Parasites, Hosts and Diseases

"complement"

Original Articles

Mapping of the Complement C9 Binding Region on Clonorchis sinensis Paramyosin: Jung-Mi Kang, Hương Giang Lê, Tuấn Cường Võ, Won Gi Yoo, Woon-Mok Sohn, Byoung-Kuk Na; Korean J Parasitol 2022;60(4):255-259.
Published online August 24, 2022; DOI: https://doi.org/10.3347/kjp.2022.60.4.255

Abstract
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Heliminthic paramyosin is a multifunctional protein that not only acts as a structural protein in muscle layers but as an immune-modulatory molecule interacting with the host immune system. Previously, we found that paramyosin from Clonorchis sinensis (CsPmy) is bound to human complement C9 protein (C9). To analyze the C9 binding region on CsPmy, overlapping recombinant fragments of CsPmy were produced and their binding activity to human C9 was investigated. The fragmental expression of CsPmy and C9 binding assays revealed that the C9 binding region was located at the C-terminus of CsPmy. Further analysis of the C-terminus of CsPmy to narrow the C9 binding region on CsPmy indicated that the region flanking⁷³¹Leu–⁷⁸⁰ Leu was a potent C9 binding region. The CsPmy fragments corresponding to the region effectively inhibited human C9 polymerization. These results provide a precise molecular basis for CsPmy as a potent immunomodulator to evade host immune defenses by inhibiting complement attack.

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Transmission-Blocking Vaccines against Schistosomiasis Japonica
Chika P. Zumuk, Malcolm K. Jones, Severine Navarro, Darren J. Gray, Hong You
International Journal of Molecular Sciences.2024; 25(3): 1707. CrossRef
What about the Cytoskeletal and Related Proteins of Tapeworms in the Host’s Immune Response? An Integrative Overview
Diana G. Ríos-Valencia, Javier Ambrosio, Rocío Tirado-Mendoza, Julio César Carrero, Juan Pedro Laclette
Pathogens.2023; 12(6): 840. CrossRef

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Molecular Cloning and Characterization of a Paramyosin from Clonorchis sinensis: Tae-Joon Park, Jung-Mi Kang, Byoung-Kuk Na, Woon-Mok Sohn; Korean J Parasitol 2009;47(4):359-367.
Published online December 1, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.4.359

Abstract
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Paramyosin is a myofibrillar protein present in helminth parasites and plays multifunctional roles in host-parasite interactions. In this study, we identified the gene encoding paramyosin of Clonorchis sinensis (CsPmy) and characterized biochemical and immunological properties of its recombinant protein. CsPmy showed a high level of sequence identity with paramyosin from other helminth parasites. Recombinant CsPmy (rCsPmy) expressed in bacteria had an approximate molecular weight of 100 kDa and bound both human collagen and complement 9. The protein was constitutively expressed in various developmental stages of the parasite. Imunofluorescence analysis revealed that CsPmy was mainly localized in the tegument, subtegumental muscles, and the muscle layer surrounding the intestine of the parasite. The rCsPmy showed high levels of positive reactions (74.6%, 56/75) against sera from patients with clonorchiasis. Immunization of experimental rats with rCsPmy evoked high levels of IgG production. These results collectively suggest that CsPmy is a multifunctional protein that not only contributes to the muscle layer structure but also to non-muscular functions in host-parasite interactions. Successful induction of host IgG production also suggests that CsPmy can be applied as a diagnostic antigen and/or vaccine candidate for clonorchiasis.

