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Research Progress on Leptotrombidium deliense
Yan Lv, Xian-Guo Guo, Dao-Chao Jin
Korean J Parasitol 2018;56(4):313-324.
Published online August 31, 2018
DOI: https://doi.org/10.3347/kjp.2018.56.4.313
This article reviews Leptotrombidium deliense, including its discovery and nomenclature, morphological features and identification, life cycle, ecology, relationship with diseases, chromosomes and artificial cultivation. The first record of L. deliense was early in 1922 by Walch. Under the genus Leptotrombidium, there are many sibling species similar to L. deliense, which makes it difficult to differentiate L. deliense from another sibling chigger mites, for example, L. rubellum. The life cycle of the mite (L. deliense) includes 7 stages: egg, deutovum (or prelarva), larva, nymphochrysalis, nymph, imagochrysalis and adult. The mite has a wide geographical distribution with low host specificity, and it often appears in different regions and habitats and on many species of hosts. As a vector species of chigger mite, L. deliense is of great importance in transmitting scrub typhus (tsutsugamushi disease) in many parts of the world, especially in tropical regions of Southeast Asia. The seasonal fluctuation of the mite population varies in different geographical regions. The mite has been successfully cultured in the laboratory, facilitating research on its chromosomes, biochemistry and molecular biology.

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Original Articles

Geographical Distribution and Relative Abundance of Vectors of Scrub Typhus in the Republic of Korea
In Yong Lee, Heung Chul Kim, Young-Sun Lee, Jang Hoon Seo, Jae Won Lim, Tae Soon Yong, Terry A. Klein, Won Ja Lee
Korean J Parasitol 2009;47(4):381-386.
Published online December 1, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.4.381

A survey to determine the geographical distribution and relative abundance of potential vectors of scrub typhus was conducted from October to November 2006 at 13 localities throughout the Republic of Korea. Apodemus agrarius accounted for 97.6% (80/82) of all rodents, while only 2 Myodes regulus (2/82) were collected. A total of 10,860 chiggers were collected from A. agrarius belonging to 4 genera and 8 species, while only Walchia fragilis (40) was collected from Myodes regulus. Leptotrombidium pallidum (8,137; 74.9%), a vector of scrub typhus, was the predominant species collected from A. agrarius followed by Leptotrombidium scutellare (2,057, 18.9%), Leptotrombidium palpale (279; 2.7%), Leptotrombidium orientale (232; 2.1%), and Leptotrombidium zetum (79; 0.7%), Neotrombicula tamiyai (58; 0.5%), Euschoengastica koreaensis (16; 0.1%), and Cheladonta ikaoensis (2; < 0.1%). L. pallidum was the predominant chigger collected at collection sites in Gangwon (100%), Gyeonggi (87.2%), Chungnam (100%), Chungbuk (100%), Jeonbuk (73.9%), Jeonnam (77.0%), and Gyeongbuk (66.1%) provinces, whereas L. scutellare was the predominant chigger collected in Gyeongnam province (77.9%) and Jeju Island (62.3%). Data suggest a correlation between chigger population abundance and human cases of scrub typhus in Korea.

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This experiment was undertaken to screen the acaricidal effects of herb essential oils (pennyroyal, ylang ylang, citronella, lemon grass, tea tree, and rosemary) at different doses (0.1, 0.05, 0.025, 0.0125, and 0.00625 ?l/cm2) and exposure times (5, 10, 20, 20, 30 and 60 min) on house dust mites Dermatophgoides farinae and D. pteronyssinus. The most effective acaricidal components of pennyroyal (Mentha pulegium) were analyzed using a gas chromatography-mass spectrometer (GC-MS). Of these essential oils, the most effective was pennyroyal, which is composed essentially of pulegone (> 99%), at a dose of 0.025 ?l/cm2 which at an exposure time of 5 min killed more than 98% of house dust mites. In the pennyroyal fumigation test, the closed method was more effective than the open method and maximum acaricidal effect was 100% at 0.025 ?l/cm2, 60 min. The results show that herb essential oils, in particular, pennyroyal was proved to have potent acaricidal activity.

