We have reported that a 24 kDa protein (22U homologous; As22U) of Anisakis simplex larvae could elicit several Th2-related chemokine gene expressions in the intestinal epithelial cell line which means that As22U may play a role as an allergen. In order to determine the contribution of As22U to allergic reactions, we treated mice with 6 times intra-nasal application of recombinant As22U (rAs22U). In the group challenged with rAs22U and ovalbumin (OVA), the number of eosinophils in the bronchial alveolar lavage fluid (BALF) was significantly increased, as compared to the group receiving only OVA. In addition, mice treated with rAs22U and OVA showed significantly increased airway hyperresponsiveness. Thus, severe inflammation around the airway and immune cell recruitment was observed in mice treated with rAs22U plus OVA. The levels of IL-4, IL-5, and IL-13 cytokines in the BALF increased significantly after treatment with rAs22U and OVA. Similarly, the levels of anti-OVA specific IgE and IgG1 increased in mice treated with rAs22U and OVA, compared to those treated only with OVA. The Gro-α (CXCL1) gene expression in mouse lung epithelial cells increased instantly after treatment with rAs22U, and allergy-specific chemokines eotaxin (CCL11) and thymus-and-activation-regulated-chemokine (CCL17) gene expressions significantly increased at 6 hr after treatment. In conclusion, rAs22U may induce airway allergic inflammation, as the result of enhanced Th2 and Th17 responses.
Citations
Citations to this article as recorded by
Anisakis pegreffii Extract Induces Airway Inflammation with Airway Remodeling in a Murine Model System Jun Ho Choi, Ju Yeong Kim, Myung-hee Yi, Myungjun Kim, Tai-Soon Yong, Kalman Imre BioMed Research International.2021;[Epub] CrossRef
Allergen-like Molecules from Parasites Ju Yeong Kim, Myung-Hee Yi, Tai-Soon Yong Current Protein & Peptide Science.2020; 21(2): 186. CrossRef
Research Note. Prevalence, protein analysis and possible preventive measures against zoonotic anisakid larvae isolated from marine Atherina fish M. Samir, M. A. Amin, A. O. Hassan, A. M. Merwad, M. A. I. Awadallah Helminthologia.2015; 52(4): 375. CrossRef
Allergenicity of two Anisakis simplex allergens evaluated in vivo using an experimental mouse model Min Kyoung Cho, Mi Kyung Park, Shin Ae Kang, Maria Luisa Caballero, Teresa Perez-Pinar, Rosa Rodriguez-Perez, Mee Sun Ock, Hee Jae Cha, Yeon Chul Hong, Hak Sun Yu Experimental Parasitology.2014; 146: 71. CrossRef
Alteration of Cytokine Production during Visceral Larva Migrans by Toxascaris leonina in Mice Shin Ae Kang, Mi-Kyung Park, Min Kyoung Cho, Hak Sun Yu The Korean Journal of Parasitology.2013; 51(5): 583. CrossRef
In order to get a better understanding of the role of protease-activated receptor 2 (PAR2) in type 2 helper T (Th2) cell responses against Trichinella spiralis infection, we analyzed Th2 responses in T. spiralis-infected PAR2 knockout (KO) mice. The levels of the Th2 cell-secreted cytokines, IL-4, IL-5, and IL-13 were markedly reduced in the PAR2 KO mice as compared to the wild type mice following infection with T. spiralis. The serum levels of parasite-specific IgE increased significantly in the wild type mice as the result of T. spiralis infection, but this level was not significantly increased in PAR2 KO mice. The expression level of thymic stromal lymphopoietin, IL-25, and eotaxin gene (the genes were recently known as Th2 response initiators) of mouse intestinal epithelial cells were increased as the result of treatment with T. spiralis excretory-secretory proteins. However, the expression of these chemokine genes was inhibited by protease inhibitor treatments. In conclusion, PAR2 might involve in Th2 responses against T. spiralis infection.
