Cryptosporidium and Cyclospora are well-known coccidian protozoa that can cause waterborne and foodborne diarrheal illnesses. There have been a few reports regarding contamination in different vegetables with Cryptosporidium, but no data are available regarding the sources of Cyclospora infections in Korea. In the present study, we collected 6 kinds of vegetables (perilla leaves, winter-grown cabbages, chives, sprouts, blueberries, and cherry tomatoes) from July 2014 to June 2015, and investigated contamination by these 2 protozoa using multiplex quantitative real-time PCR. Among 404 vegetables, Cryptosporidium and Cyclospora were detected in 31 (7.7%) and 5 (1.2%) samples, respectively. In addition, Cryptosporidium was isolated from all 6 kinds of vegetables, whereas Cyclospora was detected in 4 kinds of vegetables (except perilla leaves and chives). Cryptosporidium (17.8%) and Cyclospora (2.9%) had the highest detection rates in chives and winter-grown cabbages, respectively. Cryptosporidium was detected all year long; however, Cyclospora was detected only from October to January. In 2 samples (sprout and blueberry), both Cryptosporidium and Cyclospora were detected. Further investigations using TaqI restriction enzyme fragmentation and nested PCR confirmed Cryptosporidium parvum and Cyclospora cayetanensis, respectively. In conclusion, we detected C. cayetanensis in vegetables for the first time in Korea. This suggests that screening should be employed to prevent these protozoal infections in Korea.
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Cryptosporidium and cryptosporidiosis: An update of Asian perspectives in humans, water and food, 2015–2025 Shahira Abdelaziz Ali Ahmed, Sonia Boughattas, Mohammad Reza Mahmoudi, Huma Khan, Simuzar Mamedova, Ardra Namboodiri, Frederick R. Masangkay, Panagiotis Karanis Current Research in Parasitology & Vector-Borne Diseases.2025; 8: 100311. CrossRef
Cyclospora in humans, animals, fresh produce and water in China: implications for host specificity of Cyclospora species and zoonotic transmission of C. cayetanensis Kangli Feng, Yaqiong Guo, Na Li, Lihua Xiao, Yaoyu Feng One Health Advances.2025;[Epub] CrossRef
Cryptosporidium and agriculture: A review Eleni Golomazou, Simuzer Mamedova, Aida Vafae Eslahi, Panagiotis Karanis Science of The Total Environment.2024; 916: 170057. CrossRef
Unveiling risks in healthy food: Vegetables and fruits are linked to the distribution chain of protozoan parasites Aida Vafae Eslahi, Simuzer Mamedova, Reghaissia Nassiba, Panagiotis Karanis Food Microbiology.2024; 123: 104592. CrossRef
Food and Waterborne Cryptosporidiosis from a One Health Perspective: A Comprehensive Review Munwar Ali, Yaru Ji, Chang Xu, Qazal Hina, Usama Javed, Kun Li Animals.2024; 14(22): 3287. CrossRef
Loop mediated isothermal amplification for detection of foodborne parasites: A journey from lab to lab-on-a-chip Fatemeh Mahdavi Abhari, Maryam Niyyati, Hamid Assadzadeh Aghdaei, Hamed Mirjalali Food Control.2023; 143: 109251. CrossRef
Health risks of Cryptosporidium and Giardia in the application of surface water and septic tank effluent in Chinese agriculture: Impact on cancer patients identified by quantitative microbial risk assessment Qian Huang, Shan Huang, Weijie Kuang, Jianghui Yi, Shunxin Xiao, Feng Zhao, Guosheng Xiao Food Microbiology.2023; 111: 104213. CrossRef
Development of a new multiplex PCR to detect fecal coccidian parasite Manish Katiyar, Reena Gulati, Nonika Rajkumari, Rakesh Singh Indian Journal of Gastroenterology.2023; 42(2): 241. CrossRef
Contamination of fresh produce sold on the Italian market with Cyclospora cayetanensis and Echinococcus multilocularis Alessandra Barlaam, Tamirat T. Temesgen, Kristoffer R. Tysnes, Laura Rinaldi, Nicola Ferrari, Anna R. Sannella, Giovanni Normanno, Simone M. Cacciò, Lucy J. Robertson, Annunziata Giangaspero Food Microbiology.2021; 98: 103792. CrossRef
