Parasites, Hosts and Diseases

"Myeong-Heon Shin"

Original Article

Excretory-secretory product of newly excysted metacercariae of Paragonimus westermani directly induces eosinophil apoptosis: Myeong-Heon Shin; Korean J Parasitol 2000;38(1):17-23.
Published online March 31, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.1.17

Abstract
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Eosinophils are important effector cells in host defense against parasites. Excretory-secretory product (ESP) produced by helminthic worms plays important roles in the uptake of nutrients, migration in the host tissue, and in immune modulation. However, little is known about the ability of the ESP to directly trigger eosinophil apoptosis. This study investigated whether the ESP of newly excysted metacercariae of Paragonimus westermani could induce apoptosis in human eosinophils. Apoptosis was assayed by staining the cells with FITC-annexin V, and the cells were analyzed by flow cytometry. It was found that the ESP of newly excysted metacercariae of P. westermani induced a direct time- and concentration-dependent increase in the rate of constitutive apoptosis in mature human eosinophils. Eosinophil apoptosis was first apparent 3 hr after treatment with the ESP and continued to increase after 6 hr of incubation with respect to the cells cultured in the absence of the ESP. While only 2.8% of the eosinophils incubated in the medium for 3 hr were apoptotic, 7.6%, 10.9% and 22.6% of the eosinophils treated with 10, 30 and 100 ?g/ml ESP were apoptotic, respectively. This result suggests that the ESP of newly excysted metacercariae of P. westermani directly induce eosinophil apoptosis, which may be important for the survival of the parasites and the reduction of eosinophilic inflammation in vivo.

Citations

Citations to this article as recorded by

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Fasciola hepatica induces eosinophil apoptosis in the migratory and biliary stages of infection in sheep
A. Escamilla, M.J. Bautista, R. Zafra, I.L. Pacheco, M.T. Ruiz, S. Martínez-Cruz, A. Méndez, A. Martínez-Moreno, V. Molina-Hernández, J. Pérez
Veterinary Parasitology.2016; 216: 84. CrossRef
Fasciola hepatica products induce apoptosis of peritoneal macrophages
Lorena Guasconi, Marianela C. Serradell, Diana T. Masih
Veterinary Immunology and Immunopathology.2012; 148(3-4): 359. CrossRef
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Lynne R. Prince, Kirstie J. Graham, John Connolly, Sadia Anwar, Robert Ridley, Ian Sabroe, Simon J. Foster, Moira K. B. Whyte, Malcolm James Horsburgh
PLoS ONE.2012; 7(2): e31506. CrossRef
A 27 kDa Cysteine Protease Secreted by Newly Excysted Paragonimus westermani Metacercariae Induces Superoxide Anion Production and Degranulation of Human Eosinophils
Young-Bae Chung, Hirohito Kita, Myeong Heon Shin
The Korean Journal of Parasitology.2008; 46(2): 95. CrossRef
Excretory-secretory products from Fasciola hepatica induce eosinophil apoptosis by a caspase-dependent mechanism
Marianela C. Serradell, Lorena Guasconi, Laura Cervi, Laura S. Chiapello, Diana T. Masih
Veterinary Immunology and Immunopathology.2007; 117(3-4): 197. CrossRef
Excretory-Secretory Products Produced by Paragonimus westermani Differentially Regulate the Nitric Oxide Production and Viability of Microglial Cells
Youngnam Jin, Jae-Chul Lee, In Young Choi, Eun A. Kim, Myeong Heon Shin, Won-Ki Kim
International Archives of Allergy and Immunology.2006; 139(1): 16. CrossRef
Cysteine Protease Secreted byParagonimus westermaniAttenuates Effector Functions of Human Eosinophils Stimulated with Immunoglobulin G
W. A. Petri, Myeong Heon Shin, Hirohito Kita, Hae Young Park, Ju Young Seoh
Infection and Immunity.2001; 69(3): 1599. CrossRef
Proteolytic activity of cysteine protease in excretory–secretory product of Paragonimus westermani newly excysted metacercariae pivotally regulates IL‐8 production of human eosinophils
Myeong Heon Shin, Soo Young Lee
Parasite Immunology.2000; 22(10): 529. CrossRef

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Brief Communications

Infection status of Paragonimus westermani metacercariae in crayfish (Cambaroides similis) collected from Bogildo (Islet), Wando-gun, Chollanam-do, Korea: Myeong-Heon Shin, Duk-Young Min; Korean J Parasitol 1999;37(1):55-57.
Published online March 31, 1999; DOI: https://doi.org/10.3347/kjp.1999.37.1.55

Abstract
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During the period from October 1996 to November 1998, the infection status of Paragonimus westermani metacercariae in freshwater crayfish (Cambaroides similis) collected from Bogildo (islet), Wando-gun, Chollanam-do, which is known for an endemic area of P. westermani in Korea, were examined. The average infection rate of Paragonimus metacercariae in crayfish was 88.6%, and mean number of metacercariae per infected crayfish was 30.2. This metacercarial density was the highest in the group of weight in 7.1-9.0 g. These results suggest that the natural life cycle of P. westermani is still well-preserved in Bogildo.

