Parasites, Hosts and Diseases

"antibiotic"

Original Article

Ampicillin treated German cockroach extract leads to reduced inflammation in human lung cells and a mouse model of Asthma: Seogwon Lee, Myung-Hee Yi, Yun Soo Jang, Jun Ho Choi, Myungjun Kim, Soo Lim Kim, Tai-Soon Yong, Ju Yeong Kim; Parasites Hosts Dis 2023;61(1):60-71.
Published online February 22, 2023; DOI: https://doi.org/10.3347/PHD.22147

Cockroaches can cause allergic sensitization in humans via contact with their feces or frass. Antibiotics can affect concentration of major allergen and total bacteria production in German cockroaches (Blattella germanica). This study examined the ability of antibiotic-treated German cockroaches to induce allergic airway inflammation and the effect of antibiotics on their lipopolysaccharide and Bla g1, 2, and 5 expression levels. Specifically, we measured the ability of German cockroach extract (with or without prior antibiotic exposure) to induce allergic inflammation in human bronchial epithelial cells and a mouse model of asthma. Bacterial 16S rRNA and lipopolysaccharide levels were lower in ampicillin-treated cockroaches than in the control group. The Bla g1, Bla g2, and Bla g5 expression in ampicillin-treated cockroaches decreased at both the protein and RNA levels. In human bronchial epithelial cell lines BEAS-2B exposed to the ampicillin-treated extract, expression levels of interleukin-6 and interleukin-8 were lower than that in the control group. The total cell count and eosinophil count in bronchoalveolar lavage fluid was also lower in mice exposed to the ampicillin-treated extract than in those exposed to normal cockroach extract. Mouse lung histopathology showed reduced immune cell infiltration and mucus production in the ampicillin group. Our results showed that ampicillin treatment reduced the symbiont bacterial population and major allergen levels in German cockroaches, leading to reduced airway inflammation in mice. These results can facilitate the preparation of protein extracts for immunotherapy or diagnostics applications.

Citations

Citations to this article as recorded by

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Invasive indoor pests under the microbiological lens: bacterial and viral diversity from local to global scales in bed bugs and cockroaches
Jose E Pietri, Maureen Laroche
Current Opinion in Insect Science.2025; 69: 101344. CrossRef
Multi-omics of cockroaches infected with Salmonella Typhimurium identifies molecular signatures of vector colonization
Diing DM Agany, Eduardo A. Callegari, Maria D. Paez, Jose E. Pietri
BMC Genomics.2025;[Epub] CrossRef
Microbiome of laboratory‐reared and environmentally collected cockroaches
Sohyeon Yun, Jun Ho Choi, Singeun Oh, Myungjun Kim, Myung‐hee Yi, Dongjun Kang, Yun Soo Jang, In‐Yong Lee, Tai‐Soon Yong, Juan Kim, Heung Chul Kim, Jae Rok Lee, Ju Yeong Kim
Entomological Research.2024;[Epub] CrossRef

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Brief Communication

Glutathione Reductase and Thioredoxin Reductase: Novel Antioxidant Enzymes from Plasmodium berghei: Gaurav Kapoor, Harjeet Singh Banyal; Korean J Parasitol 2009;47(4):421-424.
Published online December 1, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.4.421

Abstract
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Malaria parasites adapt to the oxidative stress during their erythrocytic stages with the help of vital thioredoxin redox system and glutathione redox system. Glutathione reductase and thioredoxin reductase are important enzymes of these redox systems that help parasites to maintain an adequate intracellular redox environment. In the present study, activities of glutathione reductase and thioredoxin reductase were investigated in normal and Plasmodium berghei-infected mice red blood cells and their fractions. Activities of glutathione reductase and thioredoxin reductase in P. berghei-infected host erythrocytes were found to be higher than those in normal host cells. These enzymes were mainly confined to the cytosolic part of cell-free P. berghei. Full characterization and understanding of these enzymes may promise advances in chemotherapy of malaria.

Citations

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Free Radical Biology and Medicine.2011; 50(11): 1659. CrossRef