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Effectiveness of education for control of house dust mites and cockroaches in Seoul, Korea
Kyoung Yong Jeong, In-Yong Lee, Jongweon Lee, Han-Il Ree, Chein-Soo Hong, Tai-Soon Yong
Korean J Parasitol 2006;44(1):73-79.
Published online March 20, 2006
DOI: https://doi.org/10.3347/kjp.2006.44.1.73

We evaluated the efficacy of health education in reducing indoor arthropod allergens in Seoul. The mite control measures comprised the use of mite-proof mattress and pillow coverings, regular washing of potentially infested materials, maintenance of a low humidity, removal of carpets, and frequent vacuum cleaning. Cockroach control measures included trapping, application of insecticides, and protecting food. Of 201 homes enrolled in October 1999, 63 volunteers were included in a 2-year follow-up survey between April 2000 and January 2002. Before intervention, the density of mites/g of dust varied greatly; 27.1/g in children's bedding, 20/g in adult bedding, 7.2/g on the floors of children's bedrooms, 6.8/g in sofas, 5.9/g on the floors of adult's bedrooms, 3.9/g on living room floors, 3.7/g in carpets, and 1.9 mites/g on kitchen floors. The predominant mite species and house percentages infested were; Dermatophagoides farinae 93%, D. pteronyssinus 9%, and Tyrophagus putrescentiae 8%. Comparing 1999 and 2001 infestations, before and after 25 mo of education, mite abundance was reduced by 98%, from 23.7 to 0.57 mites/g of dust. In 1999, cockroaches were detected in 62% homes: 36% Blattella germanica and 35% Periplaneta spp., including 9% double infestations of B. germanica and P. americana. Following intervention, cockroach infestation rates decreased to 22% of houses in 2000 and 23% in 2001. We conclude that continuous and repetitive health education resulted in the effective control of domestic arthropods.

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    Allergy, Asthma & Immunology Research.2013; 5(2): 102.     CrossRef
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    Suhaili Zainal Abidin, Ho Tze Ming
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    Myung Hyun Sohn, Kyu-Earn Kim
    Allergy, Asthma & Immunology Research.2012; 4(5): 264.     CrossRef
  • Group 10 Allergens (Tropomyosins) from House-Dust Mites May Cause Covariation of Sensitization to Allergens from Other Invertebrates
    Rubaba Hamid Shafique, Muhammad Inam, Muhammad Ismail, Farhana Riaz Chaudhary
    Allergy & Rhinology.2012;[Epub]     CrossRef
  • Review on Ecology of House Dust Mites in Korea and Suggestion of a Standard Survey Method
    Tai-Soon Yong, Kyoung-Yong Jeong
    Pediatric Allergy and Respiratory Disease.2011; 21(1): 4.     CrossRef
  • Household Arthropod Allergens in Korea
    Tai-Soon Yong, Kyoung Yong Jeong
    The Korean Journal of Parasitology.2009; 47(Suppl): S143.     CrossRef
  • 10,261 View
  • 96 Download
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Brief Communication

Prevalence of arthropod antibodies in Korean patients with allergic rhinitis
Kyu-Yoon Hwang, Joon-Soo Park, Hyun-Cheol Ahn, Hae-Seon Nam
Korean J Parasitol 2001;39(2):197-199.
Published online June 30, 2001
DOI: https://doi.org/10.3347/kjp.2001.39.2.197

Arthropod antigens are main causative agents which induce allergic reponses in humans. However, little information is known about the prevalence of specific arthropod allergens in Koreans with allergic diseases. The current study was designed to determine the positive rates of arthropod antibodies by the Korean inhalant panel of MAST-CLA. One hundred sixty patients, who were diagnosed with allergic rhinitis from an out-patient center at the Soonchunhyang University Chunan Hospital, were studied between August 1998 to July 2000. The overall positive rate, at least more than one specific antibody of arthropods such as Dermatophagoides farinae (Df), Dermatophagoides pteronyssinus (Dp), and cockroach mix (Cm), was 46.9%. Each positive rate of Df, Dp, and Cm was 45.0%, 43.1%, and 8.8%, respectively. A significant agreement among arthropod allergens was observed (Df and Dp: 95.6%, Kappa = 0.911, P < 0.001). Our data supported the fact that arthropods were the most common allergens in Korean patients with allergic rhinitis; however, the MAST-CLA should be modified to increase specificity of arthropod allergens.

Citations

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  • Validity of MAST-CLA for diagnosis of arthropod allergy using receiver operating characteristic (ROC) analysis
    Joon-Soo Park, Hae-Seon Nam, Yong-Bae Kim, Young-Jin Choi, Sang-Han Lee, Sung-Ho Kim
    The Korean Journal of Parasitology.2007; 45(3): 239.     CrossRef
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Original Article
Monoclonal antibodies to recombinant Der p 2, a major house dust mite allergen: specificity, epitope analysis and development of two-site capture ELISA
Tai-Soon Yong, Sang-Mi Lee, Gab-Man Park, In-Yong Lee, Han-Il Ree, Kyung-Sup Kim, Sang-Hwan Oh, Jung-Won Park, Chein-Soo Hong
Korean J Parasitol 1999;37(3):163-169.
Published online September 20, 1999
DOI: https://doi.org/10.3347/kjp.1999.37.3.163

