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Parasit Host Dis > Volume 6(3):1968 > Article

Original Article
Korean J Parasitol. 1968 Dec;6(3):101-108. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1968.6.3.101
Copyright © 1968 by The Korean Society for Parasitology
The host-parasite relations of Clonorchis sinensis in the mouse - Studies on the development of C. sinensis in mouse -
Chung-Hwan Rhee and Byong-Seol Seo
Department of Parasitology and Institute of Endemic Diseases, College of Medcine, Seoul National University, Korea.

It is well established that guinea pig, rabbit and rat are equally susceptible to experimental infection with the Chinese liver fluke, Clonorchis sinensis. However, little work has been done on the studies of the host-parasite relationships between the liver fluke and mouse host. The experiments were undertaken to investigate the susceptibility, development and sexual maturity of Clonorchis sinensis in mouse host.

The metacercariae of C. sinensis used in these experiments were isolated from the fish, Pseudorasbora parva by digestion technic. And in all these studies the mice weighing around 20 g were commercially purchased and infected with the metacercariae under slightly narcotized condition with ether. The animals were starved overnight before infection. The mature metacercariae, e.g. 5, 10, 20, 30, 50, 200 were given orally into stomach by means of the polyethylene tubing (intramedic, Clay Adams, Inc.PE-90/S12) respectively to each mouse of groups ranging 4 to l5. After various period of infection, the mouse liver was first opened along the common bile duct and cut into small pieces for collecting and counting the worms. For the histopathological examination of the liver, the tissue specimens were fixed in formalin, sectioned and stained with haematoxylin-eosin. Another experiment was separately set up for the study of egg laying capacity of C. sinensis in mice. The following results were obtained:

1) Mice were successfully infected with metacercariae of C. sinensis in all cases of the groups except the group given 5 and 10 metacercariae, in which the infection was not established in one case of each group. Therefore it is assumed that mice are susceptible to infection with this worm. In an earlier period ot infection, the worms were found mostly in common bile duct and intrahepatic biliary passages with same rate, however in later period, they were seen rather in the intrahepatic biliary passages (73%) than in common bile duct and gall bladder. The recovery rate of fully matured adult worms (less than 10%) was lower than that of the average recovery rate of the worms in general (22.3%).

2) Under same age of infection or even in same host, the size of collected worms showed the great variations. Oral sucker was smaller in size than ventral sucker in the early stage of infection. After 11 days of infection it reversed. The posterior part of body length began to elongate since 5 days after infection, and therefore the ratio of antero-posterior part became l to 3.

Fully matured adult worms were only collected after 30 days of infection. The first positive appearance of eggs in feces was on the 17th and 18th day after infection. However, the egg-laying capacity in mouse host seemed to be stabilized since 30 days after infection.

3) Histopathologically, the cystic dilatation of medium to small biliary passages was noted and focal but diffusely scattered necrosis of the liver cells with scarce inflammatory cells was also observed as well as the hepatocellular degeneration, diffuse vascular congestions and adenomatous proliferationof bile duct.


Fig. 1
The number of worms found in each group mice experimentally infected with various number of metacercaria of C. sinensis

Fig. 2
Variations of the body length of the worms at various period of infections.

Fig. 3
Development of anterior and posterior body length at various periods of infections.

Plate: Fig. 1
Young worms in the lumen of bile duct.

Plate: Fig. 2
Marked cystic dilatation of the bile duct of mouse.

Plate: Fig. 3
11 day-old worm.

Plate: Fig. 4
Anterior and posterior parts of a 11 day old worm.

Plate: Fig. 5
Anterior and posterior parts of a 11 day old worm.

Plate: Fig. 6
Middle portion of a 29 day old worm, showing eggs in uterus, vitellaria with its duct, ovary with Mehlis' gland, spermatheca, anterior testis and intestinal ceca.

Plate: Fig. 7
Anterior and posterior parts of a 33 day old worm.

Plate: Fig. 8
Anterior and posterior parts of a 33 day old worm.


Table 1
Rates of infection and recovery and average worm burden in each group of mice, experimentally infected with the metacercariae of C. sinensis

Table 2
Number of worms found in various sites in the liver

Table 3
Measurements of the well developed worms of C. sinensis selected at various developmental stages (Unit: mm)

Table 4
Serial check of egg output of Clonorchis sinensis in mouse host infected with 20 metacercariae

1. Faust EC, et al. Am J Hyg Monographic Series 1927;8:283.
2. Komiya Y, Kawana-Tajimi T. The development of the excretory system of Clonorchis sinensis in its definitive host. Jpn J Med Sci Biol 1953;6(6):571–575.
3. Seo BS. Seoul Univ J Nat Sci 1958;7:1–15.
4. Wykoff DE. Studies on Clonorchis sinensis. III. The host-parasite relations in the rabbit and observations on the relative susceptibility of certain laboratory hosts. J Parasitol 1958;44(5):461–466.
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