The objective of this study was to genetically characterize isolates of Giardia duodenalis and to determine if zoonotic potential of G. duodenalis could be found in stray cats from urban and suburban environments in Guangzhou, China. Among 102 fresh fecal samples of stray cats, 30 samples were collected in Baiyun district (urban) and 72 in Conghua district (suburban). G. duodenalis specimens were examined using light microscopy, then the positive specimens were subjected to PCR amplification and subsequent sequencing at 4 loci such as glutamate dehydrogenase (gdh), triose phosphate isomerase (tpi), β-giardin (bg), and small subunit ribosomal RNA (18S rRNA) genes. The phylogenetic trees were constructed using obtained sequences by MEGA5.2 software. Results show that 9.8% (10/102) feline fecal samples were found to be positive by microscopy, 10% (3/30) in Baiyun district and 9.7% (7/72) in Conghua district. Among the 10 positive samples, 9 were single infection (8 isolates, assemblage A; 1 isolate, assemblage F) and 1 sample was mixed infection with assemblages A and C. Based on tpi, gdh, and bg genes, all sequences of assemblage A showed complete homology with AI except for 1 isolate (CHC83). These findings not only confirmed the occurrence of G. duodenalis in stray cats, but also showed that zoonotic assemblage A was found for the first time in stray cats living in urban and suburban environments in China.
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Global prevalence of Giardia infection in nonhuman mammalian hosts: A systematic review and meta-analysis of five million animals Kareem Hatam-Nahavandi, Ehsan Ahmadpour, Milad Badri, Aida Vafae Eslahi, Davood Anvari, David Carmena, Lihua Xiao, Aysegul Taylan Ozkan PLOS Neglected Tropical Diseases.2025; 19(4): e0013021. CrossRef
Molecular characterization of Cryptosporidium spp. and Giardia duodenalis in pet cats in Henan Province, central China Liangliang Li, Yuzhen Sui, Xinmiao Li, Pengtao Song, Guizhen Chen, Hu Liu, Shoujun Zuo, Jinjie Guo, Qiong Wang, Qiyuan Sun, Hongyu Dai, Junqiang Li, Tao Zhang, Fang Liu, Longxian Zhang, Haiju Dong Acta Tropica.2024; 254: 107188. CrossRef
Cryptosporidium and Giardia in cats and dogs: What is the real zoonotic risk? Amanda D. Barbosa, Siobhon Egan, Yaoyu Feng, Lihua Xiao, Una Ryan Current Research in Parasitology & Vector-Borne Diseases.2023; 4: 100158. CrossRef
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Giardia duodenalis Infections in Humans and Other Animals in China Junqiang Li, Haiyan Wang, Rongjun Wang, Longxian Zhang Frontiers in Microbiology.2017;[Epub] CrossRef
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A Multiplex PCR for Simultaneous Detection of Three Zoonotic ParasitesAncylostoma ceylanicum,A. caninum,andGiardia lambliaAssemblage A Wei Hu, Sheng Wu, Xingang Yu, Auwalu Yusuf Abullahi, Meiran Song, Liping Tan, Zhen Wang, Biao Jiang, Guoqing Li BioMed Research International.2015; 2015: 1. CrossRef
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