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"microscopic detection"

Original Article
A novel kit for enrichment of fecal helminth eggs
Eunsol Lee, Seon-Ok Back, Young-Ju Lee, Jung-Won Ju, Hee-Il Lee, Myoung-Ro Lee
Parasites Hosts Dis 2024;62(3):323-329.
Published online August 26, 2024
DOI: https://doi.org/10.3347/PHD.24021
We developed a new concentration kit, called the ParaEgg (PE), for easy detection trematode eggs from fecal samples in endemic areas of clonorchiasis and metagonimiasis in Korea. To create a standard of detection efficiency, 120 fecal samples were examined using the water–ether concentration method (WECM). The PE kit and Mini ParaSep (PS) kit were used to compare the detection sensitivity of 100 egg-positive and 20 egg-negative samples in WECM. Additionally, stool samples, which were intentionally spiked with 10, 20, and 30 Clonorchis sinensis eggs, were evaluated to assess the sensitivity in lowinfection cases. The PE and PS kits showed detection rates of 100% and 92%, respectively, from 100 egg-positive samples in WECM. Meanwhile, eggs were detected in 3 (PE) and 2 (PS) out of 20 egg-negative samples in WECM. The PE kit detected the highest number of eggs per gram of feces (727 on average), followed by the WECM (524) and PS kit (432). In fecal samples that were intentionally spiked with 10, 20, and 30 C. sinensis eggs, PE only detected eggs 2 out of 5 samples in 10 eggs spiked (40%), and the detection rates were 80% and 100%, respectively. The PE kit enabled a more accurate identification of trematode eggs because of the clearance of small fecal debris in the microscopic field. In conclusion, the PE kit is obviously helpful to detect and identify trematode eggs in stool examinations especially in endemic areas of clonorchiasis and metagonimiasis.

Citations

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  • 2024년 장내기생충 유행지역 감염 조사

    Public Health Weekly Report.2025; 18(19): 707.     CrossRef
  • Diagnostic performance evaluation of ParaEgg for identifying intestinal helminthiasis: A comparative study with conventional copromicroscopy
    Tilak Chandra Nath, Heeil Lee, Md. Mahamudul Hasan, Tanmoy Roy Rudro, Dipta Das, Md. Taufiqur Rahman, Nandiny Saha Roy, Pritha Parial, Proloy Chakraborty Tusher, Tarek Siddiki
    Parasite Epidemiology and Control.2025; 30: e00449.     CrossRef
  • Detection of intestinal parasites in leopard cat fecal samples using shotgun metagenomics
    Jun Ho Choi, Singeun Oh, Myung-hee Yi, Dongjun Kang, Du-Yeol Choi, Xavier Chavarria, Arwa Shatta, Yoon Hee Cho, Seongjun Choe, Seung-Hun Lee, Ju Yeong Kim
    Parasites, Hosts and Diseases.2025; 63(4): 349.     CrossRef
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