Parasites, Hosts and Diseases

"Min Kyoung Cho"

Brief Communication

Alteration of Cytokine Production during Visceral Larva Migrans by Toxascaris leonina in Mice: Shin Ae Kang, Mi-Kyung Park, Min Kyoung Cho, Hak Sun Yu; Korean J Parasitol 2013;51(5):583-588.
Published online October 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.5.583

Abstract
PDF

To determine alteration of immune responses during visceral larva migrans (VLM) caused by Toxascaris leonina at several time points, we experimentally infected mice with embryonated eggs of T. leonina and measured T-helper (Th) cell-related serial cytokine production after infection. At day 5 post infection (PI), most larvae were detected from the lungs, spleen, intestine, and muscle. Expression of thymic stromal lymphopoietin (TSLP) and CCL11 (eotaxin) showed a significant increase in most infected organs, except the intestine. However, expression of the CXCL1 (Gro-α) gene was most highly enhanced in the intestine at day 14 PI. Th1-related cytokine secretion of splenocytes showed increases at day 28 PI, and the level showed a decrease at day 42 PI. Th2-related cytokine secretion of splenocytes also showed an increase after infection; in particular, IL-5 level showed a significant increase at day 14 PI, and the level showed a decrease at day 28 PI. However, levels of Th17-related cytokines, IL-6 and IL-17A, showed gradual increases until day 42 PI. In conclusion, Th1, Th2, and Th17-related cytokine production might be important in immune responses against T. leonina VLM in experimental mice.

Citations

Citations to this article as recorded by

Histopathological lesions caused by experimental Toxocara canis and Toxascaris leonina infections in farm mink (Neovison vison)
Maciej Klockiewicz, Małgorzata Sobczak-Filipiak, Tadeusz Jakubowski, Ewa Długosz
Journal of Veterinary Research.2019; 63(2): 205. CrossRef
Experimental infection with T. canis and T. leonina in farm mink (Neovison vison)
Maciej Klockiewicz, Tadeusz Jakubowski, Małgorzata Sobczak-Filipiak, Justyna Bartosik, Ewa Długosz
Journal of Veterinary Research.2019; 63(2): 197. CrossRef

8,629 View
88 Download
Crossref

Original Article

Comparison of Functional Gene Annotation of Toxascaris leonina and Toxocara canis using CLC Genomics Workbench: Ki Uk Kim, Sang Kyun Park, Shin Ae Kang, Mi Kyung Park, Min Kyoung Cho, Ho-jin Jung, Kyung-Yun Kim, Hak Sun Yu; Korean J Parasitol 2013;51(5):525-530.
Published online October 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.5.525

The ascarids, Toxocara canis and Toxascaris leonina, are probably the most common gastrointestinal helminths encountered in dogs. In order to understand biological differences of 2 ascarids, we analyzed gene expression profiles of female adults of T. canis and T. leonina using CLC Genomics Workbench, and the results were compared with those of free-living nematode Caenorhabditis elegans. A total of 2,880 and 7,949 ESTs were collected from T. leonina and T. canis, respectively. The length of ESTs ranged from 106 to 4,637 bp with an average insert size of 820 bp. Overall, our results showed that most functional gene annotations of 2 ascarids were quite similar to each other in 3 major categories, i.e., cellular component, biological process, and molecular function. Although some different transcript expression categories were found, the distance was short and it was not enough to explain their different lifestyles. However, we found distinguished transcript differences between ascarid parasites and free-living nematodes. Understanding evolutionary genetic changes might be helpful for studies of the lifestyle and evolution of parasites.

Citations

Citations to this article as recorded by

CRISPR-Cas9 genome editing reveals that the Pgs gene of Fusarium circinatum is involved in pathogenicity, growth and sporulation
Alida van Dijk, Andi M. Wilson, Bianke Marx, Bianca Hough, Benedicta Swalarsk-Parry, Lieschen De Vos, Michael J. Wingfield, Brenda D. Wingfield, Emma T. Steenkamp
Fungal Genetics and Biology.2025; 177: 103970. CrossRef
Comparative genomics analysis reveals sequence characteristics potentially related to host preference in Cryptosporidium xiaoi
Jiayu Li, Yingying Fan, Na Li, Yaqiong Guo, Weijian Wang, Kangli Feng, Wei He, Falei Li, Jianbo Huang, Yanhua Xu, Lihua Xiao, Yaoyu Feng
International Journal for Parasitology.2024; 54(7): 379. CrossRef
Chromosome-level genome assembly of Cryptosporidium parvum by long-read sequencing of ten oocysts
Yuancai Chen, Jianying Huang, Huikai Qin, Kaihui Zhang, Yin Fu, Junqiang Li, Rongjun Wang, Kai Chen, Jie Xiong, Wei Miao, Guangying Wang, Longxian Zhang
Scientific Data.2024;[Epub] CrossRef
High subtelomeric GC content in the genome of a zoonotic Cryptosporidium species
Jiayu Li, Na Li, Dawn M. Roellig, Wentao Zhao, Yaqiong Guo, Yaoyu Feng, Lihua Xiao
Microbial Genomics .2023;[Epub] CrossRef
The Combination of Phages and Faecal Microbiota Transplantation Can Effectively Treat Mouse Colitis Caused by Salmonella enterica Serovar Typhimurium
Xinwu Wang, Yating Xing, Yalu Ji, Hengyu Xi, Xiaohe Liu, Li Yang, Liancheng Lei, Wenyu Han, Jingmin Gu
Frontiers in Microbiology.2022;[Epub] CrossRef
Complete Plastome of Physalis angulata var. villosa, Gene Organization, Comparative Genomics and Phylogenetic Relationships among Solanaceae
Xiaori Zhan, Zhenhao Zhang, Yong Zhang, Yadi Gao, Yanyun Jin, Chenjia Shen, Huizhong Wang, Shangguo Feng
Genes.2022; 13(12): 2291. CrossRef
Mitochondrial genes associated with pyrethroid resistance revealed by mitochondrial genome and transcriptome analyses in the malaria vector Anopheles sinensis (Diptera: Culicidae)
Yi‐Ran Ding, Zhen‐Tian Yan, Feng‐Ling Si, Xu‐Dong Li, Qi‐Meng Mao, Sana Asghar, Bin Chen
Pest Management Science.2020; 76(2): 769. CrossRef
Environmental microbiology: Perspectives for legal and occupational medicine
Saverio Giampaoli, Federica Alessandrini, Giovanni Vanni Frajese, Giovanni Guglielmi, Adriano Tagliabracci, Andrea Berti
Legal Medicine.2018; 35: 34. CrossRef

9,165 View
96 Download
Crossref

Brief Communications

Environmental Factors Related to Enterobiasis in a Southeast Region of Korea: Dong-Hee Kim, Min Kyoung Cho, Mi Kyung Park, Shin Ae Kang, Bo Young Kim, Sang Kyun Park, Hak Sun Yu; Korean J Parasitol 2013;51(1):139-142.
Published online February 18, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.1.139

Abstract
PDF

Pinworm infection can occur through contact with contaminated surfaces followed by ingestion or even through inhalation of infective eggs. We have limited information regarding environmental contamination by eggs of Enterobius vermicularis. In order to determine environmental risk factors associated with the rate of E. vermicularis infection, we investigated possible environmental risk factors using a questionnaire from 46 kindergartens in 3 different cities of the southeast area of Korea. In total, using the cellotape anal swab technique, 3,422 children were examined for E. vermicularis infection. We evaluated E. vermicularis egg of books, educational materials, toys, room door handles, dusts of window edges, desks, chairs, tables, and dusts of classrooms. The overall egg-positive rate for E. vermicularis was 6.0%, and the prevalence of enterobiasis in each kindergarten ranged between 0% and 16.9%. We found that 78.9% of egg positive kindergartens were managed by private foundations, which was significantly higher, compared with kindergartens managed by public foundations or the nation. Compared with public or national kindergartens, most private kindergartens were located in residential areas and the number of children in these areas was significantly higher. In conclusion, numbers of children in kindergartens was found to be an environmental risk factor associated with transmission of enterobiasis in Korea.

