Parasites, Hosts and Diseases

"Juan-Hua Quan"

Original Articles

Evaluation of Protective Immune Response Induced by a DNA Vaccine Encoding GRA8 against Acute Toxoplasmosis in a Murine Model: Jia-Qi Chu, Shuai Huang, Wei Ye, Xuan-Yan Fan, Rui Huang, Shi-Cai Ye, Cai-Yuan Yu, Wei-Yun Wu, Yu Zhou, Wei Zhou, Young-Ha Lee, Juan-Hua Quan; Korean J Parasitol 2018;56(4):325-334.
Published online August 31, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.4.325

Abstract
PDF

Toxoplasma gondii is an apicomplexan zoonotic protozoan parasite that infects most species of warm-blooded animals, including humans. The heavy incidence and severe or lethal damage caused by T. gondii infection clearly indicate a need for the development of an effective vaccine. T. gondii GRA8 is a member of the dense granules protein family and is used as a marker of acute infection. In the present study, we evaluated the protective immunity induced by DNA vaccination based on a recombinant eukaryotic plasmid, pDsRed2-GRA8, against acute toxoplasmosis in mice. BALB/c mice were intramuscularly immunized with the pDsRed2-GRA8 plasmid and then challenged by infection with the highly virulent GFP-RH strain of T. gondii. The specific immune responses and protective efficacy against T. gondii of this vaccine were analyzed by measuring cytokine and serum antibody titers, splenocyte proliferation assays, and the survival times of mice after challenge. Our results showed that mice immunized with pDsRed2-GRA8 demonstrated specific humoral and cellular responses, induced higher IgG antibody titers with predominant IgG2a production; increased levels of IL-10, IL-12 (p70), IFN-γ, TNF-α, and splenocyte proliferation; and prolonged survival times compared to those of control mice. The present study showed that DNA immunization with pDsRed2-GRA8 induced humoral and cellular immune responses, and all immunized mice showed greater Th1-type immune responses and longer survival times than those of control mice. These results indicated that T. gondii GRA8 DNA immunization induces a partial protective effect against acute toxoplasmosis.

Citations

Citations to this article as recorded by

Toxoplasma gondii vaccine candidates: a concise review
Amirreza Javadi Mamaghani, Anwar Fathollahi, Zahra Arab-Mazar, Kobra kohansal, Matin Fathollahi, Adel Spotin, Homayoon Bashiri, Arezoo Bozorgomid
Irish Journal of Medical Science (1971 -).2023; 192(1): 231. CrossRef
Insight into the current Toxoplasma gondii DNA vaccine: a review article
Xirui Zhang, Hao Yuan, Yasser S. Mahmmod, Zipeng Yang, Mengpo Zhao, Yining Song, Shengjun Luo, Xiu-Xiang Zhang, Zi-Guo Yuan
Expert Review of Vaccines.2023; 22(1): 66. CrossRef
Co-Immunization with DNA Vaccines Expressing SABP1 and SAG1 Proteins Effectively Enhanced Mice Resistance to Toxoplasma gondii Acute Infection
Xiaoyu Sang, Xiang Li, Ran Chen, Ying Feng, Ting He, Xiaohan Zhang, Saeed El-Ashram, Ebtsam Al-Olayan, Na Yang
Vaccines.2023; 11(7): 1190. CrossRef
Evaluation of anti‐tick efficiency in rabbits induced by DNA vaccines encoding Haemaphysalis longicornis lipocalin homologue
Xiang‐Yuan Fan, Xiao‐Can Xu, Ya‐Xue Wu, Xiao‐Ya Liu, Feng Yang, Yong‐Hong Hu
Medical and Veterinary Entomology.2022; 36(4): 511. CrossRef
Review of DNA Vaccine Approaches Against the Parasite Toxoplasma gondii
Rosalie C. Warner, Ryan C. Chapman, Brianna N. Davis, Paul H. Davis
Journal of Parasitology.2021;[Epub] CrossRef
A systematic review on the role of GRA proteins of Toxoplasma gondii in host immunization
Fatemeh Rezaei, Mahdi Sharif, Shahabeddin Sarvi, Seyed Hossein Hejazi, Sargis Aghayan, Abdol Sattar Pagheh, Samira Dodangeh, Ahmad Daryani
Journal of Microbiological Methods.2019; 165: 105696. CrossRef

9,611 View
172 Download
8 Web of Science
Crossref

Modulated Gene Expression of Toxoplasma gondii Infected Retinal Pigment Epithelial Cell Line (ARPE-19) via PI3K/Akt or mTOR Signal Pathway: Wei Zhou, Juan-Hua Quan, Fei-Fei Gao, Hassan Ahmed Hassan Ahmed Ismail, Young-Ha Lee, Guang-Ho Cha; Korean J Parasitol 2018;56(2):135-145.
Published online April 30, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.2.135

Abstract
PDF

Due to the critical location and physiological activities of the retinal pigment epithelial (RPE) cell, it is constantly subjected to contact with various infectious agents and inflammatory mediators. However, little is known about the signaling events in RPE involved in Toxoplasma gondii infection and development. The aim of the study is to screen the host mRNA transcriptional change of 3 inflammation-related gene categories, PI3K/Akt pathway regulatory components, blood vessel development factors and ROS regulators, to prove that PI3K/Akt or mTOR signaling pathway play an essential role in regulating the selected inflammation-related genes. The selected genes include PH domain and leucine- richrepeat protein phosphatases (PHLPP), casein kinase2 (CK2), vascular endothelial growth factor (VEGF), pigment epithelium-derived factor (PEDF), glutamate-cysteine ligase (GCL), glutathione S-transferase (GST), and NAD(P)H: quinone oxidoreductase (NQO1). Using reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR), we found that T. gondii up-regulates PHLPP2, CK2β, VEGF, GCL, GST, and NQO1 gene expression levels, but down-regulates PHLPP1 and PEDF mRNA transcription levels. PI3K inhibition and mTOR inhibition by specific inhibitors showed that most of these host gene expression patterns were due to activation of PI3K/Akt or mTOR pathways with some exceptional cases. Taken together, our results reveal a new molecular mechanism of these gene expression change dependent on PI3K/Akt or mTOR pathways and highlight more systematical insight of how an intracellular T. gondii can manipulate host genes to avoid host defense.

