses. Molecular analysis targeting the 18S rRNA gene yielded 2 representative sequences, which showed 99.82% identity and high similarity to Sarcocystis linearis, as well as closely related species including S. taeniata and S. grueneri. Phylogenetic analysis placed these sequences within a clade of ungulate-associated Sarcocystis spp., although species-level resolution was not achieved due to the conserved nature of the marker. These findings provide baseline data on Sarcocystis infection in wild roe deer from Jeju Island and highlight the limitations of 18S rRNA-based identification for closely related Sarcocystis species.

Dirofilaria spp. are mosquito-borne filarial nematodes that primarily infect mammals. Here, we describe a filarial nematode infection in a female little egret (Egretta garzetta) from Jeju, Korea. Necropsy revealed multiple slender nematodes (4–6 cm) in the subcutaneous tissues of both legs. Histopathological examination demonstrated granulomatous inflammation with intralesional parasites. Morphologically, the nematodes exhibited a thick multilayered cuticle with prominent longitudinal ridges, consistent with members of the genus Dirofilaria. Molecular analysis based on 18S rRNA and mitochondrial c oxidase subunit I (cox1) gene sequences indicated that the parasite was most closely related to D. repens; however, the level of sequence divergence precluded definitive species-level identification. Phylogenetic analysis further demonstrated that the isolates clustered within the Dirofilaria clade and were clearly separated from avian-associated filarioid nematodes. This study provides molecular and histopathological evidence of a Dirofilaria sp. infection in an avian host and supports incidental infection under natural exposure conditions.

A stray female cat of unknown age, presenting bright red watery diarrhea, was submitted to the Animal and Plant Quarantine Agency for diagnosis. In the small intestines extracted from the necropsied cat, numerous white oval-shaped organisms were firmly embedded in the mucosa and there was thickening of intestinal wall. Histopathological analysis revealed severe necrotizing enteritis, together with atrophied intestinal villi, exfoliated enterocytes, and parasitic worms. Recovered worms were identified as Pharyngostomum cordatum by morphological observation and genetic analysis. Although P. cordatum is known to occur widely in Korea, this is the first clinical description of an infection by P. cordatum causing severe feline enteritis.