To evaluate the seroprevalence against circumsporozoite protein (CSP) of Plasmodium vivax in sera of Korean patients, the central repeating domain (CRD) of CSP was cloned and analyzed. From the genomic DNA of patient's blood, 2 kinds of CSPs were identified to belong to a VK210 type, which is the dominant repeating of GDRA(D/A)GQPA, and named as PvCSPA and PvCSPB. Recombinantly expressed his-tagged PvCSPA or PvCSPB in Escherichia coli reacted well against sera of patients in western blot, with the detecting rate of 47.9% (58/121), which included 15 cases positive for PvCSPA, 6 cases positive for PvCSPB, and 37 cases for both. The mixture of PvCSPA and PvCSPB was loaded to a rapid diagnostic test kit (RDT) and applied with the same set of patient sera, which resulted in detection rates of 57.0% (69/121). When the protein sequences of PvCSPA were compared with those of P. vivax in endemic regions of India and Uganda, they were compatibly homologous to PvCSPA with minor mutations. These results suggested that the recombinant PvCSPA and PvCSPB loaded RDT may be a milestone in latent diagnosis which has been a hot issue of domestic malaria and important for radical therapy in overlapped infections with P. falciparum in tropical and subtropical areas. During the biological process of malarial infection, exposure of CSP to antigen-antibody reaction up to 57.0% is the first report in Korea.
Citations
Citations to this article as recorded by
Using Serological Markers for the Surveillance of Plasmodium vivax Malaria: A Scoping Review Lejla Kartal, Ivo Mueller, Rhea J. Longley Pathogens.2023; 12(6): 791. CrossRef
A Dual, Systematic Approach to Malaria Diagnostic Biomarker Discovery Seda Yerlikaya, Ewurama D A Owusu, Augustina Frimpong, Robert Kirk DeLisle, Xavier C Ding Clinical Infectious Diseases.2022; 74(1): 40. CrossRef
Structure-genetic diversity and recombinant protein of circumsporozoite protein (CSP) of vivax malaria antigen: A potential malaria vaccine candidate Vahid Raissi, Soudabeh Etemadi, Muhammad Ibrahim Getso, Ahmad Mehravaran, Omid Raiesi Gene Reports.2021; 23: 101132. CrossRef
Serological responses to a soluble recombinant circumsporozoite protein-VK210 of Plasmodium vivax (rPvCSP-VK210) among Iranian malaria patients Mehdi Nateghpour, Soudabeh Etemadi, Afsaneh Motevalli Haghi, Hamid Eslami, Mehdi Mohebali, Leila Farivar European Journal of Medical Research.2021;[Epub] CrossRef
Recent Advances in the Development of Biosensors for Malaria Diagnosis Francis D. Krampa, Yaw Aniweh, Prosper Kanyong, Gordon A. Awandare Sensors.2020; 20(3): 799. CrossRef
Genetic Diversity of Plasmodium vivax Causing Epidemic Malaria in the Republic of Korea Young Yil Bahk, Jeonga Kim, Seong Kyu Ahn, Byoung-Kuk Na, Jong-Yil Chai, Tong-Soo Kim The Korean Journal of Parasitology.2018; 56(6): 545. CrossRef
Plasmodium vivax msp-3α polymorphisms: analysis in the Indian subcontinent Anju Verma, Hema Joshi, Vineeta Singh, Anup Anvikar, Neena Valecha Malaria Journal.2016;[Epub] CrossRef
Toxoplasma gondii is an apicomplexan parasite with a broad host range of most warm-blooded mammals including humans, of which one-thirds of the human population has been infected worldwide which can cause congenital defects, abortion, and neonatal complications. Here, we developed a rapid diagnostic test (RDT) for T. gondii infection. Antigenic N-terminal half of the major surface antigen (SAG1) was linked with intrinsically unstructured domain (IUD) of dense granule protein 2 (GRA2). The recombinant GST-GRA2-SAG1A protein was successfully expressed and purified as 51 kDa of molecular weight. Furthermore, antigenicity and solubility of the rGST-GRA2-SAG1A protein were significantly increased. The overall specificity and sensitivity of GST-GRA2-SAG1A loaded RDT (TgRDT) were estimated as 100% and 97.1% by comparing with ELISA result which uses T. gondii whole cell lysates as the antigen. The TgRDT tested with Uganda people sera for field trial and showed 31.9% of seroprevalence against T. gondii antibody. The TgRDT is proved to be a kit for rapid and easy to use with high accuracy, which would be a suitable serodiagnostic tool for toxoplasmosis.
