Parasites, Hosts and Diseases

"crude antigen"

Original Articles

Serodiagnosis of Toxocariasis by ELISA Using Crude Antigen of Toxocara canis Larvae: Yan Jin, Chenghua Shen, Sun Huh, Woon-Mok Sohn, Min-Ho Choi, Sung-Tae Hong; Korean J Parasitol 2013;51(4):433-439.
Published online August 30, 2013; DOI: https://doi.org/10.3347/kjp.2013.51.4.433

Abstract
PDF

Toxocariasis is a worldwide zoonosis caused by larvae of ascarid nematodes of dogs or cats, Toxocara canis or T. cati. Diagnosis of human toxocariasis currently relies on serology that uses T. canis excretory-secretory antigen to detect specific IgG antibodies by ELISA. We investigated the serodiagnostic efficacy of ELISA using crude antigen of T. canis larvae (TCLA). Serum specimens of 64 clinically confirmed toxocariasis, 115 healthy controls, and 119 other tissue-invading helminthiases were screened by ELISA using TCLA. The ELISA using TCLA showed 92.2% (59/64 patient samples) sensitivity and 86.6% (103/119) specificity. Its positive diagnostic predictivity was 78.7% and negative predictivity was 97.8%. No serum of healthy controls reacted but that of anisakiasis (45.5%), gnathostomiasis (19.2%), clonorchiasis (15.8%), sparganosis (11.1%), and cysticercosis (6.3%) cross-reacted. Immunoblot analysis on TCLA recognized antigenic proteins of 28- and 30-kDa bands in their dominant protein quantity and strong blotting reactivity. The present results indicate that the ELISA using our TCLA antigen is acceptable by the sensitivity and specificity for serodiagnosis of human toxocariasis. ELISA with TCLA is recommended to make differential diagnosis for patients with any sign of organ infiltration and eosinophilia.

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Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA: Min-Ho Choi, Il Chan Park, Shunyu Li, Sung-Tae Hong; Korean J Parasitol 2003;41(1):35-39.
Published online March 20, 2003; DOI: https://doi.org/10.3347/kjp.2003.41.1.35

Abstract
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Although stool examination is the standard diagnostic method of clonorchiasis, serodiagnosis by ELISA using crude antigen is now widely used because of its convenience. However, ELISA diagnosis still suffers from cross-reactions, and therefore there is a need to improve the present conventional ELISA. The present study was undertaken to evaluate the diagnostic value of ELISA using excretory-secretory antigen (ESA) instead of crude antigen (CA) of Clonorchis sinensis. The diagnostic sensitivity of ELISA using excretory-secretory antigen was 92.5%, which was higher than that of ELISA using crude Clonorchis sinensis antigen (88.2%). In addition, the specificity of excretory-secretory antigen was found 93.1% while that of crude antigen was 87.8%. In summary, Clonorchis sinensis ESA was found to be a better serodiagnostic antigen than CA for ELISA.

