Immobilization-agglomeration reaction for the diagnosis of <i>Trichomonas vaginalis</i> infection

, Chung, Pyung Rim

doi:1970.8.1.13

Korean J Parasito > Volume 8(1):1970 > Article

Original Article


Korean J Parasitol. 1970 Apr;8(1):13-21. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1970.8.1.13
Copyright © 1970 by The Korean Society for Parasitology


Immobilization-agglomeration reaction for the diagnosis of Trichomonas vaginalis infection
Pyung Rim Chung
Department of Parasitology, Yonsei University Medical College, Seoul, Korea.


Abstract
The Trichomonas immobilization-agglomeration reaction was studied using the sera from women with vaginal trichomoniasis and from rabbits inoculated with cultures of T. vaginalis. It was found that the greatest amount of immobilization occurred at 25 to 30 minutes, and that inactivation of the sera did not affect the results. An evidence of antigenic differences between T. vaginalis, T. hominis and C. albicans was confirmed. The immobilization reaction was positive in 80.3% of the 71 T. vaginalis positive cases, but in only 7.7% of the 77 persons of T. vaginalis negative cases. From the above results, it is suggested that immobilization reaction is a useful tool for the diagnosis of T. vaginalis infection.

Figures


	Fig. 1 Effect of T. vaginalis antiserum against T.vaginalis at its immobilizing and agglomerating titer of 1/16. ……… : Disintegration of cells. ↑ : Complete disintegration.


	Fig. 2 Immobility of T. vaginalis as a function of time and the concentration of human antiserum. — : antiserum, …… : control serum


	Fig. 3 Vaginal flagellates which move actively in normal human serum for control of the immobilization-agglomeration test (×400)


	Fig. 4 Vaginal flagellates which are immobilized and agglomerated in human antisera (×400)

Tables


	Table 1 Time and titer for 100% immobilization of T. vaginalis in rabbit antiserum


	Table 2 100% immobilization time according to the duration after T.vaginalis inoculation to the rabbit


	Table 3 Antigenic relationship between T. vaginalis and T. hominis after treatment with T. vaginalis antiserum for 30 minutes


	Table 4 Antigenic relationship between T. vaginalis and C. albicans after treatment with T. vaginalis antiserum


	Table 5 Immobility (%) of T. vaginalis as a function of time and concentration of human antiserum


	Table 6 Immobilization and agglomeration reaction (I.A.R.) of T. vaginalis in the inactivated or activated antisera


	Table 7 Result of I.A.R. for T. vaginalis with human sera


	Table 8 Sensitivity and specificity of I.A.R. for diagnosis of Trichomoniasis vaginalis

References


1.	Biagi F, Buentello L. Immobilization reaction for the diagnosis of amebiasis. Exp Parasitol 1961;11:188–190.

2.	Biagi F, Beltran F, Ortega PS. Remobilization of Entamoeba histolytica after exposure to immobilizing antibodies. Exp Parasitol 1966;18(1):87–91.

3.	Brown JA, Whitby JL. An immobilization test for amoebiasis. J Clin Pathol 1955;8(3):245–246.

4.	Cole BA, Kent JF. Immobilization of Endamoeba histolytica in vitro by antiserum produced in the rabbit. Proc Soc Exp Biol Med 1953;83(4):811–814.

5.	Craig CF. Amebiasis and the complement-fixation test. U S Armed Forces Med J 1950;1(11):1337–1342.

6.	Greif RL. Am J Trop Med 1947;27:131–134.

7.	Hong SO, et al. Yonsei Med J 1968;9:127–138.

8.	Karnaky KJ. Texas State J Med 1937;32:803–808.

9.	Kessel JF, et al. Am J Obst & Gynec 1940;39:1005–1014.

*10.*	Kessel JF, et al. Am J Trop Med Hyg 1965;14:540.

*11.*	McEntegart MG. J Path & Bact 1956;71:111.

*12.*	Nelson RA Jr, Mayer MM. Immobilization of Treponema pallidum in vitro by antibody produced in syphilitic infection. J Exp Med 1949;89(4):369–393.

*13.*	Prakash O, Tandon BN, Bhalla I, Ay AK, Vinayak VK. Indirect hemagglutination and ameba-immobilization tests and their evaluation in intestinal and extraintestinal amebiasis. Am J Trop Med Hyg 1969;18(5):670–675.

*14.*	Soh CT, et al. Yonsei Med J 1961;2:31–41.

*15.*	Stabler RM, et al. Am J Hyg 1941;34(c):114–118.

*16.*	Trussell RE, et al. Am J Obst Gynec 1942;44:292–295.

*17.*	Trussell RE. J Parasit Urbana 1946;32:563–569.

*18.*	Young RL. Trichomoniasis in the male. Rocky Mt Med J 1949;46(11):928–931.

*19.*	Zaman V. Studies on the Immobilization Reaction in the Genus Balantidium. Trans R Soc Trop Med Hyg 1964;58:255–259.