Citations

Citations to this article as recorded by

PTEN regulation in virus-associated cancers
Shaian Tavakolian, Zahra Shokati Eshkiki, Abolfazl Akbari, Ebrahim Faghihloo, Seidamir Pasha Tabaeian
Pathology - Research and Practice.2025; 266: 155749. CrossRef
MjTX-II, a Lys49-PLA2 from Bothrops moojeni snake venom, restricts Toxoplasma gondii infection via ROS and VEGF regulation
Samuel Cota Teixeira, Thales Alves de Melo Fernandes, Guilherme de Souza, Alessandra Monteiro Rosini, Aryani Felixa Fajardo Martínez, Angelica Oliveira Gomes, Rosiane Nascimento Alves, Daiana Silva Lopes, Maria Vitoria da Silva, Emidio Beraldo-Neto, Patrí
Chemico-Biological Interactions.2025; 409: 111417. CrossRef
Echinococcus multilocularis protoscoleces enhance glycolysis to promote M2 Macrophages through PI3K/Akt/mTOR Signaling Pathway
Tao Zhang, Yaogang Zhang, Zihan Yang, Yuan Jiang, Li Sun, Dengliang Huang, Meiyuan Tian, Yinhong Shen, Jun Deng, Jing Hou, Yanyan Ma
Pathogens and Global Health.2023; 117(4): 409. CrossRef
The interplay between toxoplasmosis and host miRNAs: Mechanisms and consequences
Ahmed S. Doghish, Mohamed A. Ali, Mahmoud A. Elrebehy, Hend H. Mohamed, Reda Mansour, Aml Ghanem, Ahmed Hassan, Mohammed S. Elballal, Ola Elazazy, Ahmed E. Elesawy, Sherif S. Abdel Mageed, Yara A. Nassar, Osama A. Mohammed, Ahmed I. Abulsoud
Pathology - Research and Practice.2023; 250: 154790. CrossRef
Mammalian Target of Rapamycin Inhibitor Rapamycin Alleviates 7-Ketocholesterol Induced Inflammatory Responses and Vascular Endothelial Growth Factor Elevation by Regulating MAPK Pathway in Human Retinal Pigment Epithelium Cells
Lin Yang, Peng Yu, Mei Chen, Bo Lei
Journal of Ocular Pharmacology and Therapeutics.2022; 38(2): 189. CrossRef
The host mTOR pathway and parasitic diseases pathogenesis
Sajad Rashidi, Reza Mansouri, Mohammad Ali-Hassanzadeh, Zahra Mojtahedi, Reza Shafiei, Amir Savardashtaki, Nasrin Hamidizadeh, Mohammadreza Karimazar, Paul Nguewa, Raúl Manzano-Román
Parasitology Research.2021; 120(4): 1151. CrossRef
Upregulation of PEDF Predicts a Poor Prognosis and Promotes Esophageal Squamous Cell Carcinoma Progression by Modulating the MAPK/ERK Signaling Pathway
Zui Chen, Di Che, Xiaoqiong Gu, Jiamin Lin, Jing Deng, Ping Jiang, Kaixiong Xu, Banglao Xu, Ting Zhang
Frontiers in Oncology.2021;[Epub] CrossRef
Modulation of the mTOR pathway plays a central role in dendritic cell functions after Echinococcus granulosus antigen recognition
Christian Rodriguez Rodrigues, María Celeste Nicolao, Maia Chop, Natalia Plá, Mora Massaro, Julia Loos, Andrea C. Cumino
Scientific Reports.2021;[Epub] CrossRef
Ripasudil alleviated the inflammation of RPE cells by targeting the miR-136-5p/ROCK/NLRP3 pathway
Zhao Gao, Qiang Li, Yunda Zhang, Xiaohong Gao, Haiyan Li, Zhigang Yuan
BMC Ophthalmology.2020;[Epub] CrossRef
H3 relaxin protects against calcium oxalate crystal‐induced renal inflammatory pyroptosis
Jiannan Liu, Kelaier Yang, Yinshan Jin, Yadong Liu, Yaodong Chen, Xiaohui Zhang, Shiliang Yu, Erlin Song, Song Chen, Jingbo Zhang, Guanhua Jing, Ruihua An
Cell Proliferation.2020;[Epub] CrossRef
Dipenyleneiodonium Induces Growth Inhibition of Toxoplasma gondii through ROS Induction in ARPE-19 Cells
Pu Reum Sun, Fei Fei Gao, Hei Gwon Choi, Wei Zhou, Jae-Min Yuk, Jaeyul Kwon, Young-Ha Lee, Guang-Ho Cha
The Korean Journal of Parasitology.2019; 57(2): 83. CrossRef
Simultaneous Ribosome Profiling of Human Host Cells Infected with Toxoplasma gondii
Michael J. Holmes, Premal Shah, Ronald C. Wek, William J. Sullivan, Ira J. Blader
mSphere.2019;[Epub] CrossRef

11,140 View
182 Download
12 Web of Science
Crossref

Brief Communication

Fasciola hepatica: Infection Status of Freshwater Snails Collected from Gangwon-do (Province), Korea: Jae-Hyung Lee, Juan-Hua Quan, In-Wook Choi, Gab-Man Park, Guang-Ho Cha, Hyun-Ju Kim, Jae-Min Yuk, Young-Ha Lee; Korean J Parasitol 2017;55(1):95-98.
Published online February 28, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.1.95

Abstract
PDF

Fasciola hepatica is a trematode that causes zoonosis, mainly in cattle and sheep, and occasionally in humans. Few recent studies have determined the infection status of this fluke in Korea. In August 2015, we collected 402 samples of freshwater snails at Hoenggye-ri (upper stream) and Suha-ri (lower stream) of Song-cheon (stream) in Daegwalnyeong-myeon, Pyeongchang-gun in Gangwon-do (Province) near many large cattle or sheep farms. F. hepatica infection was determined using PCR on the nuclear ribosomal internal transcribed spacer 2 (ITS-2). Among the 402 samples, F. hepatica 1TS-2 marker was detected in 6 freshwater snails; thus, the overall prevalence in freshwater snails was 1.5%. The prevalence varied between collection areas, ranging from 0.0% at Hoenggye-ri to 2.9% at Suha-ri. However, F. gigantica ITS-2 was not detected in the 6 F. hepatica-positive samples by PCR. The nucleotide sequences of the 6 F. hepatica ITS-2 PCR-positive samples were 99.4% identical to the F. hepatica ITS-2 sequences in GenBank, whereas they were 98.4% similar to F. gigantica ITS-2 sequences. These results indicated that the prevalence of F. hepatica in snail intermediate hosts was 1.5% in Gangwon-do, Korea; however the prevalence varied between collection areas. These results may help us to understand F. hepatica infection status in natural environments.

Citations

Citations to this article as recorded by

Fasciola hepatica infection in Korean water deer (Hydropotes inermis argyropus)
Na-Hyeon Kim, Min-Gyeong Seo, Bumseok Kim, Yu Jeong Jeon, In Jung Jung, Il-Hwa Hong
Parasites, Hosts and Diseases.2025; 63(3): 243. CrossRef
Countering Zoonotic Diseases: Current Scenario and Advances in Diagnostics, Monitoring, Prophylaxis and Therapeutic Strategies
Saurabh Gupta, Rasanpreet Kaur, Jagdip Singh Sohal, Shoor Vir Singh, Kaushik Das, Manish Kumar Sharma, Jitendra Singh, Shalini Sharma, Kuldeep Dhama
Archives of Medical Research.2024; 55(6): 103037. CrossRef
The potential of snails as a source of food and feed
Akash Gupta, Prabhat Khanal
Journal of Agriculture and Food Research.2024; 18: 101330. CrossRef
Molluscicidal and cercaricidal effects of Persicaria senegalensis on Radix natalensis snails and their echinostome-shed cercariae in South Africa
MC Mathole, PH King
African Journal of Aquatic Science.2023; 48(1): 71. CrossRef
Epidemiology of Fasciola spp. in the intermediate host in China: A potential risk for fasciolosis transmission
Ming Pan, Shao-Yuan Bai, Tian-Kai Ji, Yi-Min Fan, Dan-Dan Liu, Yi Yang, Jian-Ping Tao, Si-Yang Huang
Acta Tropica.2022; 230: 106394. CrossRef
Economic losses, morpho-molecular identification, and identity of Fasciola species recovered from Egypt
Omima Ramadan Abdel‑Fatah, Waleed M. Arafa, Ahmed Anwar Wahba, Khaled Mohamed El‑Dakhly
Journal of Parasitic Diseases.2022; 46(4): 1036. CrossRef
Green vegetable juice as a potential source of human fascioliasis in Korea
Sungim Choi, Sunghee Park, Sooji Hong, Hyejoo Shin, Bong-Kwang Jung, Min Jae Kim
One Health.2022; 15: 100441. CrossRef
Human and Animal Fascioliasis: Origins and Worldwide Evolving Scenario
Santiago Mas-Coma, M. Adela Valero, M. Dolores Bargues
Clinical Microbiology Reviews.2022;[Epub] CrossRef
Living Liver Donor With Fasciola Hepatica: First Case Report in the Literature
Sami Akbulut, Tevfik Tolga Sahin, Mehmet Kolu, Burak Isik, Yasar Bayindir, Sezai Yilmaz
Experimental and Clinical Transplantation.2021; 19(3): 276. CrossRef
Trematode cercarial fauna obtained from the field-collected freshwater snails Lymnaea natalensis in Egypt
Amina M. Ibrahim, Amira Kamal Ahmed
Bulletin of the National Research Centre.2019;[Epub] CrossRef
Assessment of the molluscicidal impact of extracted chlorophyllin on some biochemical parameters in the nervous tissue and histological changes in Biomphalaria alexandrina and Lymnaea natalensis snails
Amina M. Ibrahim, Fayez A. Bakry
Invertebrate Neuroscience.2019;[Epub] CrossRef
A Case of Fascioliasis in A Wild Nutria, Myocastor coypus, in Republic of Korea
Hyo-Seok Kim, Joo-Yeon Kong, Jong-Hyun Kim, Seong-Chan Yeon, Il-Hwa Hong
The Korean Journal of Parasitology.2018; 56(4): 375. CrossRef
Purification and biochemical characterization of a 22-kDa stable cysteine- like protease from the excretory-secretory product of the liver fluke Fasciola hepatica by using conventional techniques
Ahmed Hemici, Roumaila Sabrina Benerbaiha, Dalila Bendjeddou
Journal of Chromatography B.2017; 1068-1069: 268. CrossRef

