Parasites, Hosts and Diseases

"microsatellite"

Original Articles

Genetic Diversity of Plasmodium vivax in Clinical Isolates from Southern Thailand using PvMSP1, PvMSP3 (PvMSP3α, PvMSP3β) Genes and Eight Microsatellite Markers: Supinya Thanapongpichat, Thunchanok Khammanee, Nongyao Sawangjaroen, Hansuk Buncherd, Aung Win Tun; Korean J Parasitol 2019;57(5):469-479.
Published online October 31, 2019; DOI: https://doi.org/10.3347/kjp.2019.57.5.469

Abstract
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Plasmodium vivax is usually considered morbidity in endemic areas of Asia, Central and South America, and some part of Africa. In Thailand, previous studies indicated the genetic diversity of P. vivax in malaria-endemic regions such as the western part of Thailand bordering with Myanmar. The
objective
of the study is to investigate the genetic diversity of P. vivax circulating in Southern Thailand by using 3 antigenic markers and 8 microsatellite markers. Dried blood spots were collected from Chumphon, Phang Nga, Ranong and, Surat Thani provinces of Thailand. By PCR, 3 distinct sizes of PvMSP3α, 2 sizes of PvMSP3β and 2 sizes of PvMSP1 F2 were detected based on the length of PCR products, respectively. PCR/RFLP analyses of these antigen genes revealed high levels of genetic diversity. The genotyping of 8 microsatellite loci showed high genetic diversity as indicated by high alleles per locus and high expected heterozygosity (H_E). The genotyping markers also showed multiple-clones of infection. Mixed genotypes were detected in 4.8% of PvMSP3α, 29.1% in PvMSP3β and 55.3% of microsatellite markers. These results showed that there was high genetic diversity of P. vivax isolated from Southern Thailand, indicating that the genetic diversity of P. vivax in this region was comparable to those observed other areas of Thailand.

Citations

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Genetic diversity of Plasmodium vivax and Plasmodium falciparum field isolates from Honduras in the malaria elimination phase
Alejandro Zamora, Alejandra Pinto, Denis Escobar, Hugo O. Valdivia, Lesly Chaver, Gloria Ardón, Erick Carranza, Gustavo Fontecha
Current Research in Parasitology & Vector-Borne Diseases.2025; 7: 100230. CrossRef
Genetic Structure of Introduced Plasmodium vivax Malaria Isolates in Greece, 2015–2019
Ioanna Spiliopoulou, Danai Pervanidou, Nikolaos Tegos, Maria Tseroni, Agoritsa Baka, Annita Vakali, Chrisovaladou-Niki Kefaloudi, Vasilios Papavasilopoulos, Anastasia Mpimpa, Eleni Patsoula
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Asymptomatic Malaria Reservoirs in Honduras: A Challenge for Elimination
Sharon Banegas, Denis Escobar, Alejandra Pinto, Marcela Moncada, Gabriela Matamoros, Hugo O. Valdivia, Allan Reyes, Gustavo Fontecha
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Distinct Allelic Diversity of Plasmodium vivax Merozoite Surface Protein 3-Alpha (PvMSP-3α) Gene in Thailand Using PCR-RFLP
Kanyanan Kritsiriwuthinan, Warunee Ngrenngarmlert, Rapatbhorn Patrapuvich, Supaksajee Phuagthong, Kantima Choosang, Jianbing Mu
Journal of Tropical Medicine.2023; 2023: 1. CrossRef
Genetic Diversity of Plasmodium vivax Field Isolates from the Thai–Myanmar Border during the Period of 2006–2016
Abdifatah Abdullahi Jalei, Wanna Chaijaroenkul, Kesara Na-Bangchang
Tropical Medicine and Infectious Disease.2023; 8(4): 210. CrossRef
Genetic diversity and molecular evolution of Plasmodium vivax Duffy Binding Protein and Merozoite Surface Protein-1 in northwestern Thailand
Parsakorn Tapaopong, Gustavo da Silva, Sittinont Chainarin, Chayanut Suansomjit, Khajohnpong Manopwisedjaroen, Liwang Cui, Cristian Koepfli, Jetsumon Sattabongkot, Wang Nguitragool
Infection, Genetics and Evolution.2023; 113: 105467. CrossRef
Genetic Diversity of Plasmodium vivax Merozoite Surface Protein-3 Alpha and Beta from Diverse Geographic Areas of Thailand
Jiraporn Kuesap, Kanchana Rungsihirunrat, Wanna Chaijaroenkul, Mathirut Mungthin
Japanese Journal of Infectious Diseases.2022; 75(3): 241. CrossRef
Prevalence of Glucose-6-Phosphate Dehydrogenase (G6PD) Deficiency among Malaria Patients in Southern Thailand: 8 Years Retrospective Study
Thunchanok Khammanee, Nongyao Sawangjaroen, Hansuk Buncherd, Aung Win Tun, Supinya Thanapongpichat
The Korean Journal of Parasitology.2022; 60(1): 15. CrossRef
PvMSP-3α and PvMSP-3β genotyping reveals higher genetic diversity in Plasmodium vivax parasites from migrant workers than residents at the China-Myanmar border
Xiaosong Li, Yao Bai, Yanrui Wu, Weilin Zeng, Zheng Xiang, Hui Zhao, Wei Zhao, Xi Chen, Mengxi Duan, Xun Wang, Wenya Zhu, Kemin Sun, Yiman Wu, Yanmei Zhang, Yucheng Qin, Benjamin M. Rosenthal, Liwang Cui, Zhaoqing Yang
Infection, Genetics and Evolution.2022; 106: 105387. CrossRef
Genetic characterization of Plasmodium vivax isolates from Pakistan using circumsporozoite protein (pvcsp) and merozoite surface protein-1 (pvmsp-1) genes as genetic markers
Zainab Bibi, Anam Fatima, Rehana Rani, Ayesha Maqbool, Samea Khan, Shumaila Naz, Shahid Waseem
Malaria Journal.2021;[Epub] CrossRef

