A characteristic feature of the tegument of Class Cestoda tapeworms is that it participates in the absorption of nutrients, excretion of waste metabolites, and protection from host digestive enzymes [2-4]. Ultrastructurally, the tegument of spargana is covered with numerous microtriches and glandular cells used for migration of the worm in the host [5,6]. This tegument is involved in initial antigenic contact with the host. Appropriately, potent antigenic molecules for host-generated antibodies are located in the outer layer of spargana [7]. In seeking an antibody-mediated preventive strategy for sparganosis, acquisition of the pure tegument or a part of the tegument, for example, the syncytial layer would be advantageous. The present study was performed to selectively separate the syncytial layer of the tegument using a high concentration of urea.

Spargana were collected from subcutaneous tissues of naturally infected snakes in Korea. After washing with sterile physiologic saline, the worms were stored at 4℃ until used to infection. Spargana from snakes were infected orally to BALB/c mice. After 1 month, infected mice were killed and spargana were collected from the subcutaneous tissues. Worms were individually added to a culture dish containing RPMI medium supplemented with 3 M urea, 1 mM EDTA, and 30% (v/v) fetal bovine serum. After incubation at 37℃ for 3 hr, spargana was fixed in 10% neutral buffered formalin and the fixed worms were processed by routine histological procedures followed by staining with hematoxylin and eosin. All reagents used in this study were purchased from Sigma-Aldrich (St. Louis, Missouri, USA).

Initially an effective urea concentration was identified. At 1 M urea, worms were still active and separation of the syncytial layer did not occur. At least 2 M urea was needed to achieve a successful separation (data not shown). Separation of the syncytial layer from the parenchyma of spargana was evident beginning from 1 hr after incubation in 3 M urea and the separation continued until 3 hr of observation (Fig. 1A). At 3 hr after incubation, the syncytial layer was easily collected by rolling or scratching the sparganum using a syringe needle or a micro-forcep (Fig. 1B). Hematoxylin and eosin staining demonstrated a clear separation of the syncytial layer of the tegument from the parenchyma (Fig. 1D).

Urea is a commonly used chemical denaturant of proteins, and is well established to be a tool for the study of biological membranes. Generally, protein solubility increases with increasing urea concentrations [8]. Although precise details of ureamediated separation of the syncytial layer remain unknown, it is conceivable that it may involve the ready permeation of the uncharged, low molecular weight urea through the syncytial layer and solubilization of proteins anchoring the syncytial layer on the parenchymal layer of the sparganum.

Serologic diagnosis of human sparganosis relies on ELISA [9] or immunoblot [10] detection of host serum antibodies. Various kinds of antigenic proteases are known to be present in the sparganum. They include excretory-secretory proteins with a molecular weight of 31/36 kDa [11], 27 kDa cathepsin S-like protease [12], and 53 kDa neutral protease [13]. These proteases, which are secreted from the tegument of spargana, potently influence host immune processes that include IgG degradation and specific IgE responses [12,13]. In this respect, studies of the tegumental layer of Class Cestoda tapeworms have focused on the ultrastructure of specific structures like microtriches [6]. The present study offers the means of separating the syncytial layer of the tegument, which opens a door for specific studies of the tegument physiology and antigenicity.