| Home | E-Submission | Sitemap | Contact us |  
Korean J Parasito Search


Parasit Host Dis > Volume 3(3):1965 > Article

Original Article
Korean J Parasitol. 1965 Dec;3(3):127-131. English.
Published online Mar 20, 1994.  http://dx.doi.org/10.3347/kjp.1965.3.3.127
Copyright © 1965 by The Korean Society for Parasitology
An experimental study on uptake of Cr51 tagged red blood cells to Paragonimus westermani in definitive host
Suck Yong Kang, M.D.,Yong Soo Chun, M.D. and In Kyu Loh, M.D.
Department of Internal Medicine and Institute of Endemic Diseases, College of Medicine, Seoul National University, Korea.
School of Public Health, Seoul National University, Korea.

Authors carried out the experimental study to investigate whether the Paragonimus westermani would uptake the blood in the parasitic definitive host, and also tried for the amount and distribution of the blood which would be harbored in a worm body, and so on.

Experimental animals used in this study were dogs and cats, and they were infected with metacercariae of P. westermani. Several months later of the infection, Cr51 tagged red blood cells were administered to each animal, and the animals were sacrificed and autopsy was done after 24 or 48 hrs of the administration.

One part of the detected P. westermani adult worms of each animal were bred in Tyrode's solution and the others were killed by fixing in formalin solution.

By Well-type scintillation counter, the radioactivities were measured for the alive and killed worms and of the blood of each experimental animal which was collected at autopsy.

In conclusion, it was found that P. westermani worms uptake blood in parasitic definitive host, and amount of the blood harboring in a worm of 56 infection-days(size of worm; 4 × 3 × 1 mm) was 0.002 ml, 0.004 or 0.005 ml in 103 infection-days(size of worm: 8 × 5 × 3 mm) and 0.011 ml in 217 infection-days(size of worm: 9 × 7 × 4 mm). Almost of the whole blood uptaked in the worms was distributed in intestine. It was observed that the blood uptaked in the worm body was excreted continuously through the excretory pore, so that the radioactivity could not measured almost completely in cases of 24 hrs-breeding worms.


Table 1
Experimental materials and method of Cr51 tagged RBC infection

Table 2
Radioactivity in the P. westermani worms and blood

Table 3
Amount of blood contained in the P. westermani worm body(derived from Table 2)

Table 4
Respective radioactivities of intestine and other organs of the P. westermani worms

Table 5
Washing effect on the radioactivity of P. westermani worms of control dog steeped in radioactive blood

1. Pearson IG. Use of the chromium radioisotope 51Cr to estimate blood loss through ingestion by Fasciola hepatica. Exp Parasitol 1963;13:186–193.
2. Roche M, Perez-Gimenez ME, Layrisse, Di Prisco E. Gastrointestinal bleeding in hookworm infection; studies with radioactive chromium (Cr51); report of five cases. Am J Dig Dis 1957;2(5):265–277.
Editorial Office
Department of Molecular Parasitology, Samsung Medical Center, School of Medicine, Sungkyunkwan University,
2066 Seobu-ro, Jangan-gu, Suwon 16419, Gyeonggi-do, Korea.
Tel: +82-31-299-6251   FAX: +82-1-299-6269   E-mail: kjp.editor@gmail.com
About |  Browse Articles |  Current Issue |  For Authors and Reviewers
Copyright © 2023 by The Korean Society for Parasitology and Tropical Medicine.     Developed in M2PI