Citations

Citations to this article as recorded by

Pinworm (Enterobius Vermicularis) Infestation: An Updated Review
Alexander K.C. Leung, Joseph M. Lam, Benjamin Barankin, Alex H.C. Wong, Kin F. Leong, Kam L. Hon
Current Pediatric Reviews.2025; 21(4): 333. CrossRef
Enterobius vermicularis infection and its risk factors among rural schoolchildren in Zhejiang Province: a cross-sectional study
Jialie Jin, Wei Ruan, Wenjie Xu, Xuan Zhang, Kegen Yu, Hualiang Chen, Jiaqi Zhang, Jimin Sun, Jianmin Jiang, Xiaoxiao Wang
Parasitology Research.2025;[Epub] CrossRef
A 25-Year-Old Woman With Long-Lasting Abdominal Pain and Spleen Abscess
Anthony Amoroso, Priya Nori, David J Riedel, Paolo Agostinis, Dario Cappello, Niccolò Riccardi, Teresa Michelutti, Maria Orsaria, Verena Zerbato, Stefano Di Bella
Clinical Infectious Diseases.2023; 77(5): 795. CrossRef
Global prevalence of enterobiasis in young children over the past 20 years: a systematic review and meta-analysis
Elham Kia Lashaki, Azadeh Mizani, Seyed Abdollah Hosseini, Bentolhoda Habibi, Khadijeh Taherkhani, Amir Javadi, AliReza Taremiha, Samira Dodangeh
Osong Public Health and Research Perspectives.2023; 14(6): 441. CrossRef
Nutritional status, intestinal parasitic infections, and socio‐environmental conditions in Mbyá‐guaraní children: The current situation in communities incentral Misiones, Argentina
María Lorena Zonta, Andrea Servián, Guillermo Panisse, Evelia E. Oyhenart, Graciela T. Navone
American Journal of Human Biology.2022;[Epub] CrossRef
Enterobiasis among Yemeni children: a cross-sectional study
Abdulelah H. Al-Adhroey, Yahya A. Al-Ansi, Mohammed A. Al-Kholani, Abdulrahman H. Amer, Marwan M. Al-Khyat, Fadia H. Al Hubaishi, Radhwan H. Aziz, Ebrahim S. Al-Khateeb, Souad A. Al-Gabri, Tawfik M. Al-Gabri
Journal of Parasitic Diseases.2022; 46(3): 722. CrossRef
Is pinworm infection still a public health concern among children in resource-rich regions? Trends in pinworm infection prevalence and associated factors among children in Hualien County, Taiwan: a retrospective cross-sectional study
Yu-Chao Hsiao, Jen-Hung Wang, Chia-Hsiang Chu, Yu-Hsun Chang, Yung-Chieh Chang, Rong-Hwa Jan, Shao-Yin Chu, Shang-Hsien Yang, Jui-Shia Chen, Ming-Chun Chen
BMC Public Health.2022;[Epub] CrossRef
High case enterobiasis in school children and potential eggs distribution on the bed
Didik Sumanto, Sayono E, Wulandari Meikawati, Tri Dewi Kristini, Wahyu Handoyo, Aris Sugiharto
Journal of Microbiology & Experimentation.2022; 10(1): 33. CrossRef
Enterobius vermicularis Infection among Preschool Children: A 12-Year (2008-2019) Survey in Large Cities and Provinces of the Republic of Korea
Hyejoo Shin, Bong-Kwang Jung, Seungwan Ryoo, Sooji Hong, Taehee Chang, Jiyeon Park, Keon Hoon Lee, Jeonggyu Lee, Jae Young Park, Hoo-Gn Jeoung, Jae Hyun Cho, Jong-Yil Chai
The Korean Journal of Parasitology.2021; 59(4): 421. CrossRef
Genetic variation of Enterobius vermicularis among schoolchildren in Thailand
K. Tomanakan, O. Sanpool, P. Chamavit, V. Lulitanond, P.M. Intapan, W. Maleewong
Journal of Helminthology.2020;[Epub] CrossRef
Morphological characteristic of Skrjabinema ovis (Nematoda, Oxyuridae) obtained from domestic sheep
V. V. Melnychuk, O. I. Reshetylo
Regulatory Mechanisms in Biosystems.2020;[Epub] CrossRef
Detection of Enterobius vermicularis in greater Berlin, 2007–2017: seasonality and increased frequency of detection
Johannes Friesen, Clara Bergmann, Robert Neuber, Jörg Fuhrmann, Thilo Wenzel, Anita Durst, Michael Müller, Ralf Ignatius
European Journal of Clinical Microbiology & Infectious Diseases.2019; 38(4): 719. CrossRef
Enterobius vermicularis infection: prevalence and risk factors among preschool children in kindergarten in the capital area, Republic of the Marshall Islands
Chia-Kwung Fan, Ting-Wu Chuang, Ying-Chieh Huang, Ai-Wen Yin, Chia-Mei Chou, Yu-Ting Hsu, Ramson Kios, Shao-Lun Hsu, Ying-Ting Wang, Mai-Szu Wu, Jia-Wei Lin, Kennar Briand, Chia-Ying Tu
BMC Infectious Diseases.2019;[Epub] CrossRef
Status of common parasitic diseases in Korea in 2019
Sun Huh
Journal of the Korean Medical Association.2019; 62(8): 437. CrossRef
Enterobius vermicularis infection and its risk factors among pre-school children in Taipei, Taiwan
Kuang-Yao Chen, Chuan-Min Yen, Kao-Pin Hwang, Lian-Chen Wang
Journal of Microbiology, Immunology and Infection.2018; 51(4): 559. CrossRef
Prevalence ofEnterobius vermicularisamong preschool children in 2003 and 2013 in Xinxiang city, Henan province, Central China
Shuai Wang, Zhijun Yao, Yichen Hou, Dong Wang, Haizhu Zhang, Jingbo Ma, Luwen Zhang, Shiguo Liu
Parasite.2016; 23: 30. CrossRef
Risk factors for Enterobius vermicularis infection in children in Gaozhou, Guangdong, China
Hong-Mei Li, Chang-Hai Zhou, Zhi-Shi Li, Zhuo-Hui Deng, Cai-Wen Ruan, Qi-Ming Zhang, Ting-Jun Zhu, Long-Qi Xu, Ying-Dan Chen
Infectious Diseases of Poverty.2015;[Epub] CrossRef
High Prevalence of <i>Enterobius vermicularis</i> Infection among Schoolchildren in Three Townships around Yangon, Myanmar
Jong-Yil Chai, Seung Koo Yang, Jae Won Kim, Soo-Lyoen Choi, Gyu-Young Song, Bong-Kwang Jung, Min-Jae Kim, Jaeeun Cho, Deok-Gyu Kim, Woon-Mok Sohn, Hoo-Gn Jeoung, Seon Cho, Jong-Bok Park, Sooji Hong, Thi Thi Htoon, Htay Htay Tin
The Korean Journal of Parasitology.2015; 53(6): 771. CrossRef
Clinical Update on Parasitic Diseases
Min Seo
Korean Journal of Medicine.2013; 85(5): 469. CrossRef

10,345 View
144 Download
Crossref

Trichinella spiralis Infection Suppressed Gut Inflammation with CD4⁺CD25⁺Foxp3⁺ T Cell Recruitment: Min Kyoung Cho, Mi Kyung Park, Shin Ae Kang, Seon Hee Choi, Soon Cheol Ahn, Hak Sun Yu; Korean J Parasitol 2012;50(4):385-390.
Published online November 26, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.4.385

Abstract
PDF

In order to know the effect of pre-existing Trichinella spiralis infection on experimentally induced intestinal inflammation and immune responses, we induced colitis in T. spiralis-infected mice and observed the severity of colitis and the levels of Th1, Th2, and regulatory cytokines and recruitment of CD4⁺CD25⁺Foxp3⁺ T (regulatory T; T_reg) cells. Female C57BL/6 mice were infected with 250 muscle larvae; after 4 weeks, induction of experimental colitis was performed using 3% dextran sulfate sodium (DSS). During the induction period, we observed severity of colitis, including weight loss and status of stool, and evaluated the disease activity index (DAI). A significantly low DAI and degree of weight loss were observed in infected mice, compared with uninfected mice. In addition, colon length in infected mice was not contracted, compared with uninfected mice. We also observed a significant increase in production of pro-inflammatory cytokines, IL-6 and IFN-γ, in spleen lymphocytes treated with DSS; however, such an increase was not observed in infected mice treated with DSS. Of particular interest, production of regulatory cytokines, IL-10 and transforming growth factor (TGF)-β, in spleen lymphocytes showed a significant increase in mice infected with T. spiralis. A similar result was observed in mesenteric lymph nodes (MLN). Subsets of the population of T_reg cells in MLN and spleen showed significant increases in mice infected with T. spiralis. In conclusion, T. spiralis infection can inhibit the DSS-induced colitis in mice by enhancing the regulatory cytokine and T_reg cells recruitment.