9,991 View
220 Download
12 Web of Science
Crossref

Original Article

Production of IL-1β and Inflammasome with Up-Regulated Expressions of NOD-Like Receptor Related Genes in Toxoplasma gondii-Infected THP-1 Macrophages: Jia-Qi Chu, Ge Shi, Yi-Ming Fan, In-Wook Choi, Guang-Ho Cha, Yu Zhou, Young-Ha Lee, Juan-Hua Quan; Korean J Parasitol 2016;54(6):711-717.
Published online December 31, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.6.711

Abstract
PDF

Toxoplasma gondii is an obligate intracellular parasite that stimulates production of high levels of proinflammatory cytokines, which are important for innate immunity. NLRs, i.e., nucleotide-binding oligomerization domain (NOD)-like receptors, play a crucial role as innate immune sensors and form multiprotein complexes called inflammasomes, which mediate caspase-1-dependent processing of pro-IL-1β. To elucidate the role of inflammasome components in T. gondii-infected THP-1 macrophages, we examined inflammasome-related gene expression and mechanisms of inflammasome-regulated cytokine IL-1β secretion. The results revealed a significant upregulation of IL-1β after T. gondii infection. T. gondii infection also upregulated the expression of inflammasome sensors, including NLRP1, NLRP3, NLRC4, NLRP6, NLRP8, NLRP13, AIM2, and NAIP, in a time-dependent manner. The infection also upregulated inflammasome adaptor protein ASC and caspase-1 mRNA levels. From this study, we newly found that T. gondii infection regulates NLRC4, NLRP6, NLRP8, NLRP13, AIM2, and neuronal apoptosis inhibitor protein (NAIP) gene expressions in THP-1 macrophages and that the role of the inflammasome-related genes may be critical for mediating the innate immune responses to T. gondii infection.

Citations

Citations to this article as recorded by

Investigating the possible role of toxoplasmosis and Interleukin-1β variants on the immune response in Egyptian diabetic patients
Asmaa Ibrahim, Nancy O. kamel, Fatma Rageh, Rasha Elgamal, Mohamed A. Sakr, Eman M. Osman, Samar s. Ahmed, Hend A. Yassin, Yasmine N. Kamel, Reham F. Othman, Manar Ezzelarab Ramadan
Microbes and Infection.2025; 27(7): 105559. CrossRef
Early responses of primary human and bovine monocytes, monocytic THP-1 cells and THP-1 cell-derived macrophages to vital Toxoplasma gondii tachyzoites
Dominik Hanke, Zahady D. Velásquez, Kathrin Büttner, Andreas Krueger, Ralf Ross, Andreas Hecker, Sybille Mazurek, Veronika Grau, Anja Taubert, Carlos Hermosilla, Katrin Richter, Iván Conejeros
Frontiers in Immunology.2025;[Epub] CrossRef
Unraveling the NLRP family: Structure, function, activation, critical influence on tumor progression, and potential as targets for cancer therapy
Xueqing Zhou, Yongguang Tao, Ying Shi
Cancer Letters.2024; 605: 217283. CrossRef
Role of inflammasomes in Toxoplasma and Plasmodium infections
Zhi-xin Wang, Wan-jun Jiao, Yong Yang, Hong-li Liu, Hai-long Wang
Parasites & Vectors.2024;[Epub] CrossRef
NLRP13 inflammasome complex is hypermethylated in familial Mediterranean fever and global methylation correlates with the disease severity
Feyzanur Yildirimtepe Caldiran, Koksal Deveci, Ercan Cacan
Annals of Human Genetics.2023; 87(3): 115. CrossRef
Overview of Apoptosis, Autophagy, and Inflammatory Processes in Toxoplasma gondii Infected Cells
Ehsan Ahmadpour, Farhad Babaie, Tohid Kazemi, Sirous Mehrani Moghaddam, Ata Moghimi, Ramin Hosseinzadeh, Veeranoot Nissapatorn, Abdol Sattar Pagheh
Pathogens.2023; 12(2): 253. CrossRef
P2X7 Receptor Modulation of the Gut Microbiota and the Inflammasome Determines the Severity of Toxoplasma gondii-Induced Ileitis
Aline Cristina Abreu Moreira-Souza, Hayandra Ferreira Nanini, Thuany Prado Rangel, Sthefani Rodrigues Batista da Silva, Beatriz Pêgo Damasceno, Beatriz Elias Ribeiro, Cynthia M. Cascabulho, Fabiano Thompson, Camille Leal, Patrícia Teixeira Santana, Siane
Biomedicines.2023; 11(2): 555. CrossRef
The role of NOD-like receptors in innate immunity
Cássio Luiz Coutinho Almeida-da-Silva, Luiz Eduardo Baggio Savio, Robson Coutinho-Silva, David M. Ojcius
Frontiers in Immunology.2023;[Epub] CrossRef
NLRP3 Plays a Key Role in Antihelminth Immunity in the Enteral and Parenteral Stages of Trichinella spiralis-Infected Mice
Tian-Xu Pan, Hai-Bin Huang, Hui-Nan Lu, Guang-Xun Zhao, Yu Quan, Jun-Yi Li, Ying Xue, Zhi-Yu Zhu, Yue Wang, Chun-Wei Shi, Nan Wang, Gui-Lian Yang, Chun-Feng Wang, De’Broski R. Herbert
Infection and Immunity.2023;[Epub] CrossRef
Toxoplasma gondii profilin induces NLRP3 activation and IL-1β production/secretion in THP-1 cells
Hossein Pazoki, Hamed Mirjalali, Maryam Niyyati, Seyed Javad Seyed Tabaei, Nariman Mosaffa, Shabnam Shahrokh, Hamid Asadzadeh Ahdaei, Andreas Kupz, Mohammad Reza Zali
Microbial Pathogenesis.2023; 180: 106120. CrossRef
Possible therapeutic targets for NLRP3 inflammasome-induced breast cancer
Xixi Wang, Junyi Lin, Zhe Wang, Zhi Li, Minghua Wang
Discover Oncology.2023;[Epub] CrossRef
Toxoplasma gondii Induces Pyroptosis in Human Placental Trophoblast and Amniotic Cells by Inducing ROS Production and Activation of Cathepsin B and NLRP1/NLRP3/NLRC4/AIM2 Inflammasome
Juan-Hua Quan, Fei Fei Gao, Tian-Zhong Ma, Wei Ye, Xiang Gao, Ming-Zhu Deng, Lan-Lan Yin, In-Wook Choi, Jae-Min Yuk, Guang-Ho Cha, Young-Ha Lee, Jia-Qi Chu
The American Journal of Pathology.2023; 193(12): 2047. CrossRef
Role of Microgliosis and NLRP3 Inflammasome in Parkinson’s Disease Pathogenesis and Therapy
Fillipe M. de Araújo, Lorena Cuenca-Bermejo, Emiliano Fernández-Villalba, Silvia L. Costa, Victor Diogenes A. Silva, Maria Trinidad Herrero
Cellular and Molecular Neurobiology.2022; 42(5): 1283. CrossRef
Latent Upregulation of Nlrp3, Nlrc4 and Aim2 Differentiates between Asymptomatic and Symptomatic Trichomonas vaginalis Infection
Sonal Yadav, Vivek Verma, Rakesh Singh Dhanda, Sumeeta Khurana, Manisha Yadav
Immunological Investigations.2022; 51(5): 1127. CrossRef
P. aeruginosa biofilm activates the NLRP3 inflammasomes in vitro
Qi Tan, Qing Ai, Yu He, Fang Li, Jialin Yu
Microbial Pathogenesis.2022; 164: 105379. CrossRef
In silico analysis of expression and DNA methylation profiles of NLRP13 inflammasome in tumor cells
Feyzanur Yildirimtepe Caldiran, Caglar Berkel, Koksal Deveci, Ercan Cacan
Human Gene.2022; 33: 201067. CrossRef
The strategies of NLRP3 inflammasome to combat Toxoplasma gondii
Chanjin Yoon, Yu Seong Ham, Woo Jin Gil, Chul-Su Yang
Frontiers in Immunology.2022;[Epub] CrossRef
Expression profiles of NOD-like receptors and regulation of NLRP3 inflammasome activation in Toxoplasma gondii-infected human small intestinal epithelial cells
Jia-Qi Chu, Fei Fei Gao, Weiyun Wu, Chunchao Li, Zhaobin Pan, Jinhui Sun, Hao Wang, Cong Huang, Sang Hyuk Lee, Juan-Hua Quan, Young-Ha Lee
Parasites & Vectors.2021;[Epub] CrossRef
RIPK3 Facilitates Host Resistance to Oral Toxoplasma gondii Infection
Patrick W. Cervantes, Bruno Martorelli Di Genova, Billy Joel Erazo Flores, Laura J. Knoll, Jeroen P. J. Saeij
Infection and Immunity.2021;[Epub] CrossRef
Soluble total antigen derived from Toxoplasma gondii tachyzoites increased the expression levels of NLRP1, NLRP3, NLRC4, AIM2, and the release of mature form of IL1β, but downregulated the expression of IL1β and IL18 genes in THP-1cell line
Hossein Pazoki, Hanieh Mohammad Rahimi, Hamed Mirjalali, Maryam Niyyati, Nariman Mosaffa, Seyed Javad Seyed Tabaei, Shabnam Shahrokh, Hamid Asadzadeh Aghdaei, Mohammad Reza Zali
Microbial Pathogenesis.2021; 158: 105072. CrossRef
Host inflammatory responses to intracellular invaders: Review study
Ramesh Chandra Rai
Life Sciences.2020; 240: 117084. CrossRef
Treatment of mice with S4B6 IL-2 complex prevents lethal toxoplasmosis via IL-12- and IL-18-dependent interferon-gamma production by non-CD4 immune cells
Andreas Kupz, Saparna Pai, Paul R. Giacomin, Jennifer A. Whan, Robert A. Walker, Pierre-Mehdi Hammoudi, Nicholas C. Smith, Catherine M. Miller
Scientific Reports.2020;[Epub] CrossRef
Neospora caninum infection induces an isolate virulence-dependent pro-inflammatory gene expression profile in bovine monocyte-derived macrophages
Marta García-Sánchez, Laura Jiménez-Pelayo, Pilar Horcajo, Esther Collantes-Fernández, Luis Miguel Ortega-Mora, Javier Regidor-Cerrillo
Parasites & Vectors.2020;[Epub] CrossRef
A Comparison of Transcriptional Diversity of Swine Macrophages Infected With TgHB1 Strain of Toxoplasma gondii Isolated in China
Yongle Song, Lindong Song, Xiaoting Wan, Bang Shen, Rui Fang, Min Hu, Junlong Zhao, Yanqin Zhou
Frontiers in Cellular and Infection Microbiology.2020;[Epub] CrossRef
Cytosolic Recognition of Microbes and Pathogens: Inflammasomes in Action
Jenni A. Hayward, Anukriti Mathur, Chinh Ngo, Si Ming Man
Microbiology and Molecular Biology Reviews.2018;[Epub] CrossRef
P2X7 receptor mediates NLRP3-dependent IL-1β secretion and parasite proliferation in Toxoplasma gondii-infected human small intestinal epithelial cells
Juan-Hua Quan, Rui Huang, Zhuang Wang, Shuai Huang, In-Wook Choi, Yu Zhou, Young-Ha Lee, Jia-Qi Chu
Parasites & Vectors.2018;[Epub] CrossRef