Citations
Citations to this article as recorded by
Innate Immune Recognition of Helminths: TLRs and Beyond Camila de Almeida Lopes, Linda Djune Yemeli, Pedro Gazzinelli‐Guimaraes Parasite Immunology.2025;[Epub] CrossRef
A novel trypsin of Trichinella spiralis mediates larval invasion of gut epithelium via binding to PAR2 and activating ERK1/2 pathway Lu Lu Han, Qi Qi Lu, Wen Wen Zheng, Yang Li Li, Yan Yan Song, Xin Zhuo Zhang, Shao Rong Long, Ruo Dan Liu, Zhong Quan Wang, Jing Cui, Krystyna Cwiklinski PLOS Neglected Tropical Diseases.2024; 18(1): e0011874. CrossRef
Pathogenesis ofBrucellaepididymoorchitis-game ofBrucelladeath Jiuwang Yu, Sha Li, Lu Wang, Zhiheng Dong, Lengge Si, Lidao Bao, Lan Wu Critical Reviews in Microbiology.2022; 48(1): 96. CrossRef
Cytokines and beyond: Regulation of innate immune responses during helminth infection Oyebola O. Oyesola, Simon P. Früh, Lauren M. Webb, Elia D. Tait Wojno Cytokine.2020; 133: 154527. CrossRef
Animal Models for Functional Gastrointestinal Disorders Alison Accarie, Tim Vanuytsel Frontiers in Psychiatry.2020;[Epub] CrossRef
Adoptive transfer of Trichinella spiralis-activated macrophages can ameliorate both Th1- and Th2-activated inflammation in murine models Shin Ae Kang, Mi-Kyung Park, Sang Kyun Park, Jun Ho Choi, Da In Lee, So Myong Song, Hak Sun Yu Scientific Reports.2019;[Epub] CrossRef
The role of rare innate immune cells in Type 2 immune activation against parasitic helminths LAUREN M. WEBB, ELIA D. TAIT WOJNO Parasitology.2017; 144(10): 1288. CrossRef
The induction of the collagen capsule synthesis by Trichinella spiralis is closely related to protease-activated receptor 2 Mi Kyung Park, Min Kyoung Cho, Shin Ae Kang, Bo Young Kim, Hak Sun Yu Veterinary Parasitology.2016; 230: 56. CrossRef
Human Helminths and Allergic Disease: The Hygiene Hypothesis and Beyond Thomas B. Nutman, Helton C. Santiago The American Journal of Tropical Medicine and Hygiene.2016; 95(4): 746. CrossRef
The role of infection in the pathogenesis of allergodermatoses E. V. Svirshchevskaya, E. V. Matushevskaya, D. B. Chudakov, Yu. I. Matushevskaya Klinicheskaya dermatologiya i venerologiya.2015; 14(2): 4. CrossRef
Genetic regulation of immunoglobulin E level in different pathological states: integration of mouse and human genetics Elena S. Gusareva, Iryna Kurey, Igor Grekov, Marie Lipoldová Biological Reviews.2014; 89(2): 375. CrossRef
Thymic stromal lymphopoietin and atopic diseases J.M. Leyva-Castillo, M. Li Revue Française d'Allergologie.2014; 54(5): 364. CrossRef
Parasitic Nematode-Induced CD4+Foxp3+T Cells Can Ameliorate Allergic Airway Inflammation Shin Ae Kang, Mi-Kyung Park, Min Kyoung Cho, Sang Kyun Park, Min Seong Jang, Bo-Gie Yang, Myoung Ho Jang, Dong-Hee Kim, Hak Sun Yu, Edward Mitre PLoS Neglected Tropical Diseases.2014; 8(12): e3410. CrossRef
Coinfection with Clonorchis sinensis modulates murine host response against Trichinella spiralis infection Ying Chen, Bo Huang, Shiguang Huang, Xinbing Yu, Yonglong Li, Wenjian Song, Yongxiang Li, Fangli Lu Parasitology Research.2013; 112(9): 3167. CrossRef
Alteration of Cytokine Production during Visceral Larva Migrans by Toxascaris leonina in Mice Shin Ae Kang, Mi-Kyung Park, Min Kyoung Cho, Hak Sun Yu The Korean Journal of Parasitology.2013; 51(5): 583. CrossRef
Toward Drugs for Protease-Activated Receptor 2 (PAR2) Mei-Kwan Yau, Ligong Liu, David P. Fairlie Journal of Medicinal Chemistry.2013; 56(19): 7477. CrossRef
IL-32-PAR2 axis is an innate immunity sensor providing alternative signaling for LPS-TRIF axis Masanori Nakayama, Yasuo Niki, Toshiki Kawasaki, Yuki Takeda, Hiroyasu Ikegami, Yoshiaki Toyama, Takeshi Miyamoto Scientific Reports.2013;[Epub] CrossRef
Protease‐activated receptor‐2 signalling by tissue factor on dendritic cells suppresses antigen‐specific CD4+ T‐cell priming Seema Shrivastava, Liang Ma, El‐Li Tham, John H. McVey, Daxin Chen, Anthony Dorling Immunology.2013; 139(2): 219. CrossRef
Molecular characterization of 45kDa aspartic protease of Trichinella spiralis Jong Nam Park, Sang Kyun Park, Min Kyoung Cho, Mi-Kyung Park, Shin Ae Kang, Dong-Hee Kim, Hak Sun Yu Veterinary Parasitology.2012; 190(3-4): 510. CrossRef
Regulation and dysregulation of innate immunity by NFAT signaling downstream of pattern recognition receptors (PRRs) Ivan Zanoni, Francesca Granucci European Journal of Immunology.2012; 42(8): 1924. CrossRef
First
Prev
