Giardia lamblia is a common enteric pathogen associated with diarrheal diseases. There are some reports of G. lamblia infection among different breeds of cattle in recent years worldwide. However, it is yet to know whether cattle in Jiangxi province, southeastern China is infected with G. lamblia. The objective s of the present study were to investigate the prevalence and examine the multilocus genotypes of G. lamblia in cattle in Jiangxi province. A total of 556 fecal samples were collected from 3 cattle breeds (dairy cattle, beef cattle, and buffalo) in Jiangxi province, and the prevalence and genotypes of G. lamblia were determined by the nested PCR amplification of the beta-giardin (bg) gene. A total of 52 samples (9.2%) were positive for G. lamblia. The highest prevalence of G. lamblia was detected in dairy cattle (20.0%), followed by that in beef cattle (6.4%), and meat buffalo (0.9%). Multilocus sequence typing of G. lamblia was performed based on sequences of the bg, triose phosphate isomerase and glutamate dehydrogenase loci, and 22, 42, and 52 samples were amplifiable, respectively, forming 15 MLGs. Moreover, one mixed G. lamblia infection (assemblages A and E) was found in the present study. Altogether, 6 novel assemblage E subtypes (E41*-E46*) were identified for the first time. These results not only provided baseline data for the control of G. lamblia infection in cattle in this southeastern province of China, but also enriched the molecular epidemiological data and genetic diversity of G. lamblia in cattle.
Citations
Citations to this article as recorded by
Global prevalence of Giardia infection in nonhuman mammalian hosts: A systematic review and meta-analysis of five million animals Kareem Hatam-Nahavandi, Ehsan Ahmadpour, Milad Badri, Aida Vafae Eslahi, Davood Anvari, David Carmena, Lihua Xiao, Aysegul Taylan Ozkan PLOS Neglected Tropical Diseases.2025; 19(4): e0013021. CrossRef
Investigation of Infection of Enterocytozoon bieneusi and Giardia duodenalis in Beef Cattle in Yunnan, China Fan Yang, Wenjie Cheng, Jianfa Yang, Junjun He, Liujia Li, Fengcai Zou, Fanfan Shu Veterinary Sciences.2025; 12(6): 552. CrossRef
An Epidemiology Study on Distribution of Giardia lamblia in Incheon City Su Jin Park, Ju Hee Kim, Hyeon-Jeong Go, Nam Yee Kim, Sung Suck Oh, Young Woo Gong, MunJu Kwon, Jeong-An Gim, Soo-Son Lim Biomedical Science Letters.2025; 31(2): 218. CrossRef
Genetic Characterization and Zoonotic Potential of Cryptosporidium spp. and Giardia duodenalis in Cattle From Northeast China Qile Yu, Sining Chen, Xichen Zhang, Qi Zhao, Mengfei Xu, Jianhua Li, Pengtao Gong, Xiaocen Wang, Xin Li, Xu Zhang, Nan Zhang, Xiangrui Li Transboundary and Emerging Diseases.2025;[Epub] CrossRef
Molecular Epidemiology of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Guizhou Angus Calves: Dominance of Angus Cattle-Adapted Genotypes and Zoonotic Potential of E. bieneusi Peixi Qin, Zhuolin Tao, Kaizhi Shi, Jiaxian Zhao, Bingyan Huang, Hui Liu, Chunqun Wang, Jigang Yin, Guan Zhu, Simone M. Cacciò, Min Hu Microorganisms.2025; 13(8): 1735. CrossRef
First report of Giardia duodenalis in dairy cattle and beef cattle in Shanxi, China Li Zhao, Yan Wang, Mingyuan Wang, Shan Zhang, Lifeng Wang, Zhansheng Zhang, Hailiang Chai, Chao Yi, Wenjun Fan, Yonghong Liu Molecular Biology Reports.2024;[Epub] CrossRef
Prevalence and molecular characterization of Giardia duodenalis in dairy cattle in Central Inner Mongolia, Northern China Li Zhao, Zhan-Sheng Zhang, Wen-Xiong Han, Bo Yang, Hai-Liang Chai, Ming-Yuan Wang, Yan Wang, Shan Zhang, Wei-Hong Zhao, Yi-Min Ma, Yong-Jie Zhan, Li-Feng Wang, Yu-Lin Ding, Jin-Ling Wang, Yong-Hong Liu Scientific Reports.2023;[Epub] CrossRef
Meta-Analysis of the Prevalence of Giardia duodenalis in Cattle in China Xiang-Zhu Meng, Chao Kang, Jiaqi Wei, He Ma, Gang Liu, Jin-Ping Zhao, Hong-Shun Zhang, Xin-Bo Yang, Xiang-Yu Wang, Li-Hua Yang, Hong-Li Geng, Hongwei Cao Foodborne Pathogens and Disease.2023; 20(1): 17. CrossRef
Giardia duodenalis in Hu sheep: occurrence and environmental contamination on large-scale housing farms Qianming Zhao, Chenyang Lu, Zhiyang Pei, Pihong Gong, Junqiang Li, Fuchun Jian, Bo Jing, Meng Qi, Changshen Ning Parasite.2023; 30: 2. CrossRef
Prevalence and Molecular Characterization of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in Diarrheic and Non-Diarrheic Calves from Ningxia, Northwestern China Haihui Gao, Gaoxing Liang, Na Su, Qirui Li, Dong Wang, Jiandong Wang, Long Zhao, Xiaodong Kang, Kangkang Guo Animals.2023; 13(12): 1983. CrossRef