12,704 View
294 Download
27 Web of Science
Crossref

Brief Communication

Monitoring of Fasciola Species Contamination in Water Dropwort by COX1 Mitochondrial and ITS-2 rDNA Sequencing Analysis: In-Wook Choi, Hwang-Yong Kim, Juan-Hua Quan, Jae-Gee Ryu, Rubing Sun, Young-Ha Lee; Korean J Parasitol 2015;53(5):641-645.
Published online October 29, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.5.641

Abstract
PDF

Fascioliasis, a food-borne trematode zoonosis, is a disease primarily in cattle and sheep and occasionally in humans. Water dropwort (Oenanthe javanica), an aquatic perennial herb, is a common second intermediate host of Fasciola, and the fresh stems and leaves are widely used as a seasoning in the Korean diet. However, no information regarding Fasciola species contamination in water dropwort is available. Here, we collected 500 samples of water dropwort in 3 areas in Korea during February and March 2015, and the water dropwort contamination of Fasciola species was monitored by DNA sequencing analysis of the Fasciola hepatica and Fasciola gigantica specific mitochondrial cytochrome c oxidase subunit 1 (cox1) and nuclear ribosomal internal transcribed spacer 2 (ITS-2). Among the 500 samples assessed, the presence of F. hepatica cox1 and 1TS-2 markers were detected in 2 samples, and F. hepatica contamination was confirmed by sequencing analysis. The nucleotide sequences of cox1 PCR products from the 2 F. hepatica-contaminated samples were 96.5% identical to the F. hepatica cox1 sequences in GenBank, whereas F. gigantica cox1 sequences were 46.8% similar with the sequence detected from the cox1 positive samples. However, F. gigantica cox1 and ITS-2 markers were not detected by PCR in the 500 samples of water dropwort. Collectively, in this survey of the water dropwort contamination with Fasciola species, very low prevalence of F. hepatica contamination was detected in the samples.

Citations

Citations to this article as recorded by

Isolation and molecular identification of liver fluke cercariae in freshwater snails of Chaharmahal and Bakhtiari province, Iran
Bijan Hosseinpour Aghaei, Nadia Taiefi Nasrabadi, Yaser Pirali Kheirabadi, Seyed Shapoor Reza Shojaei
Molluscan Research.2024; 44(1): 84. CrossRef
Morphological and molecular identification of lymnaeid snail and trematodes cercariae in different water bodies in Perak, Malaysia
Nazir Ahmad Tookhy, Nur Mahiza Md Isa, Rozaihan Mansor, Yasmin Abd Rahaman, Nur Indah Ahmad, Dung Thi Bui, Lokman Hakim Idris, Noor Hazfalinda Hamzah, Norhadila Zulkifli
Parasitology Research.2023; 122(7): 1475. CrossRef
Green vegetable juice as a potential source of human fascioliasis in Korea
Sungim Choi, Sunghee Park, Sooji Hong, Hyejoo Shin, Bong-Kwang Jung, Min Jae Kim
One Health.2022; 15: 100441. CrossRef
Phylogenetic Characteristics of Fasciola hepatica Isolated from a Korean Patient
Mi Jin Jeong, Jae Kyun Park, Hak Sun Yu
The Korean Journal of Parasitology.2022; 60(5): 367. CrossRef
A Descriptive Study of Human Fascioliasis in Qaemshahr, Mazandaran Province, Iran: Its Prevalence and Risk Factors
Lotfollah Davoodi, Azadeh Mizani, Roya Najafi-Vosough, Saeed Hosseini Teshnizi, afsane amouei, Mousa Motavallihaghi, Hamideh Izadyar, Fateme Amuei, Sara Pourhaghighi, Seyed Reza Mirbadie, Eissa Soleymani
Archives of Clinical Infectious Diseases.2022;[Epub] CrossRef
A Review ofOenanthe javanica(Blume) DC. as Traditional Medicinal Plant and Its Therapeutic Potential
Chuan-li Lu, Xiu-fen Li
Evidence-Based Complementary and Alternative Medicine.2019; 2019: 1. CrossRef
Human fascioliasis infection sources, their diversity, incidence factors, analytical methods and prevention measures
S. Mas-Coma, M. D. Bargues, M. A. Valero
Parasitology.2018; 145(13): 1665. CrossRef

9,876 View
107 Download
8 Web of Science
Crossref

Original Articles

Involvement of PI3K/AKT and MAPK Pathways for TNF-α Production in SiHa Cervical Mucosal Epithelial Cells Infected with Trichomonas vaginalis: Jung-Bo Yang, Juan-Hua Quan, Ye-Eun Kim, Yun-Ee Rhee, Byung-Hyun Kang, In-Wook Choi, Guang-Ho Cha, Jae-Min Yuk, Young-Ha Lee; Korean J Parasitol 2015;53(4):371-377.
Published online August 25, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.4.371

