Parasites, Hosts and Diseases

"Yuan Liu"

Original Article

Validation of Reference Genes for Quantitative Real-Time PCR in Bovine PBMCs Transformed and Non-transformed by Theileria annulata: Hongxi Zhao, Junlong Liu, Youquan Li, Congshan Yang, Shuaiyang Zhao, Juan Liu, Aihong Liu, Guangyuan Liu, Hong Yin, Guiquan Guan, Jianxun Luo; Korean J Parasitol 2016;54(1):39-46.
Published online February 26, 2016; DOI: https://doi.org/10.3347/kjp.2016.54.1.39

Abstract
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Theileria annulata is a tick-borne intracellular protozoan parasite that causes tropical theileriosis, a fatal bovine lymphoproliferative disease. The parasite predominantly invades bovine B lymphocytes and macrophages and induces host cell transformation by a mechanism that is not fully comprehended. Analysis of signaling pathways by quantitative real-time PCR (qPCR) could be a highly efficient means to understand this transformation mechanism. However, accurate analysis of qPCR data relies on selection of appropriate reference genes for normalization, yet few papers on T. annulata contain evidence of reference gene validation. We therefore used the geNorm and NormFinder programs to evaluate the stability of 5 candidate reference genes; 18S rRNA, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ACTB (β-actin), PRKG1 (protein kinase cGMP-dependent, type I) and TATA box binding protein (TBP). The results showed that 18S rRNA was the reference gene most stably expressed in bovine PBMCs transformed and non-transformed with T. annulata, followed by GAPDH and TBP. While 18S rRNA and GAPDH were the best combination, these 2 genes were chosen as references to study signaling pathways involved in the transformation mechanism of T. annulata.

Citations

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Virulence Is More than Adhesion and Invasion Ability, an In Vitro Cell Infection Assay of Bovine Mycoplasma spp.
Elhem Yacoub, Daniel Kos, Murray Jelinski
Microorganisms.2025; 13(3): 632. CrossRef
A Theileria annulata parasite with a single mutation, methionine 128 to isoleucine (M128I), in cytochrome B is resistant to buparvaquone
Shahin Tajeri, Debasish Chattopadhyay, Gordon Langsley, Ard M. Nijhof, Vikrant Sudan
PLOS ONE.2024; 19(4): e0299002. CrossRef
Traditional and emerging Fusarium mycotoxins disrupt homeostasis of bovine mammary cells by altering cell permeability and innate immune function
Ran Xu, Umesh K. Shandilya, Alexandros Yiannikouris, Niel A. Karrow
Animal Nutrition.2023; 12: 388. CrossRef
Investigation of Anaplasma marginale, Babesia bovis, Babesia bigemina and Trypanosoma vivax in the brain and spleen of dairy cows of Rio Grande do Sul
Melânia Lazzari Rigo, Kauê Rodriguez Martins, Yan Wahast Islabão, Alexia Brauner de Mello, Marjorie de Giacometi, Rodrigo Casquero Cunha, Monique Tomazele Rovani, Camila Belmonte Oliveira
Semina: Ciências Agrárias.2023; 44(6): 2063. CrossRef
1,25‐Dihydroxyvitamin D3 potentiates the innate immune response of peripheral blood mononuclear cells from Japanese Black cattle
Youki Oyamada, Ei'ichi Iizasa, Amane Usa, Konosuke Otomaru
Animal Science Journal.2023;[Epub] CrossRef
Internal reference genes with the potential for normalizing quantitative PCR results for oral fluid specimens
Ting-Yu Cheng, Jeffrey J. Zimmerman, Luis G. Giménez-Lirola
Animal Health Research Reviews.2022; 23(2): 147. CrossRef
Genetic diversity of Siberian bovine coronavirus isolates (Coronaviridae: Coronavirinae: Betacoronavirus-1: Bovine-Like coronaviruses)
Alexander G. Glotov, Aleksej V. Nefedchenko, Anton G. Yuzhakov, Svetlana V. Koteneva, Tatyana I. Glotova, Alina K. Komina, Nikita Yu. Krasnikov
Problems of Virology.2022; 67(6): 465. CrossRef
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Inhibition monitoring in veterinary molecular testing
Lifang Yan, Kathy L. Toohey-Kurth, Beate M. Crossley, Jianfa Bai, Amy L. Glaser, Rebecca L. Tallmadge, Laura B. Goodman
Journal of Veterinary Diagnostic Investigation.2020; 32(6): 758. CrossRef
Development and testing of the real-time polymerase chain reaction for identification and quantitative determination of the bovine respiratory syncytial virus
A.V. Nefedchenko, A.G. Glotov, S.V. Koteneva, T.I. Glotova
Molecular Genetics Microbiology and Virology (Russian version).2020; 38(3): 145. CrossRef
Developing and Testing a Real-Time Polymerase Chain Reaction to Identify and Quantify Bovine Respiratory Syncytial Viruses
A. V. Nefedchenko, A. G. Glotov, S. V. Koteneva, T. I. Glotova
Molecular Genetics, Microbiology and Virology.2020; 35(3): 168. CrossRef
Subacute ruminal acidosis affects fermentation and endotoxin concentration in the rumen and relative expression of the CD14/TLR4/MD2 genes involved in lipopolysaccharide systemic immune response in dairy cows
B. Stefanska, W. Człapa, E. Pruszynska-Oszmałek, D. Szczepankiewicz, V. Fievez, J. Komisarek, K. Stajek, W. Nowak
Journal of Dairy Science.2018; 101(2): 1297. CrossRef
Selection and evaluation of housekeeping genes as endogenous controls for quantification of mRNA transcripts in Theileria parva using quantitative real-time polymerase chain reaction (qPCR)
Teboho N. Tsotetsi, Nicola E. Collins, Marinda C. Oosthuizen, Kgomotso P. Sibeko-Matjila, Gordon Langsley
PLOS ONE.2018; 13(5): e0196715. CrossRef