Citations

Citations to this article as recorded by

Helminths in alternative therapeutics of inflammatory bowel disease
Himani Pandey, Daryl W. T. Tang, Sunny H. Wong, Devi Lal
Intestinal Research.2025; 23(1): 8. CrossRef
Cathepsin L of Fasciola hepatica meliorates colitis by altering the gut microbiome and inflammatory macrophages
Jing-Zhi Gong, Jun-Jie Huang, Ming Pan, Qi-Wang Jin, Yi-Min Fan, Wen-Qian Shi, Si-Yang Huang
International Journal of Biological Macromolecules.2025; 286: 138270. CrossRef
Improved antiparasitic effects of mebendazole using chitosan and zinc oxide nanocomposites for drug delivery in Trichinella spiralis infected mice during the muscular phase
Nabila M. Mira, Aya M. Henaish, Eman A. Moussa, Ibrahim B. Helal, Shaimaa M. Kasem
Acta Tropica.2025; 263: 107565. CrossRef
Therapeutic potentials of Trichinella spiralis in immune disorders: From allergy to autoimmunity
Minkyoung Cho, Hak Sun Yu
Parasites, Hosts and Diseases.2025; 63(2): 123. CrossRef
Trichinella-derived protein ameliorates colitis by altering the gut microbiome and improving intestinal barrier function
Shao Rong Long, Wen Xuan Shang, Hui Ran Zhang, Miao Jiang, Jing Jing Wang, Ruo Dan Liu, Zhong Quan Wang, Jing Cui, Hualei Sun
International Immunopharmacology.2024; 127: 111320. CrossRef
The gut microbiota is essential for Trichinella spiralis—evoked suppression of colitis
Hualei Sun, Shao Rong Long, Miao Jiang, Hui Ran Zhang, Jing Jing Wang, Zi Xuan Liao, Jing Cui, Zhong Quan Wang, Subbaraman Babu
PLOS Neglected Tropical Diseases.2024; 18(11): e0012645. CrossRef
The Underrated Gut Microbiota Helminths, Bacteriophages, Fungi, and Archaea
Maria Jose Garcia-Bonete, Anandi Rajan, Francesco Suriano, Elena Layunta
Life.2023; 13(8): 1765. CrossRef
Helminth-derived biomacromolecules as therapeutic agents for treating inflammatory and infectious diseases: What lessons do we get from recent findings?
Pritha Chakraborty, Vivekanandhan Aravindhan, Suprabhat Mukherjee
International Journal of Biological Macromolecules.2023; 241: 124649. CrossRef
Potential of human helminth therapy for resolution of inflammatory bowel disease: The future ahead
Toshio Arai, Fernando Lopes
Experimental Parasitology.2022; 232: 108189. CrossRef
Trichinella-induced immunomodulation: Another tale of helminth success
F. Bruschi, D.S. Ashour, A.A. Othman
Food and Waterborne Parasitology.2022; 27: e00164. CrossRef
Trichinella Infection Ameliorated Vincristine-Induced Neuroinflammation in Mice
Young Rae Jo, Hwan Tae Park, Hak Sun Yu, Hyun-Hee Kong
The Korean Journal of Parasitology.2022; 60(4): 247. CrossRef
Immune Protection of a Helminth Protein in the DSS-Induced Colitis Model in Mice
Shao Rong Long, Ruo Dan Liu, Deepak Vijaya Kumar, Zhong Quan Wang, Chien-Wen Su
Frontiers in Immunology.2021;[Epub] CrossRef
Trichinella spiralis:inflammation modulator
Jing Ding, Xiaolei Liu, Xue Bai, Yang Wang, Jian Li, Chun Wang, Shicun Li, Mingyuan Liu, Xuelin Wang
Journal of Helminthology.2020;[Epub] CrossRef
Infection with Toxocara canis Inhibits the Production of IgE Antibodies to α-Gal in Humans: Towards a Conceptual Framework of the Hygiene Hypothesis?
Adnan Hodžić, Lourdes Mateos-Hernández, Emilie Fréalle, Patricia Román-Carrasco, Pilar Alberdi, Muriel Pichavant, Veronica Risco-Castillo, Delphine Le Roux, Jérôme Vicogne, Wolfgang Hemmer, Herbert Auer, Ines Swoboda, Georg Duscher, José de la Fuente, Ale
Vaccines.2020; 8(2): 167. CrossRef
Exposure time determines the protective effect of Trichinella spiralis on experimental colitis
Wenxiao Zheng, Zhenrong Ma, Xi Sun, Yehong Huang, Bin Lu, Xiaogang Chen, Xiang Xue, Xuexian Yang, Xiang Wu
Microbial Pathogenesis.2020; 147: 104263. CrossRef
Effect of Trichinella spp. or derived antigens on chemically induced inflammatory bowel disease (IBD) in mouse models: A systematic review and meta-analysis
Jian Li, Xiaolei Liu, Jing Ding, Bin Tang, Xue Bai, Yang Wang, Shicun Li, Mingyuan Liu, Xuelin Wang
International Immunopharmacology.2020; 85: 106646. CrossRef
Adoptive transfer of Trichinella spiralis-activated macrophages can ameliorate both Th1- and Th2-activated inflammation in murine models
Shin Ae Kang, Mi-Kyung Park, Sang Kyun Park, Jun Ho Choi, Da In Lee, So Myong Song, Hak Sun Yu
Scientific Reports.2019;[Epub] CrossRef
Acute infection with Strongyloides venezuelensis increases intestine production IL-10, reduces Th1/Th2/Th17 induction in colon and attenuates Dextran Sulfate Sodium-induced colitis in BALB/c mice
Vanessa Fernandes Rodrigues, Márcia Paulliny Soares Bahia, Núbia Rangel Cândido, João Marcelo Peixoto Moreira, Vinicius Gustavo Oliveira, Emília Souza Araújo, Jailza Lima Rodrigues Oliveira, Michelle de Carvalho Rezende, Ary Correa, Deborah Negrão-Corrêa
Cytokine.2018; 111: 72. CrossRef
Regulatory T Cell and Forkhead Box Protein 3 as Modulators of Immune Homeostasis
Leonn Mendes Soares Pereira, Samara Tatielle Monteiro Gomes, Ricardo Ishak, Antonio Carlos Rosário Vallinoto
Frontiers in Immunology.2017;[Epub] CrossRef
Ascaris suum infection modulates inflammation: Implication of CD4+ CD25high Foxp3+ T cells and IL‐10
T. de O. Titz, C. A. A. de Araújo, C. S. Enobe, P. O. Rigato, T. M. Oshiro, M. F. de Macedo‐Soares
Parasite Immunology.2017;[Epub] CrossRef
Biodegradable Chitosan Decreases the Immune Response to Trichinella spiralis in Mice
Klaudia Brodaczewska, Natalia Wolaniuk, Katarzyna Lewandowska, Katarzyna Donskow-Łysoniewska, Maria Doligalska
Molecules.2017; 22(11): 2008. CrossRef
Activation and Recruitment of Regulatory T Cells via Chemokine Receptor Activation in Trichinella spiralis-Infected Mice
Jeong-Bin Ahn, Shin Ae Kang, Dong-Hee Kim, Hak Sun Yu
The Korean Journal of Parasitology.2016; 54(2): 163. CrossRef
Regulatory parameters of the lung immune response during the early phase of experimental trichinellosis
Guido H. Falduto, Cecilia C. Vila, María P. Saracino, María V. Gentilini, Stella M. Venturiello
Veterinary Parasitology.2016; 231: 47. CrossRef
Helminth Regulation of Immunity
Fernando Lopes, Chelsea Matisz, José L. Reyes, Humberto Jijon, Ahmed Al-Darmaki, Gilaad G. Kaplan, Derek M. McKay
Inflammatory Bowel Diseases.2016; 22(10): 2499. CrossRef
Immunomodulatory potential of particular Trichinella spiralis muscle larvae excretory–secretory components
J. Cvetkovic, Lj. Sofronic-Milosavljevic, N. Ilic, M. Gnjatovic, I. Nagano, A. Gruden-Movsesijan
International Journal for Parasitology.2016; 46(13-14): 833. CrossRef
Allergenicity of two Anisakis simplex allergens evaluated in vivo using an experimental mouse model
Min Kyoung Cho, Mi Kyung Park, Shin Ae Kang, Maria Luisa Caballero, Teresa Perez-Pinar, Rosa Rodriguez-Perez, Mee Sun Ock, Hee Jae Cha, Yeon Chul Hong, Hak Sun Yu
Experimental Parasitology.2014; 146: 71. CrossRef
Excretory/Secretory Products from Trichinella spiralis Adult Worms Ameliorate DSS-Induced Colitis in Mice
Xiaodi Yang, Yaping Yang, Yunyun Wang, Bin Zhan, Yuan Gu, Yuli Cheng, Xinping Zhu, Udai P. Singh
PLoS ONE.2014; 9(5): e96454. CrossRef
Trichinella spiralisimmunomodulation: an interactive multifactorial process
Dalia S Ashour
Expert Review of Clinical Immunology.2013; 9(7): 669. CrossRef

10,037 View
94 Download
Crossref

Original Articles

A 24 kDa Excretory-Secretory Protein of Anisakis simplex Larvae Could Elicit Allergic Airway Inflammation in Mice: Hye-Kyung Park, Min Kyoung Cho, Mi Kyung Park, Shin Ae Kang, Yun Seong Kim, Ki Uk Kim, Min Ki Lee, Mee Sun Ock, Hee Jae Cha, Hak Sun Yu; Korean J Parasitol 2011;49(4):373-380.
Published online December 16, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.4.373

Abstract
PDF

We have reported that a 24 kDa protein (22U homologous; As22U) of Anisakis simplex larvae could elicit several Th2-related chemokine gene expressions in the intestinal epithelial cell line which means that As22U may play a role as an allergen. In order to determine the contribution of As22U to allergic reactions, we treated mice with 6 times intra-nasal application of recombinant As22U (rAs22U). In the group challenged with rAs22U and ovalbumin (OVA), the number of eosinophils in the bronchial alveolar lavage fluid (BALF) was significantly increased, as compared to the group receiving only OVA. In addition, mice treated with rAs22U and OVA showed significantly increased airway hyperresponsiveness. Thus, severe inflammation around the airway and immune cell recruitment was observed in mice treated with rAs22U plus OVA. The levels of IL-4, IL-5, and IL-13 cytokines in the BALF increased significantly after treatment with rAs22U and OVA. Similarly, the levels of anti-OVA specific IgE and IgG1 increased in mice treated with rAs22U and OVA, compared to those treated only with OVA. The Gro-α (CXCL1) gene expression in mouse lung epithelial cells increased instantly after treatment with rAs22U, and allergy-specific chemokines eotaxin (CCL11) and thymus-and-activation-regulated-chemokine (CCL17) gene expressions significantly increased at 6 hr after treatment. In conclusion, rAs22U may induce airway allergic inflammation, as the result of enhanced Th2 and Th17 responses.

Citations

Citations to this article as recorded by

Anisakis pegreffii Extract Induces Airway Inflammation with Airway Remodeling in a Murine Model System
Jun Ho Choi, Ju Yeong Kim, Myung-hee Yi, Myungjun Kim, Tai-Soon Yong, Kalman Imre
BioMed Research International.2021;[Epub] CrossRef
Allergen-like Molecules from Parasites
Ju Yeong Kim, Myung-Hee Yi, Tai-Soon Yong
Current Protein & Peptide Science.2020; 21(2): 186. CrossRef
Research Note. Prevalence, protein analysis and possible preventive measures against zoonotic anisakid larvae isolated from marine Atherina fish
M. Samir, M. A. Amin, A. O. Hassan, A. M. Merwad, M. A. I. Awadallah
Helminthologia.2015; 52(4): 375. CrossRef
Allergenicity of two Anisakis simplex allergens evaluated in vivo using an experimental mouse model
Min Kyoung Cho, Mi Kyung Park, Shin Ae Kang, Maria Luisa Caballero, Teresa Perez-Pinar, Rosa Rodriguez-Perez, Mee Sun Ock, Hee Jae Cha, Yeon Chul Hong, Hak Sun Yu
Experimental Parasitology.2014; 146: 71. CrossRef
Alteration of Cytokine Production during Visceral Larva Migrans by Toxascaris leonina in Mice
Shin Ae Kang, Mi-Kyung Park, Min Kyoung Cho, Hak Sun Yu
The Korean Journal of Parasitology.2013; 51(5): 583. CrossRef

9,359 View
76 Download
Crossref

Parasitic Helminth Cystatin Inhibits DSS-Induced Intestinal Inflammation Via IL-10⁺F4/80⁺ Macrophage Recruitment: Sung Won Jang, Min Kyoung Cho, Mi Kyung Park, Shin Ae Kang, Byoung-Kuk Na, Soon Cheol Ahn, Dong-Hee Kim, Hak Sun Yu; Korean J Parasitol 2011;49(3):245-254.
Published online September 30, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.3.245

Abstract
PDF

Many immune down-regulatory molecules have been isolated from parasites, including cystatin (cystain protease inhibitor). In a previous study, we isolated and characterized Type I cystatin (CsStefin-1) of the liver fluke, Clonorchis sinensis. To investigate whether the CsStefin-1 might be a new host immune modulator, we induced intestinal inflammation in mice by dextran sodium sulfate (DSS) and treated them with recombinant CsStefin-1 (rCsStefin-1). The disease activity index (DAI) increased in DSS only-treated mice. In contrast, the DAI value was significantly reduced in rCsStefin-1-treated mice than DSS only-treated mice. In addition, the colon length of DSS only-treated mice was shorter than that of rCsStefin-1 treated mice. The secretion levels of IFN-γ and TNF-α in the spleen and mesenteric lymph nodes (MLNs) were significantly increased by DSS treatment, but the level of TNF-α in MLNs was significantly decreased by rCsStefin-1 treatment. IL-10 production in both spleen and MLNs was significantly increased, and IL-10⁺F4/80⁺ macrophage cells were significantly increased in the spleen and MLNs of rCsStefin-1 treated mice after DSS treatment. In conclusion, rCsStefin-1 could reduce the intestinal inflammation occurring after DSS treatment, these effects might be related with recruitment of IL-10 secreting macrophages.

