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Brief Communication

Lactobacillus acidophilus Contributes to a Healthy Environment for Vaginal Epithelial Cells

Woojin Pi1, Jae-Sook Ryu2, Jaesook Roh1
The Korean Journal of Parasitology 2011;49(3):295-298.
Published online: September 30, 2011

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Corresponding author (rohjaesook@hanyang.ac.kr)
• Received: June 7, 2011   • Revised: July 25, 2011   • Accepted: July 30, 2011

© 2011, Korean Society for Parasitology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Lactobacillus acidophilus Contributes to a Healthy Environment for Vaginal Epithelial Cells
Korean J Parasitol. 2011;49(3):295-298.   Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.295
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Lactobacillus acidophilus Contributes to a Healthy Environment for Vaginal Epithelial Cells
Korean J Parasitol. 2011;49(3):295-298.   Published online September 30, 2011
DOI: https://doi.org/10.3347/kjp.2011.49.3.295
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Lactobacillus acidophilus Contributes to a Healthy Environment for Vaginal Epithelial Cells
Image Image Image
Fig. 1 Analysis of pH, optical density (OD600 nm), and number of colony forming units (CFUs) from cultures of L. acidophilus with or without MS74 cells. To assess the ability of L. acidophilus to maintain a low pH under different culture conditions, L. acidophilus was cultured with or without MS74 cells in the basal medium. The pH measurements were performed 0, 3, 6, 9, 12, and 24 hr after incubation. (A) Measurements of the pH of bacterial suspensions before and after culture, (B) Measurements of the OD600 nm of bacterial suspensions, (C) Measurements of CFUs to assess the viability of L. acidophilus. CFUs are presented as logarithmic values. MS74 cells, immortalized human vaginal epithelial cells; OD600 nm, optical density; CFU, colony forming unit;-○-, MS74 cells alone;-▴-, MS74 cells co-cultured with L. acidophilus;-□-, L. acidophilus alone. Each value represents the mean±SD from 3 independent experiments, with each condition tested in triplicate.
Fig. 2 Microscopic findings of MS74 cells cultured with or without L. acidophilus after a 24-hr incubation. (A) MS74 cells cultured without L. acidophilus. (B) MS74 cells co-cultured with L. acidophilus (×400).
Fig. 3 Viable cell numbers of MS74 cells cultured with or without L. acidophilus after a 24-hr incubation. MS74, MS74 cells alone; MS74 + Lacto, MS74 cells co-cultured with L. acidophilus. Each value represents the mean±SD from 3 independent experiments. Statistical analysis between groups was done using the Student's t-test, *P=0.046.

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Lactobacillus acidophilus Contributes to a Healthy Environment for Vaginal Epithelial Cells
PHD : Parasites, Hosts and Diseases

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