Citations

Citations to this article as recorded by

Among-population proteomic differences in Schistocephalus solidus based on excretory/secretory and total body protein predictions
Anni Wang, Daniel I. Bolnick
Parasites & Vectors.2025;[Epub] CrossRef
What about the Cytoskeletal and Related Proteins of Tapeworms in the Host’s Immune Response? An Integrative Overview
Diana G. Ríos-Valencia, Javier Ambrosio, Rocío Tirado-Mendoza, Julio César Carrero, Juan Pedro Laclette
Pathogens.2023; 12(6): 840. CrossRef
Current status of Clonorchis sinensis and clonorchiasis in Korea: epidemiological perspectives integrating the data from human and intermediate hosts
Won Gi Yoo, Woon-Mok Sohn, Byoung-Kuk Na
Parasitology.2022; 149(10): 1296. CrossRef
Mapping of the Complement C9 Binding Region on Clonorchis sinensis Paramyosin
Jung-Mi Kang, Hương Giang Lê, Tuấn Cường Võ, Won Gi Yoo, Woon-Mok Sohn, Byoung-Kuk Na
The Korean Journal of Parasitology.2022; 60(4): 255. CrossRef
Characterization of paramyosin protein structure and gene expression during myogenesis in Pacific oyster (Crassostrea gigas)
Huijuan Li, Qi Li, Hong Yu, Shaojun Du
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology.2021; 255: 110594. CrossRef
Clonorchis sinensis and clonorchiasis
Byoung-Kuk Na, Jhang Ho Pak, Sung-Jong Hong
Acta Tropica.2020; 203: 105309. CrossRef
Proteomic and Bioinformatic Investigations of Heat-Treated Anisakis simplex Third-Stage Larvae
Maciej Kochanowski, Mirosław Różycki, Joanna Dąbrowska, Aneta Bełcik, Jacek Karamon, Jacek Sroka, Tomasz Cencek
Biomolecules.2020; 10(7): 1066. CrossRef
Bacillus subtilis spore with surface display of paramyosin from Clonorchis sinensis potentializes a promising oral vaccine candidate
Hengchang Sun, Zhipeng Lin, Lu Zhao, Tingjin Chen, Mei Shang, Hongye Jiang, Zeli Tang, Xinyi Zhou, Mengchen Shi, Lina Zhou, Pengli Ren, Honglin Qu, Jinsi Lin, Xuerong Li, Jin Xu, Yan Huang, Xinbing Yu
Parasites & Vectors.2018;[Epub] CrossRef
Infection by the Helminth Parasite Fasciola hepatica Requires Rapid Regulation of Metabolic, Virulence, and Invasive Factors to Adjust to Its Mammalian Host
Krystyna Cwiklinski, Heather Jewhurst, Paul McVeigh, Tara Barbour, Aaron G. Maule, Jose Tort, Sandra M. O'Neill, Mark W. Robinson, Sheila Donnelly, John P. Dalton
Molecular & Cellular Proteomics.2018; 17(4): 792. CrossRef
Paramyosin of canine Onchocerca lupi: usefulness for the diagnosis of a neglected zoonotic disease
Bronwyn Campbell, Helder Cortes, Giada Annoscia, Alessio Giannelli, Antonio Parisi, Maria Stefania Latrofa, Filipe Dantas-Torres, Luís Cardoso, Domenico Otranto
Parasites & Vectors.2016;[Epub] CrossRef
Proteomic analysis of the excretory/secretory products and antigenic proteins of Echinococcus granulosus adult worms from infected dogs
Ying Wang, Di Xiao, Yujuan Shen, Xiuming Han, Fei Zhao, Xiaohong Li, Weiping Wu, Hejun Zhou, Jianzhong Zhang, Jianping Cao
BMC Veterinary Research.2015;[Epub] CrossRef
Mapping of the putative epitope domain of Clonorchis sinensis paramyosin (CsPmy) recognized by CsPmy-specific immunoglobulin G in sera of human clonorchiasis
Jung-Mi Kang, Hye-Lim Ju, Jinyoung Lee, Tae Im Kim, Shin-Hyeong Cho, Tong-Soo Kim, Woon-Mok Sohn, Byoung-Kuk Na
Molecular and Biochemical Parasitology.2015; 201(1): 66. CrossRef
Trichinella spiralis Paramyosin Binds Human Complement C1q and Inhibits Classical Complement Activation
Ran Sun, Xi Zhao, Zixia Wang, Jing Yang, Limei Zhao, Bin Zhan, Xinping Zhu, Elizabeth Angelica Leme Martins
PLOS Neglected Tropical Diseases.2015; 9(12): e0004310. CrossRef
The identification of antigenic proteins: 14-3-3 protein and propionyl-CoA carboxylase in Clonorchis sinensis
Myoung-Ro Lee, Yu-Jung Kim, Dae-Won Kim, Won Gi Yoo, Shin-Hyeong Cho, Kwang Yeon Hwang, Jung-Won Ju, Won-Ja Lee
Molecular and Biochemical Parasitology.2012; 182(1-2): 1. CrossRef
Molecular identification and characterization of leucine aminopeptidase 2, an excretory-secretory product of Clonorchis sinensis
Chuanhuan Deng, Jiufeng Sun, Xuerong Li, Lexun Wang, Xuchu Hu, Xiaoyun Wang, Wenjun Chen, Xiaoli Lv, Chi Liang, Wenfang Li, Yan Huang, Ran Li, Zhongdao Wu, Xinbing Yu, Jin Xu
Molecular Biology Reports.2012; 39(10): 9817. CrossRef
Synthetic peptide-targeted selection of phage display mimotopes highlights immunogenic features of α-helical vs non-helical epitopes of Taenia solium paramyosin: Implications for parasite- and host-protective roles of the protein
Karlen G. Gazarian, Carlos F. Solis, Tatiana G. Gazarian, Merrill Rowley, Juan P. Laclette
Peptides.2012; 34(1): 232. CrossRef
Identification and Characterization of Paramyosin from Cyst Wall of Metacercariae Implicated Protective Efficacy against Clonorchis sinensis Infection
Xiaoyun Wang, Wenjun Chen, Xiaoli Lv, Yanli Tian, Jingtao Men, Xifeng Zhang, Huali Lei, Chenhui Zhou, Fangli Lu, Chi Liang, Xuchu Hu, Jin Xu, Zhongdao Wu, Xuerong Li, Xinbing Yu, Erika Martins Braga
PLoS ONE.2012; 7(3): e33703. CrossRef
Progress on the transcriptomics of carcinogenic liver flukes of humans—Unique biological and biotechnological prospects
Neil D. Young, Aaron R. Jex, Cinzia Cantacessi, Bronwyn E. Campbell, Thewarach Laha, Woon-Mok Sohn, Banchob Sripa, Alex Loukas, Paul J. Brindley, Robin B. Gasser
Biotechnology Advances.2010; 28(6): 859. CrossRef