Citations

Citations to this article as recorded by

Schistosoma japonicum cystatin attenuated CLP-induced sepsis in mice though inducing tolerogenic dendritic cells and regulatory T cells
Yi-Xiang Wang, Yi-Dan Ma, Hui-Hui Li, Wen-Juan Duo, Qi-Wang Jin, Kai-Jun Zhou, Yan-Ran Gao, Jun-Nan He, Yu-Jie Xie, Liang Chu, Xiao-Di Yang
Comparative Immunology, Microbiology and Infectious Diseases.2025; 120: 102345. CrossRef
PD-1-dependent therapeutic effect of Trichinella spiralis cystatin on myocardial infarction in a mice model
Weixiao Zhang, Wenhui Yin, Hongtao Wang, Lingqin Wu, Chang Li, Xinyu Peng, Xiang Li, Kaibo Jiang, Huiqi Yang, Chenyue Dang, Erhe Gao, Qiwang Jin, Xiaodi Yang
Parasites & Vectors.2025;[Epub] CrossRef
Amblyostatin-1, the first salivary cystatin with host immunomodulatory and anti-inflammatory properties from the Neotropical tick Amblyomma sculptum, vector of Brazilian spotted fever
Wilson Santos Molari, Mohamed Amine Jmel, Josiane Betim Assis, Alan Frazão-Silva, Júlia Moura Bernardi, Gretta Huamanrayme, José María Medina, Eliane Esteves, Solange Cristina Antão, Gabriel Cerqueira Alves Costa, Aparecida Sadae Tanaka, Andréa Cristina F
Frontiers in Immunology.2025;[Epub] CrossRef
A potential role of protein extractions from Metagonimus yokogawai in amelionating inflammation in patients with ankylosing spondylitis
Eun Jeong Won, Yu Jeong Lee, Moon-Ju Kim, Hae-In Lee, Hyun Hee Jang, Seong Hoon Kim, Hee Min Yoo, Namki Cho, Seung Cheol Shim, Tae-Jong Kim
Experimental Parasitology.2024; 259: 108718. CrossRef
Helminth-derived proteins as immune system regulators: a systematic review of their promise in alleviating colitis
Maimonah Alghanmi, Faisal Minshawi, Tarfa A. Altorki, Ayat Zawawi, Isra Alsaady, Abdallah Y Naser, Hassan Alwafi, Soa’ad M. Alsulami, Ala A. Azhari, Anwar M Hashem, Rowa Alhabbab
BMC Immunology.2024;[Epub] CrossRef
Protection from T cell-dependent colitis by the helminth-derived immunomodulatory mimic of transforming growth factor-β,Hp-TGM
Danielle J Smyth, Madeleine P J White, Chris J C Johnston, Anne-Marie Donachie, Marta Campillo Poveda, Henry J McSorley, Rick M Maizels
Discovery Immunology.2023;[Epub] CrossRef
Bioinformatics analysis and prokaryotic expression of a cystatin analogue from Spirometra erinaceieuropaei
Lin Huang, Ling Mai, Gang Lv, Xinjun Chen
Biotechnology & Biotechnological Equipment.2023;[Epub] CrossRef
Potential of human helminth therapy for resolution of inflammatory bowel disease: The future ahead
Toshio Arai, Fernando Lopes
Experimental Parasitology.2022; 232: 108189. CrossRef
Parasitomimetics: Can We Utilize Parasite-Derived Immunomodulatory Molecules for Interventions to Immunological Disorders?
Kazuki Nagai, Yasuyuki Goto
Frontiers in Immunology.2022;[Epub] CrossRef
Molecular Characteristics and Potent Immunomodulatory Activity of Fasciola hepatica Cystatin
Kai Zhang, Yucheng Liu, Guowu Zhang, Xifeng Wang, Zhiyuan Li, Yunxia Shang, Chengcheng Ning, Chunhui Ji, Xuepeng Cai, Xianzhu Xia, Jun Qiao, Qingling Meng
The Korean Journal of Parasitology.2022; 60(2): 117. CrossRef
Cysteine protease of Clonorchis sinensis alleviates DSS-induced colitis in mice
Xiaoying Xie, Zhanshuai Wu, Yuhong Wu, Jing Liu, Xinyuan Chen, Xiaoqian Shi, Caiheng Wei, Jiasheng Li, Jiahui Lv, Qing Li, Lili Tang, Shanshan He, Tingzheng Zhan, Zeli Tang, John Pius Dalton
PLOS Neglected Tropical Diseases.2022; 16(9): e0010774. CrossRef
Clonorchis sinensis-Derived Protein Attenuates Inflammation and New Bone Formation in Ankylosing Spondylitis
Yu Jeong Lee, Moon-Ju Kim, Sungsin Jo, So-Hee Jin, Pu-Reum Park, Kijeong Park, Ho-Chun Song, Jahae Kim, Ji-Young Kim, Seung Cheol Shim, Tae-Hwan Kim, Sung-Jong Hong, Hyundeok Kang, Tae-Jong Kim, Eun Jeong Won
Frontiers in Immunology.2021;[Epub] CrossRef
Helminth-derived cystatins: the immunomodulatory properties of an Ascaris lumbricoides cystatin
Luis Caraballo, Josefina Zakzuk, Nathalie Acevedo
Parasitology.2021; 148(14): 1744. CrossRef
Effect of cysteine peptidase inhibitor of Eudiplozoon nipponicum (Monogenea) on cytokine expression of macrophages in vitro
Jana Ilgová, Lenka Kavanová, Katarína Matiašková, Jiří Salát, Martin Kašný
Molecular and Biochemical Parasitology.2020; 235: 111248. CrossRef
Amelioration of type 1 diabetes by recombinant fructose-1,6-bisphosphate aldolase and cystatin derived from Schistosoma japonicum in a murine model
Ke Yan, Bo Wang, Huabang Zhou, Qingli Luo, Jilong Shen, Yunxia Xu, Zhengrong Zhong
Parasitology Research.2020; 119(1): 203. CrossRef
Wuchereria bancrofti macrophage migration inhibitory factor‐2 (rWbaMIF‐2) ameliorates experimental colitis
Shriram Ramani, Nikhil Chauhan, Vishal Khatri, Connie Vitali, Ramaswamy Kalyanasundaram
Parasite Immunology.2020;[Epub] CrossRef
Integrated omics profiling of dextran sodium sulfate-induced colitic mice supplemented with Wolfberry (Lycium barbarum)
Wanping Aw, Huijuan Jia, Weida Lyu, Shinji Fukuda, Masaru Tomita, Lila Otani, Hisanori Kato
npj Science of Food.2020;[Epub] CrossRef
Therapeutic efficacy of Schistosoma japonicum cystatin on sepsis-induced cardiomyopathy in a mouse model
Shifang Gao, Huihui Li, Hong Xie, Shili Wu, Yuan Yuan, Liang Chu, Siying Sun, Huijuan Yang, Lingqin Wu, Yongsheng Bai, Qiao Zhou, Xin Wang, Bin Zhan, Hu Cui, Xiaodi Yang
Parasites & Vectors.2020;[Epub] CrossRef
Inflammatory bowel diseases, the hygiene hypothesis and the other side of the microbiota: Parasites and fungi
Simona Panelli, Sara Epis, Lucia Cococcioni, Matteo Perini, Moira Paroni, Claudio Bandi, Lorenzo Drago, Gian Vincenzo Zuccotti
Pharmacological Research.2020; 159: 104962. CrossRef
Parasite Cystatin: Immunomodulatory Molecule with Therapeutic Activity against Immune Mediated Disorders
Vishal Khatri, Nikhil Chauhan, Ramaswamy Kalyanasundaram
Pathogens.2020; 9(6): 431. CrossRef
What Can Parasites Tell Us About the Pathogenesis and Treatment of Asthma and Allergic Diseases
Sina Bohnacker, Fabiana Troisi, Marta de los Reyes Jiménez, Julia Esser-von Bieren
Frontiers in Immunology.2020;[Epub] CrossRef
Adoptive transfer of Trichinella spiralis-activated macrophages can ameliorate both Th1- and Th2-activated inflammation in murine models
Shin Ae Kang, Mi-Kyung Park, Sang Kyun Park, Jun Ho Choi, Da In Lee, So Myong Song, Hak Sun Yu
Scientific Reports.2019;[Epub] CrossRef
Molecular Characterization of a Dirofilaria immitis Cysteine Protease Inhibitor (Cystatin) and Its Possible Role in Filarial Immune Evasion
Xiaowei Dong, Jing Xu, Hongyu Song, Yuchen Liu, Maodi Wu, Haojie Zhang, Bo Jing, Weimin Lai, Xiaobin Gu, Yue Xie, Xuerong Peng, Guangyou Yang
Genes.2019; 10(4): 300. CrossRef
Cystatin from Filarial Parasites Suppress the Clinical Symptoms and Pathology of Experimentally Induced Colitis in Mice by Inducing T-Regulatory Cells, B1-Cells, and Alternatively Activated Macrophages
Nalini Bisht, Vishal Khatri, Nikhil Chauhan, Ramaswamy Kalyanasundaram
Biomedicines.2019; 7(4): 85. CrossRef
SXP–RAL Family Filarial Protein, rWbL2, Prevents Development of DSS-Induced Acute Ulcerative Colitis
Namdev S. Togre, Priyanka S. Bhoj, Vishal K. Khatri, Aditya Tarnekar, Kalyan Goswami, Moreshwar R. Shende, M. V. R. Reddy
Indian Journal of Clinical Biochemistry.2018; 33(3): 282. CrossRef
Structural basis of the cystein protease inhibitor Clonorchis sinensis Stefin-1
So Young Park, Mi Suk Jeong, Seong Ah Park, Sung Chul Ha, Byoung-Kuk Na, Se Bok Jang
Biochemical and Biophysical Research Communications.2018; 498(1): 9. CrossRef
A Role for Epitope Networking in Immunomodulation by Helminths
E. Jane Homan, Robert D. Bremel
Frontiers in Immunology.2018;[Epub] CrossRef
The Untapped Pharmacopeic Potential of Helminths
Janina Kahl, Norbert Brattig, Eva Liebau
Trends in Parasitology.2018; 34(10): 828. CrossRef
The somatic proteins of Toxocara canis larvae and excretory-secretory products revealed by proteomics
Márcia B. da Silva, Juan R. Urrego A., Yisela Oviedo, Philip J. Cooper, Luis G.C. Pacheco, Carina S. Pinheiro, Fátima Ferreira, Peter Briza, Neuza M. Alcantara-Neves
Veterinary Parasitology.2018; 259: 25. CrossRef
Modulation of Host Immunity by Helminths: The Expanding Repertoire of Parasite Effector Molecules
Rick M. Maizels, Hermelijn H. Smits, Henry J. McSorley
Immunity.2018; 49(5): 801. CrossRef
Recombinant proteins of helminths with immunoregulatory properties and their possible therapeutic use
Leonardo Nascimento Santos, Luis Gustavo Carvalho Pacheco, Carina Silva Pinheiro, Neuza Maria Alcantara-Neves
Acta Tropica.2017; 166: 202. CrossRef
A recombinant cystatin from Ascaris lumbricoides attenuates inflammation of DSS‐induced colitis
S. Coronado, L. Barrios, J. Zakzuk, R. Regino, V. Ahumada, L. Franco, Y. Ocampo, L. Caraballo
Parasite Immunology.2017;[Epub] CrossRef
Therapeutic effect of Schistosoma japonicum cystatin on bacterial sepsis in mice
Huihui Li, Shushu Wang, Bin Zhan, Wenxin He, Liang Chu, Dapeng Qiu, Nan Li, Yongkun Wan, Hui Zhang, Xingzhi Chen, Qiang Fang, Jilong Shen, Xiaodi Yang
Parasites & Vectors.2017;[Epub] CrossRef
Elevated expression of CST1 promotes breast cancer progression and predicts a poor prognosis
Da-nian Dai, Yan Li, Bo Chen, Yong Du, Shi-bing Li, Shi-xun Lu, Zhi-ping Zhao, Ai-jun Zhou, Ning Xue, Tian-liang Xia, Mu-sheng Zeng, Qian Zhong, Wei-dong Wei
Journal of Molecular Medicine.2017; 95(8): 873. CrossRef
Parasite-Derived Proteins for the Treatment of Allergies and Autoimmune Diseases
Zhenyu Wu, Lifu Wang, Yanlai Tang, Xi Sun
Frontiers in Microbiology.2017;[Epub] CrossRef
Helminths in the gastrointestinal tract as modulators of immunity and pathology
Fumi Varyani, John O. Fleming, Rick M. Maizels
American Journal of Physiology-Gastrointestinal and Liver Physiology.2017; 312(6): G537. CrossRef
Immuno-Modulatory Effect and Therapeutic Potential of Brugia malayi Cystatin in Experimentally Induced Arthritis
Ravi Shankar Prasad Yadav, Vishal Khatri, Nitin Amdare, Kalyan Goswami, V. B. Shivkumar, Nitin Gangane, Maryada Venkata Rami Reddy
Indian Journal of Clinical Biochemistry.2016; 31(2): 203. CrossRef
Therapeutic potential of recombinant cystatin from Schistosoma japonicum in TNBS-induced experimental colitis of mice
Shushu Wang, Yuanyuan Xie, Xiaodi Yang, Xuesong Wang, Ke Yan, Zhengrong Zhong, Xiaowei Wang, Yuanhong Xu, Yi Zhang, Fang Liu, Jilong Shen
Parasites & Vectors.2016;[Epub] CrossRef
Helminth Regulation of Immunity
Fernando Lopes, Chelsea Matisz, José L. Reyes, Humberto Jijon, Ahmed Al-Darmaki, Gilaad G. Kaplan, Derek M. McKay
Inflammatory Bowel Diseases.2016; 22(10): 2499. CrossRef
Unraveling the Hygiene Hypothesis of helminthes and autoimmunity: origins, pathophysiology, and clinical applications
Mathilde Versini, Pierre-Yves Jeandel, Tomer Bashi, Giorgia Bizzaro, Miri Blank, Yehuda Shoenfeld
BMC Medicine.2015;[Epub] CrossRef
TLR2‐dependent amelioration of allergic airway inflammation by parasitic nematode type II MIF in mice
M. K. Cho, M. K. Park, S. A. Kang, S. K. Park, J. H. Lyu, D.‐H. Kim, H.‐K. Park, H. S. Yu
Parasite Immunology.2015; 37(4): 180. CrossRef
Brugia malayi cystatin therapeutically ameliorates dextran sulfate sodium‐induced colitis in mice
Vishal Khatri, Nitin Amdare, Aaditya Tarnekar, Kalyan Goswami, Maryada Venkata Rami Reddy
Journal of Digestive Diseases.2015; 16(10): 585. CrossRef
Generating a detailed protein profile of Fasciola hepatica during the chronic stage of infection in cattle
Orçun Haçarız, Ahmet Tarık Baykal, Mete Akgün, Pınar Kavak, Mahmut Şamil Sağıroğlu, Gearóid Patrick Sayers
PROTEOMICS.2014; 14(12): 1519. CrossRef
Cloning, expression and characterisation of a type II cystatin from Schistosoma japonicum, which could regulate macrophage activation
Xiao Yang, Ju Liu, Yuan Yue, Wei Chen, Man Song, Ximei Zhan, Zhongkai Wu
Parasitology Research.2014; 113(11): 3985. CrossRef
Identification and characterization of the second cysteine protease inhibitor of Clonorchis sinensis (CsStefin-2)
Jung-Mi Kang, Hye-Lim Ju, Kon Ho Lee, Tong-Soo Kim, Jhang Ho Pak, Woon-Mok Sohn, Byoung-Kuk Na
Parasitology Research.2014; 113(1): 47. CrossRef
Disequilibrium of M1 and M2 Macrophages Correlates with the Development of Experimental Inflammatory Bowel Diseases
Wei Zhu, Jianbo Yu, Ying Nie, XueKui Shi, Yang Liu, Fujuan Li, Xiao-li Zhang
Immunological Investigations.2014; 43(7): 638. CrossRef
Of worms, mice and man: An overview of experimental and clinical helminth-based therapy for inflammatory bowel disease
Marthe Heylen, Nathalie E. Ruyssers, Els M. Gielis, Els Vanhomwegen, Paul A. Pelckmans, Tom G. Moreels, Joris G. De Man, Benedicte Y. De Winter
Pharmacology & Therapeutics.2014; 143(2): 153. CrossRef
Worm Proteins of Schistosoma mansoni Reduce the Severity of Experimental Chronic Colitis in Mice by Suppressing Colonic Proinflammatory Immune Responses
Marthe Heylen, Nathalie E. Ruyssers, Joris G. De Man, Jean-Pierre Timmermans, Paul A. Pelckmans, Tom G. Moreels, Benedicte Y. De Winter, Benoit Foligne
PLoS ONE.2014; 9(10): e110002. CrossRef
Immunomodulation by helminth parasites: Defining mechanisms and mediators
Henry J. McSorley, James P. Hewitson, Rick M. Maizels
International Journal for Parasitology.2013; 43(3-4): 301. CrossRef
Helminth Infections and Host Immune Regulation
Henry J. McSorley, Rick M. Maizels
Clinical Microbiology Reviews.2012; 25(4): 585. CrossRef

