Parasites, Hosts and Diseases

"Sudchit Chungpivat"

Brief Communication

Successful Treatment of Brugia pahangi in Naturally Infected Cats with Ivermectin: Piyanan Taweethavonsawat, Sudchit Chungpivat; Korean J Parasitol 2013;51(6):759-761.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.759

Abstract
PDF

Lymphatic filariasis is a common parasitic disease of cats in tropical regions including Thailand. The
objective
of this study was to determine the efficacy of ivermectin against microfilariae of Brugia pahangi in naturally infected cats. Eight cats naturally infected with B. pahangi were divided into control (untreated) and treated groups. Cats in the latter group were given ivermectin injection at 400 ?g/kg weekly for 2 months. Microfilariae were counted every week until 48 weeks. Microfilaremia was significantly decreased in the treated group 4 weeks after starting the treatment and become zero at week 9 and afterwards. On the other hand, cats in the control group had high microfilaremia throughout the study. It was successful to treat and control B. pahangi infection in naturally infected cats using ivermectin.

Citations

Citations to this article as recorded by

Filariasis of the breast caused by Brugia pahangi: A concomitant finding with invasive ductal carcinoma
Jerapas Thongpiya, Doonyapat Sa-nguanraksa, Norasate Samarnthai, Patsharaporn T. Sarasombath
Parasitology International.2021; 80: 102203. CrossRef
Comparison of efficacy of ivermectin and diethylcarbamazine against naturally infected Brugia malayi microfilaria in dogs
Poojary Vineeta Sadarama, Deepa Chirayath, Usha Narayana Pillai, Bindu Lakshmanan
Journal of Parasitic Diseases.2019; 43(4): 554. CrossRef
Therapeutic trial of doxycyclin plus ivermectin for the treatment of Brugia malayi naturally infected cats
Ladawan Khowawisetsut, Patsharaporn T. Sarasombath, Suwich Thammapalo, Sumart Loymek, Therayot Korbarsa, Hathai Nochote, Achinya Phuakrod, Wej Choochote, Sirichit Wongkamchai
Veterinary Parasitology.2017; 245: 42. CrossRef

7,660 View
88 Download
Crossref

Original Article

Rapid Detection and Identification of Wuchereria bancrofti, Brugia malayi, B. pahangi, and Dirofilaria immitis in Mosquito Vectors and Blood Samples by High Resolution Melting Real-Time P: Tongjit Thanchomnang, Pewpan M. Intapan, Chairat Tantrawatpan, Viraphong Lulitanond, Sudchit Chungpivat, Piyanan Taweethavonsawat, Worasak Kaewkong, Oranuch Sanpool, Penchom Janwan, Wej Choochote, Wanchai Maleewong; Korean J Parasitol 2013;51(6):645-650.
Published online December 31, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.6.645

Abstract
PDF

A simple, rapid, and high-throughput method for detection and identification of Wuchereria bancrofti, Brugia malayi, Brugia pahangi, and Dirofilaria immitis in mosquito vectors and blood samples was developed using a real-time PCR combined with high-resolution melting (HRM) analysis. Amplicons of the 4 filarial species were generated from 5S rRNA and spliced leader sequences by the real-time PCR and their melting temperatures were determined by the HRM method. Melting of amplicons from W. bancrofti, B. malayi, D. immitis, and B. pahangi peaked at 81.5±0.2℃, 79.0±0.3℃, 76.8±0.1℃, and 79.9±0.1℃, respectively. This assay is relatively cheap since it does not require synthesis of hybridization probes. Its sensitivity and specificity were 100%. It is a rapid and technically simple approach, and an important tool for population surveys as well as molecular xenomonitoring of parasites in vectors.