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Complement-mediated tail degradation of Neodiplostomum seoulense cercariae: Yun-Kyu Park, Myung-Ki Hwang, Yun-Jung Jung; Korean J Parasitol 2006;44(2):127-131.
Published online June 20, 2006; DOI: https://doi.org/10.3347/kjp.2006.44.2.127

Abstract
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The furcocercus cercariae of Neodiplostomum seoulense (Digenea: Neodiplostomidae) penetrate the skins of tadpoles and shed their tails. The speculated mechanism of this tail loss was physical efforts required to produce a vigorous zigzag motion during skin penetration; no other mechanism has been proposed. We examined the relationship between the host serum and cercarial tail loss. Cercariae of N. seoulense were collected from experimentally infected Segmentina hemisphaerula, and lots of 300 cercariae were cultured in medium 199 contained several types of sera. Cercarial tail degradation was induced in all media, but all the cercariae cultured except those cultured in media containing fetal bovine serum (FBS) died within 48 hr. After 72 hr cultivation in media containing FBS, cercarial tail degradation was induced in 67.0%; in continuous cultivation 13.3% of larvae survived for 7 days. Tail degradation did not occur in the absence of serum and when serum was heat inactivated at 56℃ for 30 min. The addition of 20 mM ethylenediaminetetraacetic acid (EDTA) blocked cercarial tail degradation completely. Moreover, the addition of 20 mM MgCl₂ restored tail degradation blocked by EDTA. These results suggest that the alternative complement pathway is related with the N. seoulense cercarial tail degradation induced by serum.