Abstract
PDF

Trichomonas vaginalis induces proinflammation in cervicovaginal mucosal epithelium. To investigate the signaling pathways in TNF-α production in cervical mucosal epithelium after T. vaginalis infection, the phosphorylation of PI3K/AKT and MAPK pathways were evaluated in T. vaginalis-infected SiHa cells in the presence and absence of specific inhibitors. T. vaginalis increased TNF-α production in SiHa cells, in a parasite burden-dependent and incubation time-dependent manner. In T. vaginalis-infected SiHa cells, AKT, ERK1/2, p38 MAPK, and JNK were phosphorylated from 1 hr after infection; however, the phosphorylation patterns were different from each other. After pretreatment with inhibitors of the PI3K/AKT and MAPK pathways, TNF-α production was significantly decreased compared to the control; however, TNF-α reduction patterns were different depending on the type of PI3K/MAPK inhibitors. TNF-α production was reduced in a dose-dependent manner by treatment with wortmannin and PD98059, whereas it was increased by SP600125. These data suggested that PI3K/AKT and MAPK signaling pathways are important in regulation of TNF-α production in cervical mucosal epithelial SiHa cells. However, activation patterns of each pathway were different from the types of PI3K/MAPK pathways.

Citations

Citations to this article as recorded by

Exploring the therapeutic potential and in vitro validation of baicalin for the treatment of triple-negative breast cancer
Yuan Ma, Ying Pan, Qiancheng Zhao, Chongheng Zhang, Haitao He, Lihua Pan, Jianling Jia, Aiping Shi, Yiming Yang, Wenfeng Zhang
Frontiers in Pharmacology.2025;[Epub] CrossRef
PI3K/AKT signaling in parasites and parasite diseases: Role and therapeutic potential
Lujun Yan, Yating Li, Xing Yang, Rui Li, Chunyin Zhu, Xuedong He, Xiaoliang Jin, Guanghui Zheng, Naunain Mehmood, William C. Cho, Shijun Bao, Houhui Song, Yadong Zheng
Virulence.2025;[Epub] CrossRef
Interactions between bacterial vaginosis-associated microbiota and Trichomonas vaginalis modulate parasite-induced pathogenicity and host immune responses
Shu-Fang Chiu, Ching-Yun Huang, Chien-Yung Chen, Wei-Jane Hsu, Yuan-Ming Yeh, Ya-Wen Shih, Lichieh Julie Chu, Wei-Ning Lin, Kuo-Yang Huang
Parasites & Vectors.2025;[Epub] CrossRef
Therapeutic effects of coptisine derivative EHLJ7 on colorectal cancer by inhibiting PI3K/AKT pathway
ChangWei Chai, XiaoNan Tang, XiaoQian Chi, Xiang Li, HaiJing Zhang, LianQiu Wu
Cellular Signalling.2024; 116: 111053. CrossRef
Inflammatory responses during trichomoniasis: The role of Toll‐like receptors and inflammasomes
Abdollah Jafarzadeh, Maryam Nemati, Ehsan Salarkia, Sonal Yadav, Najmeh Aminizadeh, Sara Jafarzadeh, Manisha Yadav
Parasite Immunology.2023;[Epub] CrossRef
Chemokine CXCL10 regulates pain behaviors via PI3K-AKT signaling pathway in mice
Yan Fang, Xiaoling Peng, Huilian Bu, Xiaoqian Jia, Feng Gao, Cheng Liu
Neuropeptides.2022; 93: 102243. CrossRef
The role of TNF-α induced protein 1 in the activation of pro-apoptotic proteins
Xiaoren Tang, Thanarut Tangkham, Bushra Aljahdali, Sean Lee, Mingfang Su, Serge Dibart
Human Cell.2021; 34(4): 1123. CrossRef
Prevention of EHLJ7 on Colitis-Associated Colorectal Cancer Tumorigenesis Through Regulating PI3K/AKT Pathway
XiaoNan Tang, ChangWei Chai, Ying Guan, Xiang Li, AnJun Deng, HaiLin Qin, HaiJing Zhang, Lianqiu Wu
SSRN Electronic Journal .2021;[Epub] CrossRef
LncRNA MNX1‐AS1 promotes the progression of cervical cancer through activating MAPK pathway
Xiang Liu, Qian Yang, Jinyu Yan, Xiahui Zhang, Meiyun Zheng
Journal of Cellular Biochemistry.2019; 120(3): 4268. CrossRef
Trichomonas vaginalis Induces Production of Proinflammatory Cytokines in Mouse Macrophages Through Activation of MAPK and NF-κB Pathways Partially Mediated by TLR2
Ling Li, Xin Li, Pengtao Gong, Xichen Zhang, Zhengtao Yang, Ju Yang, Jianhua Li
Frontiers in Microbiology.2018;[Epub] CrossRef
Mapping out p38MAPK
Elizabeth A. Bonney
American Journal of Reproductive Immunology.2017;[Epub] CrossRef
C5a Induces the Synthesis of IL-6 and TNF-α in Rat Glomerular Mesangial Cells through MAPK Signaling Pathways
Mingde Ji, Yanlai Lu, Chenhui Zhao, Wenxing Gao, Fengxia He, Jing Zhang, Dan Zhao, Wen Qiu, Yingwei Wang, Hiroyasu Nakano
PLOS ONE.2016; 11(9): e0161867. CrossRef

9,271 View
92 Download
12 Web of Science
Crossref

Genetic Diversity of Schistosoma haematobium Eggs Isolated from Human Urine in Sudan: Juan-Hua Quan, In-Wook Choi, Hassan Ahmed Hassan Ahmed Ismail, Abdoelohab Saed Mohamed, Hoo-Gn Jeong, Jin-Su Lee, Sung-Tae Hong, Tai-Soon Yong, Guang-Ho Cha, Young-Ha Lee; Korean J Parasitol 2015;53(3):271-277.
Published online June 30, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.3.271

Abstract
PDF

The genetic diversity of Schistosoma haematobium remains largely unstudied in comparison to that of Schistosoma mansoni. To characterize the extent of genetic diversity in S. haematobium among its definitive host (humans), we collected S. haematobium eggs from the urine of 73 infected schoolchildren at 5 primary schools in White Nile State, Sudan, and then performed a randomly amplified polymorphic DNA marker ITS2 by PCR-RFLP analysis. Among 73 S. haematobium egg-positive cases, 13 were selected based on the presence of the S. haematobium satellite markers A4 and B2 in their genomic DNA, and used for RFLP analysis. The 13 samples were subjected to an RFLP analysis of the S. haematobium ITS2 region; however, there was no variation in size among the fragments. Compared to the ITS2 sequences obtained for S. haematobium from Kenya, the nucleotide sequences of the ITS2 regions of S. haematobium from 4 areas in Sudan were consistent with those from Kenya (> 99%). In this study, we demonstrate for the first time that most of the S. haematobium population in Sudan consists of a pan-African S. haematobium genotype; however, we also report the discovery of Kenyan strain inflow into White Nile, Sudan.