House dust mite allergens have been well established as sensitizing agents that are important in the induction of allergic diseases. In order to analyze epitopes of the allergen and to develop a quantitative method of the allergen exposure, monoclonal antibodies against a recombinant Der p 2 (rDer p 2), one of the major allergens of Dermatophagoides pteronyssinus, were produced. Four monoclonal antibodies produced were species-specific and did not cross-react to the D. farinae crude extract. Two of the monoclonal antibodies were found to be IgG1 and the others were IgM. For the analysis of epitopes, a Der p 2 cDNA encoding 126 amino acids (aa) was dissected into three fragments with several overlapping peptides, A (aa residues 1-49), B (44-93), and C fragment (84-126). Three monoclonal antibodies showed reactivities to the recombinant B fragment and to the full-length rDer p 2, but one monoclonal antibody reacted only with the full-length rDer p 2. Two-site capture ELISA was developed using two different monoclonal antibodies for quantitating Der p 2 in house dust. The sensitivity limit was 4 ng/ml with rDer p 2 and 8 ?g/ml with the D. pteronyssinus crude extract. The result suggested that the assay using monoclonal antibodies against rDer p 2 could be useful for the environmental studies and for the standardization of mite allergen extracts.

Citations

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  • Determination of immunogenic epitopes in major house dust mite allergen, Der p 2, via nanoallergens
    Jenna Sjoerdsma, Franklin Mejia, Basar Bilgicer
    Annals of Allergy, Asthma & Immunology.2022; 129(2): 231.     CrossRef
  • Mapping Mimotopes for House Dust Mite Allergen Der f 7 Using a Specific Monoclonal Antibody
    Jianglong Peng, Hao Yin, Ying Zhou, Haoyuan Jia, Yubao Cui
    Protein & Peptide Letters.2019; 26(3): 184.     CrossRef
  • Expression and purification of a major allergen, Pla a 1, from Platanus acerifolia pollen and the preparation of its monoclonal antibody
    Wei-Wei Ni, Wen Huang, De-Qin Wu, Yan-Jun Zhou, Chun-Mei Ji, Meng-Da Cao, Miao Guo, Jin-Lu Sun, Ji-Fu Wei
    Molecular Medicine Reports.2017; 16(3): 2887.     CrossRef
  • Review on Ecology of House Dust Mites in Korea and Suggestion of a Standard Survey Method
    Tai-Soon Yong, Kyoung-Yong Jeong
    Pediatric Allergy and Respiratory Disease.2011; 21(1): 4.     CrossRef
  • Monoclonal antibodies against recombinant Der f 3 reveal localization of Der f 3 in the gut and faecal pellets of Dermatophagoides farinae
    Zheng-ke Zhan, Kun-mei Ji, Xiao-yu Liu, Zhi-gang Liu, Meng Li, Jia-jie Chen, Jia-na Li, Shi Qiu
    Experimental and Applied Acarology.2010; 52(1): 63.     CrossRef
  • Household Arthropod Allergens in Korea
    Tai-Soon Yong, Kyoung Yong Jeong
    The Korean Journal of Parasitology.2009; 47(Suppl): S143.     CrossRef
  • Mimotopes identify conformational B-cell epitopes on the two major house dust mite allergens Der p 1 and Der p 2
    Krisztina Szalai, Jan Fuhrmann, Tea Pavkov, Michael Scheidl, Julia Wallmann, Kira H. Brämswig, Susanne Vrtala, Otto Scheiner, Walter Keller, Jean-Marie Saint-Remy, Dirk Neumann, Isabella Pali-Schöll, Erika Jensen-Jarolim
    Molecular Immunology.2008; 45(5): 1308.     CrossRef
  • Monoclonal antibodies to recombinant Der f 2 and development of a two‐site ELISA sensitive to major Der f 2 isoallergen in Korea
    K.Y. Jeong, H.S. Jin, S.H. Oh, C.‐S. Hong, I.‐Y. Lee, H.‐I. Ree, T.‐S. Yong
    Allergy.2002; 57(1): 29.     CrossRef
  • Monoclonal antibodies to recombinant Der f 2 and development of a two-site ELISA sensitive to major Der f 2 isoallergen in Korea
    K.Y. Jeong, H.S. Jin, S.H. Oh, C.-S. Hong, I.-Y. Lee, H.-I. Ree, T.-S. Yong
    Allergy.2002; 57(1): 29.     CrossRef
  • Localization of Der f 2 in the gut and fecal pellets of Dermatophagoides farinae
    K. Y. Jeong, I.‐Y. Lee, H.‐I. Ree, C.‐S. Hong, T.‐S. Yong
    Allergy.2002; 57(8): 729.     CrossRef
  • Localization of a major allergen, Der p 2, in the gut and faecal pellets of Dermatophagoides pteronyssinus
    Park, Lee, Lee, Ree, Kim, Hong, Yong
    Clinical & Experimental Allergy.2000; 30(9): 1293.     CrossRef
  • 9,138 View
  • 77 Download
  • Crossref