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Trypanosome Glycosylphosphatidylinositol Biosynthesis

Yeonchul Hong1, Taroh Kinoshita2
The Korean Journal of Parasitology 2009;47(3):197-204.
Published online: August 28, 2009

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Corresponding author (ychong@knu.ac.kr)
• Received: May 9, 2009   • Revised: July 30, 2009   • Accepted: July 30, 2009

Copyright © 2009 by The Korean Society for Parasitology

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Trypanosome Glycosylphosphatidylinositol Biosynthesis
Korean J Parasitol. 2009;47(3):197-204.   Published online August 28, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.3.197
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Trypanosome Glycosylphosphatidylinositol Biosynthesis
Korean J Parasitol. 2009;47(3):197-204.   Published online August 28, 2009
DOI: https://doi.org/10.3347/kjp.2009.47.3.197
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Trypanosome Glycosylphosphatidylinositol Biosynthesis
Image Image
Fig. 1 The structure of GPI anchor of VSG from bloodstream form of trypanosome.
Fig. 2 The GPI biosynthesis pathways of T. brucei and mammalian cells. The uppermost row indicates the pathway of the procyclic form of T. brucei. The middle row represents the pathway of the bloodstream form and the lowermost row depicts the pathway of mammalian cells. The reactions indicated by the circled numbers (①-⑧) correspond to those described in the text. The involved protein(s) of each step are summarized in Table 1.

Fig. 1

Fig. 2

Trypanosome Glycosylphosphatidylinositol Biosynthesis
Step† Enzyme Gene
Mammals T. brucei ① GPI-GlcNAc transferase PIG-A PIG-H PIG-C GPI1(PIG-Q) PIG-P DPM2 PIG-Y ② de-N-acetylase PIG-L TbGPI12 PIG-L TbGPI12 ③ Acyltransferase PIG-W ④ GPI-α1-4mannosyltransferase I (MT-I) PIG-M PIG-X GPI-α1-6mannosyltransferase I (MT-II) PIG-V GPI-α1-2mannosyltransferase I (MT-III) PIG-B TbGPI10 ⑤ Ethanolamine-P-transferase (to the 1st mannose) PIG-N Ethanolamine-P-transferase (to the 3rd mannose) PIG-O Ethanolamine-P-transferase (to the 2nd mannose) PIG-F GPI7(GPI-G) ⑥ GPI inositol deacylase PGAP1 TbGPIdeAc TbGPIdeAc2
⑦ Fatty acid transferase (lipid remodeling) PGAP2 TbGup1
⑧ GPI transamidase GPI8 TbGPI8 GAA1 TbGaa1 PIG-S (TAA1)* PIG-T TbGPI16 PIG-U (TAA2)*
Table 1. Comparison of trypanosome and mammalian genes involved in GPI biosynthesis

Reaction steps correspond to those indicated in Fig. 2.

Table 1.

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