Citations

Citations to this article as recorded by

Analysis of DNA cox1 barcoding revealed novel haplotype in Schistosoma haematobium isolated from Western Sudan
Ishraga Adam Elzain, Abeer Babiker Idris, Abdul Aziz Karim, Nagla Mohamed Ahmed, Salaheldein G. Elzaki, Semih Yılmaz, Mohamed A. Hassan, Hamid Suliman Abdalla
Scientific Reports.2025;[Epub] CrossRef
Molecular Detection of Urogenital Schistosomiasis in Community Level in Semi-Rural Areas in South-East Gabon
Lady Charlène Kouna, Sandrine Lydie Oyegue-Liabagui, Chenis Nick Atiga, Chérone Nancy Mbani Mpega Ntigui, Roméo Karl Imboumy-Limoukou, Jean Claude Biteghe BI Essone, Steede Seinnat Ontoua, Diamella Nancy Moukodoum, Alain Prince Okouga, Jean Bernard Lekana
Diagnostics.2025; 15(9): 1052. CrossRef
Molecular diagnosis of urogenital schistosomiasis in pre-school children, school-aged children and women of reproductive age at community level in central Senegal
Doudou Sow, Khadime Sylla, Ndeye Marème Dieng, Bruno Senghor, Papa Mouhamadou Gaye, Cheikh B. Fall, Ndiaw Goumballa, Aldiouma Diallo, Jean Louis A. Ndiaye, Philippe Parola, Cheikh Sokhna, Souleymane Doucouré, Babacar Faye
Parasites & Vectors.2023;[Epub] CrossRef
Population genetic structure of Schistosoma haematobium and Schistosoma haematobium × Schistosoma bovis hybrids among school-aged children in Côte d’Ivoire
Etienne K. Angora, Alexane Vangraefschepe, Jean-François Allienne, Hervé Menan, Jean T. Coulibaly, Aboulaye Meïté, Giovanna Raso, Mirko S. Winkler, William Yavo, André O. Touré, Eliézer K. N’Goran, Jakob Zinsstag, Jürg Utzinger, Oliver Balmer, Jérôme Bois
Parasite.2022; 29: 23. CrossRef
Genetic Diversity of Schistosoma haematobium in Sub-Saharan Africa: A Systematic Review
Rabecca Tembo, Panji Nkhoma, Mildred Zulu, Florence Mwaba, John Yabe, Hikabasa Halwiindi, Moses Kamwela, King S Nalubamba, Chummy S Sikasunge, Andrew M Phri
University of Zambia Journal of Agricultural and Biomedical Sciences.2022;[Epub] CrossRef
Detection of Schistosoma DNA in genital specimens and urine: A comparison between five female African study populations originating from S. haematobium and/or S. mansoni endemic areas
P. Pillay, J.A. Downs, J.M. Changalucha, E.A.T. Brienen, C.E. Ramarokoto, P.D.C. Leutscher, B.J. Vennervald, M. Taylor, E.F. Kjetland, L. Van Lieshout
Acta Tropica.2020; 204: 105363. CrossRef

11,745 View
207 Download
8 Web of Science
Crossref

Fasciola hepatica in Snails Collected from Water-Dropwort Fields using PCR: Hwang-Yong Kim, In-Wook Choi, Yeon-Rok Kim, Juan-Hua Quan, Hassan Ahmed Hassan Ahmed Ismail, Guang-Ho Cha, Sung-Jong Hong, Young-Ha Lee; Korean J Parasitol 2014;52(6):645-652.
Published online December 23, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.6.645

Abstract
PDF

Fasciola hepatica is a trematode that causes zoonosis mainly in cattle and sheep and occasionally in humans. Fascioliasis has been reported in Korea; however, determining F. hepatica infection in snails has not been done recently. Thus, using PCR, we evaluated the prevalence of F. hepatica infection in snails at 4 large water-dropwort fields. Among 349 examined snails, F. hepatica-specific internal transcribed space 1 (ITS-1) and/or ITS-2 markers were detected in 12 snails and confirmed using sequence analysis. Morphologically, 213 of 349 collected snails were dextral shelled, which is the same aperture as the lymnaeid snail, the vectorial host for F. hepatica. Among the 12 F. hepatica-infected snails, 6 were known first intermediate hosts in Korea (Lymnaea viridis and L. ollula) and the remaining 6 (Lymnaea sp.) were potentially a new first intermediate host in Korea. It has been shown that the overall prevalence of the snails contaminated with F. hepatica in water-dropwort fields was 3.4%; however, the prevalence varied among the fields. This is the first study to estimate the prevalence of F. hepatica infection using the vectorial capacity of the snails in Korea.

Citations

Citations to this article as recorded by

Distribution and Fasciola infection rates of Lymnaea snails and cattle in high-salinity areas of Mekong Delta, Vietnam
Dang Thi LOAN, Lam Thanh NGUYEN, Tran Ngoc BICH, Nguyen Thuy Y VI, Yasunobu MATSUMOTO
Journal of Veterinary Medical Science.2025; 87(3): 291. CrossRef
Susceptibility of lymnaeid snails to Fasciola hepatica and Fasciola gigantica (Digenea: Fasciolidae): a systematic review and meta-analysis
Philile Ignecious Ngcamphalala, Ignore Nyagura, Mokgadi Pulane Malatji, Samson Mukaratirwa
PeerJ.2025; 13: e18976. CrossRef
Green vegetable juice as a potential source of human fascioliasis in Korea
Sungim Choi, Sunghee Park, Sooji Hong, Hyejoo Shin, Bong-Kwang Jung, Min Jae Kim
One Health.2022; 15: 100441. CrossRef
Snail-borne parasitic diseases: an update on global epidemiological distribution, transmission interruption and control methods
Xiao-Ting Lu, Qiu-Yun Gu, Yanin Limpanont, Lan-Gui Song, Zhong-Dao Wu, Kamolnetr Okanurak, Zhi-Yue Lv
Infectious Diseases of Poverty.2018;[Epub] CrossRef
Morphological Characterization of Emerging Cercariae among Lymnaeid Snails from Barangay Cawongan, Padre Garcia, Batangas, Philippines
Gregorio L. Martin I, Esperanza C. Cabrera
Journal of Parasitology Research.2018; 2018: 1. CrossRef
A Case of Fascioliasis in A Wild Nutria, Myocastor coypus, in Republic of Korea
Hyo-Seok Kim, Joo-Yeon Kong, Jong-Hyun Kim, Seong-Chan Yeon, Il-Hwa Hong
The Korean Journal of Parasitology.2018; 56(4): 375. CrossRef
Monitoring of Fasciola Species Contamination in Water Dropwort by COX1 Mitochondrial and ITS-2 rDNA Sequencing Analysis
In-Wook Choi, Hwang-Yong Kim, Juan-Hua Quan, Jae-Gee Ryu, Rubing Sun, Young-Ha Lee
The Korean Journal of Parasitology.2015; 53(5): 641. CrossRef
Ectopic Human <i>Fasciola hepatica</i> Infection by an Adult Worm in the Mesocolon
Ah Jin Kim, Chang Hwan Choi, Sun Keun Choi, Yong Woon Shin, Yun-Kyu Park, Lucia Kim, Suk Jin Choi, Jee Young Han, Joon Mee Kim, Young Chae Chu, In Suh Park
The Korean Journal of Parasitology.2015; 53(6): 725. CrossRef

11,796 View
139 Download
9 Web of Science
Crossref

Trichomonas vaginalis Metalloproteinase Induces mTOR Cleavage of SiHa Cells: Juan-Hua Quan, In-Wook Choi, Jung-Bo Yang, Wei Zhou, Guang-Ho Cha, Yu Zhou, Jae-Sook Ryu, Young-Ha Lee; Korean J Parasitol 2014;52(6):595-603.
Published online December 23, 2014; DOI: https://doi.org/10.3347/kjp.2014.52.6.595

Abstract
PDF

Trichomonas vaginalis secretes a number of proteases which are suspected to be the cause of pathogenesis; however, little is understood how they manipulate host cells. The mammalian target of rapamycin (mTOR) regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. We detected various types of metalloproteinases including GP63 protein from T. vaginalis trophozoites, and T. vaginalis GP63 metalloproteinase was confirmed by sequencing and western blot. When SiHa cells were stimulated with live T. vaginalis, T. vaginalis excretory-secretory products (ESP) or T. vaginalis lysate, live T. vaginalis and T. vaginalis ESP induced the mTOR cleavage in both time- and parasite load-dependent manner, but T. vaginalis lysate did not. Pretreatment of T. vaginalis with a metalloproteinase inhibitor, 1,10-phenanthroline, completely disappeared the mTOR cleavage in SiHa cells. Collectively, T. vaginalis metallopeptidase induces host cell mTOR cleavage, which may be related to survival of the parasite.

