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An evaluation of cellophane thick smear technique for mass stool examination
Seung Yull Cho,Soon Hyung Lee,Han Jong Rim and Byong Seol Seo
Department of Parasitology and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.
Abstract
To evaluate the Kato's cellophane thick smear techcique in detection ability of various helminth ova, authors examined 1,843 nationwidely collected stool specimens by the two methods, formalin-ether technique and cellophane thick smear technique. And the results were analyzed.
1. The positive rates of following helminth ova were superior in cellophane thick smear technique to those of formalin-ether technique: Ascaris lumbricoides(62.3% : 51.1%), Taenia species (1.5% : 0.9%). The difference in A. lumbricoides ova detection is highly significant one. The overall positive rate for helminths ova was also higher in Kato's method (92.3% : 90.0%).
2. The positive fates of following helminth ova were infrior in cellophane thick smear technique to those of formalin-ether technique: Hookworm (5.8% : 14.6%), Trichostrongylus orientalis(3.2% : 17.8%), Clonorchis sinensis(8.3% : 11.0%), Metagonimus yokogawai (0.6% : 1.7%) and Hymenolepis nana(0.05% : 0.3%). The positive rate for the ova of Trichocephalus trichiurus by the two methods was incidentally same, 74.1%.
3. The causes of inferior positive rates in some helminth ova by cellophane thick smear technique were shortly discussed and suggests some opinions in improvement of detection ability.
Tables
Table 1 Result of stool examination by formalin-ether technique and cellophane thick smear technique
References
1.
Komiya Y, et al. Jpn J Parasit 1960;9(1):61–68.
2.
Lee KT, Kim CH, Park CT, Lee MY. [Cysticercosis and taeniasis in Chollapukdo Province]. Korean J Parasitol 1966;4(1):39–45.
3.
Martin LK, Beaver PC. Evaluation of Kato thick-smear technique for quantitative diagnosis of helminth infections. Am J Trop Med Hyg 1968;17(3):382–391.
4.
Ritchie LS. An ether sedimentation technique for routine stool examinations. Bull U S Army Med Dep 1948;8(4):326.
5.
Seo BS, Rim HJ, Loh IK, Lee SH, Cho SY, Park SC, Bae JW, Kim JH, Lee JS, Koo BY, Kim KS. [Study On The Status Of Helminthic Infections In Koreans]. Korean J Parasitol 1969;7(1):53–70.
6.
Saito M. Jpn J Parasit 1961;10(2):196–203.
7.
Uchida A, et al. Jpn J Parasit 1964;13(6):514–520.
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