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Population Genetics of Plasmodium vivax in Four High Malaria Endemic Areas in Thailand: Kanungnit Congpuong, Ratawan Ubalee; Korean J Parasitol 2017;55(5):465-472.
Published online October 31, 2017; DOI: https://doi.org/10.3347/kjp.2017.55.5.465

Abstract
PDF

Recent trends of malaria in Thailand illustrate an increasing proportion of Plasmodium vivax, indicating the importance of P. vivax as a major causative agent of malaria. P. vivax malaria is usually considered a benign disease so the knowledge of this parasite has been limited, especially the genetic diversity and genetic structure of isolates from different endemic areas. The aim of this study was to examine the population genetics and structure of P. vivax isolates from 4 provinces with different malaria endemic settings in Thailand using 6 microsatellite markers. Total 234 blood samples from P. vivax mono-infected patients were collected. Strong genetic diversity was observed across all study sites; the expected heterozygosity values ranged from 0.5871 to 0.9033. Genetic variability in this study divided P. vivax population into 3 clusters; first was P. vivax isolates from Mae Hong Son and Kanchanaburi Provinces located on the western part of Thailand; second, Yala isolates from the south; and third, Chanthaburi isolates from the east. P. vivax isolates from patients having parasite clearance time (PCT) longer than 24 hr after the first dose of chloroquine treatment had higher diversity when compared with those having PCT within 24 hr. This study revealed a clear evidence of different population structure of P. vivax from different malaria endemic areas of Thailand. The findings provide beneficial information to malaria control programme as it is a useful tool to track the source of infections and current malaria control efforts.

