Parasites, Hosts and Diseases

"cDNA library"

Brief Communications

Expression and Characterization of α-Methylacyl CoA Racemase from Anisakis simplex Larvae: Bong Jin Kim, Sun Mi Kim, Min Kyung Cho, Hak Sun Yu, Yong Seok Lee, Hee Jae Cha, Meesun Ock; Korean J Parasitol 2012;50(2):165-171.
Published online May 24, 2012; DOI: https://doi.org/10.3347/kjp.2012.50.2.165

Abstract
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Larval excretory-secretory products of Anisakis simplex are known to cause allergic reactions in humans. A cDNA library of A. simplex 3rd-stage larvae (L3) was immunoscreened with polyclonal rabbit serum raised against A. simplex L3 excretory-secretory products to identify an antigen that elicits the immune response. One cDNA clone, designated as α-methylacyl CoA racemase (Amacr) contained a 1,412 bp cDNA transcript with a single open reading frame that encoded 418 amino acids. A. simplex Amacr showed a high degree of homology compared to Amacr orthologs from other species. Amacr mRNA was highly and constitutively expressed regardless of temperature (10-40℃) and time (24-48 hr). Immunohistochemical analysis revealed that Amacr was expressed mainly in the ventriculus of A. simplex larvae. The Amacr protein produced in large quantities from the ventriculus is probably responsible for many functions in the development and growth of A. simplex larvae.

Citations

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The mitochondrial genome ofAncylostoma tubaeformefrom cats in China
X.L. Shi, Y.Q. Fu, A.Y. Abdullahi, M.W. Wang, F. Yang, X.G. Yu, W.D. Pan, X.X. Yan, J.X. Hang, P. Zhang, G.Q. Li
Journal of Helminthology.2018; 92(1): 22. CrossRef
α-Methylacyl-CoA racemase (AMACR): Metabolic enzyme, drug metabolizer and cancer marker P504S
Matthew D. Lloyd, Maksims Yevglevskis, Guat Ling Lee, Pauline J. Wood, Michael D. Threadgill, Timothy J. Woodman
Progress in Lipid Research.2013; 52(2): 220. CrossRef

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Cloning of the novel putative apoptosis-related gene of Spirometra erinacei (Order Pseudophyllidea): Soo-Ung Lee, Sun Huh; Korean J Parasitol 2006;44(3):233-237.
Published online September 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.3.233

Abstract
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We postulated that apolysis was processed in accordance with apoptotic changes occurring in a cestode, Spirometra erinacei (Pseudophyllidea). We cloned the novel putative apoptosis-associated gene from S. erinacei via screening of a S. erinacei cDNA library with a ced-3 gene (activator of apoptosis) probe from Caenorhabditis elegans. We identified a 261-bp cDNA sequence, which encodes for an 86-amino acid protein. The cloned gene expression was observed in the neck and gravid proglottids via Northern blotting, using cloned cDNA inserts as probes, but the clone was not expressed in any of other tissues. We suggest that this gene may be involved in the apolysis of S. erinacei during normal tissue development and differentiation in cestode parasites.

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The genome of the sparganosis tapeworm Spirometra erinaceieuropaeiisolated from the biopsy of a migrating brain lesion
Hayley M Bennett, Hoi Ping Mok, Effrossyni Gkrania-Klotsas, Isheng J Tsai, Eleanor J Stanley, Nagui M Antoun, Avril Coghlan, Bhavana Harsha, Alessandra Traini, Diogo M Ribeiro, Sascha Steinbiss, Sebastian B Lucas, Kieren SJ Allinson, Stephen J Price, Thom
Genome Biology.2014;[Epub] CrossRef

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Original Article

Expressed sequence tags analysis of Blattella germanica: Hyang Suk Chung, Tai Hyun Yu, Bong Jin Kim, Sun Mi Kim, Joo Yeong Kim, Hak Sun Yu, Hae Jin Jeong, Mee Sun Ock; Korean J Parasitol 2005;43(4):149-156.
Published online December 20, 2005; DOI: https://doi.org/10.3347/kjp.2005.43.4.149

Abstract
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Four hundred and sixty five randomly selected clones from a cDNA library of Blattella germanica were partially sequenced and searched using BLAST as a means of analyzing the transcribed sequences of its genome. A total of 363 expressed sequence tags (ESTs) were generated from 465 clones after editing and trimming the vector and ambiguous sequences. About 42% (154/363) of these clones showed significant homology with other data base registered genes. These new B. germanica genes constituted a broad range of transcripts distributed among ribosomal proteins, energy metabolism, allergens, proteases, protease inhibitors, enzymes, translation, cell signaling pathways, and proteins of unknown function. Eighty clones were not well-matched by database searches, and these represent new B. germanica-specific ESTs. Some genes which drew our attention are discussed. The information obtained increases our understanding of the B. germanica genome.

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