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Effects of specific monoclonal antibodies to dense granular proteins on the invasion of Toxoplasma gondii in vitro and in vivo: Dong Yeob Cha, In Kwan Song, Gye Sung Lee, Ok-Sun Hwang, Hyung-Jun Noh, Seung-Dong Yeo, Dae-Whan Shin, Young-Ha Lee; Korean J Parasitol 2001;39(3):233-240.
Published online September 30, 2001; DOI: https://doi.org/10.3347/kjp.2001.39.3.233

Abstract
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Although some reports have been published on the protective effect of antibodies to Toxoplasma gondii surface membrane proteins, few address the inhibitory activity of antibodies to dense granular proteins (GRA proteins). Therefore, we performed a series of experiments to evaluate the inhibitory effects of monoclonal antibodies (mAbs) to GRA proteins (GRA2, 28 kDa; GRA6, 32 kDa) and surface membrane protein (SAG1, 30 kDa) on the invasion of T. gondii tachyzoites. Passive immunization of mice with one of three mAbs following challenge with a lethal dose of tachyzoites significantly increased survival compared with results for mice treated with control ascites. The survival times of mice challenged with tachyzoites pretreated with anti-GRA6 or anti-SAG1 mAb were significantly increased. Mice that received tachyzoites pretreated with both mAb and complement had longer survival times than those that received tachyzoites pretreated with mAb alone. Invasion of tachyzoites into fibroblasts and macrophages was significantly inhibited in the anti-GRA2, anti-GRA6 or anti-SAG1 mAb pretreated group. Pretreatment with mAb and complement inhibited invasion of tachyzoites in both fibroblasts and macrophages. These results suggest that specific antibodies to dense-granule molecules may be useful for controlling infection with T. gondii.

Citations

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Frontiers in Immunology.2025;[Epub] CrossRef
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The Journal of Infectious Diseases.2021; 224(4): 705. CrossRef
Treatment of toxoplasmosis: Current options and future perspectives
Neda Konstantinovic, Hélène Guegan, Tijana Stäjner, Sorya Belaz, Florence Robert-Gangneux
Food and Waterborne Parasitology.2019; 15: e00036. CrossRef
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Marijo S. Roiko, Kaice LaFavers, Diane Leland, Gustavo Arrizabalaga
International Journal for Parasitology.2018; 48(3-4): 225. CrossRef
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Pedro Ferreirinha, Ricardo Fróis-Martins, Luzia Teixeira, António Rocha, Manuel Vilanova, Alexandra Correia
Vaccine.2018; 36(32): 4890. CrossRef
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Sherene Swee Yin Lim, Kek Heng Chua, Greta Nölke, Holger Spiegel, Wai Leong Goh, Sek Chuen Chow, Boon Pin Kee, Rainer Fischer, Stefan Schillberg, Rofina Yasmin Othman
PeerJ.2018; 6: e5780. CrossRef
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Xiao T. Ching, Mun Y. Fong, Yee L. Lau
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Recent Advances in Toxoplasma gondii Immunotherapeutics
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The Korean Journal of Parasitology.2014; 52(6): 581. CrossRef
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Yong-Feng Fu, Meng Feng, Kenji Ohnishi, Tamon Kimura, Johbu Itoh, Xun-Jia Cheng, Hiroshi Tachibana, J. H. Adams
Infection and Immunity.2011; 79(1): 512. CrossRef
GRA Proteins of Toxoplasma gondii: Maintenance of Host-Parasite Interactions across the Parasitophorous Vacuolar Membrane
Ho-Woo Nam
The Korean Journal of Parasitology.2009; 47(Suppl): S29. CrossRef
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E. Jongert, D. Verhelst, M. Abady, E. Petersen, N. Gargano
Vaccine.2008; 26(41): 5289. CrossRef
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Majid Golkar, Mohammad-Ali Shokrgozar, Sima Rafati, Karine Musset, Mehdi Assmar, Reza Sadaie, Marie-France Cesbron-Delauw, Corinne Mercier
Vaccine.2007; 25(21): 4301. CrossRef