Citations

Citations to this article as recorded by

Beyond Tradition: Exploring Cutting-Edge Approaches for Accurate Diagnosis of Human Filariasis
Damian Pietrzak, Julia Weronika Łuczak, Marcin Wiśniewski
Pathogens.2024; 13(6): 447. CrossRef
Detecting Dirofilaria immitis: Current Practices and Novel Diagnostic Methods
Damian Pietrzak, Julia Weronika Łuczak, Marcin Wiśniewski
Pathogens.2024; 13(11): 950. CrossRef
IDENTIFICATION OF MICROFILARIAE USING CONVENTIONAL POLYMERASE CHAIN REACTION AND QPCR-HRM
Bagus Muhammad Ihsan, Widyana Lakshmi Puspita, Linda Triana, Wahdaniah, Khayan, Cecep Dani Sucipto
Journal of Vocational Health Studies.2024; 8(1): 42. CrossRef
Mosquitoes, Lymphatic Filariasis, and Public Health: A Systematic Review of Anopheles and Aedes Surveillance Strategies
Arumugam Bhuvaneswari, Ananganallur Nagarajan Shriram, Kishan Hari K. Raju, Ashwani Kumar
Pathogens.2023; 12(12): 1406. CrossRef
Microfilaria Positification Test Using Real-Time PCR Technique with HRM (High-Resolution Melting)
Bagus Muhammad Ihsan, Afiat Berbudi, Ridad Agoes, Muhammad Ifham Hanif
Acta Parasitologica.2022; 67(1): 496. CrossRef
Collection and DNA Detection of Dirofilaria immitis (Rhabditida Onchocercidae), Using a Novel Primer Set, in Wild-Caught Mosquitoes From Gainesville, FL
Chris Holderman, Nicole O Abruzzo, Noor A Abdelsamad, Phillip E Kaufman, Peter M DiGennaro, Stephen Rich
Journal of Medical Entomology.2021; 58(3): 1429. CrossRef
The use of molecular xenomonitoring for surveillance of mosquito-borne diseases
Mary M. Cameron, Anita Ramesh
Philosophical Transactions of the Royal Society B: Biological Sciences.2021; 376(1818): 20190816. CrossRef
Canine and feline vector-borne diseases of zoonotic concern in Southeast Asia
Viet-Linh Nguyen, Filipe Dantas-Torres, Domenico Otranto
Current Research in Parasitology & Vector-Borne Diseases.2021; 1: 100001. CrossRef
The Incidence of Dirofilaria immitis in Shelter Dogs and Mosquitoes in Austria
Karin Sonnberger, Hans-Peter Fuehrer, Bernhard Werner Sonnberger, Michael Leschnik
Pathogens.2021; 10(5): 550. CrossRef
Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases
Madeleine J. Rogers, Donald P. McManus, Stephen Muhi, Catherine A. Gordon
Clinical Microbiology Reviews.2021;[Epub] CrossRef
PRIMARY SLX TEST USING REAL-TIME PCR BASED ON HIGH RESOLUTION MELTING (HRM) ON MICROFILARIA EXAMINATION
Bagus Muhammad Ihsan, Cecep Dani Sucipto, Khayan Khayan
Journal of Vocational Health Studies.2021; 5(1): 26. CrossRef
Molecular detection of Trichostrongylus species through PCR followed by high resolution melt analysis of ITS-2 rDNA sequences
Mohsen Arbabi, Hossein Hooshyar, Majid Lotfinia, Mohamad Ali Bakhshi
Molecular and Biochemical Parasitology.2020; 236: 111260. CrossRef
Prevalence and risk factors associated with Dirofilaria immitis infection in dogs and cats in Songkhla and Satun provinces, Thailand
Ketsarin Kamyingkird, Witchuta Junsiri, Witsanuwat Chimnoi, Chanya Kengradomkij, Sinsamut Saengow, Keplee Sangchuto, Wicha Kajeerum, Decha Pangjai, Burin Nimsuphan, Tawin Inpankeaw, Sathaporn Jittapalapong
Agriculture and Natural Resources.2017; 51(4): 299. CrossRef
Is molecular xenomonitoring of mosquitoes for Dirofilaria repens suitable for dirofilariosis surveillance in endemic regions?
Aleksander Masny, Rusłan Sałamatin, Wioletta Rozej-Bielicka, Elzbieta Golab
Parasitology Research.2016; 115(2): 511. CrossRef
Pyrosequencing Using SL and 5S rRNA as Molecular Markers for Identifying Zoonotic Filarial Nematodes in Blood Samples and Mosquitoes
Oranuch Sanpool, Chairat Tantrawatpan, Tongjit Thanchomnang, Penchom Janwan, Pewpan M. Intapan, Rutchanee Rodpai, Viraphong Lulitanond, Piyanan Taweethavonsawat, Wanchai Maleewong
Vector-Borne and Zoonotic Diseases.2016; 16(5): 326. CrossRef
Detection and quantification of Wuchereria bancrofti and Brugia malayi DNA in blood samples and mosquitoes using duplex droplet digital polymerase chain reaction
Jurairat Jongthawin, Pewpan M. Intapan, Viraphong Lulitanond, Oranuch Sanpool, Tongjit Thanchomnang, Lakkhana Sadaow, Wanchai Maleewong
Parasitology Research.2016; 115(8): 2967. CrossRef
Trypanosome infection rates in tsetse flies in the “silent” sleeping sickness focus of Bafia in the Centre Region in Cameroon
Gustave Simo, Pierre Fongho, Oumarou Farikou, Prosper Innocent Ndjeuto Ndjeuto-Tchouli, Judith Tchouomene-Labou, Flobert Njiokou, Tazoacha Asonganyi
Parasites & Vectors.2015;[Epub] CrossRef