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Original Article

Development and Clinical Evaluation of a Rapid Serodiagnostic Test for Toxoplasmosis of Cats Using Recombinant SAG1 Antigen

Chom-Kyu Chong1,†, Wooseog Jeong2,†, Hak-Yong Kim1, Dong-Jun An2, Hye-Young Jeoung2, Jeong-Eun Ryu2, A-Ra Ko3, Yong-Joo Kim3, Sung-Jong Hong4, Zhaoshou Yang5, Ho-Woo Nam5
The Korean Journal of Parasitology 2011;49(3):207-212.
Published online: September 30, 2011

1Department of Biochemistry, College of Natural Science, Chungbuk National University, Cheongju, Chungbuk 361-763, Korea.

2Bacteriology and Parasitology Division, National Veterinary Research and Quarantine Service, Anyang 430-824, Korea.

3Institute of BioMedicals, Bioland Ltd., Ochang, Chungbuk 363-883, Korea.

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Corresponding author (howoo@catholic.ac.kr)

†Chom-Kyu Chong and Wooseog Jeong equally contributed as the first author.

• Received: April 25, 2010   • Revised: June 23, 2011   • Accepted: June 27, 2011

© 2011, Korean Society for Parasitology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Development and Clinical Evaluation of a Rapid Serodiagnostic Test for Toxoplasmosis of Cats Using Recombinant SAG1 Antigen
Korean J Parasitol. 2011;49(3):207-212.   Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.207
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Development and Clinical Evaluation of a Rapid Serodiagnostic Test for Toxoplasmosis of Cats Using Recombinant SAG1 Antigen
Korean J Parasitol. 2011;49(3):207-212.   Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.207
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Development and Clinical Evaluation of a Rapid Serodiagnostic Test for Toxoplasmosis of Cats Using Recombinant SAG1 Antigen
Image Image Image
Fig. 1 Diagram of TgRDT strip to detect antibodies against T. gondii. rTgAg was immobilized onto the T region and anti-mouse IgG was onto the C area of the strip. Conjugate pad contains rTgAg- and mouse IgG-conjugated gold colloid. ① sample pad; ② blood separation pad; ③ conjugate pad; ④ nitrocellulose membrane; ⑤ plastic backbone; ⑥ absorbance pad; ⑦ test line (T); and ⑧ control line (C).
Fig. 2 (A) Positive and negative results of the assembled TgRDT kit. (B) Immuno-reactivities of rSAG1, rSAG2, and rGRA6 to antibodies against T. gondii. ① rSAG1-applied; ② rSAG2-applied; and ③ rGRA6-applied.
Fig. 3 Western blot analysis of 5 specimens having different results between TgRDT and ELISA.

Fig. 1

Fig. 2

Fig. 3

Development and Clinical Evaluation of a Rapid Serodiagnostic Test for Toxoplasmosis of Cats Using Recombinant SAG1 Antigen
Positive sample (ID) Dilution fold S/P value Detection thresholds
TgRDT ELISA No. 1 (#33) 4 132 + + 16 125 + + 64 69 +w + 128 42 +w - 256 24 - - No. 2 (#141) 4 140 ++ + 16 98 + + 64 49 + - 128 25 +w - 256 16 - - No. 3 (#154) 4 134 + + 16 93 + + 64 38 +w - 128 23 - - Reference results TgRDT
ELISA
Positive Negative Total Positive Negative Total Positive 23 0 23 21 2 23 Negative 1 158 159 2 157 159 Total 24 158 182 23 159 182
Table 1. Analytical sensitivity of TgRDT and ELISA

There were no discrepancies between observations made by 2 technicians.

+, positive; +w, very weak positive; and -, negative.

Table 2. Clinical performance of TgRDT and ELISA

Table 1.

Table 2.

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