Parasites, Hosts and Diseases

"Min Hu"

Brief Communication

Development of a Lateral Flow Strip-Based Recombinase Polymerase Amplification Assay for the Detection of Haemonchus contortus in Goat Feces: Yao-Dong Wu, Qi-Qi Wang, Meng Wang, Hany M. Elsheikha, Xin Yang, Min Hu, Xing-Quan Zhu, Min-Jun Xu; Korean J Parasitol 2021;59(2):167-171.
Published online April 22, 2021; DOI: https://doi.org/10.3347/kjp.2021.59.2.167

Haemonchosis remains a significant problem in small ruminants. In this study, the assay of recombinase polymerase amplification (RPA) combined with the lateral flow strip (LFS-RPA) was established for the rapid detection of Haemonchus contortus in goat feces. The assay used primers and a probe targeting a specific sequence in the ITS-2 gene. We compared the performance of the LFS-RPA assay to a PCR assay. The LFS-RPA had a detection limit of 10 fg DNA, which was 10 times less compared to the lowest detection limit obtained by PCR. Out of 24 goat fecal samples, LFS-RPA assay detected H. contortus DNA with 95.8% sensitivity, compared to PCR, 79.1% sensitivity. LFS-RPA assay did not detect DNA from other related helminth species and demonstrated an adequate tolerance to inhibitors present in the goat feces. Taken together, our results suggest that LFS-RPA assay had a high diagnostic accuracy for the rapid detection of H. contortus and merits further evaluation.

Citations

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An RPA-CRISPR/Cas12a-assisted method for nucleic acid detection of Haemonchus contortus in sheep
Yutong Cao, Qiankun Yang, Yanbing Guo, Xiaocen Wang, Xin Li, Nan Zhang, Wenxue Lu, Jianhua Li, Xichen Zhang, Lili Cao, Pengtao Gong
Veterinary Parasitology.2025; 334: 110421. CrossRef
Rapid visual detection of Moniezia spp. in sheep feces via Recombinase Polymerase Amplification-Lateral Flow Dipstick (RPA-LFD) assay
Shaohua Zhang, Yeping Zhao, Weijia Liang, Shuai Wang, Xiu Cui, Haohan Zhu, Yueyue Zhang, Xiaolei Liu, Huimin Li, Wenjie Mu, Aijiang Guo
Veterinary Parasitology.2025; 339: 110582. CrossRef
Preliminary results of the recombinase polymerase amplification technique for the detection of Haemonchus contortus from Hungarian field samples
Rojesh Khangembam, Nóra Vass, Alison Morrison, Lynsey A. Melville, Alistair Antonopoulos, Levente Czeglédi
Veterinary Parasitology.2023; 320: 109974. CrossRef

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Original Article

Sensitivity of Plasmodium falciparum to Antimalarial Drugs in Hainan Island, China: Shan-Qing Wang, Guang-Ze Wang, Yu-Chun Li, Feng Meng, Shi-Gan Lin, Zhen-Hu Zhu, Ding-Wei Sun, Chang-Hua He, Xi-Min Hu, Jian-Wei Du; Korean J Parasitol 2015;53(1):35-41.
Published online February 27, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.1.35

Abstract
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Pyronaridine and artesunate have been shown to be effective in falciparum malaria treatment. However, pyronaridine is rarely used in Hainan Island clinically, and artesunate is not widely used as a therapeutic agent. Instead, conventional antimalarial drugs, chloroquine and piperaquine, are used, explaining the emergence of chloroquine-resistant Plasmodium falciparum. In this article, we investigated the sensitivity of P. falciparum to antimalarial drugs used in Hainan Island for rational drug therapy. We performed in vivo (28 days) and in vitro tests to determine the sensitivity of P. falciparum to antimalarial drugs. Total 46 patients with falciparum malaria were treated with dihydroartemisinin/piperaquine phosphate (DUO-COTECXIN) and followed up for 28 day. The cure rate was 97.8%. The mean fever clearance time (22.5±10.6 hr) and the mean parasite clearance time (27.3±12.2 hr) showed no statistical significance with different genders, ages, temperatures, or parasite density (P>0.05). The resistance rates of chloroquine, piperaquine, pyronarididine, and artesunate detected in vitro were 71.9%, 40.6%, 12.5%, and 0%, respectively (P<0.0001). The resistance intensities decreased as follows: chloroquine>piperaquine>pyronarididine>artesunate. The inhibitory dose 50 (IC₅₀) was 3.77×10^-6 mol/L, 2.09×10^-6 mol/L, 0.09×10^-6 mol/L, and 0.05×10^-6 mol/L, and the mean concentrations for complete inhibition (CIMC) of schizont formation were 5.60×10^-6 mol/L, 9.26×10^-6 mol/L, 0.55×10^-6 mol/L, and 0.07×10^-6 mol/L, respectively. Dihydroartemisinin showed a strong therapeutic effect against falciparum malaria with a low toxicity.

