Parasites, Hosts and Diseases

"Sang-Han Lee"

Brief Communications

House Dust Mite Allergens in Domestic Homes in Cheonan, Korea: Hae-Seon Nam, Robert Siebers, Sun-Hwa Lee, Joon-Soo Park, Yong-Bae Kim, Young-Jin Choi, Sang-Han Lee, Julian Crane; Korean J Parasitol 2008;46(3):187-189.
Published online September 20, 2008; DOI: https://doi.org/10.3347/kjp.2008.46.3.187

Abstract
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House dust mites produce inhalant allergens of importance to allergic patients. We measured the major group 1 allergens, Der p 1 and Der f 1, from the house dust mites Dermatophagoides pteronyssinus and Dermatophagoides farina, respectively in 100 randomly selected domestic homes from Cheonan, Korea. Dust samples were collected by vacuuming from the living room floor and 1 mattress in each home. Der p 1 and Der f 1 were measured by double monoclonal ELISA. Der p 1 levels were very low, with geometric mean levels for floors and mattresses being 0.11 ?g/g (range: 0.01-4.05) and 0.14 ?g/g (range: 0.01-30.0), respectively. Corresponding levels of Der f 1 were higher, 7.46 ?g/g (range: 0.01-262.9) and 10.2 ?g/g (range: 0.01-230.9) for floors and mattresses, respectively. D. farinae appears to be the dominant house dust mite in Cheonan.

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Validity of MAST-CLA for diagnosis of arthropod allergy using receiver operating characteristic (ROC) analysis: Joon-Soo Park, Hae-Seon Nam, Yong-Bae Kim, Young-Jin Choi, Sang-Han Lee, Sung-Ho Kim; Korean J Parasitol 2007;45(3):239-243.
Published online September 20, 2007; DOI: https://doi.org/10.3347/kjp.2007.45.3.239

Abstract
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Many allergists are currently focusing on the development of new diagnostic tools, and are attempting to improve both the sensitivity and specificity. A multiple allergen simultaneous test-chemiluminescent assay (MAST-CLA) is one of the most popular diagnostic tools used in the Republic of Korea. However, there remains controversy among allergists with regard to the cut-off point for a positive result. The present study was conducted in order to determine the validity of MAST-CLA as compared with that of the skin prick test, with particular emphasis on arthropod allergens, on the basis of percentage agreement rates and κ-values, and also to suggest the optimal positive cut-off points using receiver operating characteristic (ROC) curves. The study was conducted with 97 subjects (54 men, 43 women). Optimal individual cut-off points were calculated as follows; class II for Dermatophagoides farinae, class I for Dermatophagoides pteronyssinus, and trace for a cockroach mix. These findings suggest that attempting to apply optimal individual cut-off points will be a good way of improving diagnostic tests, particularly MAST-CLA.

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Comparison Study between MAST CLA and OPTIGEN
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