Citations
Citations to this article as recorded by
Development of an Immunochromatographic Test with Recombinant MIC2-MIC3 Fusion Protein for Serological Detection of Toxoplasma gondii Jianzhong Wang, Yi Zhao, Jicheng Qiu, Jing Liu, Rui Zhou, Xialin Ma, Xiaojie Wu, Xiaoguang Li, Wei Mao, Yiduo Liu, Heng Zhang Veterinary Sciences.2025; 12(6): 509. CrossRef
Application of gold immunochromatographic assay strip combined with digital evaluation for early detection of Toxoplasma gondii infection in multiple species Jiyuan Fan, Hao Sun, Jiawen Fang, Yafan Gao, Haojie Ding, Bin Zheng, Qingming Kong, Xunhui Zhuo, Shaohong Lu Parasites & Vectors.2024;[Epub] CrossRef
Single Cell Expression Systems for the Production of Recombinant Proteins for Immunodiagnosis and Immunoprophylaxis of Toxoplasmosis Karolina Sołowińska, Lucyna Holec-Gąsior Microorganisms.2024; 12(8): 1731. CrossRef
Seroprevalence of Toxoplasma gondii infection among patients of a tertiary hospital in Guangzhou, Guangdong province, PR China Yu-bin Guan, Xiao-xiao Sun, Shao-lian Chen, Xiao-ting Zhu, Zhi-hua Zeng, Han-wei Lu, Hong-mei Feng, Yu Guo, Wen-gong Jiang, Kui Xiong, Xiao-rong Yang, Ho-Woo Nam, Zhao-shou Yang, Pan Li PLOS ONE.2023; 18(7): e0286430. CrossRef
Past and present seroprevalence and disease burden estimates of Toxoplasma gondii infections in Germany: An appreciation of the role of serodiagnostics Frank Seeber International Journal of Medical Microbiology.2023; 313(6): 151592. CrossRef
Seroprevalence of Toxoplasma gondii assayed using Rapid Diagnostic Tests among Residents in Three Counties Adjacent to The Demilitarized Zone, Korea Jeehi Jung, Jinyoung Lee, Yoon Kyung Chang, Seong Kyu Ahn, Seo Hye Park, Sung-Jong Hong, Jihoo Lee, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam, Tong-Soo Kim, Dongjae Kim The Korean Journal of Parasitology.2021; 59(1): 9. CrossRef
Clusters of Toxoplasmosis in Ganghwa-gun, Cheorwon-gun, and Goseong-gun, Korea Jihye Yu, Woojin Kim, Yoon Kyung Chang, Tong-Soo Kim, Sung-Jong Hong, Hye-Jin Ahn, Ho-Woo Nam, Dongjae Kim The Korean Journal of Parasitology.2021; 59(3): 251. CrossRef
Serological and molecular rapid diagnostic tests for Toxoplasma infection in humans and animals Amjad Hayat Khan, Rahmah Noordin European Journal of Clinical Microbiology & Infectious Diseases.2020; 39(1): 19. CrossRef
Clusters of Toxoplasmosis in Gyodong-Myeon and Samsan-Myeon, Ganghwa-Gun, Korea Woojin Kim, Yoon Kyung Chang, Tong-Soo Kim, Sung-Jong Hong, Hye-Jin Ahn, Ho-Woo Nam, Dongjae Kim The Korean Journal of Parasitology.2020; 58(5): 493. CrossRef
Identification of novel antigens for serum IgG diagnosis of human toxoplasmosis Juntao Luo, Jingyi Wan, Ziru Tang, Shuang Shen Experimental Parasitology.2019; 204: 107722. CrossRef
Seroprevalence of Toxoplasmosis with ELISA and Rapid Diagnostic Test among Residents in Gyodong-do, Inchon city, Korea: A Four-Year Follow-up Yeong Hoon Kim, Ji hoo Lee, Seong kyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam The Korean Journal of Parasitology.2017; 55(3): 247. CrossRef