Citations

Citations to this article as recorded by

Chromatin accessibility and gene expression in the parasite Trichomonas vaginalis
Agustina Prat, Daniela Muñoz, Ayelen Lizarraga, Julieta Seifert-Gorzycki, Estefania Sanchez-Vazquez, Patricia J. Johnson, Pablo H. Strobl-Mazzulla, Natalia de Miguel
BMC Infectious Diseases.2025;[Epub] CrossRef
Metallopeptidases as Key Virulence Attributes of Clinically Relevant Protozoa: New Discoveries, Perspectives, and Frontiers of Knowledge
Graziela Vargas Rigo, Fernanda Gomes Cardoso, Giulia Bongiorni Galego, Deisiane Fernanda da Rosa, André Luis Souza dos Santos, Tiana Tasca
Current Protein & Peptide Science.2023; 24(4): 307. CrossRef
VPS32, a member of the ESCRT complex, modulates adherence to host cells in the parasite Trichomonas vaginalis by affecting biogenesis and cargo sorting of released extracellular vesicles
Nehuén Salas, Veronica M. Coceres, Tuanne dos Santos Melo, Antonio Pereira-Neves, Vanina G. Maguire, Tania M. Rodriguez, Bruna Sabatke, Marcel I. Ramirez, Jihui Sha, James A. Wohlschlegel, Natalia de Miguel
Cellular and Molecular Life Sciences.2022;[Epub] CrossRef
Adherent Bacteria and Parasiticidal Secretion Products of Human Cervicovaginal Microbiota-Associated Lactobacillus gasseri Confer Non-Identical Cell Protection against Trichomonas vaginalis-Induced Cell Detachment
Bénédicte Pradines, Séverine Domenichini, Vanessa Lievin-Le Moal
Pharmaceuticals.2022; 15(11): 1350. CrossRef
The 50 kDa metalloproteinase TvMP50 is a zinc-mediated Trichomonas vaginalis virulence factor
Jonathan Puente-Rivera, José Luis Villalpando, Alma Villalobos-Osnaya, Laura Isabel Vázquez-Carrillo, Gloria León-Ávila, María Dolores Ponce-Regalado, César López-Camarillo, Jose Miguel Elizalde-Contreras, Eliel Ruiz-May, Rossana Arroyo, María Elizbeth Al
Molecular and Biochemical Parasitology.2017; 217: 32. CrossRef
Regulation of exosomes released from normal ovarian epithelial cells and ovarian cancer cells
Wei Zhang, Jiaxin Yang, Dongyan Cao, Yan You, Keng Shen, Peng Peng
Tumor Biology.2016; 37(12): 15763. CrossRef
Involvement of PI3K/AKT and MAPK Pathways for TNF-α Production in SiHa Cervical Mucosal Epithelial Cells Infected with <i>Trichomonas vaginalis</i>
Jung-Bo Yang, Juan-Hua Quan, Ye-Eun Kim, Yun-Ee Rhee, Byung-Hyun Kang, In-Wook Choi, Guang-Ho Cha, Jae-Min Yuk, Young-Ha Lee
The Korean Journal of Parasitology.2015; 53(4): 371. CrossRef

11,447 View
103 Download
7 Web of Science
Crossref

Kinetics of IL-23 and IL-12 Secretion in Response to Toxoplasma gondii Antigens from THP-1 Monocytic Cells: Juan-Hua Quan, Wei Zhou, Guang-Ho Cha, In-Wook Choi, Dae-Whan Shin, Young-Ha Lee; Korean J Parasitol 2013;51(1):85-92.
Published online February 18, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.1.85

Abstract
PDF

IL-23 and IL-12 are structurally similar and critical for the generation of efficient cellular immune responses. Toxoplasma gondii induces a strong cell-mediated immune response. However, little is known about IL-23 secretion profiles in T. gondii-infected immune cells in connection with IL-12. We compared the patterns of IL-23 and IL-12 production by THP-1 human monocytic cells in response to stimulation with live or heat-killed T. gondii tachyzoites, or with equivalent quantities of either T. gondii excretory/secretory proteins (ESP) or soluble tachyzoite antigen (STAg). IL-23 and IL-12 were significantly increased from 6 hr after stimulation with T. gondii antigens, and their secretions were increased with parasite dose-dependent manner. IL-23 concentrations were significantly higher than those of IL-12 at the same multiplicity of infection. IL-23 secretion induced by live parasites was significantly higher than that by heat-killed parasites, ESP, or STAg, whereas IL-12 secretion by live parasite was similar to those of ESP or STAg. However, the lowest levels of both cytokines were at stimulation with heat-killed parasites. These data indicate that IL-23 secretion patterns by stimulation with various kinds of T. gondii antigens at THP-1 monocytic cells are similar to those of IL-12, even though the levels of IL-23 induction were significantly higher than those of IL-12. The detailed kinetics induced by each T. gondii antigen were different from each other.

Citations

Citations to this article as recorded by

Differential detection of chicken heterodimeric cytokines, interleukin 12 and 23 using their subunit-specific mouse monoclonal antibodies
Youngsub Lee, Woo H. Kim, Hyoyoun Nam, Hyun S. Lillehoj
Poultry Science.2024; 103(8): 103872. CrossRef
T. gondii excretory proteins promote the osteogenic differentiation of human bone mesenchymal stem cells via the BMP/Smad signaling pathway
Mingzhu Deng, Feifei Gao, Tianfeng Liu, Weiqiang Zhan, Juanhua Quan, Ziquan Zhao, Xuyang Wu, Zhuolan Zhong, Hong Zheng, Jiaqi Chu
Journal of Orthopaedic Surgery and Research.2024;[Epub] CrossRef
IL-12 and IL-23 Production in Toxoplasma gondii- or LPS Treated Jurkat T Cells via PI3K and MAPK Signaling Pathways
Hassan Ahmed Hassan Ahmed Ismail, Byung-Hun Kang, Jae-Su Kim, Jae-Hyung Lee, In-Wook Choi, Guang-Ho Cha, Jae-Min Yuk, Young-Ha Lee
The Korean Journal of Parasitology.2017; 55(6): 613. CrossRef
Cytokine response of human THP-1 macrophages to Trichomonas tenax
Emily J. Govro, Melissa K. Stuart
Experimental Parasitology.2016; 169: 77. CrossRef
Far beyond Phagocytosis: Phagocyte-Derived Extracellular Traps Act Efficiently against Protozoan ParasitesIn VitroandIn Vivo
Liliana M. R. Silva, Tamara Muñoz-Caro, Rafael A. Burgos, Maria A. Hidalgo, Anja Taubert, Carlos Hermosilla
Mediators of Inflammation.2016; 2016: 1. CrossRef
Eimeria ninakohlyakimovae induces NADPH oxidase-dependent monocyte extracellular trap formation and upregulates IL-12 and TNF-α, IL-6 and CCL2 gene transcription
D. Pérez, M.C. Muñoz, J.M. Molina, T. Muñoz-Caro, L.M.R. Silva, A. Taubert, C. Hermosilla, A. Ruiz
Veterinary Parasitology.2016; 227: 143. CrossRef
Secretion of Rhoptry and Dense Granule Effector Proteins by Nonreplicating Toxoplasma gondii Uracil Auxotrophs Controls the Development of Antitumor Immunity
Barbara A. Fox, Kiah L. Sanders, Leah M. Rommereim, Rebekah B. Guevara, David J. Bzik, Imtiaz A Khan
PLOS Genetics.2016; 12(7): e1006189. CrossRef
Levels of Transforming Growth Factor-Beta After Immunization of Mice With Toxoplasma gondii prepared Excretory/Secretory Proteins
Seyed Hossein Abdollahi, Fateme Ayoobi, Hossein Khorramdelazad, Behzad Nasiri Ahmadabadi, Mohammadtaghi Rezayati, Mohammad Kazemi Arababadi, Mohammad Zare-Bidaki
Jundishapur Journal of Microbiology.2015;[Epub] CrossRef
Intracellular Networks of the PI3K/AKT and MAPK Pathways for Regulating Toxoplasma gondii-Induced IL-23 and IL-12 Production in Human THP-1 Cells
Juan-Hua Quan, Jia-Qi Chu, Jaeyul Kwon, In-Wook Choi, Hassan Ahmed Hassan Ahmed Ismail, Wei Zhou, Guang-Ho Cha, Yu Zhou, Jae-Min Yuk, Eun-Kyeong Jo, Young-Ha Lee, Salvatore V Pizzo
PLOS ONE.2015; 10(11): e0141550. CrossRef
Besnoitia besnoiti tachyzoites induce monocyte extracellular trap formation
Tamara Muñoz-Caro, Liliana M. R. Silva, Christin Ritter, Anja Taubert, Carlos Hermosilla
Parasitology Research.2014; 113(11): 4189. CrossRef

