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Original Article

Seroprevalence of Antibodies against Anisakis simplex Larvae among Health-Examined Residents in Three Hospitals of Southern Parts of Korea

Jung Kim1, Jin Ok Jo1, Seon Hee Choi2, Min Kyoung Cho2, Hak Sun Yu2, Hee Jae Cha1, Meesun Ock1
The Korean Journal of Parasitology 2011;49(2):139-144.
Published online: June 14, 2011

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Corresponding author (msock@ns.kosinmed.or.kr)
• Received: December 10, 2010   • Revised: April 3, 2011   • Accepted: April 11, 2011

© 2011, Korean Society for Parasitology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Seroprevalence of Antibodies against Anisakis simplex Larvae among Health-Examined Residents in Three Hospitals of Southern Parts of Korea
Korean J Parasitol. 2011;49(2):139-144.   Published online June 14, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.2.139
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Seroprevalence of Antibodies against Anisakis simplex Larvae among Health-Examined Residents in Three Hospitals of Southern Parts of Korea
Korean J Parasitol. 2011;49(2):139-144.   Published online June 14, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.2.139
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Seroprevalence of Antibodies against Anisakis simplex Larvae among Health-Examined Residents in Three Hospitals of Southern Parts of Korea
Image Image Image
Fig. 1 SDS-PAGE of excretory-secretory products (ES) from Anisakis simplex L3 larvae and somatic crude extract (CE). M: Molecular marker. Arrow: 130 kDa.
Fig. 2 Distribution of specific serum IgE against Anisakis simplex somatic crude extracts (CE) and excretory-secretory products (ES). It was expressed as the OD value of ELISA in residents of Geoje city, Masan city, and Busan Metropolitan city. Each point represents a single serum. Cut-off OD value is 0.10.
Fig. 3 Western blot analysis of Anisakis simplex L3 excretory-secretory products (A, C) and crude extracts (B) with sera of patients. A,B: sera from Geoje city, C: sera from Masan city. The 130 kDa protein (arrow) was detected in 10 sera among ELISA positive samples. M: Molecular marker.

Fig. 1

Fig. 2

Fig. 3

Seroprevalence of Antibodies against Anisakis simplex Larvae among Health-Examined Residents in Three Hospitals of Southern Parts of Korea
Age No. of subjects
ELISA positive
WBa positive
Total Female Male CEb ESc CE ES 10-19 34 23 11 4 3 0 0 20-29 107 67 40 6 5 0 1 30-39 81 54 27 5 6 0 4 40-49 71 26 45 3 4 0 1 50-59 55 17 38 2 1 1 1 60-69 55 15 40 1 3 0 1 70-79 45 29 16 4 7 1 0 80- 50 38 12 0 4 0 0 Total 498 269 229 25 33 (20d) 2e 8e
Table 1. Age group distribution of subjects and results of ELISA and western blot with Anisakis simplex L3 larva crude extract antigen and excretory-secretory antigen

Western blot,

crude extracts,

excretory-secretory product,

the number of positive females,

130 kDa band positive.

Table 1.

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