11,987 View
122 Download
Crossref

Protease-Activated Receptor 2 Is Involved in Th2 Responses against Trichinella spiralis Infection: Mi Kyung Park, Min Kyoung Cho, Shin Ae Kang, Hye-Kyung Park, Yun Seong Kim, Ki Uk Kim, Soon Cheol Ahn, Dong-Hee Kim, Hak Sun Yu; Korean J Parasitol 2011;49(3):235-243.
Published online September 30, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.3.235

Abstract
PDF

In order to get a better understanding of the role of protease-activated receptor 2 (PAR2) in type 2 helper T (Th2) cell responses against Trichinella spiralis infection, we analyzed Th2 responses in T. spiralis-infected PAR2 knockout (KO) mice. The levels of the Th2 cell-secreted cytokines, IL-4, IL-5, and IL-13 were markedly reduced in the PAR2 KO mice as compared to the wild type mice following infection with T. spiralis. The serum levels of parasite-specific IgE increased significantly in the wild type mice as the result of T. spiralis infection, but this level was not significantly increased in PAR2 KO mice. The expression level of thymic stromal lymphopoietin, IL-25, and eotaxin gene (the genes were recently known as Th2 response initiators) of mouse intestinal epithelial cells were increased as the result of treatment with T. spiralis excretory-secretory proteins. However, the expression of these chemokine genes was inhibited by protease inhibitor treatments. In conclusion, PAR2 might involve in Th2 responses against T. spiralis infection.