12,564 View
112 Download
Crossref

Gene Expression Profiles in Genetically Different Mice Infected with Toxoplasma gondii: ALDH1A2, BEX2, EGR2, CCL3 and PLAU: Hassan Ahmed Hassan Ahmed Ismail, Juan-Hua Quan, Zhou Wei, In-Wook Choi, Guang-Ho Cha, Dae-Whan Shin, Young-Ha Lee, Chang-June Song; Korean J Parasitol 2012;50(1):7-13.
Published online March 6, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.1.7

Abstract
PDF

Toxoplasma gondii can modulate host cell gene expression; however, determining gene expression levels in intermediate hosts after T. gondii infection is not known much. We selected 5 genes (ALDH1A2, BEX2, CCL3, EGR2 and PLAU) and compared the mRNA expression levels in the spleen, liver, lung and small intestine of genetically different mice infected with T. gondii. ALDH1A2 mRNA expressions of both mouse strains were markedly increased at day 1-4 postinfection (PI) and then decreased, and its expressions in the spleen and lung were significantly higher in C57BL/6 mice than those of BALB/c mice. BEX2 and CCR3 mRNA expressions of both mouse strains were significantly increased from day 7 PI and peaked at day 15-30 PI (P<0.05), especially high in the spleen liver or small intestine of C57BL/6 mice. EGR2 and PLAU mRNA expressions of both mouse strains were significantly increased after infection, especially high in the spleen and liver. However, their expression patterns were varied depending on the tissue and mouse strain. Taken together, T. gondii-susceptible C57BL/6 mice expressed higher levels of these 5 genes than did T. gondii-resistant BALB/c mice, particularly in the spleen and liver. And ALDH1A2 and PLAU expressions were increased acutely, whereas BEX2, CCL3 and EGR2 expressions were increased lately. Thus, these demonstrate that host genetic factors exert a strong impact on the expression of these 5 genes and their expression patterns were varied depending on the gene or tissue.

Citations

Citations to this article as recorded by

Effect of Puerarin, Baicalin and Berberine Hydrochloride on the Regulation of IPEC-J2 Cells Infected with Enterotoxigenic Escherichia coli
Xiaoxi Liu, Fenghua Liu, Yunfei Ma, Huanrong Li, Xianghong Ju, Jianqin Xu
Evidence-Based Complementary and Alternative Medicine.2019; 2019: 1. CrossRef
Gene Silencing of Porcine MUC13 and ITGB5: Candidate Genes towards Escherichia coli F4ac Adhesion
Chuanli Zhou, Zhengzhu Liu, Yang Liu, Weixuan Fu, Xiangdong Ding, Jianfeng Liu, Ying Yu, Qin Zhang, Lucia R. Languino
PLoS ONE.2013; 8(7): e70303. CrossRef

10,065 View
86 Download
Crossref

Brief Communication

Genotyping of a Korean isolate of Toxoplasma gondii by multilocus PCR-RFLP and microsatellite analysis: Juan-Hua Quan, Tae Yun Kim, In-Uk Choi, Young-Ha Lee; Korean J Parasitol 2008;46(2):105-108.
Published online June 20, 2008; DOI: https://doi.org/10.3347/kjp.2008.46.2.105

Abstract
PDF

Although the Korean isolate KI-1 of Toxoplasma gondii has been considered to be a virulent type I lineage because of its virulent clinical manifestations, its genotype is unclear. In the present study, genotyping of the KI-1 was performed by multilocus PCR-RFLP and microsatellite sequencing. For 9 genetic markers (c22-8, c29-2, L358, PK1, SAG2, SAG3, GRA6, BTUB, and Apico), the KI-1 and RH strains exhibited typical PCR-RFLP patterns identical to the type I strains. DNA sequencing of tandem repeats in 5 microsatellite markers (B17, B18, TUB2, W35, and TgM-A) of the KI-1 also revealed patterns characteristic of the type I. These results provide strong genetic evidence that KI-1 is a type I lineage of T. gondii.

Citations

Citations to this article as recorded by

Far-East Asian Toxoplasma isolates share ancestry with North and South/Central American recombinant lineages
Fumiaki Ihara, Hisako Kyan, Yasuhiro Takashima, Fumiko Ono, Kei Hayashi, Tomohide Matsuo, Makoto Igarashi, Yoshifumi Nishikawa, Kenji Hikosaka, Hirokazu Sakamoto, Shota Nakamura, Daisuke Motooka, Kiyoshi Yamauchi, Madoka Ichikawa-Seki, Shinya Fukumoto, Mo
Nature Communications.2024;[Epub] CrossRef
Clinical characteristics of toxoplasmosis patients in Korea: A retrospective study using health insurance review and assessment service data and electronic medical records
Do-Won Ham, Bong-Kwang Jung, Ji-Hun Shin, Yong Joon Kim, Kyoung Yul Seo, Seung Mi Lee, Jae Hyoung Im, Jeong-Ran Kwon, Ho-Sung Lee, Kyung-Won Hwang, Eun-Hee Shin
Parasites, Hosts and Diseases.2024; 62(4): 424. CrossRef
Application of Toxoplasma gondii GRA15 peptides in diagnosis and serotyping
Runli Li, Yeting Ma, Jin Li, Penglai Zhou, Fuguo Zheng, Qing Liu, Wenwei Gao
Microbial Pathogenesis.2020; 143: 104168. CrossRef
An outbreak of toxoplasmosis in squirrel monkeys (Saimiri sciureus) in South Korea
Hanseul Oh, Kyung‐Yeon Eo, Sanjeev Gumber, Jung Joo Hong, C‐Yoon Kim, Hyun‐Ho Lee, Young‐Mok Jung, Jin Kim, Gyu‐Whan Whang, Ji‐Min Lee, Yong‐Gu Yeo, Bokyeong Ryu, Ji‐Sook Ryu, Seul‐Kee Lee, Ukjin Kim, Sin‐Geun Kang, Jae‐Hak Park
Journal of Medical Primatology.2018; 47(4): 238. CrossRef
Geographical distribution of Toxoplasma gondii genotypes in Asia: A link with neighboring continents
P. Chaichan, A. Mercier, L. Galal, A. Mahittikorn, F. Ariey, S. Morand, F. Boumédiène, R. Udonsom, A. Hamidovic, J.B. Murat, Y. Sukthana, M.L. Dardé
Infection, Genetics and Evolution.2017; 53: 227. CrossRef
Diagnosis of toxoplasmosis and typing of Toxoplasma gondii
Quan Liu, Ze-Dong Wang, Si-Yang Huang, Xing-Quan Zhu
Parasites & Vectors.2015;[Epub] CrossRef
Genetic characterization of Toxoplasma gondii isolates from chickens in India by GRA6 gene sequence analysis
Shantaveer Biradar, Buddhi Saravanan, Anup Tewari, Chirukandoth Sreekumar, Muthu Sankar, Naduvanahalli Sudhakar
Acta Parasitologica.2014;[Epub] CrossRef
Detection of Toxoplasma gondii DNA in Sheep and Goat Milk in Northwest of Iran by PCR-RFLP
Mousa Tavassoli, Bijan Esmaeilnejad, Farnaz Malekifard, Ali Soleimanzadeh, Mahdi Dilmaghani
Jundishapur Journal of Microbiology.2013;[Epub] CrossRef
Isolation and Genotyping of Toxoplasma gondii from Free-Range Ducks in Malaysia
Vignesh R. Puvanesuaran, Rahmah Noordin, Venugopal Balakrishnan
Avian Diseases.2013; 57(1): 128. CrossRef
Isolation and characterization of Toxoplasma gondii strains from stray cats revealed a single genotype in Beijing, China
Weifeng Qian, Hui Wang, Chunlei Su, Dan Shan, Xia Cui, Na Yang, Chaochao Lv, Qun Liu
Veterinary Parasitology.2012; 187(3-4): 408. CrossRef
Genotype of Toxoplasma gondii from Blood of Stray Cats in Gyeonggi-do, Korea
Hye-Youn Kim, Yun-Ah Kim, Ho Sa Lee, Ho Gun Rhie, Shin-Hyeong Cho, Jae-Ran Yu, Sang-Eun Lee
The Korean Journal of Parasitology.2009; 47(4): 413. CrossRef