Studies on the intradermal test of Clonorchis peptide(CPT) Antigen 1. Preliminary report

Original Article


Korean J Parasitol. 1965 Jun;3(1):39-44. English. Published online Mar 20, 1994. http://dx.doi.org/10.3347/kjp.1965.3.1.39
Copyright © 1965 by The Korean Society for Parasitology


Studies on the intradermal test of Clonorchis peptide(CPT) Antigen 1. Preliminary report
Dong Wik Choi, M.D.,Sang Whon Lee, M.D.,Jung Man Kim, M.D. and Jong Taek Lee, M.D.
Department of Preventive Medicine, School of Medicine, Kyungpook National University, Korea.



Abstract
Polypeptide antigen as isolated from adult worms of Clonorchis sinensis by means of diluted HCl-extraction method. Preliminary intradermal examination with those polypeptide antigen(CPT) were performed on patients of clonorchiasis, paragonimiasis and metagonimiasis and the result of the intradermal test with CPT antigen were compared with those of Clonorchis protein fraction(CPF) and Veronal buffer saline extraction (VBS) antigen. The result of the examination were summerized as follows. 1. When 0.05 ml (1 microgram) of CPT was injected intradermally, the positive intradermal reaction appeared in 42.7 per cent (37 of 82) on the patients of clonorchiasis. 2. The sizes of the wheal and erythema with CPT antigen was smaller than that of CPF and VBS antigen. 3. Intradermal reaction with CPT antigen was carried out for other parasitic diseases, such as paragonimiasis and metagonimiasis. The result indicated that CPT antigen may be showed no cross reactions. 4. Clonorchiasis pateints who with negative reaction to CPT antigen, reacted negatively to CPF and VBS antigens.

Figures


	Fig. 1 Frequency Distribution of Enlarged wheal sizes in 82 Clonorchiasis patients.


	Fig. 2 Comparsion of the increased diameters of the wheals between CPE and CPT antigens.


	Fig. 3 Comparsion of the increased diameters of the wheals between VBS and CPT antigens.


	Fig. 4 Comparsion of the increased diameters of the wheals between VBS and CPF antigens.

Tables


	Table 1 Isolation of Peptide Antigen.


	Table 2 Results of qualitative analysis with CPT.


	Table 3 Cutaneous reaction to a C. sinensis Peptide Antigen (CPT) in person with clonorchiasis, paragonimiasis.Metagonimiasis and in non-infected controls.


	Table 4 Comparison of results of intradermal test with CPT, CPF and VBS(K) antigens in 82 Clonorchiasis patients.

References


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