Citations

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Low Genetic Diversity of Plasmodium vivax Circumsporozoite Surface Protein in Clinical Isolates from Southern Thailand
Tachin Khulmanee, Thanyapit Thita, Kanyanan Kritsiriwutinan, Usa Boonyuen, Aminoh Saai, Kanjana Inkabjan, Rimi Chakrabarti, Pradipsinh K. Rathod, Srivicha Krudsood, Mathirut Mungthin, Rapatbhorn Patrapuvich
Tropical Medicine and Infectious Disease.2024; 9(5): 94. CrossRef
Plasmodium vivax populations in the western Greater Mekong Subregion evaluated using a genetic barcode
Yubing Hu, Yuling Li, Awtum M. Brashear, Weilin Zeng, Zifang Wu, Lin Wang, Haichao Wei, Myat Thu Soe, Pyae Linn Aung, Jetsumon Sattabongkot, Myat Phone Kyaw, Zhaoqing Yang, Yan Zhao, Liwang Cui, Yaming Cao, Karin Kirchgatter
PLOS Neglected Tropical Diseases.2024; 18(7): e0012299. CrossRef
Extensive genetic diversity in Plasmodium vivax from Sudan and its genetic relationships with other geographical isolates
Musab M. Ali Albsheer, Alfred Hubbard, Cheikh Cambel Dieng, Eyoab Iyasu Gebremeskel, Safaa Ahmed, Virginie Rougeron, Muntaser E. Ibrahim, Eugenia Lo, Muzamil M. Abdel Hamid
Infection, Genetics and Evolution.2024; 123: 105643. CrossRef
Comparison of Vector Trapping Methods for Outdoor Biting Malaria Vector Surveillance in Thailand and Vietnam
Ratchadawan Ngoenklan, Tran Thanh Duong, Vu Duc Chinh, Nguyen Quang Thieu, Jeffrey Hii, Michael J Bangs, Boonserm Aum-Aung, Wannapa Suwonkerd, Theeraphap Chareonviriyaphap, Nobuko Tuno
Journal of Medical Entomology.2022; 59(6): 2139. CrossRef
Dynamics of Plasmodium vivax populations in border areas of the Greater Mekong sub-region during malaria elimination
Yuling Li, Yubing Hu, Yan Zhao, Qinghui Wang, Huguette Gaelle Ngassa Mbenda, Veerayuth Kittichai, Saranath Lawpoolsri, Jetsumon Sattabongkot, Lynette Menezes, Xiaoming Liu, Liwang Cui, Yaming Cao
Malaria Journal.2020;[Epub] CrossRef

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Brief Communication

High Genetic Variability of Schistosoma haematobium in Mali and Nigeria: Charles Ezeh, Mingbo Yin, Hongyan Li, Ting Zhang, Bin Xu, Moussa Sacko, Zheng Feng, Wei Hu; Korean J Parasitol 2015;53(1):129-134.
Published online February 27, 2015; DOI: https://doi.org/10.3347/kjp.2015.53.1.129

Abstract
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Schistosoma haematobium is one of the most prevalent parasitic flatworms, infecting over 112 million people in Africa. However, little is known about the genetic diversity of natural S. haematobium populations from the human host because of the inaccessible location of adult worms in the host. We used 4 microsatellite loci to genotype individually pooled S. haematobium eggs directly from each patient sampled at 4 endemic locations in Africa. We found that the average allele number of individuals from Mali was significantly higher than that from Nigeria. In addition, no significant difference in allelic composition was detected among the populations within Nigeria; however, the allelic composition was significantly different between Mali and Nigeria populations. This study demonstrated a high level of genetic variability of S. haematobium in the populations from Mali and Nigeria, the 2 major African endemic countries, suggesting that geographical population differentiation may occur in the regions.