Citations

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Recent Incidence of Paragonimus westermani Metacercariae in Freshwater Crayfish, Cambaroides similis, from Two Enzootic Sites in Jeollanam-do, Korea
Jin-Ho Song, Fuhong Dai, Xuelian Bai, Tae-Im Kim, Hyun-Jong Yang, Tong-Soo Kim, Shin-Hyung Cho, Sung-Jong Hong
The Korean Journal of Parasitology.2017; 55(3): 347. CrossRef
Northeast India Helminth Parasite Information Database (NEIHPID): Knowledge Base for Helminth Parasites
Devendra Kumar Biswal, Manish Debnath, Graciously Kharumnuid, Welfrank Thongnibah, Veena Tandon, Govindhaswamy Umapathy
PLOS ONE.2016; 11(6): e0157459. CrossRef
Diseases of crayfish: A review
Matt Longshaw
Journal of Invertebrate Pathology.2011; 106(1): 54. CrossRef
Infection Status of Freshwater Crabs and Crayfish with Metacercariae of Paragonimus westermani in Korea
Eun-Min Kim, Jae-Lip Kim, Sung-Il Choi, Soon-Hyung Lee, Sung-Tae Hong
The Korean Journal of Parasitology.2009; 47(4): 425. CrossRef
North American Paragonimiasis (Caused byParagonimus kellicotti) in the Context of Global Paragonimiasis
Gary W. Procop
Clinical Microbiology Reviews.2009; 22(3): 415. CrossRef
A 27 kDa Cysteine Protease Secreted by Newly Excysted Paragonimus westermani Metacercariae Induces Superoxide Anion Production and Degranulation of Human Eosinophils
Young-Bae Chung, Hirohito Kita, Myeong Heon Shin
The Korean Journal of Parasitology.2008; 46(2): 95. CrossRef
Cysteine Protease Secreted byParagonimus westermaniAttenuates Effector Functions of Human Eosinophils Stimulated with Immunoglobulin G
W. A. Petri, Myeong Heon Shin, Hirohito Kita, Hae Young Park, Ju Young Seoh
Infection and Immunity.2001; 69(3): 1599. CrossRef

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Genetic variance of Trichomonas vaginalis isolates by Southern hybridization: Jae-Sook Ryu, Duk-Young Min, Myeong-Heon Shin, Youl-Hee Cho; Korean J Parasitol 1998;36(3):207-211.
Published online September 20, 1998; DOI: https://doi.org/10.3347/kjp.1998.36.3.207

Abstract
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In the present study, genomic DNAs were purified from Korean isolates (KT8, KT6, KT-Kim and KT-Lee) and foreign strains (CDC85, IR78 and NYH 286) of Trichomonas vaginalis, and hybridized with a probe based on the repetitive sequence cloned from T. vaginalis to observe the genetic differences. By Southern hybridization, all isolates of T. vaginalis except the NYH286 strain had 11 bands. Therefore all isolates examined were distinguishable into 3 groups according to their banding patterns; i) KT8, KT6 and KT-Kim isolates had 11 identical bands such as 1 kb, 1.2 kb, 1.6 kb, 1.9 kb, 2.3 kb, 2.7 kb, 3.2 kb, 3.4 kb, 3.8 kb, 4.9 kb and 6.0 kb. ii) The metronidazole-resistant IR78 strain had the same bands as KT-Lee isolate at bands of 1 kb, 1.2 kb, 1.6 kb, 1.8 kb, 2.1 kb, 2.5 kb, 2.7 kb, 2.9 kb, 3.4 kb, 5.0 kb and 6.0 kb. Bands of CDC85, metronidazole-resistant strain, were similar to those of IR78 and KT-Lee, except that 3.2 kb replaced 2.9 kb. iii) NYH286 particularly had 12 bands and band patterns were similar to IR78 with a few exceptions as follows: i) 6.2 kb in place of 6.0 kb, ii) 2.0 kb and 2.2 kb instead of 2.1 kb. Through the results obtained, genetic variance of T. vaginalis isolates was demonstrated by Southern hybridization.

Citations

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