Citations

Citations to this article as recorded by

Innate Immune Recognition of Helminths: TLRs and Beyond
Camila de Almeida Lopes, Linda Djune Yemeli, Pedro Gazzinelli‐Guimaraes
Parasite Immunology.2025;[Epub] CrossRef
A novel trypsin of Trichinella spiralis mediates larval invasion of gut epithelium via binding to PAR2 and activating ERK1/2 pathway
Lu Lu Han, Qi Qi Lu, Wen Wen Zheng, Yang Li Li, Yan Yan Song, Xin Zhuo Zhang, Shao Rong Long, Ruo Dan Liu, Zhong Quan Wang, Jing Cui, Krystyna Cwiklinski
PLOS Neglected Tropical Diseases.2024; 18(1): e0011874. CrossRef
Pathogenesis ofBrucellaepididymoorchitis-game ofBrucelladeath
Jiuwang Yu, Sha Li, Lu Wang, Zhiheng Dong, Lengge Si, Lidao Bao, Lan Wu
Critical Reviews in Microbiology.2022; 48(1): 96. CrossRef
Cytokines and beyond: Regulation of innate immune responses during helminth infection
Oyebola O. Oyesola, Simon P. Früh, Lauren M. Webb, Elia D. Tait Wojno
Cytokine.2020; 133: 154527. CrossRef
Animal Models for Functional Gastrointestinal Disorders
Alison Accarie, Tim Vanuytsel
Frontiers in Psychiatry.2020;[Epub] CrossRef
Adoptive transfer of Trichinella spiralis-activated macrophages can ameliorate both Th1- and Th2-activated inflammation in murine models
Shin Ae Kang, Mi-Kyung Park, Sang Kyun Park, Jun Ho Choi, Da In Lee, So Myong Song, Hak Sun Yu
Scientific Reports.2019;[Epub] CrossRef
The role of rare innate immune cells in Type 2 immune activation against parasitic helminths
LAUREN M. WEBB, ELIA D. TAIT WOJNO
Parasitology.2017; 144(10): 1288. CrossRef
The induction of the collagen capsule synthesis by Trichinella spiralis is closely related to protease-activated receptor 2
Mi Kyung Park, Min Kyoung Cho, Shin Ae Kang, Bo Young Kim, Hak Sun Yu
Veterinary Parasitology.2016; 230: 56. CrossRef
Human Helminths and Allergic Disease: The Hygiene Hypothesis and Beyond
Thomas B. Nutman, Helton C. Santiago
The American Journal of Tropical Medicine and Hygiene.2016; 95(4): 746. CrossRef
The role of infection in the pathogenesis of allergodermatoses
E. V. Svirshchevskaya, E. V. Matushevskaya, D. B. Chudakov, Yu. I. Matushevskaya
Klinicheskaya dermatologiya i venerologiya.2015; 14(2): 4. CrossRef
Genetic regulation of immunoglobulin E level in different pathological states: integration of mouse and human genetics
Elena S. Gusareva, Iryna Kurey, Igor Grekov, Marie Lipoldová
Biological Reviews.2014; 89(2): 375. CrossRef
Thymic stromal lymphopoietin and atopic diseases
J.M. Leyva-Castillo, M. Li
Revue Française d'Allergologie.2014; 54(5): 364. CrossRef
Parasitic Nematode-Induced CD4+Foxp3+T Cells Can Ameliorate Allergic Airway Inflammation
Shin Ae Kang, Mi-Kyung Park, Min Kyoung Cho, Sang Kyun Park, Min Seong Jang, Bo-Gie Yang, Myoung Ho Jang, Dong-Hee Kim, Hak Sun Yu, Edward Mitre
PLoS Neglected Tropical Diseases.2014; 8(12): e3410. CrossRef
Coinfection with Clonorchis sinensis modulates murine host response against Trichinella spiralis infection
Ying Chen, Bo Huang, Shiguang Huang, Xinbing Yu, Yonglong Li, Wenjian Song, Yongxiang Li, Fangli Lu
Parasitology Research.2013; 112(9): 3167. CrossRef
Alteration of Cytokine Production during Visceral Larva Migrans by Toxascaris leonina in Mice
Shin Ae Kang, Mi-Kyung Park, Min Kyoung Cho, Hak Sun Yu
The Korean Journal of Parasitology.2013; 51(5): 583. CrossRef
Toward Drugs for Protease-Activated Receptor 2 (PAR2)
Mei-Kwan Yau, Ligong Liu, David P. Fairlie
Journal of Medicinal Chemistry.2013; 56(19): 7477. CrossRef
IL-32-PAR2 axis is an innate immunity sensor providing alternative signaling for LPS-TRIF axis
Masanori Nakayama, Yasuo Niki, Toshiki Kawasaki, Yuki Takeda, Hiroyasu Ikegami, Yoshiaki Toyama, Takeshi Miyamoto
Scientific Reports.2013;[Epub] CrossRef
Protease‐activated receptor‐2 signalling by tissue factor on dendritic cells suppresses antigen‐specific CD4+ T‐cell priming
Seema Shrivastava, Liang Ma, El‐Li Tham, John H. McVey, Daxin Chen, Anthony Dorling
Immunology.2013; 139(2): 219. CrossRef
Molecular characterization of 45kDa aspartic protease of Trichinella spiralis
Jong Nam Park, Sang Kyun Park, Min Kyoung Cho, Mi-Kyung Park, Shin Ae Kang, Dong-Hee Kim, Hak Sun Yu
Veterinary Parasitology.2012; 190(3-4): 510. CrossRef
Regulation and dysregulation of innate immunity by NFAT signaling downstream of pattern recognition receptors (PRRs)
Ivan Zanoni, Francesca Granucci
European Journal of Immunology.2012; 42(8): 1924. CrossRef
TSLP Expression: Cellular Sources, Triggers, and Regulatory Mechanisms
Toshiro Takai
Allergology International.2012; 61(1): 3. CrossRef
Regulation of epithelial immunity by IL-17 family cytokines
Rajita Pappu, Sascha Rutz, Wenjun Ouyang
Trends in Immunology.2012; 33(7): 343. CrossRef
Regulation of type 2 immunity to helminths by mast cells
Matthew R. Hepworth, Marcus Maurer, Susanne Hartmann
Gut Microbes.2012; 3(5): 476. CrossRef

10,302 View
86 Download
Crossref

Seroprevalence of Antibodies against Anisakis simplex Larvae among Health-Examined Residents in Three Hospitals of Southern Parts of Korea: Jung Kim, Jin Ok Jo, Seon Hee Choi, Min Kyoung Cho, Hak Sun Yu, Hee Jae Cha, Meesun Ock; Korean J Parasitol 2011;49(2):139-144.
Published online June 14, 2011; DOI: https://doi.org/10.3347/kjp.2011.49.2.139

Abstract
PDF

The present study was performed to estimate the seroprevalence of larval Anisakis simplex infection among the residents health-examined in 3 hospitals in southern parts of Korea. A total of 498 serum samples (1 serum per person) were collected in 3 hospitals in Busan Metropolitan city, Masan city, and Geoje city in Gyeongsangnam-do (Province) and were examined by IgE-ELISA and IgE-western blotting with larval A. simplex crude extract and excretory-secretory products (ESP). The prevalence of antibody positivity was 5.0% and 6.6% with ELISA against crude extracts and ESP, respectively. It was also revealed that infection occurred throughout all age groups and higher in females than in males. A specific protein band of 130 kDa was detected from 10 patients with western blot analysis against crude extract and ESP among those who showed positive results by ELISA. Our study showed for the first time the seroprevalence of anisakiasis in Korea. The allergen of 130 kDa can be a candidate for serologic diagnosis of anisakiasis.

Citations

Citations to this article as recorded by

Seroprevalence of IgG and IgE Antibodies Against Anisakis in the Presumably Healthy Population of the Canary Islands
Eligia González-Rodríguez, Marta Rodero, J. Alberto Montoya-Alonso, Kevin M. Santana-Hernández, Myriam R. Ventura, Carmen Cuéllar, Eligia Rodríguez-Ponce
Antibodies.2025; 14(3): 60. CrossRef
Proteome identification of common immunological proteins of two nematode parasites
Shin Ae Kang, Hak Sun Yu
Parasites, Hosts and Diseases.2024; 62(3): 342. CrossRef
Progress in Anisakis Allergy Research: Milestones and Reversals
Alvaro Daschner, Carmen Cuéllar
Current Treatment Options in Allergy.2020; 7(4): 457. CrossRef
IgE sensitization to Anisakis pegreffii in Italy: Comparison of two methods for the diagnosis of allergic anisakiasis
S. Mattiucci, A. Colantoni, B. Crisafi, F. Mori‐Ubaldini, L. Caponi, P. Fazii, G. Nascetti, F. Bruschi
Parasite Immunology.2017;[Epub] CrossRef
Anthelmintic Activities of Aporphine from Nelumbo nucifera Gaertn. cv. Rosa-plena against Hymenolepis nana
Rong-Jyh Lin, Mei-Hsuan Wu, Yi-Hsuan Ma, Li-Yu Chung, Chung-Yi Chen, Chuan-Min Yen
International Journal of Molecular Sciences.2014; 15(3): 3624. CrossRef
Endoscopic imaging of parasites in the human digestive tract
Naoki Hosoe, Haruhiko Ogata, Toshifumi Hibi
Parasitology International.2014; 63(1): 216. CrossRef
An Extended Study of Seroprevalence of Anti‐Anisakis simplex IgE Antibodies in Norwegian Blood Donors
A. H. Lin, I. Nepstad, E. Florvaag, E. Egaas, T. Van Do
Scandinavian Journal of Immunology.2014; 79(1): 61. CrossRef
Occurrence of anisakid nematode larvae in chub mackerel (Scomber japonicus) caught off Korea
Tae-Jong Bak, Chan-Hyeok Jeon, Jeong-Ho Kim
International Journal of Food Microbiology.2014; 191: 149. CrossRef
Clinical Characteristics of Gastroallergic Anisakiasis and Diagnostic Implications of Immunologic Tests
Young-Bae Chung, Jaechun Lee
Allergy, Asthma & Immunology Research.2014; 6(3): 228. CrossRef
Preventive and Therapeutic Effects of <italic>Anisakis simplex</italic> Larval Protein in a Mouse Model of Crohn’S Disease
Hee-Jae Cha, Mee Sun Ock
Kosin Medical Journal.2013; 28(2): 107. CrossRef
Cross-sectional study of serum reactivity to Anisakis simplex in healthy adults in Niter?i, Brazil
Israel Junior, Mauricio Vericimo, Luciana Cardoso, Sergio Clemente, Elmiro Nascimento, Gerlinde Teixeira
Acta Parasitologica.2013;[Epub] CrossRef
Detection of Microorganisms in Granulomas That Have Been Formalin-Fixed: Review of the Literature Regarding Use of Molecular Methods
Jeannette Guarner
Scientifica.2012; 2012: 1. CrossRef
Anisakidosis in Korea; Changes Over the Last Decade
Hee Jae Cha, Mee Sun Ock
Kosin Medical Journal.2012; 27(2): 73. CrossRef
Prevalence of Zoonotic Anisakid Nematodes in Inuit-Harvested Fish and Mammals from the Eastern Canadian Arctic
Erica L. Pufall, Andria Jones-Bitton, Scott A. McEwen, Tanya M. Brown, Victoria L. Edge, Jerzy Rokicki, Katarzyna Karpiej, Andrew S. Peregrine, Manon Simard
Foodborne Pathogens and Disease.2012; 9(11): 1002. CrossRef
A technique for the intra-gastric administration of live larvae of Anisakis simplex in mice
Israel Figueiredo, Luciana Cardoso, Gerlinde Teixeira, Leila Lopes, Sergio Carmona São Clemente, Mauricio Afonso Vericimo
Experimental Parasitology.2012; 130(3): 285. CrossRef

9,956 View
92 Download
Crossref

Parents' Knowledge about Enterobiasis Might Be One of the Most Important Risk Factors for Enterobiasis in Children: Dong-Hee Kim, Hyun-Mi Son, Joo Young Kim, Min Kyoung Cho, Mee Kyung Park, Sin Ye Kang, Bo Young Kim, Hak Sun Yu; Korean J Parasitol 2010;48(2):121-126.
Published online June 17, 2010; DOI: https://doi.org/10.3347/kjp.2010.48.2.121

Abstract
PDF

To know the prevalence of Enterobius vermicularis infection and what are the most important risk factors, we evaluated the incidence and risk factors of enterobiasis among children attended in kindergartens in Busan metropolitan city, Republic of Korea. A total of 1,674 children from 21 kindergartens in 11 of 16 autonomous districts of Busan were evaluated for E. vermicularis infection by the cellotape anal swab technique. The overall egg-positive rate for E. vermicularis was 10.7% (179/1,674), and the prevalence of enterobiasis in each kindergarten ranged between 0% and 32.4%. There was an increasing tendency of the egg positive rate according to the population density; the higher the population density communities had, the higher egg-positive rate for E. vermicularis was detected (P = 0.001). Among personal hygiene factors involving children, thumb-sucking (P = 0.036) and fingernail-trimming (P = 0.024) were highly associated with enterobiasis. In addition, taking anthelmintic medications against E. vermicularis infection was strongly associated with enterobiasis (P = 0.014). Moreover, parents' knowledge of enterobiasis was correlated significantly with the incidence of enterobiasis of their children (P = 0.006). In conclusion, we need to consider not only personal hygiene but also parents' knowledge about enterobiasis as a factor in order to develop new strategies for elimination or to complete reduction of enterobiasis in Korea.

