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A comparison of the efficiency of the three egg count techniques
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Korean J Parasito > Volume 10(2):1972 > Article

Original Article
Korean J Parasitol. 1972 Aug;10(2):90-94. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1972.10.2.90
Copyright © 1972 by The Korean Society for Parasitology
A comparison of the efficiency of the three egg count techniques
O. R. Lee,W. K. Lee,B. H. Yun and K. M. Lee
Department of Parasitology, Catholic Medical College, Seoul, Korea.
Abstract

Usually egg count technique has been employed to know the intensity of infestation of human intestinal helminths and to elucidate the effects of anthelmintics. Comparing the three egg count methods - Stoll's egg dilution method, Beaver's direct smear method and FHK counting board method - the authors reached the following results:

1) As the result of application of the three methods to 75 Ascaris egg positives and 95 Trichocephalus egg positives, in the case of Ascaris eggs, the egg detection rate was 99% by Stoll's method, 97% by Beaver's method and was lowest by FHK method at 79%. Meanwhile, in Trichocephalus eggs, Stoll's method revealed 92%, Beaver's method 95%, and FHK method 95%, there being no noticeable differences.

2) With regard to EPG, in counting Ascaris eggs, Stoll's method showed a value closest to the actual number of eggs, and the value revealed by FHK method was smaller than the actual number.

3) As the result of comparing the coefficiencies of variation of the three egg counting methods, both in Ascaris eggs and Trichocephalus eggs, the FHK method showed the smallest, followed by Stoll's method and Beaver's method.

Tables


Table 1
Incidence of helminths and protozoa among the Catholic Medical Colleagues and his families


Table 2
Means of EPG and detecting ratio by three egg count methods on A. lumbricoides and T. trichiurus


Table 3
Mean of egg counts and coefficients of variation for three egg count methods of A. lumbricoides and T. trichiurus

References
1. Beaver PC. Quantitative hookworm diagnosis by direct smear. J Parasitol 1949;35(2):125–135.
  
2. Keller AE. Am J Hyg 1934;20(2):307–316.
3. Soh CT, et al. Yonsei Med J 1961;2:31–41.
4. Stoll RN, et al. Am J Hyg 1926;6:134–145.
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