Development of direct assays for Toxoplasma gondii and its use in genomic DNA sample Lívia M. Alves, Vinícius R. Rodovalho, Ana C.H. Castro, Márcia A.R. Freitas, Caroline M. Mota, Tiago W.P. Mineo, José R. Mineo, João M. Madurro, Ana G. Brito-Madurro Journal of Pharmaceutical and Biomedical Analysis.2017; 145: 838. CrossRef
Seroprevalence of Toxoplasmosis Detected by RDT in Residents near the DMZ (demilitarized zone) of Cheorwon-gun, Gangwon-do, Korea Yeong Hoon Kim, Jihoo Lee, Young-Eun Kim, Seongkyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam The Korean Journal of Parasitology.2017; 55(4): 385. CrossRef
Western Blot Detection of Human Anti-Chikungunya Virus Antibody with Recombinant Envelope 2 Protein Zhaoshou Yang, Jihoo Lee, Hye-Jin Ahn, Chom-Kyu Chong, Ronaldo F. Dias, Ho-Woo Nam The Korean Journal of Parasitology.2016; 54(2): 239. CrossRef
Digested disorder, Quarterly intrinsic disorder digest (October-November-December, 2013) Shelly DeForte, Krishna D Reddy, Vladimir N Uversky Intrinsically Disordered Proteins.2015; 3(1): e984569. CrossRef
Probability of Antibody Formation against Circumsporozoite Protein of Plasmodium vivax among Korean Malaria Patients Ho-Woo Nam, Kyoung Ju Song, Hye Jin Ahn, Zhaoshou Yang, Chom-Kyu Chong, Pyo Yun Cho, Seong Kyu Ahn, Tong-Soo Kim The Korean Journal of Parasitology.2014; 52(2): 143. CrossRef
High Expression of Water-Soluble Recombinant Antigenic Domains ofToxoplasma gondii Secretory Organelles Zhaoshou Yang, Hye-Jin Ahn, Ho-Woo Nam The Korean Journal of Parasitology.2014; 52(4): 367. CrossRef
Toxoplasma gondii penetrates all kinds of nucleated eukaryotic cells but modulates host cells differently for its intracellular survival. In a previous study, we found out that serine protease inhibitors B3 and B4 (SERPIN B3/B4 because of their very high homology) were significantly induced in THP-1-derived macrophages infected with T. gondii through activation of STAT6. In this study, to evaluate the effects of the induced SERPIN B3/B4 on the apoptosis of T. gondii-infected THP-1 cells, we designed and tested various small interfering (si-) RNAs of SERPIN B3 or B4 in staurosporine-induced apoptosis of THP-1 cells. Anti-apoptotic characteristics of THP-1 cells after infection with T. gondii disappeared when SERPIN B3/B4 were knock-downed with gene specific si-RNAs transfected into THP-1 cells as detected by the cleaved caspase 3, poly-ADP ribose polymerase and DNA fragmentation. This anti-apoptotic effect was confirmed in SERPIN B3/B4 overexpressed HeLa cells. We also investigated whether inhibition of STAT6 affects the function of SERPIN B3/B4, and vice versa. Inhibition of SERPIN B3/B4 did not influence STAT6 expression but SERPIN B3/B4 expression was inhibited by STAT6 si-RNA transfection, which confirmed that SERPIN B3/B4 was induced under the control of STAT6 activation. These results suggest that T. gondii induces SERPIN B3/B4 expression via STAT6 activation to inhibit the apoptosis of infected THP-1 cells for longer survival of the intracellular parasites themselves.
