Cryptosporidium parvum is known as one of the most highly resistant parasites to gamma irradiation. To morphologically have an insight on the radioresistance of this parasite, ultrastructural changes in C. parvum sporozoites were observed after gamma irradiation using various doses (1, 5, 10, and 25 kGy) following a range of post-irradiation incubation times (10 kGy for 6, 12, 24, 48, 72, and 96 hr). The ultrastructures of C. parvum oocysts changed remarkably after a 10-kGy irradiation. Nuclear membrane changes and degranulation of dense granules were observed with high doses over 10 kGy, and morphological changes in micronemes and rhoptries were observed with very high doses over 25 kGy. Oocyst walls were not affected by irradiation, whereas the internal structures of sporozoites degenerated completely 96 hr post-irradiation using a dose of 10 kGy. From this study, morphological evidence of radioresistance of C. parvum has been supplemented.
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Cryptosporidium parvum is a well-known waterborne and opportunistic intracellular protozoan parasite that causes diarrheal illness. In this study, we quantitatively investigated reduction of the infectivity of C. parvum after gamma irradiation and repair of the infectivity during incubation time after irradiation. C. parvum oocysts were subjected to gamma irradiation at various doses (1, 5, 10, and 25 kGy), and the in vitro infectivity was measured by real-time PCR every day up to 7 days after irradiation. The in vitro infectivity of C. parvum on human ileocecal adenocarcinoma cells (HCT-8) was effectively reduced (> 2 log10) by irradiation at 10 kGy or more. However, in the experiment to find out repair of the infectivity, recovery was not noted until day 7 post-incubation.
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Ultrastructural Changes inCryptosporidium parvumOocysts by Gamma Irradiation Migyo Joung, Sejeong Yun, Mijoung Joung, Woo-Yoon Park, Jae-Ran Yu The Korean Journal of Parasitology.2011; 49(1): 25. CrossRef
Effect of Infrared Irradiation of Oocysts on the Pathology of Experimental Cryptosporidiosis in Apparently Normal and Immunosuppressed Mice Hafidh I. Al-Sadi, Saevan S. Al-Mahm Research Journal of Biological Sciences.2010; 5(2): 177. CrossRef
Cryptosporidiosis Jae-Ran Yu Hanyang Medical Reviews.2010; 30(3): 187. CrossRef
Rejoining of gamma-ray-induced DNA damage in Cryptosporidium parvum measured by the comet assay Soo-Ung Lee, Mikyo Joung, Taekyoung Nam, Woo-Yoon Park, Jae-Ran Yu Experimental Parasitology.2010; 125(3): 230. CrossRef
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