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Geographical Influence on Morphometric Variability of Genetically “Pure” Schistosoma haematobium Eggs from Sub-Saharan Migrants in Spain
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Tropical Medicine and Infectious Disease.2023; 8(3): 144. CrossRef
Genetic Diversity of Schistosoma haematobium in Sub-Saharan Africa: A Systematic Review
Rabecca Tembo, Panji Nkhoma, Mildred Zulu, Florence Mwaba, John Yabe, Hikabasa Halwiindi, Moses Kamwela, King S Nalubamba, Chummy S Sikasunge, Andrew M Phri
University of Zambia Journal of Agricultural and Biomedical Sciences.2022;[Epub] CrossRef
Membrane Technology for Rapid Point-of-Care Diagnostics for Parasitic Neglected Tropical Diseases
Madeleine J. Rogers, Donald P. McManus, Stephen Muhi, Catherine A. Gordon
Clinical Microbiology Reviews.2021;[Epub] CrossRef
Detection of Schistosoma DNA in genital specimens and urine: A comparison between five female African study populations originating from S. haematobium and/or S. mansoni endemic areas
P. Pillay, J.A. Downs, J.M. Changalucha, E.A.T. Brienen, C.E. Ramarokoto, P.D.C. Leutscher, B.J. Vennervald, M. Taylor, E.F. Kjetland, L. Van Lieshout
Acta Tropica.2020; 204: 105363. CrossRef
RETRACTED: Diversity and Compatibility of Human Schistosomes and Their Intermediate Snail Hosts
Benjamin Sanogo, Dongjuan Yuan, Xin Zeng, Yanhua Zhang, Zhongdao Wu
Trends in Parasitology.2018; 34(6): 493. CrossRef
Geographic strain differentiation of Schistosoma japonicum in the Philippines using microsatellite markers
Kharleezelle J. Moendeg, Jose Ma M. Angeles, Ryo Nakao, Lydia R. Leonardo, Ian Kendrich C. Fontanilla, Yasuyuki Goto, Masashi Kirinoki, Elena A. Villacorte, Pilarita T. Rivera, Noboru Inoue, Yuichi Chigusa, Shin-ichiro Kawazu, Aaron R. Jex
PLOS Neglected Tropical Diseases.2017; 11(7): e0005749. CrossRef
Genetic Diversity of <i>Schistosoma haematobium</i> Eggs Isolated from Human Urine in Sudan
Juan-Hua Quan, In-Wook Choi, Hassan Ahmed Hassan Ahmed Ismail, Abdoelohab Saed Mohamed, Hoo-Gn Jeong, Jin-Su Lee, Sung-Tae Hong, Tai-Soon Yong, Guang-Ho Cha, Young-Ha Lee
The Korean Journal of Parasitology.2015; 53(3): 271. CrossRef

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Mini Review

Large-scale Genotyping and Genetic Mapping in Plasmodium Parasites: Xin-zhuan Su, Hongying Jiang, Ming Yi, Jianbing Mu, Robert M. Stephens; Korean J Parasitol 2009;47(2):83-91.
Published online May 26, 2009; DOI: https://doi.org/10.3347/kjp.2009.47.2.83

Abstract
PDF

The completion of many malaria parasite genomes provides great opportunities for genomewide characterization of gene expression and high-throughput genotyping. Substantial progress in malaria genomics and genotyping has been made recently, particularly the development of various microarray platforms for large-scale characterization of the Plasmodium falciparum genome. Microarray has been used for gene expression analysis, detection of single nucleotide polymorphism (SNP) and copy number variation (CNV), characterization of chromatin modifications, and other applications. Here we discuss some recent advances in genetic mapping and genomic studies of malaria parasites, focusing on the use of high-throughput arrays for the detection of SNP and CNV in the P. falciparum genome. Strategies for genetic mapping of malaria traits are also discussed.

Citations

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Experimental evidence for hybridization of closely related lineages in Plasmodium relictum
Vaidas Palinauskas, Rasa Bernotienė, Rita Žiegytė, Staffan Bensch, Gediminas Valkiūnas
Molecular and Biochemical Parasitology.2017; 217: 1. CrossRef
Genetic diversity of Plasmodium falciparum in human malaria cases in Mali
Cécile Nabet, Safiatou Doumbo, Fakhri Jeddi, Salimata Konaté, Tommaso Manciulli, Bakary Fofana, Coralie L’Ollivier, Aminata Camara, Sandra Moore, Stéphane Ranque, Mahamadou A. Théra, Ogobara K. Doumbo, Renaud Piarroux
Malaria Journal.2016;[Epub] CrossRef
Complexity of Infection and Genetic Diversity in Cambodian Plasmodium vivax
Lindsey R. Friedrich, Jean Popovici, Saorin Kim, Lek Dysoley, Peter A. Zimmerman, Didier Menard, David Serre, Jane M. Carlton
PLOS Neglected Tropical Diseases.2016; 10(3): e0004526. CrossRef
Genetic structure of Plasmodium vivax using the merozoite surface protein 1 icb5-6 fragment reveals new hybrid haplotypes in southern Mexico
René Cerritos, Lilia González-Cerón, José A Nettel, Ana Wegier
Malaria Journal.2014;[Epub] CrossRef
Infectious diseases detection by microarray: An overview of clinical relevant infections
Sara Elisa Herrera-Rodriguez, Darwin Elizondo-Quiroga, Ikuri Alvarez-Maya
Journal of Biomedical Science and Engineering.2013; 06(10): 1006. CrossRef