Citations
Citations to this article as recorded by
Identification and functional analysis of a serine protease inhibitor using machine learning strategy Heqian Zhang, Yaxin Wu, Yanran Zhu, Liangjun Ge, Jiaquan Huang, Zhiwei Qin International Journal of Biological Macromolecules.2024; 265: 130852. CrossRef
Overview of Apoptosis, Autophagy, and Inflammatory Processes in Toxoplasma gondii Infected Cells Ehsan Ahmadpour, Farhad Babaie, Tohid Kazemi, Sirous Mehrani Moghaddam, Ata Moghimi, Ramin Hosseinzadeh, Veeranoot Nissapatorn, Abdol Sattar Pagheh Pathogens.2023; 12(2): 253. CrossRef
Modulation of STAT-1, STAT-3, and STAT-6 activities in THP-1 derived macrophages infected with two Trypanosoma cruzi strains Melissa Martins Oliveira, Camila Ramalho Bonturi, Bruno Ramos Salu, Maria Luiza Vilela Oliva, Renato Arruda Mortara, Cristina Mary Orikaza Frontiers in Immunology.2022;[Epub] CrossRef
BEWO trophoblast cells and Toxoplasma gondii infection modulate cell death mechanisms in THP-1 monocyte cells by interference in the expression of death receptor and intracellular proteins Andressa da Silva Castro, Mariana Bodini Angeloni, Bellisa de Freitas Barbosa, Renata Lima de Miranda, Samuel Cota Teixeira, Pâmela Mendonça Guirelli, Fernanda Chaves de Oliveira, Rafaela José da Silva, Priscila Silva Franco, Mayara Ribeiro, Iliana Claudi Tissue and Cell.2021; 73: 101658. CrossRef
Transcriptional profiling of human macrophages during infection withBordetella pertussis Denisa Petráčková, Mariam R. Farman, Fabian Amman, Irena Linhartová, Ana Dienstbier, Dilip Kumar, Jakub Držmíšek, Ivo Hofacker, Maria Eugenia Rodriguez, Branislav Večerek RNA Biology.2020; 17(5): 731. CrossRef
Secretome Analysis of Host Cells Infected with Toxoplasma gondii after Treatment of Human Epidermal Growth Factor Receptor 2/4 Inhibitors Hye-Jung Kim, Hye-Jin Ahn, Hyeweon Kang, Jaehui Park, Seul gi Oh, Saehae Choi, Won-Kyu Lee, Ho-Woo Nam The Korean Journal of Parasitology.2020; 58(3): 249. CrossRef
Toxoplasma gondiiModulates the Host Cell Responses: An Overview of Apoptosis Pathways Nour Mammari, Mohamad Adnan Halabi, Souha Yaacoub, Hilda Chlala, Marie-Laure Dardé, Bertrand Courtioux BioMed Research International.2019; 2019: 1. CrossRef
Mechanisms of Human Innate Immune Evasion by Toxoplasma gondii Tatiane S. Lima, Melissa B. Lodoen Frontiers in Cellular and Infection Microbiology.2019;[Epub] CrossRef
Strategies Developed by Toxoplasma gondii to Survive in the Host Wanbo Zhu, Jingyang Li, Faustina Pappoe, Jilong Shen, Li Yu Frontiers in Microbiology.2019;[Epub] CrossRef
Identification of an interaction between calcium-dependent protein kinase 4 (EtCDPK4) and serine protease inhibitor (EtSerpin) in Eimeria tenella Ling Lv, Bing Huang, Qiping Zhao, Zongping Zhao, Hui Dong, Shunhai Zhu, Ting Chen, Ming Yan, Hongyu Han Parasites & Vectors.2018;[Epub] CrossRef
Quantitative proteomic analysis of host epithelial cells infected by Salmonella enterica serovar Typhimurium Linlu Qi, Mo Hu, Jiaqi Fu, Yanhua Liu, Mei Wu, Kaiwen Yu, Xiaoyun Liu PROTEOMICS.2017;[Epub] CrossRef
Phosphoproteome of Toxoplasma gondii Infected Host Cells Reveals Specific Cellular Processes Predominating in Different Phases of Infection Hai-Xia Wei, Ai-Yuan Chen, Cheng He, Hong-Juan Peng, Xiao-Shuang Feng The American Journal of Tropical Medicine and Hygiene.2017; 97(1): 236. CrossRef
Host-Toxoplasma gondii Coadaptation Leads to Fine Tuning of the Immune Response Thaís Rigueti Brasil, Celio Geraldo Freire-de-Lima, Alexandre Morrot, Andrea Cristina Vetö Arnholdt Frontiers in Immunology.2017;[Epub] CrossRef
