Yeong Hoon Kim, Tae-Yun Kim, Ji-Seon Park, Jin Suk Park, Jihoo Lee, Joungdae Moon, Chom-Kyu Chong, Ivan Neves Junior, Fernando Raphael Ferry, Hye-Jin Ahn, Lokraj Bhatt, Ho-Woo Nam
Korean J Parasitol 2019;57(3):283-290. Published online June 30, 2019
A rapid diagnostic test (RDT) kit was developed to detect non-structural protein 1 (NS1) of yellow fever virus (YFV) using monoclonal antibody. NS1 protein was purified from the cultured YFV and used to immunize mice. Monoclonal antibody to NS1 was selected and conjugated with colloidal gold to produce the YFV NS1 RDT kit. The YFV RDTs were evaluated for sensitivity and specificity using positive and negative samples of monkeys from Brazil and negative human blood samples from Korea. Among monoclonal antibodies, clones 3A11 and 3B7 proved most sensitive, and used for YFV RDT kit. Diagnostic accuracy of YFV RDT was fairly high; Sensitivity was 0.0% and specificity was 100% against Dengue viruses type 2 and 3, Zika, Chikungunya and Mayaro viruses. This YFV RDT kit could be employed as a test of choice for point-of-care diagnosis and large scale surveys of YFV infection under clinical or field conditions in endemic areas and on the globe.
Citations
Citations to this article as recorded by
Synthesis of Truncated DNA Aptamer and Its Application to an Electrochemical Biosensor Consisting of an Aptamer and a MXene Heterolayer for Yellow Fever Virus Nayeon Kwon, Siyun Lee, Moonbong Jang, Jin-Ho Lee, Chulhwan Park, Taek Lee BioChip Journal.2024; 18(1): 93. CrossRef
Challenges in Direct Detection of Flaviviruses: A Review Bruna de Paula Dias, Camila Cavadas Barbosa, Cyntia Silva Ferreira, Samara Mayra Soares Alves dos Santos, Orlando Alfredo Pineda Arrieta, Wellington Carvalho Malta, Maria Laura Maximiano Dias Gomes, Mariela Alves e Silva, Júlia de Matos Fonseca, Lysandro Pathogens.2023; 12(5): 643. CrossRef
A Chikungunya Virus Multiepitope Recombinant Protein Expressed from the Binary System Insect Cell/Recombinant Baculovirus Is Useful for Laboratorial Diagnosis of Chikungunya Leonardo Assis da Silva, Monique da Rocha Queiroz Lima, Brenda Rabello de Camargo, Dyeferson Kened da Silva Coelho Guimarães, Anabele Azevedo Lima Barbastefano, Raquel Curtinhas de Lima, Paulo Vieira Damasco, Rivaldo Venâncio da Cunha, Luiz José de Souza, Microorganisms.2022; 10(7): 1451. CrossRef
In this study, we found that Haplorchis taichui, a heterophyid intestinal fluke, is highly prevalent, with heavy worm loads, among riparian people in Saravane and Champasak province, Lao PDR. Fecal specimens were collected from 1,460 people (717 men and 743 women) in 12 riparian (Mekong river) districts and were examined by the Kato-Katz fecal smear technique. The overall helminth egg positive rate was 78.8% and 66.4% in Saravane and Champasak province, respectively. The positive rate for small trematode eggs (STE), which included H. taichui and other heterophyids, Opisthorchis viverrini, and lecithodendriids, was 69.9% and 46.3% in Saravane and Champasak province, respectively. To obtain adult flukes, 30 STE-positive people were treated with 40 mg/kg praziquantel and then purged. Whole diarrheic stools were collected 4-5 times for each person and searched for fluke specimens using a stereomicroscope. Mixed infections with various species of trematodes (H. taichui, Haplorchis pumilio, O. viverrini, Prosthodendrium molenkampi, Centrocestus formosanus, and Echinochasmus japonicus) and a species of cestode (Taenia saginata) were found. However, the worm load was exceptionally high for H. taichui compared with other trematode species, with an average of 21,565 and 12,079 specimens per infected person in Saravane and Champasak province, respectively, followed by H. pumilio (41.9 and 22.5, respectively) and O. viverrini (9.4 and 1.5, respectively). These results show that diverse species of intestinal and liver flukes are prevalent among riparian people in Saravane and Champasak province, Lao PDR, with H. taichui being the exceptionally dominant species.
