Toxoplasma gondii penetrates all kinds of nucleated eukaryotic cells but modulates host cells differently for its intracellular survival. In a previous study, we found out that serine protease inhibitors B3 and B4 (SERPIN B3/B4 because of their very high homology) were significantly induced in THP-1-derived macrophages infected with T. gondii through activation of STAT6. In this study, to evaluate the effects of the induced SERPIN B3/B4 on the apoptosis of T. gondii-infected THP-1 cells, we designed and tested various small interfering (si-) RNAs of SERPIN B3 or B4 in staurosporine-induced apoptosis of THP-1 cells. Anti-apoptotic characteristics of THP-1 cells after infection with T. gondii disappeared when SERPIN B3/B4 were knock-downed with gene specific si-RNAs transfected into THP-1 cells as detected by the cleaved caspase 3, poly-ADP ribose polymerase and DNA fragmentation. This anti-apoptotic effect was confirmed in SERPIN B3/B4 overexpressed HeLa cells. We also investigated whether inhibition of STAT6 affects the function of SERPIN B3/B4, and vice versa. Inhibition of SERPIN B3/B4 did not influence STAT6 expression but SERPIN B3/B4 expression was inhibited by STAT6 si-RNA transfection, which confirmed that SERPIN B3/B4 was induced under the control of STAT6 activation. These results suggest that T. gondii induces SERPIN B3/B4 expression via STAT6 activation to inhibit the apoptosis of infected THP-1 cells for longer survival of the intracellular parasites themselves.
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Secretome Analysis of Host Cells Infected with Toxoplasma gondii after Treatment of Human Epidermal Growth Factor Receptor 2/4 Inhibitors Hye-Jung Kim, Hye-Jin Ahn, Hyeweon Kang, Jaehui Park, Seul gi Oh, Saehae Choi, Won-Kyu Lee, Ho-Woo Nam The Korean Journal of Parasitology.2020; 58(3): 249. CrossRef
Toxoplasma gondiiModulates the Host Cell Responses: An Overview of Apoptosis Pathways Nour Mammari, Mohamad Adnan Halabi, Souha Yaacoub, Hilda Chlala, Marie-Laure Dardé, Bertrand Courtioux BioMed Research International.2019; 2019: 1. CrossRef
Mechanisms of Human Innate Immune Evasion by Toxoplasma gondii Tatiane S. Lima, Melissa B. Lodoen Frontiers in Cellular and Infection Microbiology.2019;[Epub] CrossRef
Strategies Developed by Toxoplasma gondii to Survive in the Host Wanbo Zhu, Jingyang Li, Faustina Pappoe, Jilong Shen, Li Yu Frontiers in Microbiology.2019;[Epub] CrossRef
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