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Evaluation Algorithm of Volleyball Players’ Competitive Ability Based on the Random Matrix Model
Tailin Wang, Hua Zheng, Fangshu Li, Nian Jia, Zengliang Cai, Ning Cao
Mathematical Problems in Engineering.2022; 2022: 1. CrossRef

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104 Download
2 Web of Science
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Case Report

A Case of Plasmodium ovale wallikeri Infection in a Chinese Worker Returning from West Africa: Yuchun Li, Guangze Wang, Dingwei Sun, Feng Meng, Shigan Lin, Ximin Hu, Shanqing Wang; Korean J Parasitol 2013;51(5):557-562.
Published online October 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.5.557

Abstract
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In contrast to the gradual reduction in the number of locally transmitted malaria cases in China, the number of imported malaria cases has been increasing since 2008. Here, we report a case of a 39-year-old Chinese man who acquired Plasmodium ovale wallikeri infection while staying in Ghana, West Africa for 6 months in 2012. Microscopic examinations of Giemsa-stained thin and thick blood smears indicated Plasmodium vivax infection. However, the results of rapid diagnostic tests, which were conducted 3 times, were not in agreement with P. vivax. To further check the diagnosis, standard PCR analysis of the small-subunit rRNA gene was conducted, based on which a phylogeny tree was constructed. The results of gene sequencing indicated that this malaria is a variant of P. ovale (P. ovale wallikeri). The infection in this patient was not a new infection, but a relapse of the infection from the one that he had contracted in West Africa.

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Geographical origin of Plasmodium vivax in the Hainan Island, China: insights from mitochondrial genome
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Malaria Journal.2023;[Epub] CrossRef
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High frequency of the Duffy-negative genotype and absence of Plasmodium vivax infections in Ghana
Charles A. Brown, Prince J. Pappoe-Ashong, Nancy Duah, Anita Ghansah, Harry Asmah, Edwin Afari, Kwadwo A. Koram
Malaria Journal.2021;[Epub] CrossRef
A comparison of two PCR protocols for the differentiation of Plasmodium ovale species and implications for clinical management in travellers returning to Germany: a 10-year cross-sectional study
Hagen Frickmann, Christine Wegner, Stefanie Ruben, Ulrike Loderstädt, Egbert Tannich
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JustinJ.S. Wilcox, Abigail Kerschner, Hope Hollocher
Infection, Genetics and Evolution.2019; 75: 103994. CrossRef
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Ahmad Taghdiri, Pooya Ghasemi Nejad Almani, Iraj Sharifi, Mohammad Ali Mohammadi, Samira Salari
Microbial Pathogenesis.2019; 137: 103782. CrossRef
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Infection, Genetics and Evolution.2018;[Epub] CrossRef
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Jonathan Wee Kent Liew, Rohela Mahmud, Lian Huat Tan, Yee Ling Lau
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Clinical implications of a gradual dormancy concept in malaria
Joachim Richter, Gabriele Franken, Martha C. Holtfreter, Susanne Walter, Alfons Labisch, Heinz Mehlhorn
Parasitology Research.2016; 115(6): 2139. CrossRef
Genetic diversity of Plasmodium vivax population before elimination of malaria in Hainan Province, China
Yu-Chun Li, Guang-Ze Wang, Feng Meng, Wen Zeng, Chang-hua He, Xi-Min Hu, Shan-Qing Wang
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Molecular characterization of misidentified Plasmodium ovale imported cases in Singapore
Jean-Marc Chavatte, Sarah Bee Hui Tan, Georges Snounou, Raymond Tzer Pin Valentine Lin
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Characterization of Plasmodium ovale curtisi and P. ovale wallikeri in Western Kenya Utilizing a Novel Species-specific Real-time PCR Assay
Robin H. Miller, Clifford O. Obuya, Elizabeth W. Wanja, Bernhards Ogutu, John Waitumbi, Shirley Luckhart, V. Ann Stewart, Alain Debrabant
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