Parasites, Hosts and Diseases

"granulocyte"

Original Articles

Molecular Detection and Phylogenetic Analysis of Anaplasma phagocytophilum in Horses in Korea: Min-Goo Seo, In-Ohk Ouh, Eunsang Choi, Oh-Deog Kwon, Dongmi Kwak; Korean J Parasitol 2018;56(6):559-565.
Published online December 31, 2018; DOI: https://doi.org/10.3347/kjp.2018.56.6.559

Abstract
PDF

The identification and characterization of pathogenic and zoonotic tick-borne diseases like granulocytic anaplasmosis are essential for developing effective control programs. The differential diagnosis of pathogenic Anaplasma phagocytophilum and non-pathogenic A. phagocytophilum-like Anaplasma spp. is important for implementing effective treatment from control programs. The
objective
of the present study was to investigate the prevalence of Anaplasma spp. in horses in Korea by nucleotide sequencing and restriction enzyme fragment length polymorphism assay. Of the 627 horses included in the study, only 1 (0.2%) was infected with A. phagocytophilum. Co-infection with A. phagocytophilum- like Anaplasma spp. was not detected in the study. The 16S rRNA sequence of A. phagocytophilum was similar (99.5- 100%) to A. phagocytophilum 16S rRNA isolated from horses in other countries. PCR adapted to amplify A. phagocytophilum groEL and msp2 genes failed to generate amplicons, suggesting genetic diversity in these genes. This study is the first molecular detection of A. phagocytophilum in horses in Korea. Human granulocytic anaplasmosis and animal infection of A. phagocytophilum have been reported in Korea recently. Because of vector tick distribution, global warming, and the increase of the horse industry, horses should be considered as a potential reservoir for A. phagocytophilum, and cross infectivity should be evaluated even though a low prevalence of infection was detected in this study. Furthermore, continuous surveillance and effective control measures for A. phagocytophilum should be established to prevent disease distribution and possible transmission to humans.

Citations

Citations to this article as recorded by

PRELIMINARY DATA ON MOLECULAR TESTING FOR ANAPLASMA PHAGOCYTOPHILUM INFECTION IN CATTLE AND HORSES, ROMANIA
Emanuel MITREA, Andreea Monica BOGDAN , Anca Maria ZISOPOL , Ioan Liviu MITREA , Mariana IONIȚĂ
Scientific Papers Journal VETERINARY SERIES.2024; 67(1): 24. CrossRef
Worldwide meta-analysis on Anaplasma phagocytophilum infections in animal reservoirs: Prevalence, distribution and reservoir diversity
Solomon Ngutor Karshima, Musa Isiyaku Ahmed, Kaltume Mamman Mohammed, Victoria Adamu Pam, Habiba Momoh-Abdullateef, Bulus Peter Gwimi
Veterinary Parasitology: Regional Studies and Reports.2023; 38: 100830. CrossRef
Detection and Genotypic Analysis of Anaplasma bovis and A. phagocytophilum in Horse Blood and Lung Tissue
Min-Goo Seo, In-Ohk Ouh, Dongmi Kwak
International Journal of Molecular Sciences.2023; 24(4): 3239. CrossRef
Tick Populations and Molecular Analysis of Anaplasma Species in Ticks from the Republic of Korea
Min-Goo Seo, Haeseung Lee, Badriah Alkathiri, KyuSung Ahn, Seung-Hun Lee, SungShik Shin, Seulgi Bae, Kyoo-Tae Kim, Min Jang, Sang-Kwon Lee, Yun Sang Cho, Kyung-Yeon Eo, Oh-Deog Kwon, Dongmi Kwak
Microorganisms.2023; 11(4): 820. CrossRef
The first study on the seroprevalence of Anaplasma spp. in small ruminants and assessment of associated risk factors in North Egypt
Abdelfattah Selim, Kotb A. Attia, Roua A. Alsubki, Fatima Albohairy, Itoh Kimiko, Mourad Ben Said
Veterinary World.2022; : 1221. CrossRef
Molecular epidemiology of anaplasmosis in small ruminants along a human-livestock-wildlife interface in Uganda
Keneth Iceland Kasozi, Susan Christina Welburn, Gaber El-Saber Batiha, Najat Marraiki, David Paul Nalumenya, Monica Namayanja, Kevin Matama, Kelly Katenta Zalwango, Wycliff Matovu, Gerald Zirintunda, Justine Ekou, Stellamaris Kembabazi, Claire Mack Mugasa
Heliyon.2021; 7(1): e05688. CrossRef
Synergic Effect of Adsorption and Biodegradation by Microsphere Immobilizing Bacillus velezensis for Enhanced Removal Organics in Slaughter Wastewater
Jing Deng, Qijue Chen, Boyong Hu, Wen Li, Mingxi Jia, Yi Shi, Shouyao Xiong, Jie Bai, Huaqun Yin
Processes.2021; 9(7): 1145. CrossRef
Molecular Detection and Phylogenetic Analysis of Tick-Borne Pathogens in Ticks Collected from Horses in the Republic of Korea
Hyun-Ji Seo, A-Tai Truong, Keun-Ho Kim, Ji-Yeon Lim, Subin Min, Heung-Chul Kim, Mi-Sun Yoo, Soon-Seek Yoon, Terry A. Klein, Yun Sang Cho
Pathogens.2021; 10(9): 1069. CrossRef
First Molecular Detection and Phylogenetic Analysis of Anaplasma phagocytophilum in Horses in Muş Province of Turkey
Bekir OĞUZ
Kocaeli Üniversitesi Sağlık Bilimleri Dergisi.2021; 7(3): 312. CrossRef
Duplex TaqMan real-time PCR assay for simultaneous detection and quantification of Anaplasma capra and Anaplasma phagocytophilum infection
Jinxing Song, Shanshan Zhao, Yueqin Li, Haiyan Wang, Liwei Zhang, Jun Wang, Changshen Ning, Yongshuai Peng
Molecular and Cellular Probes.2020; 49: 101487. CrossRef
Anaplasma bovis infection in a horse: First clinical report and molecular analysis
Min-Goo Seo, Oh-Deog Kwon, Dongmi Kwak
Veterinary Microbiology.2019; 233: 47. CrossRef
Seroprevalence of Anaplasma spp. among sheep and goats in Charsadda District, Pakistan
Adil Khan, Nasreen, Robert D. Mitchell, Sadaf Niaz, Sultan Ayaz, Irfan Khattak, Huma Naeem, Adalberto A. Pérez de León, Muhammad Arfan Zaman
Small Ruminant Research.2019; 176: 5. CrossRef