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Brief Communication

Morphological Characteristics of Normal and Gynandromorphic Hyalomma asiaticum Schulze and Schlottke, 1930: Ze Chen, You-quan Li, Qiao-Yun Ren, Jin Luo, Yonghong Hu, Kai Li, Guang-Yuan Liu, Jian-xun Luo, Jingze Liu, Hong Yin; Korean J Parasitol 2015;53(3):361-364.
Published online June 30, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.3.361

Abstract
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Gynandromorphic ticks are extremely rare, and often attract parasitologists’ attention. During our examination of tick specimens, an engorged gynandromorph of Hyalomma asiaticum was noticed. This is the first record of gynandromorphic ticks from China. In this study, several important morphological structures of normal and gynandromorphic H. asiaticum were analyzed. Comparing to the normal H. asiaticum, the gynandromorphic specimen was a typical bipartite protogynander. Its right side showed normal female characteristics, whereas the left side had normal male traits. Different from other gynandromorphic ticks containing 1 anus, this tick reported here had 2 complete anuses, and the anus of the male part had a single adanal plate.

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A case of gynandromorphism in Hyalomma anatolicum (Ixodida: Ixodidae)
Yan Liu, Xiaoyun Mi, Bingjie Wang, Jun Wu, Wenwen He, Tingxiang Luo, Depeng Yang, Zhengxiang Hu, Lu Gan, Mingdalai Nuo, Huizhen Zheng, Ercha Hu, Qingyong Guo
Experimental and Applied Acarology.2023; 91(1): 133. CrossRef
Morphological abnormalities in Hyalomma dromedarii and Hyalomma rufipes (Acari: Ixodidae) collected from dromedary camels (Camelus dromedarius) in Aswan, Egypt
Mohammed Okely, Deon K. Bakkes, Lidia Chitimia-Dobler
Experimental and Applied Acarology.2022; 88(2): 225. CrossRef
Sequencing of complete mitochondrial genomes confirms synonymization of Hyalomma asiaticum asiaticum and kozlovi, and advances phylogenetic hypotheses for the Ixodidae
Zhi-Qiang Liu, Yan-Feng Liu, Nuer Kuermanali, Deng-Feng Wang, Shi-Jun Chen, Hui-Ling Guo, Li Zhao, Jun-Wei Wang, Tao Han, Yuan-Zhi Wang, Jie Wang, Chen-Feng Shen, Zhuang-Zhi Zhang, Chuang-Fu Chen, Ulrike Gertrud Munderloh
PLOS ONE.2018; 13(5): e0197524. CrossRef
Abnormal Development of Haemaphysalis qinghaiensis (Acari: Ixodidae)
Qiaoyun Ren, Ze Chen, Jin Luo, Guangyuan Liu, Guiquan Guan, Hong Yin, Jianxun Luo
Journal of Insect Science.2016;[Epub] CrossRef

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Original Article

Sequence Variation in Superoxide Dismutase Gene of Toxoplasma gondii among Various Isolates from Different Hosts and Geographical Regions: Shuai Wang, Aiping Cao, Xun Li, Qunli Zhao, Yuan Liu, Hua Cong, Shenyi He, Huaiyu Zhou; Korean J Parasitol 2015;53(3):253-258.
Published online June 30, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.3.253

Abstract
PDF

Toxoplasma gondii, an obligate intracellular protozoan parasite of the phylum Apicomplexa, can infect all warm-blooded vertebrates, including humans, livestock, and marine mammals. The aim of this study was to investigate whether superoxide dismutase (SOD) of T. gondii can be used as a new marker for genetic study or a potential vaccine candidate. The partial genome region of the SOD gene was amplified and sequenced from 10 different T. gondii isolates from different parts of the world, and all the sequences were examined by PCR-RFLP, sequence analysis, and phylogenetic reconstruction. The results showed that partial SOD gene sequences ranged from 1,702 bp to 1,712 bp and A + T contents varied from 50.1% to 51.1% among all examined isolates. Sequence alignment analysis identified total 43 variable nucleotide positions, and these results showed that 97.5% sequence similarity of SOD gene among all examined isolates. Phylogenetic analysis revealed that these SOD sequences were not an effective molecular marker for differential identification of T. gondii strains. The research demonstrated existence of low sequence variation in the SOD gene among T. gondii strains of different genotypes from different hosts and geographical regions.