Citations
Citations to this article as recorded by
An imported case of Haplorchis taichui infection diagnosed with molecular genomics in France Victor Luzarraga, Maxime Moniot, Patricia Combes, Mathilde Legay, Philippe Poirier, Céline Nourrisson Travel Medicine and Infectious Disease.2025; 67: 102888. CrossRef
High endemicity of Opisthorchis viverrini infection among people in northern Cambodia confirmed by adult worm expulsion Bong-Kwang Jung, Sooji Hong, Taehee Chang, Jaeeun Cho, Seungwan Ryoo, Keon Hoon Lee, Jeonggyu Lee, Woon-Mok Sohn, Sung-Jong Hong, Virak Khieu, Rekol Huy, Jong-Yil Chai Scientific Reports.2023;[Epub] CrossRef
Multiplex PCR development for the simultaneous and rapid detection of two pathogenic flukes, Dactylogyrus spp. and Centrocestus formosanus, in ornamental fishes Phonkawin Jaruboonyakorn, Thanawan Tejangkura, Thapana Chontananarth Aquaculture.2022; 548: 737660. CrossRef
Performance of Mini Parasep® SF stool concentrator kit, Kato-Katz, and formalin-ethyl acetate concentration methods for diagnosis of opisthorchiasis in Northeast Thailand Kulthida Y. Kopolrat, Seri Singthong, Narong Khuntikeo, Watcharin Loilome, Chanika Worasith, Chutima Homwong, Chompunoot Wangboon, Patiwat Yasaka, Chatanun Eamudomkarn, Opal Pitaksakulrat, Krisnakorn Tonkhamhak, Arunee Paeyo, Thomas Crellen, Jiraporn Sith Parasites & Vectors.2022;[Epub] CrossRef
General overview of the current status of human foodborne trematodiasis Jong-Yil Chai, Bong-Kwang Jung Parasitology.2022; 149(10): 1262. CrossRef
Model-based spatial-temporal mapping of opisthorchiasis in endemic countries of Southeast Asia Ting-Ting Zhao, Yi-Jing Feng, Pham Ngoc Doanh, Somphou Sayasone, Virak Khieu, Choosak Nithikathkul, Men-Bao Qian, Yuan-Tao Hao, Ying-Si Lai eLife.2021;[Epub] CrossRef
Foodborne intestinal flukes: A brief review of epidemiology and geographical distribution Jong-Yil Chai, Bong-Kwang Jung Acta Tropica.2020; 201: 105210. CrossRef
Echinostoma aegyptica (Trematoda: Echinostomatidae) Infection in Five Riparian People in Savannakhet Province, Lao PDR Jong-Yil Chai, Bong-Kwang Jung, Taehee Chang, Hyejoo Shin, Woon-Mok Sohn, Keeseon S. Eom, Tai-Soon Yong, Duk-Young Min, Bounlay Phammasack, Bounnaloth Insisiengmay, Han-Jong Rim The Korean Journal of Parasitology.2020; 58(1): 67. CrossRef
Diagnosis of Centrocestus formosanus Infection in Zebrafish (Danio rerio) in Italy: A Window to a New Globalization-Derived Invasive Microorganism Antonino Pace, Ludovico Dipineto, Serena Aceto, Maria Concetta Censullo, Maria Carmen Valoroso, Lorena Varriale, Laura Rinaldi, Lucia Francesca Menna, Alessandro Fioretti, Luca Borrelli Animals.2020; 10(3): 456. CrossRef
Echinochasmus caninus n. comb. (Trematoda: Echinostomatidae) Infection in Eleven Riparian People in Khammouane Province, Lao PDR Jong-Yil Chai, Taehee Chang, Bong-Kwang Jung, Hyejoo Shin, Woon-Mok Sohn, Keeseon S. Eom, Tai-Soon Yong, Duk-Young Min, Bounlay Phammasack, Bounnaloth Insisiengmay, Han-Jong Rim The Korean Journal of Parasitology.2019; 57(4): 451. CrossRef