8,549 View
153 Download
11 Web of Science
Crossref

Sequential analysis of cell differentials and IFN-γ production of splenocytes from mice infected with Toxoplasma gondii: Young-Ha Lee, Dae-Whan Shin, Lloyd H. Kasper; Korean J Parasitol 2000;38(2):85-90.
Published online June 30, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.2.85

Abstract
PDF

To assess the relationship between the changes of cellular components and the production of Th1 cytokine in the immune tissue, inbred C57BL/6 mice were orally infected with 40 cysts of 76K strain of Toxoplasma gondii. The sequential change of cell differentials and IFN-γ production of splenocytes were analyzed by Diff-Quik stain and RT-PCR. There were no significant proportional changes of cellular components of splenocytes until day 4 postinfection (PI) as compared to those of day 0, and the relative percentage of macrophages and neutrophils/eosinophils increased significantly (p<0.01) thereafter. The expression of IFN-γ mRNA of CD3^- cells was observed from day 1 PI at a low level. However, IFN-γ production of CD3⁺ cells increased significantly from day 4 PI (p<0.01) which progressively increased thereafter. These findings provide the relative percentages of granulocytes and macrophages were increased in conjunction with increase of total number of splenocytes after oral infection with T. gondii in the susceptible murine hosts, and lymphocytes were the major cellular components and the important source of IFN-γ.

Citations

Citations to this article as recorded by

Oral Tolerization with Cardiac Myosin Peptide (614–629) Ameliorates Experimental Autoimmune Myocarditis: Role of Stat 6 Genes in BALB/CJ Mice
Patricia A. Gonnella, Pedro J. Del Nido, Francis X. McGowan
Journal of Clinical Immunology.2009; 29(4): 434. CrossRef
T cell phenotype and intracellular IFN-γ production in peritoneal exudate cells and gut intraepithelial lymphocytes during acute Toxoplasma gondii infection in mice
Young-Ha Lee, Dae-Whan Shin
The Korean Journal of Parasitology.2002; 40(3): 119. CrossRef

8,175 View
82 Download
Crossref

Brief Communication

Excretory-secretory product of Paragonimus westermani newly excysted metacercariae inhibits superoxide production of granulocytes stimulated with IgG: Myeong Heon Shin; Korean J Parasitol 2000;38(2):103-106.
Published online June 30, 2000; DOI: https://doi.org/10.3347/kjp.2000.38.2.103

Abstract
PDF

It is well known that the cysteine proteases in excretory-secretory product (ESP) of Paragonimus westermani newly excysted metacercariae (PwNEM) are capable of degrading IgG in vitro. Recent evidence suggests that the IgG-coated surface, such as found on parasites, is one of the most effective physiologic stimuli for granulocyte activation. Therefore, this study was designed to investigate the effect of excretory-secretory product (ESP) of PwNEM on superoxide production of granulocytes stimulated with IgG. The 96-well plates were coated with human IgG (0, 10, 30, 100 ?g/ml) in the absence or presence of ESP. When granulocytes were incubated in the wells coated with human IgG in the presence of ESP, the level of superoxide production of granulocytes was reduced to about 90% when compared to the cells incubated in the wells coated with IgG alone. This inhibitory effect of the ESP on IgG-induced superoxide production of granulocytes was concentration-dependent. These results suggest that ESP secreted by PwNEM may be important in the control of effector functions of granulocytes stimulated with IgG in human paragonimiasis.

Citations

Citations to this article as recorded by

Excretory-secretory product of third-stage Gnathostoma spinigerum larvae induces apoptosis in human peripheral blood mononuclear cells
Nareerat Viseshakul, Wilanee Dechkhajorn, Surachet Benjathummarak, Supaporn Nuamtanong, Yaowapa Maneerat
Parasitology Research.2017; 116(10): 2783. CrossRef
Superoxide Anion Production by Human Neutrophils Activated by Trichomonas vaginalis
Hyun-Ouk Song, Jae-Sook Ryu
The Korean Journal of Parasitology.2013; 51(4): 479. CrossRef
Excretory-Secretory Products Produced by Paragonimus westermani Differentially Regulate the Nitric Oxide Production and Viability of Microglial Cells
Youngnam Jin, Jae-Chul Lee, In Young Choi, Eun A. Kim, Myeong Heon Shin, Won-Ki Kim
International Archives of Allergy and Immunology.2006; 139(1): 16. CrossRef