Microbial Risks Caused by Livestock Excrement: Current Research Status and Prospects Rashidin Abdugheni, Li Li, Zhen-Ni Yang, Yin Huang, Bao-Zhu Fang, Vyacheslav Shurigin, Osama Abdalla Abdelshafy Mohamad, Yong-Hong Liu, Wen-Jun Li Microorganisms.2023; 11(8): 1897. CrossRef
Multilocus Genotyping of Giardia duodenalis Occurring in Korean Native Calves Sang-Ik Oh, Suk-Han Jung, Han-Kyoung Lee, Changyong Choe, Tai-Young Hur, Kyoung-Min So Veterinary Sciences.2021; 8(7): 118. CrossRef
The objective of this study was to genetically characterize isolates of Giardia duodenalis and to determine if zoonotic potential of G. duodenalis could be found in stray cats from urban and suburban environments in Guangzhou, China. Among 102 fresh fecal samples of stray cats, 30 samples were collected in Baiyun district (urban) and 72 in Conghua district (suburban). G. duodenalis specimens were examined using light microscopy, then the positive specimens were subjected to PCR amplification and subsequent sequencing at 4 loci such as glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), β-giardin (bg), and small subunit ribosomal RNA (18S rRNA) genes. The phylogenetic trees were constructed using obtained sequences by MEGA5.2 software. Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district. Among the 10 positive samples, 9 were single infection (8 isolates, assemblage A; 1 isolate, assemblage F) and 1 sample was mixed infection with assemblages A and C. Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI except for 1 isolate (CHC83). These findings not only confirmed the occurrence of G. duodenalis in stray cats, but also showed that zoonotic assemblage A was found for the first time in stray cats living in urban and suburban environments in China.
Citations
Citations to this article as recorded by
Global prevalence of Giardia infection in nonhuman mammalian hosts: A systematic review and meta-analysis of five million animals Kareem Hatam-Nahavandi, Ehsan Ahmadpour, Milad Badri, Aida Vafae Eslahi, Davood Anvari, David Carmena, Lihua Xiao, Aysegul Taylan Ozkan PLOS Neglected Tropical Diseases.2025; 19(4): e0013021. CrossRef
Molecular characterization of Cryptosporidium spp. and Giardia duodenalis in pet cats in Henan Province, central China Liangliang Li, Yuzhen Sui, Xinmiao Li, Pengtao Song, Guizhen Chen, Hu Liu, Shoujun Zuo, Jinjie Guo, Qiong Wang, Qiyuan Sun, Hongyu Dai, Junqiang Li, Tao Zhang, Fang Liu, Longxian Zhang, Haiju Dong Acta Tropica.2024; 254: 107188. CrossRef
Cryptosporidium and Giardia in cats and dogs: What is the real zoonotic risk? Amanda D. Barbosa, Siobhon Egan, Yaoyu Feng, Lihua Xiao, Una Ryan Current Research in Parasitology & Vector-Borne Diseases.2023; 4: 100158. CrossRef
Genetic characterization of Cryptosporidium spp. and Giardia duodenalis in dogs and cats in Guangdong, China Jiayu Li, Xiaoyu Dan, Kexin Zhu, Na Li, Yaqiong Guo, Zezhong Zheng, Yaoyu Feng, Lihua Xiao Parasites & Vectors.2019;[Epub] CrossRef
Giardia in a selected population of dogs and cats in Germany – diagnostics, coinfections and assemblages M.F. Sommer, P. Rupp, M. Pietsch, A. Kaspar, P. Beelitz Veterinary Parasitology.2018; 249: 49. CrossRef
Prevalence and risk factors of Giardia duodenalis in domestic rabbbits (Oryctolagus cuniculus) in Jilin and Liaoning province, northeastern China Jing Jiang, Jian-Gang Ma, Nian-Zhang Zhang, Peng Xu, Guangyu Hou, Quan Zhao, Xiao-Xuan Zhang Journal of Infection and Public Health.2018; 11(5): 723. CrossRef
Development of T m -shift genotyping method for detection of cat-derived Giardia lamblia Weida Pan, Yeqi Fu, Auwalu Yusuf Abdullahi, Mingwei Wang, Xianli Shi, Fang Yang, Xingang Yu, Xinxin Yan, Pan Zhang, Jianxiong Hang, Guoqing Li Parasitology Research.2017; 116(4): 1151. CrossRef
Giardia duodenalis Infections in Humans and Other Animals in China Junqiang Li, Haiyan Wang, Rongjun Wang, Longxian Zhang Frontiers in Microbiology.2017;[Epub] CrossRef
Genotypes of Cryptosporidium spp., Enterocytozoon bieneusi and Giardia duodenalis in dogs and cats in Shanghai, China Hailing Xu, Yue Jin, Wenxian Wu, Pei Li, Lin Wang, Na Li, Yaoyu Feng, Lihua Xiao Parasites & Vectors.2016;[Epub] CrossRef
A Multiplex PCR for Simultaneous Detection of Three Zoonotic ParasitesAncylostoma ceylanicum,A. caninum,andGiardia lambliaAssemblage A Wei Hu, Sheng Wu, Xingang Yu, Auwalu Yusuf Abullahi, Meiran Song, Liping Tan, Zhen Wang, Biao Jiang, Guoqing Li BioMed Research International.2015; 2015: 1. CrossRef
First
Prev