Interferon-γ Protects from Staphylococcal Alpha Toxin-Induced Keratinocyte Death through Apolipoprotein L1 Anne M. Brauweiler, Elena Goleva, Donald Y.M. Leung Journal of Investigative Dermatology.2016; 136(3): 658. CrossRef
SERPINB3/B4 Contributes to Early Inflammation and Barrier Dysfunction in an Experimental Murine Model of Atopic Dermatitis Umasundari Sivaprasad, Kayla G. Kinker, Mark B. Ericksen, Mark Lindsey, Aaron M. Gibson, Stacey A. Bass, Nicolas S. Hershey, Jingyuan Deng, Mario Medvedovic, Gurjit K. Khurana Hershey Journal of Investigative Dermatology.2015; 135(1): 160. CrossRef
Trophoblast-macrophage crosstalk on human extravillous under Toxoplasma gondii infection P.M. Guirelli, M.B. Angeloni, B.F. Barbosa, A.O. Gomes, A.S. Castro, P.S. Franco, R.J. Silva, J.G. Oliveira, O.A. Martins-Filho, J.R. Mineo, F. Ietta, E.A. Ferro Placenta.2015; 36(10): 1106. CrossRef
Toxoplasma gondii inhibits apoptosis via a novel STAT3-miR-17–92-Bim pathway in macrophages Yihong Cai, He Chen, Xuwei Mo, Yuanyuan Tang, Xiucai Xu, Aimei Zhang, Zhaorong Lun, Fangli Lu, Yong Wang, Jilong Shen Cellular Signalling.2014; 26(6): 1204. CrossRef
The role of STAT-6 as a key transcription regulator in HeLa cell death induced by IFN-γ/TNF-α co-immobilized on nanoparticles Zhibin Li, Yan-Qing Guan, Jun-Ming Liu Biomaterials.2014; 35(18): 5016. CrossRef
Adaptive Evolution and Divergence of SERPINB3: A Young Duplicate in Great Apes Sílvia Gomes, Patrícia I. Marques, Rune Matthiesen, Susana Seixas, Charaf Benarafa PLoS ONE.2014; 9(8): e104935. CrossRef
Risk factors associated with seroprevalence of Neospora caninum in dogs from urban and rural areas of milk and coffee production in Minas Gerais state, Brazil C. I. NOGUEIRA, L. P. MESQUITA, C. C. ABREU, K. Y. R. NAKAGAKI, J. N. SEIXAS, P. S. BEZERRA, C. M. B. M. ROCHA, A. M. GUIMARAES, A. P. PECONICK, M. S. VARASCHIN Epidemiology and Infection.2013; 141(11): 2286. CrossRef
In-depth proteomic analysis of the human cerumen—A potential novel diagnostically relevant biofluid Martin Andreas Feig, Elke Hammer, Uwe Völker, Nico Jehmlich Journal of Proteomics.2013; 83: 119. CrossRef
Serpins, Immunity and Autoimmunity: Old Molecules, New Functions Mariele Gatto, Luca Iaccarino, Anna Ghirardello, Nicola Bassi, Patrizia Pontisso, Leonardo Punzi, Yehuda Shoenfeld, Andrea Doria Clinical Reviews in Allergy & Immunology.2013; 45(2): 267. CrossRef
STAT3-dependent transactivation of miRNA genes following Toxoplasma gondii infection in macrophage Yihong Cai, He Chen, Lei Jin, Yibo You, Jilong Shen Parasites & Vectors.2013;[Epub] CrossRef
Differential apoptosis in BeWo cells after infection with highly (RH) or moderately (ME49) virulent strains of Toxoplasma gondii is related to the cytokine profile secreted, the death receptor Fas expression and phosphorylated ERK1/2 expression M.B. Angeloni, P.M. Guirelli, P.S. Franco, B.F. Barbosa, A.O. Gomes, A.S. Castro, N.M. Silva, O.A. Martins-Filho, T.W.P. Mineo, D.A.O. Silva, J.R. Mineo, E.A.V. Ferro Placenta.2013; 34(11): 973. CrossRef
Two novel squamous cell carcinoma antigen-derived HLA-A*0201-binding peptides induce in vitro and in vivo CD8+ cytotoxic T lymphocyte responses ZHI-LIANG DUAN, ZHI-BIN WANG, JIANG-LONG GUO, WEN-QUAN LIU, JUN HU, JING LI, SI-NA WANG, QIANG LI, JIN-SHENG WEN International Journal of Oncology.2013; 42(4): 1482. CrossRef
First
Prev