Echinostoma ilocanum Infection in Two Residents of Savannakhet Province, Lao PDR Jong-Yil Chai, Woon-Mok Sohn, Jaeeun Cho, Keeseon S. Eom, Tai-Soon Yong, Duk-Young Min, Eui-Hyug Hoang, Bounlay Phommasack, Bounnaloth Insisiengmay, Han-Jong Rim The Korean Journal of Parasitology.2018; 56(1): 75. CrossRef
Ultrasonographic investigation of cholangiocarcinoma in Lao PDR Ju Yeong Kim, Tai-Soon Yong, Han-Jong Rim, Jong-Yil Chai, Duk-Young Min, Keeseon S. Eom, Woon-Mok Sohn, Jae Hoon Lim, Dongil Choi, Sithat Insisiengmay, Bounlay Phommasack, Bounnaloth Insisiengmay Acta Tropica.2018; 182: 128. CrossRef
The rapid detection method by polymerase chain reaction for minute intestinal trematodes: Haplorchis taichui in intermediate snail hosts based on 18s ribosomal DNA Thapana Chontananarth, Sothorn Anucherngchai, Thanawan Tejangkura Journal of Parasitic Diseases.2018; 42(3): 423. CrossRef
Fishborne zoonotic heterophyid infections: An update Jong-Yil Chai, Bong-Kwang Jung Food and Waterborne Parasitology.2017; 8-9: 33. CrossRef
Perceived illness drives participation in mass deworming campaigns in Laos Khampheng Phongluxa, Peter van Eeuwijk, Phonepasong Ayé Soukhathammavong, Kongsap Akkhavong, Peter Odermatt Acta Tropica.2015; 141: 281. CrossRef
Integrated parasite management: path to sustainable control of fishborne trematodes in aquaculture Jesper H. Clausen, Henry Madsen, Phan Thi Van, Anders Dalsgaard, K. Darwin Murrell Trends in Parasitology.2015; 31(1): 8. CrossRef
Subtle to severe hepatobiliary morbidity in Opisthorchis viverrini endemic settings in southern Laos Phonepasong Ayé Soukhathammavong, Virasack Rajpho, Khampheng Phongluxa, Youthanavanh Vonghachack, Jan Hattendorf, Bouasy Hongvanthong, Oroth Rasaphon, Banchob Sripa, Kongsap Akkhavong, Christoph Hatz, Peter Odermatt Acta Tropica.2015; 141: 303. CrossRef
Reprint of “An overview of freshwater snails in Asia with main focus on Vietnam” H. Madsen, N.M. Hung Acta Tropica.2015; 141: 372. CrossRef
Patterns of trematode infections of Opisthorchis viverrini (Opisthorchiidae) and Haplorchis taichui (Heterophyidae) in human populations from two villages in Savannakhet Province, Lao PDR M. Sato, T. Pongvongsa, S. Sanguankiat, T. Yoonuan, J. Kobayashi, B. Boupha, F. Nishimoto, K. Moji, M.O. Sato, J. Waikagul Journal of Helminthology.2015; 89(4): 439. CrossRef
Susceptibility, metacercarial burden, and mortality of juvenile silver barb, common carp, mrigal, and tilapia following exposure to Haplorchis taichui Kulthida Kopolrat, Paiboon Sithithaworn, Smarn Tesana, Ross H. Andrews, Trevor N. Petney Parasitology Research.2015; 114(4): 1433. CrossRef
Repeated stool sampling and use of multiple techniques enhance the sensitivity of helminth diagnosis: A cross-sectional survey in southern Lao People's Democratic Republic Somphou Sayasone, Jürg Utzinger, Kongsap Akkhavong, Peter Odermatt Acta Tropica.2015; 141: 315. CrossRef
Heterophyid trematodes recovered from people residing along the Boseong River, South Korea Jong-Yil Chai, Bong-Kwang Jung, Deok-Gyu Kim, Jae-Lip Kim, Hyemi Lim, Eun-Hee Shin, Keon Hoon Lee, Mok Ryeon Kim, Sun Jin Han, Jae Hyun Yeom, Sun Mi Park, Jae-Sun Hwang Acta Tropica.2015; 148: 142. CrossRef