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Multiple recombinant antigens of Clonorchis sinensis for serodiagnosis of human clonorchiasis
Shunyu Li, Jung Guk Shin, Pyo Yun Cho, Tae Im Kim, Sung-Tae Hong, Sung-Jong Hong
Parasitology Research.2011; 108(5): 1295. CrossRef
Performance of an Enzyme-Linked Immunosorbent Assay for Detection of Clonorchis sinensis Infestation in High- and Low-Risk Groups
Young Jin Kim, Sun Min Lee, Go Eun Choi, Sang Hyun Hwang, Hyung Hoi Kim, Eun Yup Lee, Chulhun L. Chang
Journal of Clinical Microbiology.2010; 48(7): 2365. CrossRef
Evaluation of excretory secretory and 10–30 kDa antigens of Taenia solium Cysticerci by EITB assay for the diagnosis of neurocysticercosis
S. R. V. ATLURI, P. SINGHI, N. KHANDELWAL, N. MALLA
Parasite Immunology.2009; 31(3): 151. CrossRef
Identification of a serodiagnostic antigen, legumain, by immunoproteomic analysis of excretory‐secretory products of Clonorchis sinensis adult worms
Jung‐Won Ju, Hyun‐Na Joo, Myoung‐Ro Lee, Shin‐Hyeong Cho, Hyeng‐Il Cheun, Jung‐Yeon Kim, Young‐Hee Lee, Kwang‐Jun Lee, Woon‐Mok Sohn, Dong‐Min Kim, Il‐Chul Kim, Byoung Chul Park, Tong‐Soo Kim
PROTEOMICS.2009; 9(11): 3066. CrossRef
Serodiagnostic applicability of recombinant antigens of Clonorchis sinensis expressed by wheat germ cell-free protein synthesis system
Chenghua Shen, Jong-Ae Lee, Sonia Refaat Ahmed Allam, Young Mee Bae, Eun-Taek Han, Satoru Takeo, Takafumi Tsuboi, Sung-Tae Hong, Min-Ho Choi
Diagnostic Microbiology and Infectious Disease.2009; 64(3): 334. CrossRef
Detection ofClonorchis sinensisin stool samples using real-time PCR
E.-M. Kim, J. J. Verweij, A. Jalili, L. van Lieshout, M.-H. Choi, Y. M. Bae, M. K. Lim, S.-T. Hong
Annals of Tropical Medicine & Parasitology.2009; 103(6): 513. CrossRef
Neurocysticercosis immunodiagnosis using Taenia solium cysticerci crude soluble extract, excretory secretory and lower molecular mass antigens in serum and urine samples of Indian children
Subba Rao V. Atluri, P. Singhi, N. Khandelwal, N. Malla
Acta Tropica.2009; 110(1): 22. CrossRef
Antigenic Properties of Cystatin-binding Cysteine Proteinases from Neodiplostomum seoulense
Tae-Yun Kim, Kye-Young Han, Eun-Hee Shin, Jong-Yil Chai
Journal of Parasitology.2008; 94(3): 654. CrossRef
Intraductal papillary neoplasm of the bile duct associated with Clonorchis sinensis infection
Kee-Taek Jang, Seung-Mo Hong, Kyu Taek Lee, Jong Gyun Lee, Seoung Ho Choi, Jin Seok Heo, Dong Wook Choi, Dongil Choi, Jae Hoon Lim
Virchows Archiv.2008; 453(6): 589. CrossRef
Factors in the resistance of rats to re-infection and super-infection by Clonorchis sinensis
Hongman Zhang, Byung-Suk Chung, Shunyu Li, Min-Ho Choi, Sung-Tae Hong
Parasitology Research.2008; 102(6): 1111. CrossRef
Changing Patterns of Serum and Bile Antibodies in Re-infected Rats with Clonorchis sinensis
Hongman Zhang, Byung-Suk Chung, Shunyu Li, Min-Ho Choi, Sung-Tae Hong
The Korean Journal of Parasitology.2008; 46(1): 17. CrossRef
Gallstones and Clonorchis sinensis infection: A hospital‐based case–control study in Korea
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Journal of Gastroenterology and Hepatology.2008;[Epub] CrossRef
Antigenic Properties of Cystatin-binding Cysteine Proteinases from Neodiplostomum seoulense
Tae-Yun Kim, Kye-Young Han, Eun-Hee Shin, Jong-Yil Chai
Journal of Parasitology.2008; 94(3): 654. CrossRef
Clonorchis sinensis: Expression, characterization, immunolocalization and serological reactivity of one excretory/secretory antigen-LPAP homologue
Fengyu Hu, Xinbing Yu, Changling Ma, Hongjuan Zhou, Zhenwen Zhou, Yanwen Li, Fangli Lu, Jin Xu, Zhongdao Wu, Xuchu Hu
Experimental Parasitology.2007; 117(2): 157. CrossRef
Tissue parasitic helminthiases are prevalent at Cheongjin, North Korea
Chenghua Shen, Shunyu Li, Shanzi Zheng, Min-Ho Choi, Young Mee Bae, Sung-Tae Hong
The Korean Journal of Parasitology.2007; 45(2): 139. CrossRef
Molecular characterization and serodiagnosis analysis of a novel lysophospholipase from Clonorchis sinensis
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Evaluation of a recombinant excretory secretory Haemonchus contortus protein for use in a diagnostic enzyme-linked immunosorbent assay
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Experimental Parasitology.2007; 115(3): 242. CrossRef
Clonorchis sinensis and Opisthorchis viverrini: Development of a mitochondrial-based multiplex PCR for their identification and discrimination
Thanh Hoa Le, Nguyen Van De, David Blair, Paiboon Sithithaworn, Donald P. McManus
Experimental Parasitology.2006; 112(2): 109. CrossRef
Cholangiocarcinoma and Clonorchis sinensis infection: A case–control study in Korea
Dongil Choi, Jae Hoon Lim, Kyu Taek Lee, Jong Kyun Lee, Seong Ho Choi, Jin Seok Heo, Kee-Taek Jang, Nam Yong Lee, Seonwoo Kim, Sung-Tae Hong
Journal of Hepatology.2006; 44(6): 1066. CrossRef
Fish-borne parasitic zoonoses: Status and issues
Jong-Yil Chai, K. Darwin Murrell, Alan J. Lymbery
International Journal for Parasitology.2005; 35(11-12): 1233. CrossRef
The identification of a Clonorchis sinensis gene encoding an antigenic egg protein
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Parasitology Research.2005; 95(3): 224. CrossRef
Organ-specific antigens of Clonorchis sinensis
Shunyu Li, Byung-Suk Chung, Min-Ho Choi, Sung-Tae Hong
The Korean Journal of Parasitology.2004; 42(4): 169. CrossRef
Evaluation of Clonorchis sinensis Recombinant 7-Kilodalton Antigen for Serodiagnosis of Clonorchiasis
Qin-Ping Zhao, Sung-Ung Moon, Hyeong-Woo Lee, Byoung-Kuk Na, Seung-Yull Cho, Yoon Kong, Ming-Sen Jiang, Ai-Hua Li, Tong-Soo Kim
Clinical and Vaccine Immunology.2004; 11(4): 814. CrossRef
Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini (Opisthorchidae, Trematoda)
Min-Ho Choi, Jin-Sook Ryu, Mejeong Lee, Shunyu Li, Byung-Suk Chung, Jong-Yil Chai, Paiboon Sithithaworn, Smarn Tesana, Sung-Tae Hong
The Korean Journal of Parasitology.2003; 41(3): 155. CrossRef