Detection of Cyclospora cayetanensis on bagged pre-cut salad mixes within their shelf-life and after sell by date by the U.S. food and drug administration validated method Sonia Almeria, Alicia Shipley Food Microbiology.2021; 98: 103802. CrossRef
Diverse Genotypes and Species of Cryptosporidium in Wild Rodent Species from the West Coast of the USA and Implications for Raw Produce Safety and Microbial Water Quality Xunde Li, Edward Robert Atwill Microorganisms.2021; 9(4): 867. CrossRef
Detection of Cyclospora cayetanensis, Echinococcus multilocularis, Toxocara spp. and microsporidia in fresh produce using molecular methods: – A review B. Bartosova, B. Koudela, I. Slana Food and Waterborne Parasitology.2021; 23: e00124. CrossRef
Causes of acute gastroenteritis in Korean children between 2004 and 2019 Eell Ryoo Clinical and Experimental Pediatrics.2021; 64(6): 260. CrossRef
An Epidemiological and Diagnostic Study of Cyclospora Cayetanensis Parasite in Anbar Province - Iraq S S Shahatha, S A Alkubaisy, M O Mousa IOP Conference Series: Earth and Environmental Science.2021; 904(1): 012026. CrossRef
Evaluation of the U.S. Food and Drug Administration validated molecular method for detection of Cyclospora cayetanensis oocysts on fresh and frozen berries Angela Assurian, Helen Murphy, Laura Ewing, Hediye Nese Cinar, Alexandre da Silva, Sonia Almeria Food Microbiology.2020; 87: 103397. CrossRef
A Molecular Tool for Rapid Detection and Traceability of Cyclospora cayetanensis in Fresh Berries and Berry Farm Soils Carolina N. Resendiz-Nava, Guadalupe E. Orozco-Mosqueda, Edmundo M. Mercado-Silva, Susana Flores-Robles, Hilda V. Silva-Rojas, Gerardo M. Nava Foods.2020; 9(3): 261. CrossRef
Parasite detection in food: Current status and future needs for validation Rachel M. Chalmers, Lucy J. Robertson, Pierre Dorny, Suzanne Jordan, Age Kärssin, Frank Katzer, Stéphanie La Carbona, Marco Lalle, Brian Lassen, Ivona Mladineo, Miroslaw Rozycki, Ewa Bilska-Zajac, Gereon Schares, Anne Mayer-Scholl, Chiara Trevisan, Kristo Trends in Food Science & Technology.2020; 99: 337. CrossRef
Detection of Cryptosporidium oocysts and Giardia cysts in vegetables from street markets from the Qinghai Tibetan Plateau Area in China Xiuping Li, Xueyong Zhang, Yingna Jian, Geping Wang, Liqing Ma, Chad Schou, Panagiotis Karanis Parasitology Research.2020; 119(6): 1847. CrossRef
Comparison of commercial and in-house real-time PCR platforms for 15 parasites and microsporidia in human stool samples without a gold standard Thomas Köller, Andreas Hahn, Enkhtsetseg Altangerel, Jaco J. Verweij, Olfert Landt, Simone Kann, Denise Dekker, Jürgen May, Ulrike Loderstädt, Andreas Podbielski, Hagen Frickmann Acta Tropica.2020; 207: 105516. CrossRef
Prevalence of Cryptosporidium and Giardia in vegetables in Iran: a nineteen-years meta-analysis review Ehsan Javanmard, Elnaz Sadat Mirsamadi, Meysam Olfatifar, Erfan Ghasemi, Fatemeh Saki, Hamed Mirjalali, Mohammad Reza Zali, Panagiotis Karanis Journal of Environmental Health Science and Engineering.2020; 18(2): 1629. CrossRef
Detection of human intestinal protozoan parasites in vegetables and fruits: a review Junqiang Li, Zhenzhen Wang, Md Robiul Karim, Longxian Zhang Parasites & Vectors.2020;[Epub] CrossRef
Human cyclosporiasis Annunziata Giangaspero, Robin B Gasser The Lancet Infectious Diseases.2019; 19(7): e226. CrossRef
Simultaneous detection of four protozoan parasites on leafy greens using a novel multiplex PCR assay Karen Shapiro, Minji Kim, Veronica B. Rajal, Michael J. Arrowood, Andrea Packham, Beatriz Aguilar, Stefan Wuertz Food Microbiology.2019; 84: 103252. CrossRef
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Cyclospora cayetanensis and Cyclosporiasis: An Update Sonia Almeria, Hediye N. Cinar, Jitender P. Dubey Microorganisms.2019; 7(9): 317. CrossRef
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Parasite contamination of berries: Risk, occurrence, and approaches for mitigation Tamirat Tefera, Kristoffer R. Tysnes, Kjersti Selstad Utaaker, Lucy J. Robertson Food and Waterborne Parasitology.2018; 10: 23. CrossRef
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