Intestinal Helminths Recovered from Humans in Xieng Khouang Province, Lao PDR with a Particular Note on <i>Haplorchis pumilio</i> Infection Jong-Yil Chai, Woon-Mok Sohn, Bong-Kwang Jung, Tai-Soon Yong, Keeseon S. Eom, Duk-Young Min, Bounnaloth Insisiengmay, Sithat Insisiengmay, Bounlay Phommasack, Han-Jong Rim The Korean Journal of Parasitology.2015; 53(4): 439. CrossRef
Risk Profiling of Hookworm Infection and Intensity in Southern Lao People’s Democratic Republic Using Bayesian Models Armelle Forrer, Penelope Vounatsou, Somphou Sayasone, Youthanavanh Vonghachack, Dalouny Bouakhasith, Jürg Utzinger, Kongsap Akkhavong, Peter Odermatt, Guo-Jing Yang PLOS Neglected Tropical Diseases.2015; 9(3): e0003486. CrossRef
An overview of freshwater snails in Asia with main focus on Vietnam H. Madsen, N.M. Hung Acta Tropica.2014; 140: 105. CrossRef
Development of a PCR assay and pyrosequencing for identification of important human fish-borne trematodes and its potential use for detection in fecal specimens Chairat Tantrawatpan, Pewpan M Intapan, Tongjit Thanchomnang, Oranuch Sanpool, Penchom Janwan, Viraphong Lulitanond, Lakkhana Sadaow, Wanchai Maleewong Parasites & Vectors.2014;[Epub] CrossRef
Prevalence of Helminthic Infections among Inhabitants of Lao PDR Keeseon S. Eom, Tai-Soon Yong, Woon-Mok Sohn, Jong-Yil Chai, Duk-Young Min, Han-Jong Rim, Hyeong-Kyu Jeon, Virasack Banouvong, Bounnaloth Insisiengmay, Bounlay Phommasack The Korean Journal of Parasitology.2014; 52(1): 51. CrossRef
Prevalence of Haplorchis taichui among humans and fish in Luang Prabang Province, Lao PDR Woon-Mok Sohn, Tai-Soon Yong, Keeseon S. Eom, Duk-Young Min, Dongmin Lee, Bong-Kwang Jung, Virasack Banouvong, Bounnaloth Insisiengmay, Bounlay Phommasack, Han-Jong Rim, Jong-Yil Chai Acta Tropica.2014; 136: 74. CrossRef
Epidemiology of fishborne trematodiasis in Kazakhstan A. Sultanov, A. Abdybekova, A. Abdibaeva, Z. Shapiyeva, T. Yeshmuratov, P.R. Torgerson Acta Tropica.2014; 138: 60. CrossRef
High Prevalence of Haplorchiasis in Nan and Lampang Provinces, Thailand, Proven by Adult Worm Recovery from Suspected Opisthorchiasis Cases Adulsak Wijit, Nimit Morakote, Jaewwaew Klinchid The Korean Journal of Parasitology.2013; 51(6): 767. CrossRef
Helminth infection in southern Laos: high prevalence and low awareness Khampheng Phongluxa, Vilavanh Xayaseng, Youthanavanh Vonghachack, Kongsap Akkhavong, Peter van Eeuwijk, Peter Odermatt Parasites & Vectors.2013;[Epub] CrossRef
Toxoplasma gondii tachyzoites were isolated from an ocular patient in the Republic of Korea and maintained in the laboratory (designated KI-1). In the present study, its genotype was determined by analyzing dense granule antigen 6 (GRA6) gene and surface antigen 2 (SAG2) gene as typing markers. Digestion of the amplification products of GRA6 and of the 5' and 3' ends of SAG2, respectively, with Mse I, Sau3A I, and Hha I, revealed that KI-1 is included in the genotype I, which includes the worldwide virulent RH strain. In addition, when the whole sequences of the coding regions of SAG1, rhoptry antigen 1 (ROP1), and GRA8 genes of KI-1 were compared with those of RH, minor nucleotide polymorphisms and amino acid substitutions were identified. These results show that KI-1 is a new geographical strain of T. gondii that can be included in the genotype I.