Citations

Citations to this article as recorded by

Unveiling the disparities between private and public preschools in the risk for pinworm infections in China: a multicentre cross-sectional study
Fanzhen Mao, Xiangzhen Xu, Feng Tang, Bixian Ni, Qiang Zhang, Jiayao Zhang, Yaobao Liu, You Li, Jun Cao
BMC Public Health.2025;[Epub] CrossRef
Epidemiological and molecular characterization of Enterobius vermicularis cases in Mersin, Turkey: An analysis based on mitochondrial DNA cox1 gene sequences
Harun Gülbudak, Seda Tezcan Ülger, Taylan Bozok, Gönül Aslan
One Health.2025; 20: 101079. CrossRef
Parasitoses intestinales infantiles
P. Bourée
EMC - Traité de médecine AKOS.2025; 28(3): 1. CrossRef
Enterobius vermicularis infection and its risk factors among rural schoolchildren in Zhejiang Province: a cross-sectional study
Jialie Jin, Wei Ruan, Wenjie Xu, Xuan Zhang, Kegen Yu, Hualiang Chen, Jiaqi Zhang, Jimin Sun, Jianmin Jiang, Xiaoxiao Wang
Parasitology Research.2025;[Epub] CrossRef
Pinworm (Enterobius Vermicularis) Infestation: An Updated Review
Alexander K.C. Leung, Joseph M. Lam, Benjamin Barankin, Alex H.C. Wong, Kin F. Leong, Kam L. Hon
Current Pediatric Reviews.2025; 21(4): 333. CrossRef
Parasitosis intestinales en niños
P. Bourée
EMC - Tratado de Medicina.2025; 29(4): 1. CrossRef
Parassitosi intestinali infantili
P. Bourée
EMC - AKOS - Trattato di Medicina.2025; 27(4): 1. CrossRef
Oxyurose, trichocéphalose et ankylostomoses
F. Robert-Gangneux, B. Autier, C. Guiguen
EMC - Maladies infectieuses.2024; 41(3): 1. CrossRef
Global prevalence of enterobiasis in young children over the past 20 years: a systematic review and meta-analysis
Elham Kia Lashaki, Azadeh Mizani, Seyed Abdollah Hosseini, Bentolhoda Habibi, Khadijeh Taherkhani, Amir Javadi, AliReza Taremiha, Samira Dodangeh
Osong Public Health and Research Perspectives.2023; 14(6): 441. CrossRef
Enterobiasis among Yemeni children: a cross-sectional study
Abdulelah H. Al-Adhroey, Yahya A. Al-Ansi, Mohammed A. Al-Kholani, Abdulrahman H. Amer, Marwan M. Al-Khyat, Fadia H. Al Hubaishi, Radhwan H. Aziz, Ebrahim S. Al-Khateeb, Souad A. Al-Gabri, Tawfik M. Al-Gabri
Journal of Parasitic Diseases.2022; 46(3): 722. CrossRef
Prevalence and genetic analysis of Enterobius vermicularis in schoolchildren in lower northern Thailand
Pichamon Janthu, Abdulhakam Dumidae, Chanakan Subkrasae, Jiranun Ardpairin, Saengchai Nateeworanart, Aunchalee Thanwisai, Apichat Vitta
Parasitology Research.2022; 121(10): 2955. CrossRef
Is pinworm infection still a public health concern among children in resource-rich regions? Trends in pinworm infection prevalence and associated factors among children in Hualien County, Taiwan: a retrospective cross-sectional study
Yu-Chao Hsiao, Jen-Hung Wang, Chia-Hsiang Chu, Yu-Hsun Chang, Yung-Chieh Chang, Rong-Hwa Jan, Shao-Yin Chu, Shang-Hsien Yang, Jui-Shia Chen, Ming-Chun Chen
BMC Public Health.2022;[Epub] CrossRef
Enterobius vermicularis Infection among Preschool Children: A 12-Year (2008-2019) Survey in Large Cities and Provinces of the Republic of Korea
Hyejoo Shin, Bong-Kwang Jung, Seungwan Ryoo, Sooji Hong, Taehee Chang, Jiyeon Park, Keon Hoon Lee, Jeonggyu Lee, Jae Young Park, Hoo-Gn Jeoung, Jae Hyun Cho, Jong-Yil Chai
The Korean Journal of Parasitology.2021; 59(4): 421. CrossRef
Prevalencia y variables asociadas a la transmisión de Enterobius vermicularis en niños en edad preescolar y escolar en dos municipios de Cundinamarca, Colombia
Julio Cesar Giraldo Forero, Laura Catalina Rodríguez Fonseca, Lina Maria Pinzón Triana, Valentina Vega Díaz
Revista Med.2020; 27(1): 17. CrossRef
Prevalence of Enterobius vermicularis infections and associated risk factors among schoolchildren in Nakhon Si Thammarat, Thailand
Pokkamol Laoraksawong, Pimyada Pansuwan, Supakrit Krongchon, Pongphan Pongpanitanont, Penchom Janwan
Tropical Medicine and Health.2020;[Epub] CrossRef
Detection of Enterobius vermicularis in greater Berlin, 2007–2017: seasonality and increased frequency of detection
Johannes Friesen, Clara Bergmann, Robert Neuber, Jörg Fuhrmann, Thilo Wenzel, Anita Durst, Michael Müller, Ralf Ignatius
European Journal of Clinical Microbiology & Infectious Diseases.2019; 38(4): 719. CrossRef
Enterobius vermicularis infection and its risk factors among pre-school children in Taipei, Taiwan
Kuang-Yao Chen, Chuan-Min Yen, Kao-Pin Hwang, Lian-Chen Wang
Journal of Microbiology, Immunology and Infection.2018; 51(4): 559. CrossRef
The frequency of Enterobius vermicularis infection in children from Wielkopolska Voivodeship
Marta Siekierska, Karolina Kot, Natalia Łanocha-Arendarczyk, Danuta Kosik-Bogacka
Diagnostyka Laboratoryjna.2018; 54(1): 5. CrossRef
Oxyurose, trichocéphalose et ankylostomoses
C. Guiguen, F. Robert-Gangneux
EMC - Maladies infectieuses.2018; 35(4): 1. CrossRef
Prevalence ofEnterobius vermicularisamong Children in Iran: A Systematic Review and Meta-analysis
Mahmood Moosazadeh, Ghasem Abedi, Mahdi Afshari, Seif Ali Mahdavi, Fereshteh Farshidi, Elham Kheradmand
Osong Public Health and Research Perspectives.2017; 8(2): 108. CrossRef
Prevalence of Enterobius vermicularis Among Children in Kindergartens and Primary Schools in Iran: A Systematic Review and Meta-Analysis
Mahmood Moosazadeh, Ghasem Abedi, Mahdi Afshari, Seif Ali Mahdavi, Fereshteh Farshidi, Elham Kheradmand
Osong Public Health and Research Perspectives.2016;[Epub] CrossRef
New insights ofEnterobius vermicularisinfection among preschool children in an urban area in Malaysia
T. S. Anuar, L. Jalilah, M. Norhayati, M. Y. Azlin, M. S. Fatmah, H. M. Al-Mekhlafi
Helminthologia.2016; 53(1): 76. CrossRef
Prevalence ofEnterobius vermicularisamong preschool children in 2003 and 2013 in Xinxiang city, Henan province, Central China
Shuai Wang, Zhijun Yao, Yichen Hou, Dong Wang, Haizhu Zhang, Jingbo Ma, Luwen Zhang, Shiguo Liu
Parasite.2016; 23: 30. CrossRef
Risk factors for Enterobius vermicularis infection in children in Gaozhou, Guangdong, China
Hong-Mei Li, Chang-Hai Zhou, Zhi-Shi Li, Zhuo-Hui Deng, Cai-Wen Ruan, Qi-Ming Zhang, Ting-Jun Zhu, Long-Qi Xu, Ying-Dan Chen
Infectious Diseases of Poverty.2015;[Epub] CrossRef
Negligible Egg Positive Rate of <i>Enterobius vermicularis</i> and No Detection of Head Lice among Orphanage Children in Busan and Ulsan, Korea (2014)
Dong-Hee Kim, Hyun-Mi Son, Sang Hwa Lee, Mi Kyung Park, Shin Ae Kang, Sang Kyun Park, Jun-Ho Choi, Jung Ha Park, Hak-Sun Yu
The Korean Journal of Parasitology.2015; 53(4): 497. CrossRef
Kindergarten Teacher’s Knowledge of Enterobiasis in Korea
Dong-Hee Kim, Hak-Sun Yu
Open Journal of Nursing.2014; 04(04): 330. CrossRef
Effect of a One-Off Educational Session about Enterobiasis on Knowledge, Preventative Practices, and Infection Rates among Schoolchildren in South Korea
Dong-Hee Kim, Hak Sun Yu, David Joseph Diemert
PLoS ONE.2014; 9(11): e112149. CrossRef
Environmental Factors Related to Enterobiasis in a Southeast Region of Korea
Dong-Hee Kim, Min Kyoung Cho, Mi Kyung Park, Shin Ae Kang, Bo Young Kim, Sang Kyun Park, Hak Sun Yu
The Korean Journal of Parasitology.2013; 51(1): 139. CrossRef
Enterobius vermicularis and Risk Factors in Healthy Norwegian Children
Håkon Bøås, German Tapia, John A. Sødahl, Trond Rasmussen, Kjersti S. Rønningen
Pediatric Infectious Disease Journal.2012; 31(9): 927. CrossRef
Prevalence of Enterobius vermicularis among Preschool Children in Muan-gun, Jeollanam-do, Korea
Sung-Hee Hong, Young-Il Jeong, Jin-Hee Lee, Shin-Hyeong Cho, Won-Ja Lee, Sang-Eun Lee
The Korean Journal of Parasitology.2012; 50(3): 259. CrossRef
Impact of health education on the prevalence of enterobiasis in Korean preschool students
In-Soon Kang, Dong-Hee Kim, Hye-Gyung An, Hyun-Mi Son, Min Kyoung Cho, Mi-Kyung Park, Shin Ae Kang, Bo Young Kim, Hak Sun Yu
Acta Tropica.2012; 122(1): 59. CrossRef
Prevalence ofEnterobius vermicularisamong Preschool Children in Gimhae-si, Gyeongsangnam-do, Korea
Sang-Eun Lee, Jin-Hee Lee, Jung-Won Ju, Won-Ja Lee, Shin-Hyeong Cho
The Korean Journal of Parasitology.2011; 49(2): 183. CrossRef
Comparison of Egg Positive Rates ofEnterobius vermicularisamong Preschool Children in Three Korean Localities
Sung-Hee Hong, Sang-Eun Lee, Young-Il Jeong, Won-Ja Lee, Shin-Hyeong Cho
The Korean Journal of Parasitology.2011; 49(4): 441. CrossRef

