In this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against Neospora caninum in cattle. Sera from 150 cattle with a recent history of abortion were collected and tested by commercial ELISA kit and a standardized in-house dye immunoassay system. The positivity rate for the sera used in this study was 34.6% for the disperse dye immunoassay (DDIA) compared to 32% obtained by ELISA kit. This study showed no significant difference between DDIA and ELISA. The results indicated that the DDIA provide an economic, simple, rapid and robust test for detection of N. caninum infection in cattle.
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A novel immunochromatographic assay using ultramarine blue particles as visible label for quantitative detection of hepatitis B virus surface antigen Jing Liu, Qiongqiong Yu, Guangying Zhao, Wenchao Dou Analytica Chimica Acta.2020; 1098: 140. CrossRef
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Markers related to the diagnosis and to the risk of abortion in bovine neosporosis Sonia Almería, Fernando López-Gatius Research in Veterinary Science.2015; 100: 169. CrossRef
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