Citations
Citations to this article as recorded by
Geographical distribution of Toxoplasma gondii genotypes in Asia: A link with neighboring continents P. Chaichan, A. Mercier, L. Galal, A. Mahittikorn, F. Ariey, S. Morand, F. Boumédiène, R. Udonsom, A. Hamidovic, J.B. Murat, Y. Sukthana, M.L. Dardé Infection, Genetics and Evolution.2017; 53: 227. CrossRef
<i>Toxoplasma gondii</i> B1 Gene Detection in Feces of Stray Cats around Seoul, Korea and Genotype Analysis of Two Laboratory-Passaged Isolates Bong-Kwang Jung, Sang-Eun Lee, Hyemi Lim, Jaeeun Cho, Deok-Gyu Kim, Hyemi Song, Min-Jae Kim, Eun-Hee Shin, Jong-Yil Chai The Korean Journal of Parasitology.2015; 53(3): 259. CrossRef
Genetic characterization of Toxoplasma gondii isolates from chickens in India by GRA6 gene sequence analysis Shantaveer Biradar, Buddhi Saravanan, Anup Tewari, Chirukandoth Sreekumar, Muthu Sankar, Naduvanahalli Sudhakar Acta Parasitologica.2014;[Epub] CrossRef
Selective and strain-specific NFAT4 activation by the Toxoplasma gondii polymorphic dense granule protein GRA6 Ji Su Ma, Miwa Sasai, Jun Ohshima, Youngae Lee, Hironori Bando, Kiyoshi Takeda, Masahiro Yamamoto Journal of Experimental Medicine.2014; 211(10): 2013. CrossRef
Immune Responses of Mice Intraduodenally Infected withToxoplasma gondiiKI-1 Tachyzoites Eun-Hee Shin, Yeoun Sook Chun, Won-Hee Kim, Jae-Lip Kim, Kyoung-Ho Pyo, Jong-Yil Chai The Korean Journal of Parasitology.2011; 49(2): 115. CrossRef
Proteomic Analysis ofToxoplasma gondiiKI-1 Tachyzoites Si-Hwan Choi, Tae Yun Kim, Sung Goo Park, Guang-Ho Cha, Dae-Whan Shin, Jong-Yil Chai, Young-Ha Lee The Korean Journal of Parasitology.2010; 48(3): 195. CrossRef
Suppression of CD4+T-Cells in the Spleen of Mice Infected withToxoplasma gondiiKI-1 Tachyzoites Won-Hee Kim, Eun-Hee Shin, Jae-Lip Kim, Seung-Young Yu, Bong-Kwang Jung, Jong-Yil Chai The Korean Journal of Parasitology.2010; 48(4): 325. CrossRef
Selection of Polymorphic Peptides from
GRA6
and
GRA7
Sequences of
Toxoplasma gondii
Strains To Be Used in Serotyping
Susana Sousa, Daniel Ajzenberg, Maggy Marle, Dominique Aubert, Isabelle Villena, José Correia da Costa, Marie-Laure Dardé Clinical and Vaccine Immunology.2009; 16(8): 1158. CrossRef
Genotyping of a Korean isolate of Toxoplasma gondii by multilocus PCR-RFLP and microsatellite analysis Juan-Hua Quan, Tae Yun Kim, In-Uk Choi, Young-Ha Lee The Korean Journal of Parasitology.2008; 46(2): 105. CrossRef
Evaluation of the Korean Isolate-1 Tachyzoite Antigen for Serodiagnosis of Toxoplasmosis Eun-Hee Shin, Dong-Hee Kim, Aifen Lin, Jo-Woon-Yi Lee, Hyo-Jin Kim, Myoung-Hee Ahn, Jong-Yil Chai The Korean Journal of Parasitology.2008; 46(1): 45. CrossRef
Trends in parasitic diseases in the Republic of Korea Eun-Hee Shin, Sang-Mee Guk, Hyo-Jin Kim, Soon-Hyung Lee, Jong-Yil Chai Trends in Parasitology.2008; 24(3): 143. CrossRef
Use of GRA6-Derived Synthetic Polymorphic Peptides in an Immunoenzymatic Assay To Serotype
Toxoplasma gondii
in Human Serum Samples Collected from Three Continents
Susana Sousa, Daniel Ajzenberg, Manuel Vilanova, José Costa, Marie-Laure Dardé Clinical and Vaccine Immunology.2008; 15(9): 1380. CrossRef
The pond smelt Hypomesus olidus and minnow Zacco platypus were collected from the Soyang and Daechung Lakes in January 2003, and their metacercarial infections was examined by the muscle compression and artificial digestion techniques. In the Soyang Lake, 161 metacercariae of Clonorchis sinensis (0.35 per fish) were harvested from 459 pond smelts examined. Also, 13 metacercariae of C. sinensis (0.43 per fish), 1 of Metagonimus sp., 4 of Echinostoma sp., 148 of Centrocestus armatus and 44 unidentified species were collected from 30 minnows. In the Daechung Lake, 369 metacercariae of C. sinensis (3.69 per fish) and 51 unidentified species were recovered from 100 pond smelts. The metacercariae of C. sinensis were fed to experimental rats, in which the adult flukes were identified. The pond smelts and minnows collected from the Soyang and Daechung Lakes were verified to be the second intermediate hosts and the sources of human C. sinensis infection.
