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Incorporation of exogenous D-glucose-1-14C and D-manose-1-14C into glycogen and lactate excreted by Angiostrongylus cantonensisin vitro
Dong Wik Choi
Department of Parasitology, Kyungpook National University, School of Medicine, Taegu, Korea.
Abstract
The amount of labelled glucose incorporated into glycogen is 6 times as much as that of labelled mannose by the female worms. They excreted lactate of labelled glucose-origin 3-4 times as much as that of labelled mannose-origin. The amount of labelled glucose incorprated in worm bodies is about 3 times as much as that of labelled mannose. Male worms absorbed a half amount of glucose by female worms.
Paralelled with radioactivity studies mentioned above, chemical determinations of sugars, lactate, and glycogen were performed on the media prior and posterior to incubation and the worms. The results obtained from chemical determinations fairly agreed with those on lactate production and sugar consumption from radioactivity studies, but not on glycogen content of worms after incubation. Chemical determination shows no glycogen synthesis in worms incubated in mannose media while the radioactivity of labelled mannose was detected, though it is very low in activity, from glycogen of worms in the media.
Coupled with previously reported findings, results from the present study show mannose to be far less significant in the carbohydrate metabolism of Angiostrongylus cantonensis as compared with glucose.
Tables
Table 1 Fractionation procedures and fractions with abbreviations
Table 2 Radioactivity of fractions and media prior and posterior to incubation
Table 3 Incorporation of glucose and mannose into of A. cantonensis incubated in Tyrode's sol. with Iabelled sugars
Table 4 Incorporation of glucose and mannose into lactate excreted by of A. cantonensis incubated in Tyrode's sol. with Iabelled sugars
Table 5 Absorption of exogenous glucose and mannose by A. cantonensis incubated in Tyrode's sol. with Iabelled sugars
Table 6 Sugar consumption, lactate production, and glycogen content of A. cantonensis females by chemical determinations
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