Citations

Citations to this article as recorded by

Superoxide Dismutases in Immune Regulation and Infectious Diseases
Tong Liu, Jiajin Shang, Qijun Chen
Antioxidants.2025; 14(7): 809. CrossRef
Immunization with a DNA vaccine encoding Toxoplasma gondii Superoxide dismutase (TgSOD) induces partial immune protection against acute toxoplasmosis in BALB/c mice
Yuan Liu, Aiping Cao, Yawen Li, Xun Li, Hua Cong, Shenyi He, Huaiyu Zhou
BMC Infectious Diseases.2017;[Epub] CrossRef

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Mini Review

Serine Proteases of Parasitic Helminths: Yong Yang, Yun jun Wen, Ya Nan Cai, Isabelle Vall?e, Pascal Boireau, Ming Yuan Liu, Shi Peng Cheng; Korean J Parasitol 2015;53(1):1-11.
Published online February 27, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.1.1

Abstract
PDF

Serine proteases form one of the most important families of enzymes and perform significant functions in a broad range of biological processes, such as intra- and extracellular protein metabolism, digestion, blood coagulation, regulation of development, and fertilization. A number of serine proteases have been identified in parasitic helminths that have putative roles in parasite development and nutrition, host tissues and cell invasion, anticoagulation, and immune evasion. In this review, we described the serine proteases that have been identified in parasitic helminths, including nematodes (Trichinella spiralis, T. pseudospiralis, Trichuris muris, Anisakis simplex, Ascaris suum, Onchocerca volvulus, O. lienalis, Brugia malayi, Ancylostoma caninum, and Steinernema carpocapsae), cestodes (Spirometra mansoni, Echinococcus granulosus, and Schistocephalus solidus), and trematodes (Fasciola hepatica, F. gigantica, and Schistosoma mansoni). Moreover, the possible biological functions of these serine proteases in the endogenous biological phenomena of these parasites and in the host-parasite interaction were also discussed.

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Intranasal immunization with recombinant Trichinella spiralis serine protease elicits protective immunity in BALB/c mice
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Cloning, expression and characterization of a novel alkaline serine protease gene from native Iranian Bacillus sp.; a producer of protease for use in livestock
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Hybrid Assembly of the Genome of the Entomopathogenic Nematode Steinernema carpocapsae Identifies the X-Chromosome
Lorrayne Serra, Marissa Macchietto, Aide Macias-Muñoz, Cassandra Joan McGill, Isaryhia Maya Rodriguez, Bryan Rodriguez, Rabi Murad, Ali Mortazavi
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Complex insight on microanatomy of larval “human broad tapeworm” Dibothriocephalus latus (Cestoda: Diphyllobothriidea)
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Molecular cloning and characterization of leucine aminopeptidase gene from Taenia pisiformis
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SmSP2: A serine protease secreted by the blood fluke pathogen Schistosoma mansoni with anti-hemostatic properties
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Molecular characterization of a putative serine protease from Trichinella spiralis and its elicited immune protection
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Silent Witness: Dual-Species Transcriptomics Reveals Epithelial Immunological Quiescence to Helminth Larval Encounter and Fostered Larval Development
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Expert Review of Proteomics.2017; 14(2): 171. CrossRef
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Allergologia et Immunopathologia.2017; 45(6): 579. CrossRef
Proteolytic enzymes and their inhibitors in cestodes
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Karina M. Monteiro, Karina R. Lorenzatto, Jeferson C. de Lima, Guilherme B. dos Santos, Sabine Förster, Gabriela P. Paludo, Paulo C. Carvalho, Klaus Brehm, Henrique B. Ferreira
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Proteomic Analysis of Trichinella spiralis Adult Worm Excretory-Secretory Proteins Recognized by Sera of Patients with Early Trichinellosis
Zhong Q. Wang, Ruo D. Liu, Ge G. Sun, Yan Y. Song, Peng Jiang, Xi Zhang, Jing Cui
Frontiers in Microbiology.2017;[Epub] CrossRef
Immunoproteomic analysis of the excretory-secretory products of Trichinella pseudospiralis adult worms and newborn larvae
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