Citations
Citations to this article as recorded by
Research Note. Prevalence, protein analysis and possible preventive measures against zoonotic anisakid larvae isolated from marine Atherina fish M. Samir, M. A. Amin, A. O. Hassan, A. M. Merwad, M. A. I. Awadallah Helminthologia.2015; 52(4): 375. CrossRef
Prevalence ofClonorchis sinensisMetacercariae in Freshwater Fish from Three Latitudinal Regions of the Korean Peninsula Shin-Hyeong Cho, Woon-Mok Sohn, Byoung-Kuk Na, Tong-Soo Kim, Yoon Kong, Keeseon Eom, Won-Seok Seok, Taejoon Lee The Korean Journal of Parasitology.2011; 49(4): 385. CrossRef
Epidemiological investigation of Clonorchis sinensis infection in freshwater fishes in the Pearl River Delta Daixiong Chen, Jieyun Chen, Ji Huang, Xueying Chen, Dana Feng, Baofang Liang, Yuchuan Che, Xiaodan Liu, Cuihua Zhu, Xiaomin Li, Haoxian Shen Parasitology Research.2010; 107(4): 835. CrossRef
Scientific Opinion on risk assessment of parasites in fishery products
Discovery of Opisthorchis viverrini metacercariae in freshwater fish in southern Cambodia Sarun Touch, Chalit Komalamisra, Prayong Radomyos, Jitra Waikagul Acta Tropica.2009; 111(2): 108. CrossRef
Life cycle stages of heterophyid trematodes in Vietnamese freshwater fishes traced by molecular and morphometric methods Jakob Skov, Per W. Kania, Anders Dalsgaard, Thomas R. Jørgensen, Kurt Buchmann Veterinary Parasitology.2009; 160(1-2): 66. CrossRef
Infection Status of Freshwater Fish with Metacercariae of Clonorchis sinensis in Korea Eun-Min Kim, Jae-Lip Kim, Sung Yil Choi, Jae-Whan Kim, Siwon Kim, Min-Ho Choi, Young Mee Bae, Soon-Hyung Lee, Sung-Tae Hong The Korean Journal of Parasitology.2008; 46(4): 247. CrossRef
Infection status of pond smelts, Hypomesus olidus, and other freshwater fishes with trematode metacercariae in 6 large lakes Shin-Hyeong Cho, Woon-Mok Sohn, Sung-Shik Shin, Hyeon-Je Song, Taek-Gyun Choi, Chang-Mi Oh, Yoon Kong, Tong-Soo Kim The Korean Journal of Parasitology.2006; 44(3): 243. CrossRef
Clonorchiasis: a key foodborne zoonosis in China Zhao-Rong Lun, Robin B Gasser, De-Hua Lai, An-Xing Li, Xing-Quan Zhu, Xing-Bing Yu, Yue-Yi Fang The Lancet Infectious Diseases.2005; 5(1): 31. CrossRef
Fish-borne parasitic zoonoses: Status and issues Jong-Yil Chai, K. Darwin Murrell, Alan J. Lymbery International Journal for Parasitology.2005; 35(11-12): 1233. CrossRef
First
Prev