10,316 View
115 Download
Crossref

Endosymbionts of Acanthamoeba Isolated from Domestic Tap Water in Korea: Seon Hee Choi, Min Kyoung Cho, Soon Cheol Ahn, Ji Eun Lee, Jong Soo Lee, Dong-Hee Kim, Ying-Hua Xuan, Yeon Chul Hong, Hyun Hee Kong, Dong Il Chung, Hak Sun Yu; Korean J Parasitol 2009;47(4):337-344.
Published online December 1, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.4.337

Abstract
PDF

In a previous study, we reported our discovery of Acanthamoeba contamination in domestic tap water; in that study, we determined that some Acanthamoeba strains harbor endosymbiotic bacteria, via our molecular characterization by mitochondrial DNA restriction fragment length polymorphism (Mt DNA RFLP). Five (29.4%) among 17 Acanthamoeba isolates contained endosymbionts in their cytoplasm, as demonstrated via orcein staining. In order to estimate their pathogenicity, we conducted a genetic characterization of the endosymbionts in Acanthamoeba isolated from domestic tap water via 16S rDNA sequencing. The endosymbionts of Acanthamoeba sp. KA/WP3 and KA/WP4 evidenced the highest level of similarity, at 97% of the recently published 16S rDNA sequence of the bacterium, Candidatus Amoebophilus asiaticus. The endosymbionts of Acanthamoeba sp. KA/WP8 and KA/WP12 shared a 97% sequence similarity with each other, and were also highly similar to Candidatus Odyssella thessalonicensis, a member of the α-proteobacteria. The endosymbiont of Acanthamoeba sp. KA/WP9 exhibits a high degree of similarity (85-95%) with genus Methylophilus, which is not yet known to harbor any endosymbionts. This is the first report, to the best of our knowledge, to show that Methylophilus spp. can live in the cytoplasm of Acanthamoeba.

Citations

Citations to this article as recorded by

An Evolutionary-Focused Review of the Holosporales (Alphaproteobacteria): Diversity, Host Interactions, and Taxonomic Re-ranking as Holosporineae Subord. Nov
Michele Castelli, Giulio Petroni
Microbial Ecology.2025;[Epub] CrossRef
Zooming in on the intracellular microbiome composition of bacterivorous Acanthamoeba isolates
Binod Rayamajhee, Mark Willcox, Savitri Sharma, Ronnie Mooney, Constantinos Petsoglou, Paul R Badenoch, Samendra Sherchan, Fiona L Henriquez, Nicole Carnt
ISME Communications.2024;[Epub] CrossRef
Acanthamoeba species isolated from marine water in Malaysia exhibit distinct genotypes and variable physiological properties
Rosnani Hanim Mohd Hussain, Mohamed Kamel Abdul Ghani, Naveed Ahmed Khan, Ruqaiyyah Siddiqui, Tengku Shahrul Anuar
Journal of Water and Health.2022; 20(1): 54. CrossRef
The life cycle-dependent transcriptional profile of the obligate intracellular amoeba symbiontAmoebophilus asiaticus
E Selberherr, T Penz, L König, B Conrady, A Siegl, M Horn, S Schmitz-Esser
FEMS Microbiology Ecology.2022;[Epub] CrossRef
A Systematic Review of Intracellular Microorganisms within Acanthamoeba to Understand Potential Impact for Infection
Binod Rayamajhee, Dinesh Subedi, Hari Kumar Peguda, Mark Duncan Willcox, Fiona L. Henriquez, Nicole Carnt
Pathogens.2021; 10(2): 225. CrossRef
A comparative analysis of drinking water employing metagenomics
Kyle D. Brumfield, Nur A. Hasan, Menu B. Leddy, Joseph A. Cotruvo, Shah M. Rashed, Rita R. Colwell, Anwar Huq, Christopher Staley
PLOS ONE.2020; 15(4): e0231210. CrossRef
Detection of bacterial endosymbionts in freshwater crustaceans: the applicability of non-degenerate primers to amplify the bacterial 16S rRNA gene
Monika Mioduchowska, Michał Jan Czyż, Bartłomiej Gołdyn, Adrianna Kilikowska, Tadeusz Namiotko, Tom Pinceel, Małgorzata Łaciak, Jerzy Sell
PeerJ.2018; 6: e6039. CrossRef
Interactive effects of temperature, organic carbon, and pipe material on microbiota composition and Legionella pneumophila in hot water plumbing systems
Caitlin R. Proctor, Dongjuan Dai, Marc A. Edwards, Amy Pruden
Microbiome.2017;[Epub] CrossRef
Isolation and Genotyping of Acanthamoeba spp. as Neglected Parasites in North of Iran
Azar Shokri, Shahabeddin Sarvi, Ahmad Daryani, Mehdi Sharif
The Korean Journal of Parasitology.2016; 54(4): 447. CrossRef
Identification of Paenibacillus as a Symbiont in Acanthamoeba
Vinicius José Maschio, Gertrudes Corção, Francielle Bücker, Karin Caumo, Marilise Brittes Rott
Current Microbiology.2015; 71(3): 415. CrossRef
Ankyrin‐repeat proteins from sponge symbionts modulate amoebal phagocytosis
Mary T. H. D. Nguyen, Michael Liu, Torsten Thomas
Molecular Ecology.2014; 23(6): 1635. CrossRef
Infectious ophthalmology, a neglected field in clinical microbiology
M. Drancourt
Clinical Microbiology and Infection.2013; 19(3): 209. CrossRef
Isolation and molecular characterization of potentially pathogenic Acanthamoeba genotypes from diverse water resources including household drinking water from Khyber Pakhtunkhwa, Pakistan
Tania Tanveer, Abdul Hameed, Ambreen Gul Muazzam, Suk-Yul Jung, Asma Gul, Abdul Matin
Parasitology Research.2013; 112(8): 2925. CrossRef
Vahlkampfia sp: Structural observations of cultured trophozoites
Arturo González-Robles, Lizbeth Salazar-Villatoro, Mónica González-Lázaro, Maritza Omaña-Molina, Adolfo Martínez-Palomo
Experimental Parasitology.2012; 130(1): 86. CrossRef
Diversity of bacteria and mycobacteria in biofilms of two urban drinking water distribution systems
Ruyin Liu, Zhisheng Yu, Hongxun Zhang, Min Yang, Baoyou Shi, Xinchun Liu
Canadian Journal of Microbiology.2012; 58(3): 261. CrossRef
A bacterial genome in transition - an exceptional enrichment of IS elements but lack of evidence for recent transposition in the symbiont Amoebophilus asiaticus
Stephan Schmitz-Esser, Thomas Penz, Anja Spang, Matthias Horn
BMC Evolutionary Biology.2011;[Epub] CrossRef

11,482 View
123 Download
Crossref

Expressed Sequence Tags of Trichinella spiralis Muscle Stage Larvae: Hae Kyung Park, Seong Won Chang, Se Won Kang, Min Kyoung Cho, Sun Hee Choi, Yeon Chul Hong, Yong Seok Lee, Hae Jin Jeong, Hak Sun Yu; Korean J Parasitol 2008;46(2):59-63.
Published online June 20, 2008; DOI: https://doi.org/10.3347/kjp.2008.46.2.59

Abstract
PDF

In order to obtain greater insight into the relevant genomic expression patterns of Trichinella spiralis, 992 expressed sequence tags (ESTs) were collected from a cDNA library of T. spiralis muscle stage larvae and assembled into 60 clusters and 385 singletons. Of them, 445 (44.7%) ESTs were annotated to their homologous genes, and small fractions were matched to known genes of nematodes. The annotated ESTs were classified into 25 eukaryotic orthologous groups (KOG). Cytochrome C oxidase (34 clones) was found to be most frequent species.

Citations

Citations to this article as recorded by

Characterisation of a high-frequency gene encoding a strongly antigenic cystatin-like protein from Trichinella spiralis at its early invasion stage
Bin Tang, Mingyuan Liu, Libo Wang, Shenye Yu, Haining Shi, Pascal Boireau, Vasile Cozma, Xiuping Wu, Xiaolei Liu
Parasites & Vectors.2015;[Epub] CrossRef
Molecular characterization of 45kDa aspartic protease of Trichinella spiralis
Jong Nam Park, Sang Kyun Park, Min Kyoung Cho, Mi-Kyung Park, Shin Ae Kang, Dong-Hee Kim, Hak Sun Yu
Veterinary Parasitology.2012; 190(3-4): 510. CrossRef
Protease-Activated Receptor 2 Is Involved in Th2 Responses againstTrichinella spiralisInfection
Mi Kyung Park, Min Kyoung Cho, Shin Ae Kang, Hye-Kyung Park, Yun Seong Kim, Ki Uk Kim, Soon Cheol Ahn, Dong-Hee Kim, Hak Sun Yu
The Korean Journal of Parasitology.2011; 49(3): 235. CrossRef
Identification of host immune regulation candidate genes of Toxascaris leonina by expression sequenced tags (ESTs) analysis
Min Kyoung Cho, Keun Hee Lee, Sun Joo Lee, Se Won Kang, Mee Sun Ock, Yeon Chul Hong, Yong Seok Lee, Hak Sun Yu
Veterinary Parasitology.2009; 164(2-4): 242. CrossRef